Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913222,9580A.96.9% 412.6 / 5.9 97coding (126/576 nt)gmhBD,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base . (48/46);  total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 106 total aligned reads displayed.

CCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCT  >  NC_000913/222811‑223100
                                                                                                                                                   |                                                                                                                                              
ccTTATTAAGCAGCAAAAAAACCTGCCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGCCGGTGTTATTGACGCCATGCGCGAGCTaa                                                                                                                                               <  2:779152/149‑1 (MQ=255)
   tattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATTGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTCTTGACGCCATGCGCGAGCTaaaaa                                                                                                                                            <  1:429108/149‑1 (MQ=255)
   tattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaa                                                                                                                                            >  2:781406/1‑149 (MQ=255)
   tattaAGGAGCGATAAAAGGGGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATCGCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaa                                                                                                                                            <  2:1007547/149‑1 (MQ=255)
    attaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGAGAGCTaaaaaa                                                                                                                                           >  1:1218638/1‑149 (MQ=255)
      taAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                         >  2:79035/1‑148 (MQ=255)
        aGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaatgg                                                                                                                                       >  1:1421690/1‑146 (MQ=255)
         ggAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaatggg                                                                                                                                      >  1:191192/1‑145 (MQ=255)
               ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgc                                                                                                                               >  1:508015/1‑149 (MQ=255)
               ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgc                                                                                                                               >  1:1146839/1‑149 (MQ=255)
                taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcg                                                                                                                              >  1:1368700/1‑149 (MQ=255)
                taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcc                                                                                                                              >  1:1086851/1‑148 (MQ=255)
                 aaaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcgc                                                                                                                             <  1:800463/149‑1 (MQ=255)
                      gTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtggtg                                                                                                                        <  1:786144/149‑1 (MQ=255)
                       tGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGATATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtggtgg                                                                                                                       >  2:1103090/1‑149 (MQ=255)
                        ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGCCACCTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAACAATGGGCTTTGCGCTggtggt                                                                                                                      >  1:1534028/1‑149 (MQ=255)
                        ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTggtggt                                                                                                                      <  2:191192/149‑1 (MQ=255)
                         gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTggtggtt                                                                                                                     >  1:151470/1‑148 (MQ=255)
                         gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                     >  1:1174687/1‑149 (MQ=255)
                          cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtag                                                                                                                    >  2:341461/1‑149 (MQ=255)
                           gAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagt                                                                                                                   >  1:693043/1‑149 (MQ=255)
                            aaGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagta                                                                                                                  <  1:803911/149‑1 (MQ=255)
                            aaGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagta                                                                                                                  <  1:396335/149‑1 (MQ=255)
                             agagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaa                                                                                                                 <  2:1212843/149‑1 (MQ=255)
                              gagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaac                                                                                                                >  1:802346/1‑149 (MQ=255)
                              gagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaac                                                                                                                >  1:596291/1‑149 (MQ=255)
                              gagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaac                                                                                                                >  1:1167193/1‑149 (MQ=255)
                                    aCCCGCAATTTTTCTTGACCGGGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                          <  2:828598/149‑1 (MQ=255)
                                     cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATAGACGGTGTTATTGACGCCATGCGCGAGCTAAAATAATGGGCTTTGCGCTGGTGGTAGTACCCATCCAg                                                                                                         >  2:1030668/1‑149 (MQ=255)
                                        gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCt                                                                                                      >  2:225828/1‑149 (MQ=255)
                                          aaTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTCGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTgg                                                                                                    <  1:405347/149‑1 (MQ=255)
                                          aaTTTGTCTTGACCGTGACGTCAACATTAATGTCGCTCCCGGCTATGTCCATGAGATCGACAACTTTCAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTgg                                                                                                    <  2:1230806/149‑1 (MQ=255)
                                           aTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGc                                                                                                   >  2:334788/1‑149 (MQ=255)
                                           aTTTTTCGTGCCCGGGATGGCACCCTTAACGGCGATCCCGGCTATGTCCATGAGCACGCCAACTTTGACTTAATCGACGGTGTTATTCACGCCATCCGCGAGCTAACAAAATCGGCAATTCGCTCGAGGTAGTAACCAACCAGTCTGGc                                                                                                   <  2:1181378/149‑1 (MQ=255)
                                              tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCAtt                                                                                                >  2:40418/1‑149 (MQ=255)
                                                   tGACCGTGATTGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGATATTGACGCCATGCGCGAGCTAAAATAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcg                                                                                           <  2:60019/149‑1 (MQ=255)
                                                   tGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcg                                                                                           >  2:734310/1‑149 (MQ=255)
                                                    gACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgc                                                                                          >  2:699850/1‑149 (MQ=255)
                                                     aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGAGCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATTGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgcg                                                                                         <  2:1471490/149‑1 (MQ=255)
                                                       cGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGCCGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGt                                                                                       >  1:1497719/1‑149 (MQ=255)
                                                         tGATGGCCCCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCCGTCTGGCATTTCTCGCGGTaa                                                                                     >  2:390951/1‑149 (MQ=255)
                                                          gATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTaaa                                                                                    <  2:1402344/149‑1 (MQ=255)
                                                          gATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTaaa                                                                                    <  2:1477329/149‑1 (MQ=255)
                                                           aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGCCAACTTTGAATTTATCGACGGTGTTATTGACGACAAGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTACTAACCAACCAGTCTGGCATTGCTCCCGGTaact                                                                                   >  2:1244708/1‑147 (MQ=255)
                                                               cACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTAc                                                                               <  1:24080/149‑1 (MQ=255)
                                                               cACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTAc                                                                               <  1:1459977/149‑1 (MQ=255)
                                                                 ccATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTTCGCTGGTGGTTGTAACCCACCAGTCTGGCATTGCTCGCTGTAAATTTACCg                                                                             >  1:117878/1‑149 (MQ=255)
                                                                   aTTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTTAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTATCCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGaa                                                                           >  1:993524/1‑149 (MQ=255)
                                                                   aTTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGaa                                                                           >  2:1107198/1‑149 (MQ=255)
                                                                      aatggagATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGca                                                                        <  2:300429/143‑1 (MQ=255)
                                                                       aTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGcac                                                                       >  2:511716/1‑149 (MQ=255)
                                                                        tGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGcaca                                                                      <  2:1069636/149‑1 (MQ=255)
                                                                         gTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGAAGCAATGCGCGAGCTAAAAAAATGTGCTTTGAGATGGTGGTAGTAACAAACCAGACTGGCAATGCTAGCGGTAAATATACAGAAGCACAg                                                                     >  2:470264/1‑149 (MQ=255)
                                                                            gATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGttt                                                                  <  1:837890/149‑1 (MQ=255)
                                                                             aTCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTg                                                                 <  1:79631/149‑1 (MQ=255)
                                                                                aCGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGaaa                                                              <  1:341461/149‑1 (MQ=255)
                                                                                  ggCTATGTCCATGAGATCGACAACTTTGAATTTATCGACCGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACg                                                            >  2:1243380/1‑149 (MQ=255)
                                                                                     tATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTGATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTACCCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTg                                                         <  1:719265/149‑1 (MQ=255)
                                                                                        gTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGAcc                                                      >  1:34569/1‑149 (MQ=255)
                                                                                        gTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCAAAAAAAATGGGCTATGCGCTGGTGGTAGTAACCAACCAGTCTGGAATTGCTCGAGGTAAATTAACCGAAGCACACATTGAAACGCTGAcc                                                      >  1:1139953/1‑149 (MQ=255)
                                                                                          ccATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGa                                                    >  1:416410/1‑149 (MQ=255)
                                                                                            aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGt                                                  >  1:414431/1‑149 (MQ=255)
                                                                                            aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGt                                                  >  2:727337/1‑149 (MQ=255)
                                                                                            aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCAGTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGt                                                  >  1:858436/1‑149 (MQ=255)
                                                                                             tGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTCGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtg                                                 >  2:1184432/1‑149 (MQ=255)
                                                                                              gagaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgg                                                >  2:300470/1‑149 (MQ=255)
                                                                                                gaTCGACAACTTTGATTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggat                                              <  1:880105/149‑1 (MQ=255)
                                                                                                 aTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGCGtggatg                                             <  2:1421690/149‑1 (MQ=255)
                                                                                                   cGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgga                                           <  1:781406/149‑1 (MQ=255)
                                                                                                    gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGAc                                          >  2:227131/1‑149 (MQ=255)
                                                                                                     aCAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCTACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                         <  2:209500/149‑1 (MQ=255)
                                                                                                       aaCTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGTCTTTGCTCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTgg                                       <  2:596291/149‑1 (MQ=255)
                                                                                                          tttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAACCGCTGACCGAGTGGATGGACTGGTCg                                    >  2:1462610/1‑149 (MQ=255)
                                                                                                          tttGAATTTATCGACGGGGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTCCGCTGGTGGTAGAAACCAACCAGTCTGGCATTGCACGCGGTAAAATTACCGAACCACAGTAGTAAACCCTGACCAAGTGCATGGACTGGACg                                    >  2:1301732/1‑149 (MQ=255)
                                                                                                           ttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGc                                   <  2:887275/149‑1 (MQ=255)
                                                                                                              aaTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTgg                                <  1:1287872/149‑1 (MQ=255)
                                                                                                                tttATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                              <  1:551259/149‑1 (MQ=255)
                                                                                                                  tATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGa                            <  2:1146839/149‑1 (MQ=255)
                                                                                                                  tATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGGGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGa                            <  2:508015/149‑1 (MQ=255)
                                                                                                                      gACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAAACAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCCAGTGGATGGACTGCTCGCTGGCGGACAga                        >  2:1187233/1‑149 (MQ=255)
                                                                                                                         ggTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCgagct                     >  2:766487/1‑147 (MQ=255)
                                                                                                                         ggTGTTAATGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGGATTGCTCGCGGTAAATTAACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGAt                     <  1:281272/149‑1 (MQ=255)
                                                                                                                           tgtTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGt                   <  1:79035/149‑1 (MQ=255)
                                                                                                                           tgtTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTCGTGGTAGTAACCAACCAGTCTGGCATTGCACGCGCTAAATTTACCCAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGACATGt                   >  1:1086848/1‑149 (MQ=255)
                                                                                                                           tgtTATTGACGCCATGCGAGAGCTAAAAAAATTGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGt                   <  2:1218638/149‑1 (MQ=255)
                                                                                                                               aTTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAt               >  2:267520/1‑149 (MQ=255)
                                                                                                                               aTTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAt               >  1:825580/1‑149 (MQ=255)
                                                                                                                                 tgccGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGAGGGACTGGTCGCTGGCGGACCGAGATGTCGATCt             <  1:1030668/146‑1 (MQ=255)
                                                                                                                                 tGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt             >  2:353308/1‑149 (MQ=255)
                                                                                                                                 tGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt             <  2:802346/149‑1 (MQ=255)
                                                                                                                                 tGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAGCt             <  2:686683/149‑1 (MQ=255)
                                                                                                                                  gACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTg            >  1:749798/1‑149 (MQ=255)
                                                                                                                                   aCGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCTAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTgg           >  2:185400/1‑149 (MQ=255)
                                                                                                                                    cGCCATGCGCGAGCTAAAAACATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGGTTGATACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa          <  2:157854/149‑1 (MQ=255)
                                                                                                                                    cGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa          <  1:300470/149‑1 (MQ=255)
                                                                                                                                    cGCCATGCGCGAGCAAAATACATGGGCTTTGCGCTGGGGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTTAATTTCCCGAAGCACAGTTTGAACCGCGGACCGAGGGGATGGACTGGGCGCTGGCGGACCGAGATCTCGATCTGGa          <  1:63805/149‑1 (MQ=255)
                                                                                                                                       cATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATgg       <  1:334788/149‑1 (MQ=255)
                                                                                                                                          gcgcGAGCTAAAAAAATGGGCTTTGCGCTGGGGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTAt    <  2:1174687/149‑1 (MQ=255)
                                                                                                                                           cgcgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAACCGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATCTCGATCTGGATGGTATc   >  1:1412996/1‑149 (MQ=255)
                                                                                                                                            gcgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCt  <  1:225828/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCT  >  NC_000913/222811‑223100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: