Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,421,328 T→G 5.1% L450R (CTG→CGG)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,421,3280TG 5.1% 1397.5 / 2.8 501L450R (CTG→CGG) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base T (178/277);  new base G (29/11);  total (213/288)
Fisher's exact test for biased strand distribution p-value = 7.59e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 505 total aligned reads displayed.

ACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAACC  >  NC_000913/4421179‑4421476
                                                                                                                                                     |                                                                                                                                                    
aCTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCt                                                                                                                                                      <  2:516255/150‑1 (MQ=255)
aCTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCt                                                                                                                                                      <  2:2622107/150‑1 (MQ=255)
  tCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                    >  2:2329894/1‑150 (MQ=255)
  tCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                    <  2:3045522/150‑1 (MQ=255)
  tCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgc                                                                                                                                                    >  1:887727/1‑150 (MQ=255)
  tCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGgc                                                                                                                                                    >  1:1002841/1‑150 (MQ=255)
   cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                                                                                   >  2:4920341/1‑150 (MQ=255)
             aGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaa                                                                                                                                         >  2:408928/1‑150 (MQ=255)
              gTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaag                                                                                                                                        <  2:4166839/150‑1 (MQ=255)
               tGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCcgaagaaga                                                                                                                                       >  2:706661/1‑150 (MQ=255)
               tGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTACTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaaga                                                                                                                                       <  2:2101549/150‑1 (MQ=255)
                 ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATg                                                                                                                                     >  2:3100780/1‑150 (MQ=255)
                 ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCATATATGTTATTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATg                                                                                                                                     >  1:2464831/1‑150 (MQ=255)
                  cTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGc                                                                                                                                    >  2:562554/1‑150 (MQ=255)
                  cTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCCGAAGAAGATGc                                                                                                                                    >  1:2646462/1‑150 (MQ=255)
                       tGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGaaa                                                                                                                               <  1:4893381/150‑1 (MQ=255)
                       tGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGaaa                                                                                                                               <  2:1006388/150‑1 (MQ=255)
                          gCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaac                                                                                                                            <  1:4000265/150‑1 (MQ=255)
                               gCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTg                                                                                                                       >  2:1347233/1‑150 (MQ=255)
                               gCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTATTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATTCAGAAAAAAAAATg                                                                                                                       >  1:1968686/1‑150 (MQ=255)
                                cGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                                                                                      <  2:1081836/150‑1 (MQ=255)
                                   tgggttCGATTCTGGCACCGCCGATGATGCATGGCTTCTTCTCCATCGTTATCGCCTTTTTGGCCGTCATCATTTTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAg                                                                                                                   <  1:124346/145‑1 (MQ=255)
                                     tGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGaa                                                                                                                 <  2:4445923/150‑1 (MQ=255)
                                     tGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCAACGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTATTAGCTGGCCGCGCGCGGCGCGCAGAAGAAGATTCAGAAAAAAAACAGGCAGaa                                                                                                                 >  1:2381901/1‑150 (MQ=255)
                                       gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACa                                                                                                               >  2:3720437/1‑150 (MQ=255)
                                       gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACAGGCAGAACa                                                                                                               >  1:4377038/1‑150 (MQ=255)
                                       gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATAGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTATTCGCTGGCCGCGCGCGGCGCGCCGAAGAAGATGCAGAAAAACAACAGGCACaaaa                                                                                                               >  1:1092545/1‑148 (MQ=255)
                                            ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTg                                                                                                          >  1:3088664/1‑150 (MQ=255)
                                            ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTg                                                                                                          <  1:500232/150‑1 (MQ=255)
                                             tCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGc                                                                                                         <  1:1694232/150‑1 (MQ=255)
                                               tGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCtt                                                                                                       <  2:4683925/150‑1 (MQ=255)
                                               tGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATAGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGGCTGCtt                                                                                                       >  2:5438358/1‑150 (MQ=255)
                                                        cgATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCTTTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                                              <  1:2949874/150‑1 (MQ=255)
                                                         gatgatGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGt                                                                                             >  1:4708438/1‑150 (MQ=255)
                                                           tgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGttt                                                                                           <  2:1957971/150‑1 (MQ=255)
                                                           tgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGttt                                                                                           <  2:2253909/150‑1 (MQ=255)
                                                            gatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtttt                                                                                          >  1:1594762/1‑150 (MQ=255)
                                                              tgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                                                                                        <  2:4338446/150‑1 (MQ=255)
                                                              tgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                                                                                        <  1:5217588/150‑1 (MQ=255)
                                                              tgCAAGGCTTATTCTCCATCGGTATCGCCTTTATGGCCTTCATCATTGTAATTGAACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                                                                                        <  1:4257841/150‑1 (MQ=255)
                                                               gCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGTAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                                                                       <  1:2867078/150‑1 (MQ=255)
                                                               gCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                                                                       <  2:3583669/150‑1 (MQ=255)
                                                               gCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                                                                       <  2:2450604/150‑1 (MQ=255)
                                                                 aaGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTa                                                                                     >  1:740623/1‑150 (MQ=255)
                                                                 aaGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTa                                                                                     >  2:297122/1‑150 (MQ=255)
                                                                 aaGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTa                                                                                     <  1:3911517/150‑1 (MQ=255)
                                                                    gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGa                                                                                  <  2:1679267/150‑1 (MQ=255)
                                                                       tcttctCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcg                                                                               <  1:5791392/150‑1 (MQ=255)
                                                                        cttctCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgt                                                                              >  2:3913422/1‑150 (MQ=255)
                                                                         ttctCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                                                                             <  1:4145430/150‑1 (MQ=255)
                                                                          tctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtac                                                                            <  1:3763347/150‑1 (MQ=255)
                                                                          tctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtac                                                                            <  2:846387/150‑1 (MQ=255)
                                                                           ctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCCGAAGAAGATGCAGAAAAACAACTGGCAGAAAAGTCGGCGTAATAAGGAGTTTTGATTATGACcgttcg                                                                           >  1:2147993/1‑150 (MQ=255)
                                                                           ctcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacg                                                                           >  1:1203338/1‑150 (MQ=255)
                                                                            tcCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacgt                                                                          >  2:2159638/1‑150 (MQ=255)
                                                                              cATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTAt                                                                        >  1:1554633/1‑150 (MQ=255)
                                                                              cATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCACAAGAAGATGCAGAAAAACAACAGGCAGTACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTAt                                                                        >  2:2554302/1‑150 (MQ=255)
                                                                               aTCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTAtt                                                                       >  2:2723592/1‑150 (MQ=255)
                                                                                 cGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCt                                                                     <  1:5642633/150‑1 (MQ=255)
                                                                                   gTATCGCCTTTCTGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                                                   <  2:3400136/150‑1 (MQ=255)
                                                                                   gTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                                                   >  2:331780/1‑150 (MQ=255)
                                                                                   gTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                                                   <  1:4642727/150‑1 (MQ=255)
                                                                                     aTCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCt                                                                 <  2:5101097/150‑1 (MQ=255)
                                                                                       cGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgt                                                               <  1:5074242/150‑1 (MQ=255)
                                                                                         ccTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgt                                                             >  2:4841020/1‑150 (MQ=255)
                                                                                          cTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATTCAGAAAAAAAACTGGCAGAACAATCAGCTTAATAAGGAGTTTTTATTATGAACGTAAGTATTCTGGCtgtgtg                                                            >  1:2084494/1‑150 (MQ=255)
                                                                                           tttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtgt                                                           <  1:1442586/150‑1 (MQ=255)
                                                                                            ttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtgtg                                                          >  2:2807665/1‑150 (MQ=255)
                                                                                               tGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCa                                                       >  2:126739/1‑150 (MQ=255)
                                                                                                ggCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCaa                                                      <  2:5326931/150‑1 (MQ=255)
                                                                                                   cGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACgg                                                   <  2:1859994/150‑1 (MQ=255)
                                                                                                    gTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGa                                                  <  1:2696734/150‑1 (MQ=255)
                                                                                                      catcatTGTATTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACa                                                <  1:5391644/150‑1 (MQ=255)
                                                                                                         catTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                                             <  2:4390719/150‑1 (MQ=255)
                                                                                                           ttGTACTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCa                                           <  2:3982476/150‑1 (MQ=255)
                                                                                                           ttGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCa                                           <  2:3088664/150‑1 (MQ=255)
                                                                                                           ttGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCa                                           >  2:425247/1‑150 (MQ=255)
                                                                                                                aTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTcc                                      <  1:3418140/150‑1 (MQ=255)
                                                                                                                 ttGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGTTGCAGTAAAACAACTGGGAGAACAGTCTGGTTAATAAGGTGTTTTGTTTATGTCCGTTCGTTTTGTGGCTGTGTGTGGGAACGGTCAAGGCAGTTCCa                                     >  2:3184187/1‑150 (MQ=255)
                                                                                                                 ttGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCa                                     <  1:3869328/150‑1 (MQ=255)
                                                                                                                  tGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCAt                                    <  2:1564047/150‑1 (MQ=255)
                                                                                                                     aCTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGAt                                 <  2:1464115/150‑1 (MQ=255)
                                                                                                                      cTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATc                                <  1:3147669/150‑1 (MQ=255)
                                                                                                                       tGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGGACGTATTCTGGCTGTGTGTGGCAACGGACCAGGCAGTTCCATGAtaa                               >  2:2791808/1‑148 (MQ=255)
                                                                                                                            tataTGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAg                          <  1:3522822/150‑1 (MQ=255)
                                                                                                                                 gTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGaa                     <  2:944662/150‑1 (MQ=255)
                                                                                                                                  ttcttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGaaa                    <  2:4755990/150‑1 (MQ=255)
                                                                                                                                   tcttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAg                   >  2:4536274/1‑150 (MQ=255)
                                                                                                                                     ttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTg                 >  2:591092/1‑150 (MQ=255)
                                                                                                                                      tcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGCCCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTgg                <  2:5205539/150‑1 (MQ=255)
                                                                                                                                        gCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGTTTTTGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGAc              <  2:1227232/150‑1 (MQ=255)
                                                                                                                                          tGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTTCTTAATAAGGATTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCAAGATCCTGAAGATGAAAGTGGACCa            >  2:5710472/1‑150 (MQ=255)
                                                                                                                                             ccGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATTACCGTACGTATTATGGCTGTGTGTGGCAACGGACAAGGCAGTTACATGATCATGAAGATGAAAGTGGACCAGtt         >  1:367155/1‑150 (MQ=255)
                                                                                                                                             ccGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATAATGAAGATGAAAGTTGACCAGtt         >  1:3314656/1‑150 (MQ=255)
                                                                                                                                             ccGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACAGGCAGAAAAGTCTGCTTAATAAGGAGTTTTTATTATGACCGTACGTATTATGGCTGTGTGTGGCAAAGGACAAGGCAGTTCCAATATCATGAAGATGAAAGTGGACCACtt         >  1:2253965/1‑150 (MQ=255)
                                                                                                                                               gcgcgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGtttt       >  1:5257438/1‑150 (MQ=255)
                                                                                                                                               gcgcgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGtttt       <  1:2807665/150‑1 (MQ=255)
                                                                                                                                                  cgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTaa    <  1:5503503/150‑1 (MQ=255)
                                                                                                                                                  cgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTaa    <  2:3610805/150‑1 (MQ=255)
                                                                                                                                                    cTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAcc  <  2:3799644/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAACC  >  NC_000913/4421179‑4421476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: