Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,904,2120GA100.0% 1773.3 / ‑6.7 482intergenic (+838/+535)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (244/229);  minor base C (1/3);  total (246/236)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 483 total aligned reads displayed.

GTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTT  >  NC_000913/2904065‑2904356
                                                                                                                                                   |                                                                                                                                                
gTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGAGCACGGAATCCAAAGCCGAGTATCTGCGCGGTTTATCCCCGCTGacg                                                                                                                                                >  2:5417188/1‑150 (MQ=255)
  cAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGAcgcg                                                                                                                                              <  1:1000478/150‑1 (MQ=255)
  cAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGAcgcg                                                                                                                                              >  1:1246873/1‑150 (MQ=255)
   aGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTAGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGACGTGTATCTGCTCGGTTTATACCCGCTGACGCgg                                                                                                                                             >  1:2322988/1‑150 (MQ=255)
        ccGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAac                                                                                                                                        >  1:3872888/1‑150 (MQ=255)
        ccGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTGTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGTGGGGAac                                                                                                                                        >  2:5541480/1‑150 (MQ=255)
        ccGGTTTATCCCCGCGGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGGCCACGGGCGCACGGAATCCCCAGCCGTGTATCTGCGCGGTTTATCCCCGCTGCCGCggggtcc                                                                                                                                        >  1:3416065/1‑147 (MQ=255)
         cGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAaca                                                                                                                                       >  2:2727419/1‑150 (MQ=255)
         cGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAaca                                                                                                                                       <  2:4276241/150‑1 (MQ=255)
          ggTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac                                                                                                                                      >  1:2290117/1‑150 (MQ=255)
           gTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACt                                                                                                                                     <  1:250017/150‑1 (MQ=255)
             ttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTgg                                                                                                                                   >  1:4321000/1‑150 (MQ=255)
             ttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGGTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTCTCTGCTCGGTTTATCCCCGCTGACGCGGGGAGCACTgg                                                                                                                                   >  1:5009462/1‑150 (MQ=255)
               aTCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGct                                                                                                                                 >  1:262493/1‑150 (MQ=255)
                  cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTg                                                                                                                              <  2:3011529/150‑1 (MQ=255)
                  cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTg                                                                                                                              <  2:4475627/150‑1 (MQ=255)
                   ccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGc                                                                                                                             >  2:4851349/1‑150 (MQ=255)
                   ccGCTGGCGCGGGGAACACCTGTTATCGCAAATCTATGGACTATAGCTATTAGGTTTATCCACGCTGGCGCGCGGAACACTCGCAACAGCAAAACAAATCAGTGTATCTGCTCTGGTTATCACCGCAGACGCGGGGAACACAGGctaggc                                                                                                                             >  2:871625/1‑146 (MQ=255)
                   ccGCTGGCGCGGGGAACACCATATTGCGCAAATCTATGTACTATATCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGAGCACGTAATTCGATGGCGAGTAGATGGTCGGTTGATCCCCGCTGACGCGGGGAACACTGGCTCGGc                                                                                                                             >  2:3146775/1‑150 (MQ=255)
                     gCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCaa                                                                                                                           <  1:5493305/150‑1 (MQ=255)
                       tGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAca                                                                                                                         <  2:1727711/150‑1 (MQ=255)
                        ggCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcag                                                                                                                        <  2:1977559/150‑1 (MQ=255)
                        ggCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcag                                                                                                                        <  1:2728895/150‑1 (MQ=255)
                         gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagc                                                                                                                       <  2:3389260/150‑1 (MQ=255)
                           gcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagcag                                                                                                                     >  1:423957/1‑150 (MQ=255)
                                gAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAccc                                                                                                                <  1:901719/150‑1 (MQ=255)
                                  acacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAt                                                                                                              >  1:4548915/1‑150 (MQ=255)
                                  acacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAt                                                                                                              <  2:1142498/150‑1 (MQ=255)
                                    acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGACTACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTGCAACAGCAGCACCCATGa                                                                                                            >  2:482201/1‑150 (MQ=255)
                                     ccTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGAc                                                                                                           <  1:3451008/150‑1 (MQ=255)
                                      cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGAcc                                                                                                          >  2:561096/1‑150 (MQ=255)
                                       tGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCa                                                                                                         >  2:1874963/1‑150 (MQ=255)
                                        gTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCAc                                                                                                        <  1:1404027/150‑1 (MQ=255)
                                            tCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCg                                                                                                    >  1:3904955/1‑150 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAATCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  <  2:371795/150‑1 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  >  2:561335/1‑150 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTACTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  <  2:4062301/150‑1 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGAGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  >  2:361427/1‑150 (MQ=255)
                                                     tATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATccc                                                                                           >  1:3071312/1‑150 (MQ=255)
                                                      aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAATCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGGTTATcccc                                                                                          >  2:650964/1‑150 (MQ=255)
                                                      aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACCACGGTTTATcccc                                                                                          >  1:3266913/1‑150 (MQ=255)
                                                       tGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCg                                                                                         <  1:4851349/150‑1 (MQ=255)
                                                       tGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCg                                                                                         >  1:5311256/1‑150 (MQ=255)
                                                        ggACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGc                                                                                        <  1:2984445/150‑1 (MQ=255)
                                                        ggACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGc                                                                                        <  1:4702138/150‑1 (MQ=255)
                                                          aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTg                                                                                      >  2:1633237/1‑150 (MQ=255)
                                                                gCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgg                                                                                <  1:783584/150‑1 (MQ=255)
                                                                gCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgg                                                                                <  1:584860/150‑1 (MQ=255)
                                                                 cTATTCGGTTTATCCCCGCTGGCGCGGGGAACCCGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                               <  2:262493/150‑1 (MQ=255)
                                                                 cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                               >  1:3724270/1‑150 (MQ=255)
                                                                 cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                               <  2:148309/150‑1 (MQ=255)
                                                                     tCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAac                                                                           >  1:4436678/1‑150 (MQ=255)
                                                                       ggTTTATCCCCGCTGGCGCGGTGAACACGGGCGCACGGAATACAAAGCCGTGTATCTTCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAacac                                                                         <  1:167574/150‑1 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAAAGCTg                                                                >  1:4624335/1‑150 (MQ=255)
                                                                                 cGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTgg                                                               >  2:508711/1‑150 (MQ=255)
                                                                                 cGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTgg                                                               <  2:423957/150‑1 (MQ=255)
                                                                                      gcgcGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGc                                                          <  2:5799545/150‑1 (MQ=255)
                                                                                      gcgcGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGc                                                          <  1:5188016/150‑1 (MQ=255)
                                                                                       cgcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCg                                                         >  1:95821/1‑150 (MQ=255)
                                                                                         cgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGtt                                                       <  1:1296532/150‑1 (MQ=255)
                                                                                          ggggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGttt                                                      >  1:2200681/1‑149 (MQ=255)
                                                                                            ggAACTCGGGCGCTCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAAt                                                    <  1:5212698/150‑1 (MQ=255)
                                                                                            ggAACACGGTCGCACGGAATAAAAAGCCGTGTATCTTCTCGGTTTATCCCAGCTGACGCGGGTAACACTGTCTCTGAAACAGCTGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAAt                                                    <  2:1330595/150‑1 (MQ=255)
                                                                                            ggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAAt                                                    <  1:3560999/150‑1 (MQ=255)
                                                                                               acacGGGCGCACGGAATACAACGCCGTGTATCTGCTCGGTTTAGCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                                 <  1:1021386/150‑1 (MQ=255)
                                                                                               acacGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                                 >  2:1030401/1‑150 (MQ=255)
                                                                                                cacGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg                                                <  1:2769179/150‑1 (MQ=255)
                                                                                                 acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGc                                               <  2:2063232/150‑1 (MQ=255)
                                                                                                 acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGc                                               >  1:528441/1‑150 (MQ=255)
                                                                                                     gcgcACGGAATACAAAGCCGGGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGGTTATCCCCGCTGGCGCGGGGACCACGAAATGCTGGTGAGCGTTAAGGCCGCAAac                                           >  2:5272876/1‑150 (MQ=255)
                                                                                                       gcACGGAATCCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAacac                                         <  2:2500869/150‑1 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       >  2:3080516/1‑150 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       >  2:3098627/1‑150 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       <  2:2112150/150‑1 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       >  2:5479907/1‑150 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       <  2:3960899/150‑1 (MQ=255)
                                                                                                         aCGGAATACAAAGCAGTGTATCTGCTCGGTGTATCCCCTCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTAACCCCGCAGGCGCGGGGACCACGACATGCTGGAGAGCGTTAATGCCGAAAacaccg                                       >  2:2351480/1‑148 (MQ=255)
                                                                                                          cGGAATTCTAAGGCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACGGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                      <  2:1239045/150‑1 (MQ=255)
                                                                                                          cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                      <  1:3340181/150‑1 (MQ=255)
                                                                                                          cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                      <  1:561032/150‑1 (MQ=255)
                                                                                                            gAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGtt                                    >  1:405865/1‑150 (MQ=255)
                                                                                                             aaTACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGttt                                   >  1:3922328/1‑150 (MQ=255)
                                                                                                               tACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTAt                                 >  2:4526777/1‑150 (MQ=255)
                                                                                                               tACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTAt                                 <  2:591719/150‑1 (MQ=255)
                                                                                                                       cGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTg                         >  1:5255297/1‑150 (MQ=255)
                                                                                                                         tgtATCCGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGACCACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgc                       >  2:3472574/1‑150 (MQ=255)
                                                                                                                             tCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgg                   >  1:1881246/1‑150 (MQ=255)
                                                                                                                             tCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgg                   <  1:1537936/150‑1 (MQ=255)
                                                                                                                              cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCCCCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                  <  2:3872965/150‑1 (MQ=255)
                                                                                                                               tGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTACTGCCGCAAACACAGGGTTATCCCCGCTGGCGCgggg                 >  2:4693352/1‑150 (MQ=255)
                                                                                                                                 cTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa               <  2:5341471/150‑1 (MQ=255)
                                                                                                                                 cTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa               >  1:2057425/1‑150 (MQ=255)
                                                                                                                                 cTCGGTGTCGCCCCGCTGACGCGGGGACCACTGGCTCAGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGATCACGAAATGCTGGTGAGCGTTTATGCCGCAAACTCAGGTTTATCCCCGCTGGCGCGGGGaa               <  1:4484635/150‑1 (MQ=255)
                                                                                                                                  tCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAac              <  2:2887604/150‑1 (MQ=255)
                                                                                                                                      tttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACAt          >  2:3519989/1‑150 (MQ=255)
                                                                                                                                       ttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACAtt         <  1:650964/150‑1 (MQ=255)
                                                                                                                                             ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACTCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCt   >  1:33888/1‑150 (MQ=255)
                                                                                                                                             ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACTCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCt   >  1:5782027/1‑150 (MQ=255)
                                                                                                                                              cGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCtt  >  1:3313850/1‑150 (MQ=255)
                                                                                                                                              cGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCAGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCtt  >  1:1148014/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTT  >  NC_000913/2904065‑2904356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: