Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,722 T→A 10.3% intergenic (‑102/+3) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7220TA10.3% 858.3 / 14.1 302intergenic (‑102/+3)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base T (86/172);  new base A (39/2);  total (128/174)
Fisher's exact test for biased strand distribution p-value = 1.10e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

TGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATC  >  NC_000913/3491574‑3491871
                                                                                                                                                    |                                                                                                                                                      
tGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATc                                                                                                                                                       <  2:1675910/150‑1 (MQ=255)
 gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGTGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCa                                                                                                                                                      <  1:1433806/150‑1 (MQ=255)
  aaGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGGAATCAt                                                                                                                                                     >  1:360404/1‑150 (MQ=255)
      tCCCCGTGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGCTGCCTCCCTGAAGGTGTCAATTTGCCACAATTATACGCGGCAAATACGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACg                                                                                                                                                 <  1:2559111/150‑1 (MQ=255)
       cccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACgg                                                                                                                                                <  2:175014/150‑1 (MQ=255)
         cAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCa                                                                                                                                              <  2:2205044/150‑1 (MQ=255)
                 ggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTa                                                                                                                                      >  1:3430737/1‑150 (MQ=255)
                 ggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTa                                                                                                                                      <  1:3986367/150‑1 (MQ=255)
                  gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAGATTTCCCGCGCGCGCAAACAATAAGGCCGGACTTTAg                                                                                                                                     >  2:75635/1‑150 (MQ=255)
                   ccTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACACGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGc                                                                                                                                    <  1:4415541/149‑1 (MQ=255)
                     ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACATGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGAGAAAAAATTAACGCAGGACATTATCgg                                                                                                                                  >  2:4510503/1‑150 (MQ=255)
                     ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCCCGAAAACCTTAAGGCCGGACATTAGCgg                                                                                                                                  >  2:4444399/1‑150 (MQ=255)
                     ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                                  >  2:1924943/1‑150 (MQ=255)
                       ttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGCCGATTTGCCCCGCGAGTAAACATTAAGGCCGGGCTTTTGCgggt                                                                                                                                >  2:5544035/1‑148 (MQ=255)
                        tGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGACAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGaaa                                                                                                                               <  1:3664358/150‑1 (MQ=255)
                           ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTCCCGCGCGCGCAAACAATACCGCAGGGCTAGAGCGGGAAGGa                                                                                                                            >  2:3552065/1‑150 (MQ=255)
                           ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAAACATTAAGGCAGGACTTTAGCGGACAGGa                                                                                                                            >  2:5666764/1‑150 (MQ=255)
                           ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                                            <  1:5368127/150‑1 (MQ=255)
                            tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCCCCCGAGAAAACAATACGGCAGGACGTTAGCGGAAAGGAt                                                                                                                           >  2:3017395/1‑150 (MQ=255)
                            tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGCGGAAACATTAAGGCAGGCCGTTTGCGGAAAGGAt                                                                                                                           >  2:1246502/1‑150 (MQ=255)
                              tCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATaa                                                                                                                         <  2:656033/150‑1 (MQ=255)
                                 gTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGCGAAATCAGTAAGGCCGGCCGTTAGCGGAAAGGCTAACTa                                                                                                                     >  2:4249580/1‑150 (MQ=255)
                                  tGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAACATTGCCCGCGCGAGAAATAAATAAGGCAGGACATTAGCGGAAAGGATAACGAc                                                                                                                    >  1:1599112/1‑150 (MQ=255)
                                      tttCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCCCGAGAAATCAATACGGCAGGACTTTAGCGGAAAGGATAACTACCggg                                                                                                                >  2:1346628/1‑149 (MQ=255)
                                      tttCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAACATTGCCCGCGCGAGAAATCATTACCGCAGGACATTAGCGGAAAGGATAACAACCGGt                                                                                                                >  2:5184153/1‑150 (MQ=255)
                                          ttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCAAGATTGCCCGCGCGGGAAAACAATAAGGCAGGACATTTTCGGAAAGGATAACTACCGGGTTTg                                                                                                            >  2:3987832/1‑150 (MQ=255)
                                          ttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAAGGTTTCCCGCGCGCGAAATAAATACGGCCCGACTTTAGCGGCAAGGATAACAACCCGTGTTg                                                                                                            >  1:4100454/1‑150 (MQ=255)
                                           tcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCCCATTGCCCGCGCGCGCAATAAATAACGCAGGGCGTTAGCGGGAAGGGTAAAAAACGGGTTTgt                                                                                                           >  1:535309/1‑149 (MQ=255)
                                           tcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGGTTGGCCCCCCGCGAAATAAATAAGGCCGGACGTTTTCGGGAAGGGTTACAAACCGGtttgt                                                                                                            >  1:2234876/1‑148 (MQ=255)
                                            cACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGAc                                                                                                          <  2:1084501/150‑1 (MQ=255)
                                             aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                                         <  1:4560549/150‑1 (MQ=255)
                                                 gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAAGATTGCCCGCGCGAGAAATCAATAAGGCCGGGCGTTAGCGGGAAGGGGAACTGCCCGTTTTGTCGGccc                                                                                                     >  1:3349337/1‑148 (MQ=255)
                                                    gCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGGTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAtt                                                                                                  >  2:4308931/1‑150 (MQ=255)
                                                     ccTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTc                                                                                                 <  1:2376915/150‑1 (MQ=255)
                                                      cccccTGAACGTGTCCATTTGCCACAAGTATACGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCt                                                                                                <  1:4934194/148‑1 (MQ=255)
                                                      cTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCCGAGTTCCCCCCCGCGGAATCAGTAAGGCAGGGCGTTTGAGGCAAGGGTAAAGACCCGGTTTTTCGGCACATTCt                                                                                                >  1:726871/1‑150 (MQ=255)
                                                      cTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAATTCCCCCGCGAGGCAAGAATTCGGCAGGACATTAGCCGAAAGGATAACAACCCGGGTTGACCGCACAATCt                                                                                                >  2:3195946/1‑150 (MQ=255)
                                                       tCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTg                                                                                               <  2:2914138/150‑1 (MQ=255)
                                                       tCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTg                                                                                               <  2:5482414/150‑1 (MQ=255)
                                                       acccTGAACGTGTCCAATTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAATCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTg                                                                                               <  2:2778949/149‑1 (MQ=255)
                                                        cccTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTGCCGCGCGAGAAATCATTTAGGCAGGTCTTTAGCGGTAAGGATAACTACCGGGGTTGACGGCACAATCTgg                                                                                              >  1:3242582/1‑150 (MQ=255)
                                                         ccTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCACGATTTCCCGCGCGCGAAATAAATAAGGCCCGCCTTTAGCGGCAAGGGTAACAACCCGGTTTGACCGCCCCTTCTGGt                                                                                             >  2:1792546/1‑150 (MQ=255)
                                                             aaCGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACgg                                                                                         <  1:880001/150‑1 (MQ=255)
                                                               cGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCCGATTTCCCGCGCGCGAAAACAATAAGGCAGGACAATTTCGGAAAGGATAAAAAACGGGTTTTACGGCCCAATCAGGTTCgggc                                                                                       >  2:5384159/1‑148 (MQ=255)
                                                               cGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAc                                                                                       <  2:3123792/150‑1 (MQ=255)
                                                               cGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAc                                                                                       <  2:1015753/150‑1 (MQ=255)
                                                                   tCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAAc                                                                                   <  2:5239022/150‑1 (MQ=255)
                                                                     cATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCCCCCGAGAAAACAATACGGCAGGACTTTAGCGGAAAGGATAAAAACCGGTTTTTACGGCCAAATCTGGTACGGACCAACGt                                                                                 >  2:3539422/1‑150 (MQ=255)
                                                                      aTTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTc                                                                                <  2:3318896/150‑1 (MQ=255)
                                                                          gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGa                                                                            <  2:1213756/150‑1 (MQ=255)
                                                                          gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGa                                                                            <  2:1452035/150‑1 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGTAGAAGCGCGGGCCAGAATGCCCGCCCGCGCAAGCAATAAGGCCCGGCATTAGCGGGAAGGATAACAACCGGTTTTTTCGGCCCATTCTGGTACGGACCAACCTCCTGAGTc                                                                         >  1:801754/1‑150 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGTCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTc                                                                         >  1:5650669/1‑150 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTc                                                                         <  1:5712324/150‑1 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTc                                                                         <  1:437096/150‑1 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTc                                                                         <  1:2940865/150‑1 (MQ=255)
                                                                             acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCAGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTc                                                                         >  2:2305889/1‑150 (MQ=255)
                                                                              caAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCg                                                                        <  2:5634704/150‑1 (MQ=255)
                                                                               aaGTATAAGCGGCAAATCCGGGTTACGCCAGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCgg                                                                       <  2:728990/150‑1 (MQ=255)
                                                                                 gTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCa                                                                     <  2:909409/150‑1 (MQ=255)
                                                                                  tataAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCAc                                                                    <  1:163275/150‑1 (MQ=255)
                                                                                    taAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCAAGATTGCCCCCGCGGGACAACATTAAGGCAGGCCTTTAGCGGAAAGGGTAACAAACCGTTTTTTCGGCACAAACTGGTACGGACCAACGGCAAGAGTCGGCAccg                                                                  >  2:1070183/1‑149 (MQ=255)
                                                                                    taAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTGCCCCCCGCGAAATCAATAACGCAGGACGTTATCGGGAAGGGAAACAAACGCTTTTTAAGGCACAGTCTGGTACGGACCAACCTCAATAGTCGGCAccg                                                                  >  2:1527427/1‑149 (MQ=255)
                                                                                     aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCAAGATTGACCGCGCGAGAAAACATTACCGCACGACTTTTGCGGAAAGGGTAACAAACGGTTTTGTAGGCACATTCATGTACGGACCAAACTCAGGAGTCGGCAccgg                                                                 >  2:61449/1‑148 (MQ=255)
                                                                                     aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAATGGCCCCCCGCGAAATAAATACGGCACGACGTTTTCCGAAAGGATAAAAACCGGGTTTTAAGGCCCAATATGGTACGGACCAAACTCATGAGTAGGCAcccg                                                                 >  2:3458579/1‑148 (MQ=255)
                                                                                      aGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTgg                                                                <  2:4829095/150‑1 (MQ=255)
                                                                                      aGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTgg                                                                <  1:5544035/150‑1 (MQ=255)
                                                                                        cGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAGATTGCCCGCGCGAGAAATCATTAAGGCAGGACGTTATCGGGAAGGATAACAACCCGGTTTTTCGGCACAATCTGGTACGGCCCAACGTCAAGAGTCGGCCCCCGGGa                                                              >  2:778755/1‑150 (MQ=255)
                                                                                           cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                                           <  1:2160257/150‑1 (MQ=255)
                                                                                           cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                                           <  1:4187469/150‑1 (MQ=255)
                                                                                           cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                                           <  1:4550522/150‑1 (MQ=255)
                                                                                               tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                                       <  2:2412103/150‑1 (MQ=255)
                                                                                                ccGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt                                                      <  2:2628491/150‑1 (MQ=255)
                                                                                                ccGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt                                                      <  2:698749/150‑1 (MQ=255)
                                                                                                 cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGTTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                                     <  1:2848671/150‑1 (MQ=255)
                                                                                                  gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCg                                                    <  1:1867263/150‑1 (MQ=255)
                                                                                                    gTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGc                                                  <  1:1165329/150‑1 (MQ=255)
                                                                                                         gCCGGGAGAGCAGAAGCGCGGGCCAGCTTGCCCCCGCGAGCCATCAGTACGGCCCGACGTTAGCCGACAGGATAACAACCCGTGTTGACGGCCCAAGAGGGTCCGGACCACCCGCAAGAGTCGGCCCCGGGGCACGCGTATCGGccccgg                                             >  2:4078190/1‑145 (MQ=255)
                                                                                                          ccGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTc                                            <  1:5255637/150‑1 (MQ=255)
                                                                                                               agagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGc                                       <  1:4802701/150‑1 (MQ=255)
                                                                                                                    aGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTAGGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc                                  <  1:4738682/150‑1 (MQ=255)
                                                                                                                    aGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc                                  <  2:3578222/150‑1 (MQ=255)
                                                                                                                        gcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca                              <  2:4941331/150‑1 (MQ=255)
                                                                                                                        gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca                              <  2:2368545/150‑1 (MQ=255)
                                                                                                                        gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca                              <  2:3612994/150‑1 (MQ=255)
                                                                                                                        gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca                              <  2:34089/150‑1 (MQ=255)
                                                                                                                        gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAAGCCTTTaaccc                              >  1:4047118/1‑149 (MQ=255)
                                                                                                                             ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa                         >  1:5054686/1‑150 (MQ=255)
                                                                                                                              gCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAc                        >  1:2445616/1‑150 (MQ=255)
                                                                                                                               cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc                       >  2:2714965/1‑150 (MQ=255)
                                                                                                                                  gATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCaaa                    >  1:2686376/1‑150 (MQ=255)
                                                                                                                                  gATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCaaa                    <  2:1125141/150‑1 (MQ=255)
                                                                                                                                            gcgAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTaa          >  2:2062469/1‑150 (MQ=255)
                                                                                                                                             cgAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAAc         >  1:30811/1‑150 (MQ=255)
                                                                                                                                             cgAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAAc         <  1:1070125/150‑1 (MQ=255)
                                                                                                                                              gagaAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAAcc        <  1:545442/150‑1 (MQ=255)
                                                                                                                                               agaAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCg       <  1:3799741/150‑1 (MQ=255)
                                                                                                                                                 aaaTCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGaa     <  2:3889085/150‑1 (MQ=255)
                                                                                                                                                 aaaTCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGaa     <  1:5098508/150‑1 (MQ=255)
                                                                                                                                                    tCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATc  <  1:3521591/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATC  >  NC_000913/3491574‑3491871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: