| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 87,793 | A→C | 13.8% | E146A (GAG→GCG) | ilvH → | acetolactate synthase 3, small subunit, valine‑sensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 87,793 | 0 | A | C | 13.8% | 229.9 / 17.0 | 103 | E146A (GAG→GCG) | ilvH | acetolactate synthase 3, small subunit, valine‑sensitive |
| Reads supporting (aligned to +/- strand): ref base A (52/36); new base C (7/8); total (59/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GGGCGTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCA > NC_000913/87645‑87941 | gggCGTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCTCCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGa < 2:785742/149‑1 (MQ=255)gggCGTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGa < 2:1547232/149‑1 (MQ=255) gTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCCCACCCTCGCATTATACCGTTCAATTAGCAGGCACCAGCGGGAAGCTAGATGCATTTTTAGCATCGATTCGCGAGGAGGCGAAAATTGTGGCGGtt > 2:1048504/1‑149 (MQ=255) gTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTAGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGtt > 1:159145/1‑149 (MQ=255) gTGACGAAGTGAAACGTAATACGGAAATAATCCGTGGGCAAATTATCGAAGTCACACCCTCGCTTTATACCGTTCAATGAGCAGGCACCAGCGGTAAGCTTGATGCATGTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGtt > 2:1044481/1‑149 (MQ=255) gACGAAGTGAAACGTAATACGGAAATATACCTTGTGCAAATTATCGATGTCACACCCTCGCTTTTTACAGTTCAATTAGCAGTCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTgc < 2:1483933/149‑1 (MQ=255) gTGAAACGAAATACGGAAATATTCCGTGGGCAACTAATCGATGTCACACCCTCGCTATCTACCGTTCATTTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTACCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTgctcgctc < 2:707189/149‑1 (MQ=255) gAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTATATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCAGTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTg > 1:388532/1‑149 (MQ=255) aaaCGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTgg < 1:395050/149‑1 (MQ=255) aaCGTAATACGGAAATATACCGTGGGCAAATTCTCGAAGGCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCAATTTTAGCATCGATTCGCGATGTGGCGAAAATAGTGGAGGTTGCTCGCTCTGgt < 2:938782/149‑1 (MQ=255) aCGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGATAATTGTGGAGGTTGCTCGCTCTGgtg > 1:108781/1‑149 (MQ=255) aCGGAAATATTCCGTGGGCAAATTATCGATGTCCCACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCgg > 2:277285/1‑149 (MQ=255) ggAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGAc > 2:9605/1‑149 (MQ=255) ttCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTcgcg > 2:34780/1‑149 (MQ=255) cGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCTATTCGCGATGTGGCGAAAATTGTGGAGGGTGCTCGCTCTGGGGTGGTCGGACTTTCGCgcgg > 2:701445/1‑149 (MQ=255) cGTGGGCAAATTATCGATGTCACACCCTCGATTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCAGTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCgcgg < 1:165427/149‑1 (MQ=255) gTGGGCAAATTATCGATGTCACACCCTCGCTTTAAACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCgcggc < 2:1319499/149‑1 (MQ=255) gTGGGCAAATTATCGATGTCACACACTCGCTTTATACCGTTCAAAAAGCAGGCACCAGAGGTAAGCTTGATGCAATTTTAGCATCGATTCGCGATGTGGAGAAAATTATGGAGGTTGATCACTATGGAGTGGTCTGACTTTCTCgcggc > 2:1145674/1‑149 (MQ=255) gggCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCTTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGa > 2:1130323/1‑149 (MQ=255) gggCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGa > 1:638104/1‑149 (MQ=255) ggCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGAt > 1:774706/1‑149 (MQ=255) ggCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGAt > 1:545867/1‑149 (MQ=255) gCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGTTGTGGTCGGACTTTCGCGCGGCGATa > 1:96337/1‑149 (MQ=255) gCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATa > 1:1389746/1‑149 (MQ=255) gCAAATTATCGATGTCACACCCACGCTATATACAGTTCAATTAGCAGGCACCAGCGGAAAGCTTGATGAATATTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTCTTCGGGCTTTCGCGCGTCGATa > 1:143484/1‑149 (MQ=255) tATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAaataa < 1:549060/149‑1 (MQ=255) tCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATg > 2:1407201/1‑149 (MQ=255) gATGTCCCACCCTCGCGTTATACCTTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCAGTTTTAGCATCGATTCTCGATGTTACGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCg < 1:301217/149‑1 (MQ=255) gATGTCACACCCTCGCTTTATACCGTTCTATTCGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGGTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCg < 1:1130108/149‑1 (MQ=255) gATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTGGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCg < 2:1494057/149‑1 (MQ=255) aTGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGAGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGt < 1:456628/149‑1 (MQ=255) gTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTg > 1:1537485/1‑149 (MQ=255) tCACACCCTCGCTTTATTCCGTTCAATTCGCAGGCACCAGCGGTAAGCTTGATGCTTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTATCGCGCGGCGATAAAATAATGCGTTga < 1:937760/149‑1 (MQ=255) tCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTga < 1:347720/149‑1 (MQ=255) tCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTga > 1:1234445/1‑149 (MQ=255) tCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTga > 2:4722/1‑149 (MQ=255) cacCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGAGGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGaa < 2:790953/149‑1 (MQ=255) acCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAAt > 2:618709/1‑149 (MQ=255) ccTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTGTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGa > 1:401411/1‑149 (MQ=255) ccTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATATAATAATGCGTTGAGAATGa > 2:163057/1‑149 (MQ=255) cTCGCTTTATACCGTTCACTTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCTGCGATAAAATAATGCGTTGAGAATGAt < 1:498810/149‑1 (MQ=255) tCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGAtc < 1:62588/149‑1 (MQ=255) cGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTTGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGAtct < 2:1269590/149‑1 (MQ=255) cGCTTTATACCGTTCAATTAGCAGGCACCAGCGGAAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGAtct < 2:802220/149‑1 (MQ=255) gCTTTTTACCGTTCATTTAGCAGGAATCTTCGGTAAGCTTGATGAATTTTTAGGATGGTTTCGAGATGTGGCGAAATTTGGGGAGGTTGCTCGCTCTGGTGGGGTCGGAGGTTCGCGCTGCGATAAAATAATGCGTTGAGAATGAtctc < 2:1076918/149‑1 (MQ=255) tttATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGATAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCaa < 2:723591/149‑1 (MQ=255) tttATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATATAATAATGCGTTGAGAATGATCTCaa > 1:935278/1‑149 (MQ=255) cGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCaa > 2:56356/1‑149 (MQ=255) cGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCaa > 2:201426/1‑149 (MQ=255) gTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAt > 2:1266942/1‑149 (MQ=255) gTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAt > 2:369998/1‑149 (MQ=255) ttCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAtt < 1:1369291/149‑1 (MQ=255) ttCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAtt > 1:1424586/1‑149 (MQ=255) ttCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAtt > 1:37769/1‑149 (MQ=255) ttCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGAGTTGAGAATGATCTCAATGCGCAAtt > 1:206518/1‑149 (MQ=255) tCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAttt < 1:1336813/149‑1 (MQ=255) aaTTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTAc > 1:831142/1‑149 (MQ=255) aTTAGCAGGCTCCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCTATGTGGCGAATATTGTGGAGGTTGCTCGCTCTGGTGTGGTCTGACTTTCGCGCGGCGATAAAATAATGCTTTGAGAATGATCTCAATGCGCAATTTACa < 2:43026/149‑1 (MQ=255) acAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCAATTCGCGATGTGGCGAAAATTGTGGAGGTAGCTCGCTCTGCTGTGGTCGGACTATCGCGAGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACATcca > 1:923346/2‑148 (MQ=255) ggCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAAc < 2:220988/149‑1 (MQ=255) gCAACAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCTGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACa > 2:742073/1‑149 (MQ=255) cACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACAt > 1:1282993/1‑149 (MQ=255) cAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTTGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGGTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTc < 1:1048504/149‑1 (MQ=255) cAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTGGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTc < 1:1044481/149‑1 (MQ=255) cGGTAAGCTTGATGCATTTTTTGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGAGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg < 1:391860/149‑1 (MQ=255) aGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGAGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTggg < 1:1275143/149‑1 (MQ=255) gcatgattATCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTTCTCGCTCTGGTGTGGTCGGACTTTCGGGCGGCGATAAAATATTGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttttt < 2:866551/143‑1 (MQ=255) gCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttttt < 1:277285/149‑1 (MQ=255) aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGCGGATGCGCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGc < 2:720136/149‑1 (MQ=255) aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGc < 1:833786/149‑1 (MQ=255) aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGAGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGCTTTTTTTGc > 2:741278/1‑149 (MQ=255) tttttAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg > 1:513374/1‑149 (MQ=255) cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg > 2:1127067/1‑149 (MQ=255) aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt < 2:1424586/149‑1 (MQ=255) aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt < 1:231133/149‑1 (MQ=255) aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt > 1:765921/1‑149 (MQ=255) tCGAATCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg < 2:1199531/149‑1 (MQ=255) aTTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGACATCAGTGTGa > 1:628191/1‑149 (MQ=255) tCGCGATGTGGCGAAAATTGTGGCGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAc < 2:545867/149‑1 (MQ=255) cgATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTg < 2:1210606/149‑1 (MQ=255) tgtgGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa < 1:34780/149‑1 (MQ=255) tgtgGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa > 1:549711/1‑149 (MQ=255) tgtgGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa > 2:792103/1‑149 (MQ=255) tgGCGAAAATTGTGGAGGTTGCTCGCTCCGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTCGAAATCAGTGGGAACCTGGAATa > 2:1048075/1‑149 (MQ=255) gCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaa > 1:1097755/1‑149 (MQ=255) cGAAAATTGTGGAGGTTGCTCGCTCTGGGGTGGTCGGACTTTCGCGGGGCGATAAAATAATGCGTTGCGAAGGATCTCAATGCGCAATTTACAGCCCAACATGTCCCGTTGGGCTTTTTTTGCGAAATCACTGGGAACCTGGAATaaaa < 2:487308/149‑1 (MQ=255) gAAAATTGTGGCGGTTGATCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTTGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg < 1:879086/149‑1 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGTACCTGGAATAAAAg > 1:266327/1‑149 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaaac > 2:937322/1‑148 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg > 1:257070/1‑149 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCAAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg > 1:205579/1‑149 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCCATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAGTTACAGCCCACCATGTCCCGAGGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg > 1:853661/1‑149 (MQ=255) gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTATCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATATACAGCCCAACATGTCACGTTGTTCTTTTTTTTCGAAATAAGTGGGAACCTGTAATaaaac > 2:1094135/1‑148 (MQ=255) aaaaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCAAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc > 2:678443/1‑149 (MQ=255) aaaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCa < 2:159145/149‑1 (MQ=255) aaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGCATAAAAGCAg > 2:1558943/1‑149 (MQ=255) aaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg > 1:426551/1‑149 (MQ=255) ggAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCg > 2:731950/1‑149 (MQ=255) gAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAATATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCCACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc > 1:980990/1‑149 (MQ=255) aGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa < 1:201426/149‑1 (MQ=255) | GGGCGTGACGAAGTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTATACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCA > NC_000913/87645‑87941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |