Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,878:1 +G 5.1% intergenic (‑141/+27) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8781.G 5.1% 413.7 / 17.7 99intergenic (‑141/+27)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base . (47/47);  new base G (2/3);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 103 total aligned reads displayed.

CAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAGGCGG  >  NC_000913/2596730‑2597025
                                                                                                                                                     |                                                                                                                                                   
cAACTCATAGATACCTCAACAATATATTCCTTAAATTAAAGAGCGTAGGTGGTGCAGAGGCAGCTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGGTGTCTTGCCGTGCCAGCt                                                                                                                                                      <  1:922793/149‑1 (MQ=255)
cAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCt                                                                                                                                                      >  2:460503/1‑149 (MQ=255)
 aaCGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                                                                    >  2:1468154/1‑149 (MQ=255)
 aaCGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                                                                    >  2:414506/1‑149 (MQ=255)
  aCGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                                                                                   <  2:439987/149‑1 (MQ=255)
   cGCATAGATACCTCAACAATATATTCAATATATAAATCATCGTAGCAGGTTCAGAGGAAGATTACAAAAGTAAAGCGGCATGAAATGGCGGAGGAATGAGATTTAAATGCATATATTCCGAGATCGGTTGACATGCCGTACCAGCTGcc                                                                                                                                                  >  2:759412/1‑148 (MQ=255)
     cATAGATACCTCAACAATATAATCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGACGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                                                                                <  2:416361/149‑1 (MQ=255)
      aTAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACgg                                                                                                                                               <  2:902567/149‑1 (MQ=255)
       tAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                                                                              <  2:1449767/149‑1 (MQ=255)
         gATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCaa                                                                                                                                            >  2:356262/1‑149 (MQ=255)
         caTACCTCAACAATATATTCATTATAGCAATGAACGAAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGATGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                                                                            <  1:1382370/148‑1 (MQ=255)
          aTACCTCAACAAAATATTCAATATAGAAATGATCGTAGGTGCTTCAGCCGAAGATTACCACAGGAAAGCGGGATGAAAAGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGATGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                                                                           <  1:234094/149‑1 (MQ=255)
           tACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTTCAGCTGGCACGGCAAga                                                                                                                                          <  2:702413/149‑1 (MQ=255)
            aCCTCAACAATATATTCATTATAGCAATGATCGTAGGTGGCTCAGAGGAAGATTACCCCAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgat                                                                                                                                         <  1:718688/149‑1 (MQ=255)
            aCCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgat                                                                                                                                         >  2:1133607/1‑149 (MQ=255)
            aCCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCAAGGCAAgat                                                                                                                                         >  2:559187/1‑149 (MQ=255)
              cacaacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGATTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                       >  2:1124835/3‑149 (MQ=255)
              cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTACGATAGAGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                       >  1:998772/1‑149 (MQ=255)
               tctacaaTATATTCATTTTAGAAATGATCGTTTGTCTTTGATAGGAATATTACCAAATTAAATCTGGATTAACTGGCGGATGAATTAGCGTTCAATTCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatgat                                                                                                                                      <  2:1478937/146‑1 (MQ=255)
               tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGTAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCTAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatgat                                                                                                                                      <  2:976570/149‑1 (MQ=255)
               tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatgat                                                                                                                                      >  1:1097265/1‑149 (MQ=255)
               tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatgat                                                                                                                                      >  1:824539/1‑149 (MQ=255)
               tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGCAATGAGCGTTCAATGCATATATTCCAAGAGCGTTAGTCTTACCGTACCACCTGCACCGCAAgatgat                                                                                                                                      >  1:1457824/1‑149 (MQ=255)
                caacaaTATATTCATTATAGAAATGATCGTAGGGGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGatgatg                                                                                                                                     <  2:1520218/149‑1 (MQ=255)
                 aacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCTTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCATCTGCACGGCAAGAtgatga                                                                                                                                    >  2:1395954/1‑149 (MQ=255)
                 aacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCACTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAtgatga                                                                                                                                    >  2:926334/1‑149 (MQ=255)
                    aaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGACAGCTCCACCGCAAGATGATGAACa                                                                                                                                 >  1:998449/1‑149 (MQ=255)
                       atatTCAATATAGAAATGATCGAAGGCGGGTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGACTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaa                                                                                                                              <  2:312434/149‑1 (MQ=255)
                        aatTCATTATAGAACTCATCGAAGTGGGTTCAGAGGAAGAGTACCACAGGAACGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaa                                                                                                                             <  1:1507856/148‑1 (MQ=255)
                          ttCATTTAAGAAATGATCGTCGGTGGTTCAGAGGAAGATTACCACAGTAAAGCGGGAGGATATGGGGGAGGCATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                                                                           <  1:1094835/149‑1 (MQ=255)
                           tCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                                                                          <  1:1468154/149‑1 (MQ=255)
                           tCATTATAGAAATGATCGTAGGTGGTGCAGAGGAACATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                                                                          <  1:754168/149‑1 (MQ=255)
                           tCATAATAGAAATGATCGGAGGTGGTGCAGAGGAAGATTCCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGCTCAATGCATATATACCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                                                                          <  2:720746/149‑1 (MQ=255)
                              ttATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAg                                                                                                                       >  1:962989/1‑149 (MQ=255)
                               tataGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                                                                      >  1:15446/1‑149 (MQ=255)
                                   gAAATGTTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGAAAGATGATGAACAGAAAAATCAGTCCAg                                                                                                                  <  2:1101684/149‑1 (MQ=255)
                                    aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCTTCACGGCAAGATGATGAACAGAAAAATCAGTcccac                                                                                                                 >  1:601149/1‑146 (MQ=255)
                                    aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGc                                                                                                                 >  1:302677/1‑149 (MQ=255)
                                    aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTAGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGc                                                                                                                 >  1:398636/1‑149 (MQ=255)
                                      aTGATCGTCTGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGCAATGAGCTTTCAATGCATATATTCCGTGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCATGATGATGAACAGAAAAATCAGTCctgctg                                                                                                               <  2:1164712/149‑1 (MQ=255)
                                       ttaTCGTAGCTTGTTCAGAGGAATATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCTTTCAATGCATTTATTCCGAGAGCGGTAGGCTTGCCGGGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGGCCAGCTGt                                                                                                              <  1:1267680/147‑1 (MQ=255)
                                       tgctCGTAGCGGGGTCAGACGAAGAGTACCACAGGAATGCGGTATGAAATGGCGGAGCAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCAGGGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGt                                                                                                              <  2:1032917/146‑1 (MQ=255)
                                        gATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                                                                             >  2:1475996/1‑149 (MQ=255)
                                           cGTAGGTGGTTCAGAGGAAGATTACCTCAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTacac                                                                                                          <  1:1480191/149‑1 (MQ=255)
                                             tAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAccc                                                                                                        >  1:1287519/1‑149 (MQ=255)
                                               ggtgttTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGTAGGAATGAGCGTTCAATGAATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                                                                      <  1:1160722/149‑1 (MQ=255)
                                                gtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAgg                                                                                                     >  2:1524244/1‑149 (MQ=255)
                                                 tggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGAATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCTCGTCAAGATGATGAACAGAAAAATCAGTCCAGCTTTACACCCAggc                                                                                                    >  1:657202/1‑149 (MQ=255)
                                                     tCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGgcggcg                                                                                                >  2:656259/1‑149 (MQ=255)
                                                      cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                                                                               <  2:302677/149‑1 (MQ=255)
                                                      cAGAGGAAGATTACCACAGGAAAGAGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                                                                               >  2:69952/1‑149 (MQ=255)
                                                           gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCCGCTGCACGTCAAGATGATGAACAGAAATATCCGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                                                                          >  2:1043722/1‑149 (MQ=255)
                                                           gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                                                                          >  1:658450/1‑149 (MQ=255)
                                                            aaGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTATACACCCAGGCGGCGGGCGACg                                                                                         <  1:356262/149‑1 (MQ=255)
                                                             aGATTAACACAGGAAAGCGGGAGGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTACAGCTGTACCACCAGTCTGCGGGCGCCgg                                                                                        >  2:1051694/1‑149 (MQ=255)
                                                              gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                                                                       <  1:1334034/149‑1 (MQ=255)
                                                              gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                                                                       <  1:1133607/149‑1 (MQ=255)
                                                               aTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                                                                      <  1:254523/149‑1 (MQ=255)
                                                                     acaGGAACGCGGGATCAAATGGCGTAGGCATGAGCGTTCAATGCATATATACCGAGACCGGTTGTCTTGCCCTGCCAGCAGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCGGTACACCCAGGCGGCGGGCGACGGCTTCATAg                                                                                <  2:1174137/149‑1 (MQ=255)
                                                                          acaGCGGGATGAAAGGGCGGAGGAATGAGCGTTCAATGCATATATTCCTCGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                                                                           <  2:536006/147‑1 (MQ=255)
                                                                                ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATAATCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg                                                                     <  2:633810/149‑1 (MQ=255)
                                                                                     aaaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGGAGTACACACAGGCGGCGGGCGACGGCTTCATAGGCTACGATCAGGCCAc                                                                >  2:1151473/1‑149 (MQ=255)
                                                                                       aTGGCGGAGGAATGCGCGTGCAATGCATATCTTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAGCATGCCAGCTGTACACCCAGTCGCCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg                                                              <  2:1147485/149‑1 (MQ=255)
                                                                                        tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGa                                                             >  1:25955/1‑149 (MQ=255)
                                                                                        tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATAAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCATGCCACCGa                                                             >  1:925273/1‑149 (MQ=255)
                                                                                        tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGATGTCTAGCCGTCCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTACGATCAGGCCACCGa                                                             >  1:977132/1‑149 (MQ=255)
                                                                                         ggCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTGCGATCAGGCCACCGAg                                                            <  1:1314458/149‑1 (MQ=255)
                                                                                               ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTGGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTg                                                      <  2:240826/149‑1 (MQ=255)
                                                                                                  aTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg                                                   >  1:824226/1‑149 (MQ=255)
                                                                                                    gAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCTCGGCAAGATGATGATCAGAAAAATAAGTCGAGCTGTACACCGCGGCGGCGGGCGACGGCTTCATAGGCTTCGCTCAGGCCCCCCAGGCTCTGGCGGa                                                 >  2:1199590/1‑149 (MQ=255)
                                                                                                      gCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCCCGGCAAGATGATGAACAGAAAAATCAGTACAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGaaa                                               >  1:809173/1‑149 (MQ=255)
                                                                                                          tCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTGCGATCCGGCCACGGAGGCTCTGGCGGAAACGGt                                           >  1:1099595/1‑149 (MQ=255)
                                                                                                              tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt                                       <  1:656259/149‑1 (MQ=255)
                                                                                                                  tatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCAGGCGGAAACGGTCTTTGTcc                                   >  1:1264712/1‑149 (MQ=255)
                                                                                                                      ttCCGATAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAttt                               <  2:601149/149‑1 (MQ=255)
                                                                                                                      ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCCGGCCCCCGAGGCTCTGGCGGAAACGGGCGTTGTCCAttt                               >  1:950258/1‑149 (MQ=255)
                                                                                                                         cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGGTTCATAGGCTTCGATCCGGCCACCGAGGCTCTGGCGGAATCGGGCTTTGTCCAGTTtct                            >  1:719206/1‑149 (MQ=255)
                                                                                                                          gagaGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAACTCAGTCCAGCTGTACACCCCGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCCCCGAGGCTCTGGCGGAGACGGTCTTTGTCCATTTtctc                           >  2:708714/1‑149 (MQ=255)
                                                                                                                                ggTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGt                     >  1:260814/1‑149 (MQ=255)
                                                                                                                                     tCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGTCGGCGCGCGACGGCTTCAGAGGATTCGATCATGCCACCGAGGCTCTGGCGGAAACGGTCTTTCTCCATTTTCTCCAGCGTTTCtt                >  2:335663/1‑149 (MQ=255)
                                                                                                                                      gttggagTGCCAGCTGCACGGCATGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGTCGACGGCTTCATAGGCTTAGATCAGGCCACAGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGAGTTTCttt               <  2:1178068/143‑1 (MQ=255)
                                                                                                                                      cTTGCCGTGCCAGTTGCACGGGAAGATGATGAACAGAAAAATCAGTCCCGCTGTACACCCGGGCGGGGGGCGACGGCTTCATAGTCGTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt               <  2:1217394/149‑1 (MQ=255)
                                                                                                                                       ttGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTg              >  2:148831/1‑149 (MQ=255)
                                                                                                                                       ttGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTg              >  1:346224/1‑149 (MQ=255)
                                                                                                                                        tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt             <  1:180560/149‑1 (MQ=255)
                                                                                                                                         gCCGTGCCAGCTGCTCGGCAAGATGATGCACAGCTATATCAGGCCCGCTGTACACGCAGGCGGCGGGCGCCGGCTTCATAGGCTTCGTTCTGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTGCATTTTCTCCAGCGTTTCTTTGTc            <  1:254485/149‑1 (MQ=255)
                                                                                                                                         gCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCTGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc            <  1:926334/149‑1 (MQ=255)
                                                                                                                                         gCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc            <  1:1124835/149‑1 (MQ=255)
                                                                                                                                          gccgagcAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCCCCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc            >  2:1082092/7‑149 (MQ=255)
                                                                                                                                          ccGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTCCACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTcc           <  1:1395954/149‑1 (MQ=255)
                                                                                                                                           cGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTCCACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTGTCTTTGTccc          >  1:1044826/1‑149 (MQ=255)
                                                                                                                                           cGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGTCGGCGGGCGACGGCTTCCTAGGCTTCGATAAGGCCACCGAGGCTCTGGCGGAAAGGGTCTTTGTCCATTTTCTCCAGCGTATCTTTGTccc          >  1:253467/1‑149 (MQ=255)
                                                                                                                                           cGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTccc          <  2:658450/149‑1 (MQ=255)
                                                                                                                                            gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTGCATAGGCTTCGATCAGGCCCCCGAGGCTCTGGGGGAAACGGTCTTTGTCCAATTTCTCGAGCGTTTCTTTGTCCca         >  1:760324/1‑149 (MQ=255)
                                                                                                                                             tGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCcac        <  1:1524244/149‑1 (MQ=255)
                                                                                                                                              gCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCcaca       <  1:612205/149‑1 (MQ=255)
                                                                                                                                               ccAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAg      >  2:1231255/1‑149 (MQ=255)
                                                                                                                                                 aGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGCTCAGGCCCCCGAGGCTCTGGCGGACACGGGCTGTGTCCATATTCTCCAGCGGTTCTTTGTCCCACAggc    >  2:320778/1‑149 (MQ=255)
                                                                                                                                                   cTGGACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGAGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAggcgg  <  1:1311257/149‑1 (MQ=255)
                                                                                                                                                   cTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAggcgg  <  2:962989/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAGGCGG  >  NC_000913/2596730‑2597025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: