Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,700,862 G→A 5.8% P46L (CCG→CTG)  acpS ← holo‑[acyl‑carrier‑protein] synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,700,8620GA 5.8% 282.9 / 5.2 104P46L (CCG→CTG) acpSholo‑[acyl‑carrier‑protein] synthase 1
Reads supporting (aligned to +/- strand):  ref base G (55/41);  new base A (2/4);  total (58/46)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 104 total aligned reads displayed.

TCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGC  >  NC_000913/2700718‑2701009
                                                                                                                                                |                                                                                                                                                   
tCGCCCCATAGCCATAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCGTCGCCAGAAAACGCACCGGCtg                                                                                                                                                 >  1:1365039/1‑149 (MQ=255)
   ccgcATAGCCGTAGCCGTGGTTTGCCGAGCTCATCCTTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGCGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAACCGCACCGGCtggtg                                                                                                                                              <  1:551683/146‑1 (MQ=255)
   ccccATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCtggtg                                                                                                                                              <  2:767793/149‑1 (MQ=255)
    cccATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCtggtgg                                                                                                                                             >  1:1443425/1‑149 (MQ=255)
     ccATAGCCGTACCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGAATAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTggtggt                                                                                                                                            <  2:711656/149‑1 (MQ=255)
       aTAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACTCACCGGCTGGTGGTGc                                                                                                                                          >  1:1571857/1‑149 (MQ=255)
          gccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGtt                                                                                                                                       >  1:1512975/1‑149 (MQ=255)
          gccgtagccgtGGGTTGCCGAGCTCATCATTGAATACTTCAACTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCACACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGtt                                                                                                                                       <  2:1006854/149‑1 (MQ=255)
           ccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTTAACGCCAGACCATTGCGGCTCCCGGTGCCACACGCTTTTGCTGCGGCTTCGTTCACAGCACAACGCTGCGCCCGAAACCGCACCTGCTGGTGGTGCGtgt                                                                                                                                      >  1:1047988/1‑147 (MQ=255)
           ccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCAGTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGttt                                                                                                                                      >  2:922848/1‑149 (MQ=255)
            cgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGtttt                                                                                                                                     >  1:189148/1‑149 (MQ=255)
             gtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTc                                                                                                                                    <  1:1393062/149‑1 (MQ=255)
             gtagccggGGTTTGCCGAGCTCCTCATTGAAAACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTc                                                                                                                                    <  1:1053596/149‑1 (MQ=255)
              tagccgtGGTTTGCCGACCTCATCAGTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTct                                                                                                                                   <  1:160044/149‑2 (MQ=255)
               agccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTctg                                                                                                                                  <  2:1003840/149‑3 (MQ=255)
                gccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAg                                                                                                                                 <  1:1254763/149‑1 (MQ=255)
                gccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTctgg                                                                                                                                 <  2:1258913/149‑4 (MQ=255)
                  cgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGAt                                                                                                                               >  1:1121982/1‑149 (MQ=255)
                    tGGTTTTCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAg                                                                                                                             <  2:55583/149‑1 (MQ=255)
                     gggttGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGc                                                                                                                            <  1:1267801/146‑1 (MQ=255)
                     gggttGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCCGATCCCGGTGCCAAACGCCTATGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATacc                                                                                                                            <  1:663189/146‑3 (MQ=255)
                          gCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCAtt                                                                                                                       <  1:168844/149‑1 (MQ=255)
                           ccGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTc                                                                                                                      <  2:602627/149‑1 (MQ=255)
                            cGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGATTCGCCAGAAAACGCCCCGGCTGGTGGTGCGTTTTCCAGATAGCACAtaag                                                                                                                     >  1:926405/1‑146 (MQ=255)
                                  catcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCg                                                                                                               >  2:347732/1‑149 (MQ=255)
                                   atcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCTCTGCCAAACGCTTTTGCTGCGGCTTCTATCACAGCAAAACGCTTCGCCAGAAAACCCACCGGCTGGTGGTGCGTTTTCCAGAAAGCCCAATAGTTATAGc                                                                                                              >  2:1159516/1‑149 (MQ=255)
                                   atcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGc                                                                                                              >  2:1257468/1‑149 (MQ=255)
                                      atTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCAACGGCTGGTGTTGCGTTTTCCAGAATGCCCATCCGTTATAGATTa                                                                                                           >  2:1468549/1‑149 (MQ=255)
                                      atTGAATACTTCAAATTGATTAAACGCCAGACCATGGGGGAGCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCTAAACGCTTCGCCAGAAAACGCACCTGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTa                                                                                                           <  2:302844/149‑1 (MQ=255)
                                        tGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCCCCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAAt                                                                                                         >  1:883741/1‑149 (MQ=255)
                                         gAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCGTAATa                                                                                                        >  1:887664/1‑149 (MQ=255)
                                              cTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcgg                                                                                                   >  1:634890/1‑149 (MQ=255)
                                               ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCCTTCGTTATCGCTTAATACgcggc                                                                                                  >  2:928923/1‑149 (MQ=255)
                                               ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                                  >  1:133375/1‑149 (MQ=255)
                                               ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                                  >  2:144019/1‑149 (MQ=255)
                                               ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCATTCGTTAGCGCTTAATACgcggc                                                                                                  >  1:376565/1‑149 (MQ=255)
                                                tCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGTAGGTGCGTTATCCAGATAGCCCATTCGTTATAGCTTAATACtcggcg                                                                                                 >  1:1029086/1‑149 (MQ=255)
                                                tCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGGGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggcg                                                                                                 >  1:1426412/1‑149 (MQ=255)
                                                 cAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGt                                                                                                <  2:1512975/149‑1 (MQ=255)
                                                   aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGc                                                                                              <  1:811265/149‑1 (MQ=255)
                                                      tGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAg                                                                                           <  1:1259541/149‑1 (MQ=255)
                                                       gATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAgg                                                                                          <  2:677285/149‑1 (MQ=255)
                                                       gATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGGTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAgg                                                                                          >  1:269702/1‑149 (MQ=255)
                                                          tAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGATTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGACGTGCCAGGCGa                                                                                       >  1:423112/1‑149 (MQ=255)
                                                           aaaCGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGAt                                                                                      >  1:1344123/1‑149 (MQ=255)
                                                            aaCGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACCGCAAAACGCTTCGCCCGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGCTAGCCCATTCGTTATCGCTTATAACGCGGCGTGCCAGGCGATc                                                                                     >  2:1214137/1‑149 (MQ=255)
                                                             aCGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCa                                                                                    >  2:228005/1‑149 (MQ=255)
                                                               gCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCAcc                                                                                  >  1:493639/1‑149 (MQ=255)
                                                                 cAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                                >  1:899917/1‑149 (MQ=255)
                                                                   gACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGag                                                                              >  2:1293549/1‑148 (MQ=255)
                                                                              aTCCCGGCGCCAAACGCGTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCAc                                                                   <  1:74677/149‑1 (MQ=255)
                                                                                   tgTGCCAAACGCTTTTGCTGCGGATTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTACAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCtt                                                              <  2:958943/148‑1 (MQ=255)
                                                                                   ggTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCtt                                                              <  2:189148/149‑1 (MQ=255)
                                                                                   ggTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCtt                                                              >  2:1221976/1‑149 (MQ=255)
                                                                                   ggTGCCAAACGCGTGTGCTGCGGCGTCTTTCACAGCAAAACGCTGCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCAATCCTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCtt                                                              <  2:964653/149‑1 (MQ=255)
                                                                                       ccAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGAt                                                          <  2:8107/149‑1 (MQ=255)
                                                                                         aaaCGTTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                        <  2:887503/149‑1 (MQ=255)
                                                                                         aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                        >  1:1150465/1‑149 (MQ=255)
                                                                                         aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGATGGTGGTGCGTTTTCCAGATAGCACATTCGTTATCGCTTAATAAGCGTCGTGCCAGTCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                        >  1:1058485/1‑149 (MQ=255)
                                                                                         aaaCGCTTATGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCAGCTGGTGGTGCGTTTTCCAGAAAGCCCATTCGTTATCTCTTAATACGCGTACTGCCAGGCGATCACCGGATCGGTCGATCACCGCTTCGATgc                                                        >  2:1281247/1‑149 (MQ=255)
                                                                                          aaCGCTTTTGCTGCGGCTTCTTTCACAGCAACACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcg                                                       >  2:1300548/1‑149 (MQ=255)
                                                                                           aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGAGCGATCACCGCTTCGATgcga                                                      <  1:33302/149‑1 (MQ=255)
                                                                                           aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcga                                                      <  1:603634/149‑1 (MQ=255)
                                                                                           aCGCGTTTGCTGCGGCTTCATTCACAGCAAAACGCTTCGACAGAAACCGCTCCGGCTGGGGGTGCGGTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcga                                                      <  1:103020/149‑1 (MQ=255)
                                                                                               ttttGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGAGCACCGGATCGGGCGATCACCGCTTCGATgcgagcga                                                  >  1:1506291/1‑149 (MQ=255)
                                                                                                 ttGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAtc                                                <  2:1365039/149‑1 (MQ=255)
                                                                                                      gcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcac                                           >  1:1306269/1‑149 (MQ=255)
                                                                                                      gcGGCTGCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGGTATCGCTTAATACGCGGCGTGCCAGGCGATCACCTGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcac                                           <  2:605491/149‑1 (MQ=255)
                                                                                                       cGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcaca                                          >  1:631733/1‑149 (MQ=255)
                                                                                                         gCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAat                                        >  1:375175/1‑149 (MQ=255)
                                                                                                          cttcttTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGATATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGTGCGAACACCGCTTCGATGCGAGCGATCTCCACAata                                       >  2:331325/1‑149 (MQ=255)
                                                                                                              tttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCCCCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCg                                   >  1:163144/1‑149 (MQ=255)
                                                                                                              tttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCg                                   >  2:1093453/1‑149 (MQ=255)
                                                                                                               ttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACTATATCCGt                                  >  1:1324981/1‑149 (MQ=255)
                                                                                                               ttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGt                                  <  2:1150465/149‑1 (MQ=255)
                                                                                                                tCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTg                                 <  2:133375/149‑1 (MQ=255)
                                                                                                                 cacaTCAAAACGATGCGCCAGAAAACGCTCAGGCAGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGTCGGGCCAGGCGATCACCGGCTCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGGGc                                <  1:849682/149‑1 (MQ=255)
                                                                                                                 cacaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGc                                >  1:327370/1‑149 (MQ=255)
                                                                                                                 cacaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTGCGATGCGAGCGATCTCCACAATATCCGTGc                                <  1:725382/149‑1 (MQ=255)
                                                                                                                  acaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGcc                               >  2:1102738/1‑149 (MQ=255)
                                                                                                                   caGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTTATACGCGGCGTTCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGCTGCGCGCGATCTCCACAATATCCGTGCCt                              >  1:1225702/1‑149 (MQ=255)
                                                                                                                   caGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGccg                              >  2:453028/1‑148 (MQ=255)
                                                                                                                    aGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTa                             >  1:1096636/1‑149 (MQ=255)
                                                                                                                    aGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGAATACCAGATAGCCCATTCGTTATAGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGTGCGATCACCGCTTCGATGCGAGCGATCTCCACAAAATCCGTGccga                             >  2:1013052/1‑147 (MQ=255)
                                                                                                                    aGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCATTCGTTAGCGCTTAATACGCGGCGTGCCAGGTGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGCTCTCCACAAGATCCGTGCCTa                             >  2:1007804/1‑149 (MQ=255)
                                                                                                                       aaaaCGCTTCGACACAAAACGCACCGGCTGGTGGTGCGTTTGCCAGATAGCCCATTCGTGATCGCTTAATACGCGGCGGGTCAGCGGATCAACGGATCTGGCGATCACCTCGTCGAGGCGAGCGATCTCCTCAATATCCGTGCCTAAAc                          <  2:846143/149‑1 (MQ=255)
                                                                                                                         aaCGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCt                        >  1:948193/1‑149 (MQ=255)
                                                                                                                            gCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAat                     <  2:1121982/149‑1 (MQ=255)
                                                                                                                             cTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAata                    <  2:1285171/149‑1 (MQ=255)
                                                                                                                                 gCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGc                <  1:1327258/149‑1 (MQ=255)
                                                                                                                                 gCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGc                <  1:1468549/149‑1 (MQ=255)
                                                                                                                                  ccAGACAACTCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGcc               <  1:1528261/149‑1 (MQ=255)
                                                                                                                                   cAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCa              <  2:1443425/149‑1 (MQ=255)
                                                                                                                                     gAAAACGCACCACCAGCCGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCCGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTACACCTAATATTGCCAtt            >  1:8342/1‑149 (MQ=255)
                                                                                                                                        aaCGCACTGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAGCCTAATATTGCCATTAGc         >  2:1099187/1‑149 (MQ=255)
                                                                                                                                        aaCGCACCGGCTGGTGGTGCGTTTTCCAGCTAGCCCATTCGTTATCGCGTAATACGCGGCGTGCCAGGCGATCCCCGGCTCGGGCGATCACCGCTTCGATGCGAGCGGTCTCCACATTATCCGTGCCTTCACCGAATATTGCCATTGGc         >  1:1025869/1‑149 (MQ=255)
                                                                                                                                        aaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATTTCCGTGCCTACACCTAATATTGCCATTAGc         >  1:480720/1‑149 (MQ=255)
                                                                                                                                         aCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTCGcc        >  2:854947/1‑149 (MQ=255)
                                                                                                                                         aCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGcc        >  1:487079/1‑149 (MQ=255)
                                                                                                                                               cGGCTGGTGCTGCCTTTTACAGATAGCCCATTCGTTATCGCTAAATACGCGTCGTGCCAGGCGATCACCGGATCGCGCGATAACCGCTTCGAAGCGACCGATCTCCACAATATCCGTGCCTAAACCTAAAATAGACAATAGCCACgcgc  >  2:1566989/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGC  >  NC_000913/2700718‑2701009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: