Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,596,823 A→C 5.6% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase; L‑gulonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,596,8230AC 5.6% 929.2 / 4.7 291E278D (GAA→GAClgoDL‑galactonate oxidoreductase; L‑gulonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (182/86);  new base C (2/21);  total (184/107)
Fisher's exact test for biased strand distribution p-value = 2.35e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

TCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGA  >  NC_000913/4596674‑4596971
                                                                                                                                                     |                                                                                                                                                    
tCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGaa                                                                                                                                                      <  2:2388730/150‑1 (MQ=255)
 cGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGaaa                                                                                                                                                     <  2:2551903/150‑1 (MQ=255)
  gCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAac                                                                                                                                                    >  1:1829123/1‑150 (MQ=255)
   cTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAacg                                                                                                                                                   >  2:3659153/1‑150 (MQ=255)
    tGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAacga                                                                                                                                                  >  1:1992955/1‑150 (MQ=255)
        gcAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAAGTTGATTCGTCACGGCGGCACGGTGGTCTTTGTCGGCCGGTGTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGACACGACgat                                                                                                                                              >  2:2798338/1‑150 (MQ=255)
            aaaGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGatgatg                                                                                                                                          >  1:2411344/1‑150 (MQ=255)
                  aTCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGc                                                                                                                                    >  1:5494967/1‑150 (MQ=255)
                  aTCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGc                                                                                                                                    >  1:3641790/1‑150 (MQ=255)
                   tCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGcc                                                                                                                                   >  2:2178743/1‑150 (MQ=255)
                    cGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCg                                                                                                                                  >  2:538526/1‑150 (MQ=255)
                      aCGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCa                                                                                                                                >  1:3043359/1‑150 (MQ=255)
                      aCGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCa                                                                                                                                >  1:2568279/1‑150 (MQ=255)
                         cgACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacg                                                                                                                             >  2:3172659/1‑150 (MQ=255)
                           aCAGTTAATCAACATGCTATTAATAACACCGTTATTTTGATTCGCCACGGCGGCAGGGTGGTTTTTGTCGGCCTGTTTAATGGTGTGTTGATGTTCTCCGATCCGCAATACCATAAAAAATACACGACGATGATGGGCAGCCGCAacgcc                                                                                                                           <  2:563711/150‑1 (MQ=255)
                            cAGGTAATCAACATGCGTTGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCCCCGATCCGGAATTCCATAAAAAATAAACGACGATGATGGGCAGCCGCAacgcca                                                                                                                          <  2:3405941/150‑1 (MQ=255)
                             aGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccac                                                                                                                         >  2:5814003/1‑150 (MQ=255)
                               gTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgc                                                                                                                       >  1:2109295/1‑150 (MQ=255)
                                tAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgcc                                                                                                                      >  2:4023166/1‑150 (MQ=255)
                                tAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgcc                                                                                                                      >  1:1633705/1‑150 (MQ=255)
                                 aaacAACATGCTATGAATAACACCGTTAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAACGGTGAGTTGCTGTTCTCCTATCCGTAATTCAATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCg                                                                                                                     <  2:483401/147‑1 (MQ=255)
                                  aacAACATGCGATGAATACCACCGTGAATTTGTTTCGTCACGGCGGCAGGGTGGTATTTGTGGGCCTTTTTAACGGTGATTTGCTGTTCTCCTATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCgg                                                                                                                    <  2:4527781/148‑1 (MQ=255)
                                  aTCAACCTGCTATCAATAACCCCGTGATTTTGTGTCGCCCCGGCGGCCGGGTGGTTTTTGTCGGCCTGTTTAACGGTTTGTGGCAGTTCCCCGATCCGTATTGCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCgg                                                                                                                    <  2:2961656/150‑1 (MQ=255)
                                    cAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGAGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGaa                                                                                                                  >  2:1797349/1‑150 (MQ=255)
                                      aCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGa                                                                                                                >  1:2323458/1‑150 (MQ=255)
                                         tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAATGGTGAGTTGCTGTTCTCCGATCCGGATTTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAttt                                                                                                             <  2:923290/150‑1 (MQ=255)
                                          gCGATGAATAACACGGTGATTTTGTTTGGTCACGGGGGCACGGTGGTTTTTGTCGGCCTTTTTAATGGTGAGTTGCTTTTCTCCGATCCGGATTTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAtttt                                                                                                            <  2:4224198/150‑1 (MQ=255)
                                          gCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAtttt                                                                                                            >  2:3082765/1‑150 (MQ=255)
                                           ctttGAATAACACCGTTATTTTGATTCGTCTGGGGGGCTCGTTTGTTTTTGTGGGCCTTTTTAATGGTGTGTTGCTTTTCTCCTATCCGTAATTCAATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTg                                                                                                           <  2:5544107/147‑1 (MQ=255)
                                             aTGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCt                                                                                                         >  2:4091621/1‑150 (MQ=255)
                                               gAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTaa                                                                                                       >  1:2752669/1‑150 (MQ=255)
                                                aaTAACACGTTTATTTTTATTGGTCTGGGGGGCAGGGTGTTTTTTGTGGGCCTGTTTAATGGTTTGTTGATGTTCTCCGAGCGGGAATTCCATAAAAAATACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTaaa                                                                                                      <  2:4503443/150‑1 (MQ=255)
                                                    acacCGTGAATTTGATTCGTCACGGCGGCACGGTGTTATTTGTCGGCCTGTTTAACGGTGAGTTGCTTTTCTCCTATCCGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcg                                                                                                  <  2:2299651/150‑1 (MQ=255)
                                                    acacCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcg                                                                                                  >  1:76660/1‑150 (MQ=255)
                                                     cacCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGTTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcgg                                                                                                 <  2:3260868/150‑1 (MQ=255)
                                                      acCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                                                                >  2:4344502/1‑150 (MQ=255)
                                                         gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGa                                                                                             >  2:1374375/1‑150 (MQ=255)
                                                            aaTTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTg                                                                                          >  1:3861873/1‑150 (MQ=255)
                                                              tttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGAt                                                                                        >  2:1823867/1‑150 (MQ=255)
                                                                    tCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCgga                                                                                  >  1:5123636/1‑150 (MQ=255)
                                                                    tCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCgga                                                                                  >  1:4994559/1‑150 (MQ=255)
                                                                    tCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCgga                                                                                  >  1:2234247/1‑150 (MQ=255)
                                                                    tCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCgga                                                                                  >  2:5266566/1‑150 (MQ=255)
                                                                     cGTCACGGCGGCACGGTGTTTTTTGTCGGCCTTTTTAAAGGTGTGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGAGGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaa                                                                                 <  2:4801728/150‑1 (MQ=255)
                                                                      gTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaag                                                                                >  1:882520/1‑150 (MQ=255)
                                                                       tCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaagg                                                                               >  1:5750879/1‑150 (MQ=255)
                                                                       tCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaagg                                                                               >  2:729873/1‑150 (MQ=255)
                                                                        cACGGCGGCTGGGGGTTATGTGTCGGCGTTTTTAATGGTGAGTTGCCGTTCTCCGATCCTTAATTCAAAAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaagga                                                                              <  1:1832746/150‑1 (MQ=255)
                                                                                   ggtggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgc                                                                   >  2:3210252/1‑150 (MQ=255)
                                                                                   ggtggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgc                                                                   >  2:1008692/1‑150 (MQ=255)
                                                                                    gtggtATTGGTCGGCCTGTTTAAAGTTGAGTTGCATTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgct                                                                  <  2:3115501/150‑1 (MQ=255)
                                                                                         aTTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACat                                                             >  1:4852678/1‑150 (MQ=255)
                                                                                            tGTCGGCCTTTTTACTGGTGTGTTGCTTTTCCCCGATCCGTAATCCAAAAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                                                          <  1:1161871/150‑1 (MQ=255)
                                                                                            tGTCGGCCTTTTTAATGGTGATTTGCAGTACTCCGAACCGGAATCCAAAAAAAAACAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                                                          <  2:111849/150‑1 (MQ=255)
                                                                                            tGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                                                          >  1:5630204/1‑150 (MQ=255)
                                                                                             gTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgatg                                                         <  2:4769579/150‑1 (MQ=255)
                                                                                              tCGGCCTTTTTAAAGTTGAGTTGCATTTCTCCGATCCGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGt                                                        <  2:789513/150‑1 (MQ=255)
                                                                                              tCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGt                                                        >  2:1218740/1‑150 (MQ=255)
                                                                                              tCGGCATGTTTAACGGTGAGTTGCAGTTCTCCTATCCTTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGt                                                        <  1:3268227/150‑1 (MQ=255)
                                                                                                 gCCTTTTTAAAGGTTATTTGAAGTTCTCCTATCCGGAATTCAATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTaa                                                     <  2:1601896/150‑1 (MQ=255)
                                                                                                  ccTTTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                    <  2:4261707/150‑1 (MQ=255)
                                                                                                  ccTGTTTAACGGTGCGTTGCAGTTCGCCGAGCCGGAATGCCATAAAAAAGAAACGACGCTGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                    <  2:837484/150‑1 (MQ=255)
                                                                                                  ccTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                    <  2:3358784/150‑1 (MQ=255)
                                                                                                   cTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAcc                                                   >  1:1693939/1‑150 (MQ=255)
                                                                                                        tAATGGTGAGTTGCAGTTCTCCGATCCTTAATTCAATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCg                                              <  2:5142013/150‑1 (MQ=255)
                                                                                                        tAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCg                                              >  1:4798424/1‑150 (MQ=255)
                                                                                                           aGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTa                                           >  1:2496362/1‑150 (MQ=255)
                                                                                                             gTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATc                                         <  1:3415505/150‑1 (MQ=255)
                                                                                                             gTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATc                                         >  1:2819449/1‑150 (MQ=255)
                                                                                                             gTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATAGCTATc                                         >  1:4237423/1‑150 (MQ=255)
                                                                                                             gTGAGTTGCAGTTCTCCGATCCGGAATTCAATAAAAAATACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATc                                         <  1:471997/150‑1 (MQ=255)
                                                                                                                aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGACGATTTTGCTAAAGTCGGTCGACTGCTGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGt                                      >  2:2775902/1‑150 (MQ=255)
                                                                                                                  ttGCATTTCTCCGATCCGGAATTCCAAAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTc                                    <  2:3518708/150‑1 (MQ=255)
                                                                                                                  ttGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTc                                    >  2:3545286/1‑150 (MQ=255)
                                                                                                                  ttGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTc                                    >  2:4883922/1‑150 (MQ=255)
                                                                                                                  ttGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTc                                    >  2:3114282/1‑150 (MQ=255)
                                                                                                                   tGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCg                                   >  1:4930017/1‑150 (MQ=255)
                                                                                                                    gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGc                                  >  1:5694806/1‑150 (MQ=255)
                                                                                                                    gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGc                                  >  1:5282755/1‑150 (MQ=255)
                                                                                                                       gTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCAc                               <  2:4795145/150‑1 (MQ=255)
                                                                                                                           tcCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTg                           >  1:2699589/1‑150 (MQ=255)
                                                                                                                           tcCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTg                           >  1:1598205/1‑150 (MQ=255)
                                                                                                                           tcCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTg                           >  1:5564650/1‑150 (MQ=255)
                                                                                                                            ccGACCCGTAATTCAATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTgg                          <  1:3832653/150‑1 (MQ=255)
                                                                                                                             cGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGc                         <  2:877879/150‑1 (MQ=255)
                                                                                                                             cGAGCCGTAATTCAAAAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGc                         <  1:1084099/150‑1 (MQ=255)
                                                                                                                              gagccGGATTTCAATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCa                        <  2:392776/147‑1 (MQ=255)
                                                                                                                                   gtaaTTCCATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAAc                   <  1:1269649/148‑1 (MQ=255)
                                                                                                                                   ggAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAAc                   >  2:5060685/1‑150 (MQ=255)
                                                                                                                                     aaTTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCt                 >  1:557989/1‑150 (MQ=255)
                                                                                                                                         ccATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGa             <  1:5814003/150‑1 (MQ=255)
                                                                                                                                          cATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAg            >  1:1852920/1‑150 (MQ=255)
                                                                                                                                           aTAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAgc           >  1:1752706/1‑150 (MQ=255)
                                                                                                                                           aTAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAgc           >  2:2039983/1‑150 (MQ=255)
                                                                                                                                            tAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAgcg          >  2:4708170/1‑150 (MQ=255)
                                                                                                                                             aaaaaaGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAgcgc         >  1:5205380/1‑150 (MQ=255)
                                                                                                                                               aaacgaaacgACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGa       <  2:2574232/150‑1 (MQ=255)
                                                                                                                                               aaaaGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGa       >  1:2153102/1‑150 (MQ=255)
                                                                                                                                                    aaaCGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGTTGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGa  <  2:3836865/150‑1 (MQ=255)
                                                                                                                                                    aaaCGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGa  >  2:1522921/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGA  >  NC_000913/4596674‑4596971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: