Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,158:1 +A 5.9% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1581.A 5.9% 397.8 / 20.9 101intergenic (+23/+20)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (39/56);  new base A (2/4);  total (41/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

Only 100 of 102 total aligned reads displayed.

GCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAC  >  NC_000913/120013‑120300
                                                                                                                                                  |                                                                                                                                              
gcgcGTGAACCGCATGTCGATAAGGGGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGGCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                                                                             <  2:1519909/149‑1 (MQ=255)
    gTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCATTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                                                                         <  1:585715/149‑1 (MQ=255)
     tGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGGGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                                                                        <  1:249433/149‑1 (MQ=255)
     ggAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGGGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                                                                        <  1:416083/148‑1 (MQ=255)
     ggAACCGCAGGTCGATAAGCGGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAgg                                                                                                                                        <  2:1278349/148‑1 (MQ=255)
      gAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                                                                       <  2:213174/149‑1 (MQ=255)
        aCCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGGGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCg                                                                                                                                     <  2:812773/149‑1 (MQ=255)
        aCCGAATGTCGATAAGGGGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTGCGGGGGCGTGGTGGTGAATGCACTACTGACGATATACGGGGCGTTGGTGTAAAGATTATTGCCCTCCCCCGTACAGGGTGAGGGca                                                                                                                                     <  1:1057697/149‑2 (MQ=255)
         ccGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGt                                                                                                                                    >  2:497364/1‑149 (MQ=255)
         ccGCATGTCGAGAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGATCGTGGTCGTGGTGGAGAGTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGt                                                                                                                                    <  1:55358/149‑1 (MQ=255)
          cGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTa                                                                                                                                   <  1:1448843/149‑1 (MQ=255)
          cGCATGTCGATAACGGGGAGCCGCCGAAGGCATCGGTTTCAATGGCGAAGAAATCCTCGTTCGTGCTCGTGGTGGTGTTTGCATTAGTGACGATTTACGGGTCGTTTGTTTAAAGATTATTGCCGTCTCCCTGTACGGGTTATGGCGTa                                                                                                                                   <  2:298554/149‑1 (MQ=255)
           gCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                                                                  >  2:419517/1‑149 (MQ=255)
              tGTCGCTCAGGGGGAGCCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGCTCGTGGTCGTGCTGGTGATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                                                               <  1:1472839/149‑1 (MQ=255)
               gTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                                                                              <  2:822236/149‑1 (MQ=255)
               gTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                                                                              <  2:1228501/149‑1 (MQ=255)
               gTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGGGGTGGAGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                                                                              >  2:1196558/1‑149 (MQ=255)
                 cGATCATGTGGAGACGCCGAAGGCAGCGGGTTCAATGGCGAAGAATACCTCGTTCGTGGTCGTGGTTGTGCTTGCACTACTGACGATTTACGGGGCGTTGTTGTAAAGATTATTGCCCTCACCCTGTACGGGTGATGGCGTAGAGAGAt                                                                                                                            <  1:758085/149‑1 (MQ=255)
                   aaaaGGTGGAGACGCCGAAGCCAGCGGTTTCAATGGCGAAGAAAACCTCGGTCGTGGGCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                                                                          <  2:724960/147‑1 (MQ=255)
                   aTAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                                                                          >  1:651523/1‑149 (MQ=255)
                   aTAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                                                                          >  1:176302/1‑149 (MQ=255)
                   aTAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                                                                          >  1:912169/1‑149 (MQ=255)
                     acgggggAGACGCCGAAGGCAGCGGGTTGACTGGCGACGAAACCCTCGTTCGTGGTCTTGGTGGGGATTGCCCTACTGACGATTTCCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                                                                        <  1:958091/144‑1 (MQ=255)
                       ggTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                                                                      >  1:439617/1‑149 (MQ=255)
                           gagaCGCCGAAGGCAGCGGTTTGACTGGCGAAGATAACCTCGTTCGGGGTCGGGGTTGTGATTGCACTACTGACGAATTACGGGGCGTTGGTGGTAAGATTATTGCCCTGCCCCTGTCCGGGTGAGGGCGTAGAGTGAGTAATGCGCtt                                                                                                                  <  2:1459617/149‑1 (MQ=255)
                           gagaCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTATTGCGCtt                                                                                                                  >  2:1517449/1‑149 (MQ=255)
                                gCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACg                                                                                                             <  1:50214/149‑1 (MQ=255)
                                 ccGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACgg                                                                                                            <  1:1041098/149‑1 (MQ=255)
                                        cAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                                                                     >  1:660440/1‑149 (MQ=255)
                                        cAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                                                                     <  1:1445103/149‑1 (MQ=255)
                                         aGCGGTTTCAATGGCGAAGAAAACATCGTTCGAGGTCGTGGTCGTGATTGCACTACTGACGATTTACGGGGCTTTGGTGTAAAGATTATTGCCCTCACCCTGTACTGGTGAGGGCCTAGAGAGAATAATGCGCTTTTTCGGCTTAGGCg                                                                                                    >  1:1415087/1‑149 (MQ=255)
                                           cGGTTTCAATGGCGAAGAAAACCTCGTTAGTGGTCGTGGTGGTGATTGAACTACTGACGATTTACGTGGCGTTGGTGTAAAGATTATTTCCCTCACCCTGTACGCGTGAGGGCGTAGAGAGATTAATGAGCTTTAACGGCTTTGGCGGt                                                                                                  >  1:1441414/1‑149 (MQ=255)
                                            ggTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                                                                 <  1:1555108/149‑1 (MQ=255)
                                            ggTTTCAAAGGCGAAGAAAACCTCGTTCGTGGTCGTTGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                                                                 <  1:1537804/149‑1 (MQ=255)
                                              tttCAATGGCGAAGAAAACCTCGTACGTGGACGTGGAGGTGATTGCACTACTGACGCATAACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGATAGATTAATGCGCTTTAACGGCTTTGGAGGtgaa                                                                                               >  1:220089/1‑146 (MQ=255)
                                                tCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                                                                             <  1:420081/149‑1 (MQ=255)
                                                 cAAAGGCGAAGAAAACCTCGTGCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                                                            <  2:126788/149‑1 (MQ=255)
                                                  actgagcaagaaACACTAGTTGGTTGTAGTGGTGGTGATTTCAATTCGCACGATTTTCGGGGCGTTGGGGTAAAGCTTATTGCCCTCACCCTTTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGAGGTTTTctct                                                                                           <  2:1441414/142‑1 (MQ=255)
                                                   aTGGCGAAGACAACCTCGAGCGTGGGCGTGGGGGTGATTGCACTTCGGCCGATGTACGGGGCGTTTGGGTAAAGAATATTGCCCTCAACCTCTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCtt                                                                                          <  2:1438430/149‑1 (MQ=255)
                                                   aTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCtt                                                                                          >  2:1515325/1‑149 (MQ=255)
                                                   aTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCtt                                                                                          <  2:596303/149‑1 (MQ=255)
                                                    tGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACCGACGATTTACGGTGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt                                                                                         <  2:213495/149‑1 (MQ=255)
                                                     ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                                                                        <  1:116357/149‑1 (MQ=255)
                                                     ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATAATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                                                                        <  2:323584/149‑1 (MQ=255)
                                                       ggtagaaAACCGCGGTCGTGGTCGGGGTGGTGATTGCACAACTGTCGATTTCCGGGGCGTTGGTGTATAGATTATTGCCCTCACCCTGTCCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                      <  2:1068185/146‑1 (MQ=255)
                                                       cgcagaaAACCTCGTTCGTGGTCGTGGTGGTGTTTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCGCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTCGCGGTTTTCTCTTTaaa                                                                                      <  2:1339728/146‑1 (MQ=255)
                                                       cGAAGAAAACCGCGTTCGTGGTCGTGGTGGTGAATGCACTACTCACGATTAACGGGGCGTTGATGTAAACATTATTGCCCTCTCCAAGTACGGGTGAGGGCCTAGAGAGATTAATGCGATATAAAGGCTTTGGCGGTTTTCTCTTTaaa                                                                                      >  1:162057/1‑149 (MQ=255)
                                                       cGAAGAAAACCGCGTTCGTGGGCGTGGTGGTGATTGCACTACTGACGATTTACGGTGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                      <  2:555420/149‑1 (MQ=255)
                                                        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                                                                     <  2:514062/149‑1 (MQ=255)
                                                        gaagaaAACCTCGTGCGTGGGCGGGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                                                                     <  1:1323847/149‑1 (MQ=255)
                                                               aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                                                              <  1:35682/149‑1 (MQ=255)
                                                               aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                                                              <  2:978190/149‑1 (MQ=255)
                                                               aCCTCGTTCGGGGGCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTAATGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                                                              <  2:1469953/149‑1 (MQ=255)
                                                                 cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAATGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTATTACGGCTTTGGCGGTTTTCTCTTTAAACATATAGCCg                                                                            >  2:317672/1‑149 (MQ=255)
                                                                  tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                                                                           <  2:482249/149‑1 (MQ=255)
                                                                    gttcggGGGCGTGGTGGCGATTGCACTACTGACGATTTCCGGGGCGGGGGGGTAAAGAGTATTGCCCGCACCCTGGACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa                                                                         <  1:931685/149‑1 (MQ=255)
                                                                         tggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGATGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                                                                    >  1:398219/1‑149 (MQ=255)
                                                                           gtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca                                                                  >  2:317513/1‑149 (MQ=255)
                                                                           gtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTCGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGCTAGCCGATACCTAacc                                                                  >  2:291638/1‑148 (MQ=255)
                                                                                ggtggtgATTGCACTACTGATGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATc                                                             >  1:996536/1‑149 (MQ=255)
                                                                                 ggggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa                                                            <  2:1402639/147‑1 (MQ=255)
                                                                                   tgtgATTGCACTAATGACGTTTTACGGGGCGTTTGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGAGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc                                                          <  2:55134/148‑1 (MQ=255)
                                                                                    gtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc                                                         >  1:1526888/1‑149 (MQ=255)
                                                                                      gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCAt                                                       >  2:607695/1‑149 (MQ=255)
                                                                                           cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCATCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACAATCAGCCATACCgg                                                  >  2:1278316/1‑149 (MQ=255)
                                                                                             ctactGACGAATTACGCGGCGGTGGTGTATAGATTATTGCCCTCCCCATGTTCGGGTGAGTGCGTAGATAGCTTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGa                                                <  1:317672/149‑1 (MQ=255)
                                                                                              tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGAt                                               >  2:198797/1‑149 (MQ=255)
                                                                                                 tGACGATTTACGGGGCGTTGGTGTACAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            <  1:919625/149‑1 (MQ=255)
                                                                                                 tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            <  2:461195/149‑1 (MQ=255)
                                                                                                 tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            <  1:1125789/149‑1 (MQ=255)
                                                                                                 tGACGATATCCGCGGCGTTGGTCTAAAGAATATTGCCCTCACCCTCTACGGGCGAGGGCGTAGAGACATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCATTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                            <  2:685254/149‑1 (MQ=255)
                                                                                                  gACGATGTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTATTTATACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAgg                                           <  1:1193897/149‑1 (MQ=255)
                                                                                                   aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGt                                          >  1:521606/1‑149 (MQ=255)
                                                                                                       tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAc                                      <  1:1311407/149‑1 (MQ=255)
                                                                                                        ttACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAcc                                     <  2:398219/149‑1 (MQ=255)
                                                                                                            ggggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGaaa                                 >  2:982007/1‑149 (MQ=255)
                                                                                                             gggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAAt                                <  2:815382/149‑1 (MQ=255)
                                                                                                              gtcGTTGGTGTAAAGATTATTGCCATCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATTCGCTTTTACGGCTTTTGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCCGCCATACCGGGATCAGGTATACCGAAATc                               <  2:88253/147‑1 (MQ=255)
                                                                                                                     gtgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAAGCGCTTTTCCGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGAAAACCGACATCGCCAGTc                        >  2:850878/1‑149 (MQ=255)
                                                                                                                      tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGAGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTcc                       >  2:1499149/1‑149 (MQ=255)
                                                                                                                       gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGTCGGTTTTCTCTTTAAACAGATAGCCGCTACCTAACACGATCAGCCATACCGGGATCAGGTATCACGAAATCGCCATTCCt                      >  2:339723/1‑149 (MQ=255)
                                                                                                                        tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACCGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATAAGCCATACCGGGATCAGGTATACCGAAATCGACAATCCTg                     >  2:1094910/1‑149 (MQ=255)
                                                                                                                         aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTgg                    >  1:387025/1‑149 (MQ=255)
                                                                                                                         aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTgg                    >  1:308471/1‑149 (MQ=255)
                                                                                                                          aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                   >  2:245484/1‑149 (MQ=255)
                                                                                                                          aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                   <  2:912169/149‑1 (MQ=255)
                                                                                                                          aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTGCGCTTTATACAGATTGCCGATACCTAACACGATCAGCCATAACGGGATCAGGTATACGGAAATCGCCATTCCTggg                   >  1:886350/1‑149 (MQ=255)
                                                                                                                          aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGATTTTACGGCTTTGGCGTTTTTATCTTTAAACAGATAGCCGATACATAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                   <  2:651523/149‑1 (MQ=255)
                                                                                                                          aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATAAATGCGCTTTTACGGCTATGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                   >  1:425358/1‑149 (MQ=255)
                                                                                                                                  tGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAg           <  2:1552029/149‑1 (MQ=255)
                                                                                                                                   gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGc          <  1:811388/149‑1 (MQ=255)
                                                                                                                                   gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCGTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCAATTCCTGGGGGCATCAGc          >  1:1183162/1‑149 (MQ=255)
                                                                                                                                   gCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACCCGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGc          >  1:123641/1‑149 (MQ=255)
                                                                                                                                     ccTCACCCTGTACGCGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGAAAGCCGATACCTAACACGATCAGCCATACCGGCATCAGGTATACCGAAATCCCCATGCCTGTGGAAATCAGCAt        >  2:153503/1‑149 (MQ=255)
                                                                                                                                     ccTCACCCGTACAGGGTGAGGGCGTAGCGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATTCCGGGATCAGGTATACCGAAATCGCCATTACTGGGGTCTTCAGCAt        >  2:1097579/1‑149 (MQ=255)
                                                                                                                                      cTCACCCTGTCCGGGTGAGGGCGAAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATACCTGGGGTCATCAGCATa       <  2:162169/149‑1 (MQ=255)
                                                                                                                                      cTCAACCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTTAACAGAAAGCCGATACGTTACACGATCAGCGATGCCGGGAGGAGGTATAGCGAAATCTCCATTACTGGGGGCATCAGCATa       >  2:1022399/1‑149 (MQ=255)
                                                                                                                                        cACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAAt     <  1:1196558/149‑1 (MQ=255)
                                                                                                                                           ccTGTCCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAc  <  1:1364631/149‑1 (MQ=255)
                                                                                                                                           ccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTATACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAc  <  2:184715/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAC  >  NC_000913/120013‑120300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: