Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,691,171 T→A 5.9% noncoding (170/184 nt) glmY ← sRNA activator of glmS mRNA, glmZ processing antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,1710TA 5.9% 295.1 / 5.2 108noncoding (170/184 nt)glmYsRNA activator of glmS mRNA, glmZ processing antagonist
Reads supporting (aligned to +/- strand):  ref base T (51/50);  new base A (4/3);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 108 total aligned reads displayed.

GCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGG  >  NC_000913/2691029‑2691313
                                                                                                                                              |                                                                                                                                              
gcagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCg                                                                                                                                          <  1:944658/149‑1 (MQ=255)
  agAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGc                                                                                                                                        <  1:1444799/149‑1 (MQ=255)
     aTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCttt                                                                                                                                     <  1:613610/149‑1 (MQ=255)
     aTCAGCAAAAAAGCCAGCTTTACCAGTTGTCGTAATGAGCGTGGACAAACGGGCCAGCGGTTCAATGTTTTACTCTCGTCAGACTCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCttt                                                                                                                                     <  2:1394227/149‑1 (MQ=255)
         gCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCGGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTCCGCGGCTTTGTTa                                                                                                                                 >  2:853773/1‑149 (MQ=255)
          cAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTAt                                                                                                                                >  2:835060/1‑149 (MQ=255)
            aaatTGCGAGCATTACCAGTTGTAGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATgg                                                                                                                              <  1:157201/149‑1 (MQ=255)
            aaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATgg                                                                                                                              <  2:818895/149‑1 (MQ=255)
             aaaTGCAAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCAGGGAATTCCCCGGCTTTGTTATGGa                                                                                                                             >  1:1021576/1‑149 (MQ=255)
                tGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGGGTTACTCTCGGCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATa                                                                                                                          <  2:1224265/149‑1 (MQ=255)
                 gCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTAACTCTCGTCAGACGCGAATAGCCTGATGCTAACCCAGGGGAAGTACAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATaa                                                                                                                         >  1:335130/1‑149 (MQ=255)
                   cAGCATTACCAGTTGTCGTAATGTGCGGGGAAAAACGGGCCAGCGTTTCAAGGGGTTTCTCTCGTCAGACGCGAATAGCCTGATGCTAACCGATGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAgg                                                                                                                       <  2:1137351/149‑1 (MQ=255)
                   cAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGGGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAgg                                                                                                                       <  2:1392392/149‑1 (MQ=255)
                      cATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCgg                                                                                                                    >  1:806803/1‑149 (MQ=255)
                      cATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTAGTTATGGAATAAGGCgg                                                                                                                    >  1:800206/1‑149 (MQ=255)
                       aTTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGt                                                                                                                   <  2:1524242/149‑1 (MQ=255)
                         tatcAGTTGTCGTAATCAGCGGGGAAAAACGGGCCAGCTTTTCAAGGTGTTACTCTCTTCAGACGAGAATAGACTGATGCTAACCGAGGGGAAGTTCAGATACAACTAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                 <  2:1157788/146‑1 (MQ=255)
                         tACCGGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                 <  1:517164/149‑1 (MQ=255)
                            cAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTa                                                                                                              >  1:1488472/1‑149 (MQ=255)
                                   cGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAc                                                                                                       <  1:1297441/149‑1 (MQ=255)
                                    gTAATGAGCGGGGAAAACCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACg                                                                                                      <  2:1251720/149‑1 (MQ=255)
                                    gTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACg                                                                                                      >  1:568378/1‑149 (MQ=255)
                                     tAATGAGCGGGTTAATACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGTATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGt                                                                                                     <  1:965372/149‑1 (MQ=255)
                                     tAATCATCGTGGAAAAACCGGGCTTGGTTTCAAGGTGTTACTCTCGTCAGACGCGATTAGCCTGATGCTAACCGAGGGGTAGTTCAGATTCAACAACGCCGGGAATTACCCGGCTTTGTTATGGAATATGGCGGTGCCTAACTGGACGt                                                                                                     <  2:1407527/149‑1 (MQ=255)
                                       aTGAGCGGGGAAAAACGGGCAAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGATCAGATACAACAAAGCCGGGAATTACGCGGCTTTGTTATGGAATAAGGCGCTGCCTAACTCGACGttt                                                                                                   >  1:1243348/1‑149 (MQ=255)
                                          tgaggggAAAAACGGGCCAGAGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAATCCGGCAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGc                                                                                                <  1:1017532/146‑1 (MQ=255)
                                          aGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGc                                                                                                <  1:695344/149‑1 (MQ=255)
                                          aGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAAGTTTCGc                                                                                                >  1:1516993/1‑149 (MQ=255)
                                            cGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGccc                                                                                              >  1:423204/1‑149 (MQ=255)
                                              gggAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGa                                                                                            >  1:1454642/1‑149 (MQ=255)
                                                gAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTTACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTAGGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATg                                                                                          >  1:297843/1‑149 (MQ=255)
                                                 aaaaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATgg                                                                                         <  2:1278995/149‑1 (MQ=255)
                                                  aaaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGt                                                                                        <  2:606970/149‑1 (MQ=255)
                                                   aaaCGGTCCCGCGTGTCAATGTGTTACGATCGGCTTAAGCGAATAGCCTGAGGCTAACCCAGGGGAAGATCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTGCGCCCGATGGtt                                                                                       <  2:1021576/149‑1 (MQ=255)
                                                   aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                       >  1:66275/1‑149 (MQ=255)
                                                   aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGATATGGAATAAGGCGGTGCCAAACTCGACGTTTCGCCCGATGGtt                                                                                       >  1:1485860/1‑149 (MQ=255)
                                                   aaaCGGGCCAGCGTTTCAAGGTGTTACTCTAGTCAGACGCGAATAGACTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGTGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                       >  1:178306/1‑149 (MQ=255)
                                                    atcGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTCAAGCAAACCGAGGGGAAGTTCAGAAACAAAAAAGCCGGGAATTTCACGGCTTTGTTATGGAATAAGTCGGTACCTTACTCGACGTTTAGCCCGATGGttt                                                                                      >  2:206247/3‑148 (MQ=255)
                                                     aCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGa                                                                                     >  2:834862/1‑149 (MQ=255)
                                                       gggCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGata                                                                                   <  1:1566298/149‑1 (MQ=255)
                                                        ggCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCCACGTTTCGCCCGATGGTTGatat                                                                                  <  1:503089/149‑1 (MQ=255)
                                                         gCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGAtata                                                                                 >  1:508914/1‑149 (MQ=255)
                                                         gCCAGCGTGTCATGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAAGCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGAtata                                                                                 <  1:1455677/149‑1 (MQ=255)
                                                             gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGACGTTCAGATACAACAAAGCCCGGAATTACCCGGCGTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCACGATGGTTGATATAgatt                                                                             >  1:325927/1‑146 (MQ=255)
                                                             gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             >  2:1388932/1‑149 (MQ=255)
                                                             gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             >  2:578998/1‑149 (MQ=255)
                                                             gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTAACCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             >  2:295435/1‑149 (MQ=255)
                                                             gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCAGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGATa                                                                             >  1:1316627/1‑149 (MQ=255)
                                                                tttCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGGTGCTAACCGAGGGGAAGATCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATTGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGc                                                                          <  2:912497/149‑1 (MQ=255)
                                                                 ttCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTCTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCt                                                                         >  1:36541/1‑149 (MQ=255)
                                                                 tgccaGGTGTTCCTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCACATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCt                                                                         <  2:127392/145‑1 (MQ=255)
                                                                    aaGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                      <  2:539101/149‑1 (MQ=255)
                                                                      ggTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGatat                                                                    <  1:1132486/149‑1 (MQ=255)
                                                                       gtgtTACTCTCGTCAGACTCGAATAGCCTGATGCTAACAGATGGGATGTTCAGTTAAAAGAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAGTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATc                                                                   <  2:345138/149‑1 (MQ=255)
                                                                          ttACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCATATACAACAAAGCCGGGAATTACCCTGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACTTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGa                                                                <  1:1181379/149‑1 (MQ=255)
                                                                              tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  1:610665/1‑149 (MQ=255)
                                                                              tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  2:1390632/1‑149 (MQ=255)
                                                                              tctcGTCAGACGCGAATAGCCGGATGCTAACCGCGGGGAAGTTCAGATACAACAAAGCCGGGAAATACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGGTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            <  1:843126/149‑1 (MQ=255)
                                                                               ctcGTCAGACGCGAATAGCCAGATGCTAACCGAGGGGAAGCTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGGTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTg                                                           <  1:928622/149‑1 (MQ=255)
                                                                                  gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACATAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                        <  1:1044271/149‑1 (MQ=255)
                                                                                  gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                        >  1:458933/1‑149 (MQ=255)
                                                                                  gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                        >  2:1192303/1‑149 (MQ=255)
                                                                                  gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCACGATGGTTGATATAGCTACGCTTATATCAGAAGTTGGAc                                                        >  2:1321038/1‑149 (MQ=255)
                                                                                      gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggca                                                    <  1:835060/149‑1 (MQ=255)
                                                                                        cgcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcagg                                                  <  1:1368386/149‑1 (MQ=255)
                                                                                            aaTAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCAGATGGTTGATATAGCTACGCTGATATCAGAAGTTCGACGGCAGGCAcc                                              >  2:229269/1‑149 (MQ=255)
                                                                                               tgCCTGATGCAAACCGAGGGGAAGGTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGGTGGACGGCAGGCACCttg                                           <  1:1418310/148‑1 (MQ=255)
                                                                                               aGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttg                                           >  1:554462/1‑149 (MQ=255)
                                                                                               aGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGATTTGATAAGGAATATGGCGGTGCCGAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttg                                           <  1:1037414/149‑1 (MQ=255)
                                                                                                 ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                         >  2:1137939/1‑149 (MQ=255)
                                                                                                 ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                         >  2:1480156/1‑149 (MQ=255)
                                                                                                     ttGCTAACCGAGGGGAATTTCAGATTCAACAAAGCCGGGAATTACCCGGCTTTGTTATGTAATAAGGCGGTGCCTAACTAGACGTTTCGCTCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGc                                     <  2:1516993/148‑1 (MQ=255)
                                                                                                     attcTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGc                                     <  1:989743/146‑1 (MQ=255)
                                                                                                       gCTAACCGAGGGGAAGTTCAGATACAACAACGCCGGGACTTACCCGGCTTTGTTATGGAATAAGGCGTTGCCTAACTCGACGTTTCGCCGGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGt                                   <  1:953684/149‑1 (MQ=255)
                                                                                                          aaCCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAAGGCAGGCACCTTGTTGTGctgcat                                >  1:1463660/1‑144 (MQ=255)
                                                                                                           aCCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCGTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAATTTGGACGGCAGGCACCTTGTTGTGCGTCAtt                               >  2:885669/1‑149 (MQ=255)
                                                                                                            ccGAGGGCAAGTTCAGAAACAACAAACCCCGGAAAAACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTc                              <  2:1316627/149‑1 (MQ=255)
                                                                                                               aGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTa                           >  1:1175209/1‑149 (MQ=255)
                                                                                                               aGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGGTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTa                           <  1:384170/149‑1 (MQ=255)
                                                                                                               aGGGGAAGATCACAAACAACAAAGCCGGCCATTACCCGGCTTTGTTATGGAATCCGGCGGTGCCTAACTCGACGTTTCGCCCGATCGTTGATAAAGCTACGGTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTa                           <  2:1227787/149‑1 (MQ=255)
                                                                                                                ggggAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAAGATTCGCCCGATCGATCATATAGCTACGATGATATCAGAAGTTGGACGGCAGGCACCTTCTTGTGCGTCATTCGTAt                          >  2:825495/1‑149 (MQ=255)
                                                                                                                   gAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCCTAtttt                       >  2:676774/1‑149 (MQ=255)
                                                                                                                    aaGTTCAGATCCAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGCCGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTa                      <  2:56814/149‑1 (MQ=255)
                                                                                                                    aaGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAATCGACGTTTCGCCCGATGGTTGATATAGCTACGATGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGGAGTTTa                      >  2:276171/1‑149 (MQ=255)
                                                                                                                    aaGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGGGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTa                      <  2:1042414/149‑1 (MQ=255)
                                                                                                                         cAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAg                 <  1:1390632/149‑1 (MQ=255)
                                                                                                                          aGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCTGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCGACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGc                <  2:1374689/149‑1 (MQ=255)
                                                                                                                            ccataacaaAGCCGGGTAATTCCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCAc              >  1:1149795/5‑149 (MQ=39)
                                                                                                                            aTACATCAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGGGCCTAACTCGTCGTTTGGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCAc              <  2:1145812/149‑1 (MQ=255)
                                                                                                                             cataacaaAGCCGGGTAATTCCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACg             >  1:274223/4‑149 (MQ=255)
                                                                                                                              acaacaAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGt            >  2:440085/1‑149 (MQ=255)
                                                                                                                                aacaaATCCGGGAATTACCCTGCTTTGTTATGGAATAAGTCGGTGACTACCTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACAGCAGGCACAATGTTTTGCGTCAATCGTATTTTATGTAGCACTTcc          >  2:1169921/1‑149 (MQ=255)
                                                                                                                                aacaaAGCCGGGAATTACCGGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc          >  1:761934/1‑149 (MQ=255)
                                                                                                                                aacaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCAACTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc          >  2:1516435/1‑149 (MQ=255)
                                                                                                                                 acaaAGCCGGGAATTACCCGGCTTTGTTATGGATTAAGGCGGTTCCTAACTCGACTTTTCGCCCTATGGTTGATATAGCTACGCTTATATCAGAAGTTTGACGGCAGGCACCTTTTTGTGCGTCATTCGTATTTTATGTAGCTCGTccc         <  2:335130/149‑1 (MQ=255)
                                                                                                                                 acaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc         >  1:1475548/1‑149 (MQ=255)
                                                                                                                                 acaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc         >  1:77352/1‑149 (MQ=255)
                                                                                                                                     aGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAg     >  2:737559/1‑149 (MQ=255)
                                                                                                                                       ccGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTGGCACGTCCCGAAggg   >  1:38474/1‑149 (MQ=255)
                                                                                                                                        cGGGAATAACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAgggg  >  1:1159684/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGG  >  NC_000913/2691029‑2691313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: