Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,581 G→T 8.5% intergenic (‑424/+19) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,5810GT 8.5% 205.0 / 6.9 85intergenic (‑424/+19)rrsG/clpB16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (37/39);  new base T (6/2);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGA  >  NC_000913/2731433‑2731729
                                                                                                                                                    |                                                                                                                                                    
gttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCg                                                                                                                                                      <  1:159034/149‑1 (MQ=255)
gttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCg                                                                                                                                                      <  1:397348/149‑1 (MQ=255)
       cGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCt                                                                                                                                               >  2:837269/1‑149 (MQ=255)
           tCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAATCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                                                                                                           <  2:824292/149‑1 (MQ=255)
            cAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAAAATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCTTCGGGGCTCGttt                                                                                                                                          <  1:1478617/149‑1 (MQ=39)
             cgtggtgGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtttt                                                                                                                                         <  2:1072307/148‑1 (MQ=255)
             aGTGGTGGCGCATTATAGTGAGTTATTCTTGCCTGACATGCGAAATATATAAAAACTTTATCGTTCGCTAAATTTTCATTCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATATACATAAACGAGCCCCGAAGTGCTCGtttt                                                                                                                                         <  2:560763/149‑1 (MQ=255)
             aGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGTAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCTTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtttt                                                                                                                                         >  2:539013/1‑149 (MQ=255)
             aGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtttt                                                                                                                                         <  1:806472/149‑1 (MQ=255)
              gtggtgGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTATCAGGCAAAACATCTTAAATATAGTCTTTTCCGTAAAACTTATAGACAAAAACGAGCCCCGAAGGCCTCGTTTTa                                                                                                                                        >  2:1489650/1‑149 (MQ=255)
               tggtggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCACGAAGGGCTCGTTTTAt                                                                                                                                       >  1:1243089/1‑149 (MQ=255)
                      gcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAATAACGAGCCCCGAAGGGCTCGTTTTATCATTTAc                                                                                                                                >  2:190122/1‑149 (MQ=255)
                      gcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTAc                                                                                                                                <  1:1542482/149‑1 (MQ=255)
                      gcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCGAACTTATAGACAAAAACGAGCCCCGACGGGCTCGTGTTATCATTTAc                                                                                                                                >  2:1131194/1‑149 (MQ=255)
                      gcATTATAGGGAGTTATTCAGGCATGACAATCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACTAGCCCCGAAGGGCTCGTTTTATCATTTAc                                                                                                                                <  2:582665/149‑1 (MQ=255)
                       cATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACt                                                                                                                               <  1:216467/149‑1 (MQ=255)
                       cATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAAATTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACt                                                                                                                               <  1:1041088/149‑1 (MQ=255)
                        aTTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGTCAAAACATATTAAATTTAGTCTTTTCAGTCTACCTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTAATg                                                                                                                              >  2:850228/1‑149 (MQ=255)
                           ataGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTCTTATCATTTACTGGAc                                                                                                                           <  1:222108/149‑1 (MQ=255)
                            taGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTGTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGATCCACGAAGGGCTCGTTTTATCCTTTCCTGGACg                                                                                                                          >  2:381241/1‑149 (MQ=255)
                               ggAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCg                                                                                                                       >  2:906686/1‑149 (MQ=255)
                                taGTTATTTCGGCCTGAGAATGTAAAAATATAAACACTTTATCGTTCGCTCACTTTTCATGCAAAACATCTTAAATTTAGTCTTTTCCGTCTAACTTATATACAAAAACGAGCCCCGAATGGCTCGTTTTATGATTTACTGGACGGCGa                                                                                                                      <  2:104658/148‑1 (MQ=255)
                                gAGTTATTCCGGCCTGACAAGCGTAAAATATAAAAAATTTATCGGTCTCTAACTTTTCAGTCAAAACATCTTAAATATACTCTTTTTGGGCTTACTTTTAGACAATAACGAGCCCCGATGTGCTCGTTTTATCATTTTCTGGACGGCGa                                                                                                                      <  1:1015722/149‑1 (MQ=255)
                                 aGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGAc                                                                                                                     <  1:30761/149‑1 (MQ=255)
                                     aTTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTAATGGACGGCGACAATc                                                                                                                 >  1:1529232/1‑149 (MQ=255)
                                      ttCCGGCCTGACAAGCGAAAAATATAAACACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATTGTCTGTTCCGGCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACATTcc                                                                                                                >  2:1094801/1‑149 (MQ=255)
                                      ttCCGGCCTGACAAGCGAAAAATATAAAACCTTTATCGTTCGCTCCCTGTTCCGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCTAAGGGAGAGTTTTATCATTTACTGGCAGGCTACAAGcc                                                                                                                >  2:1100519/1‑149 (MQ=255)
                                      ttCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATcc                                                                                                                <  2:807103/149‑1 (MQ=255)
                                       tCCGGCCTGACTAGCGAAAAATATAATAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTTAATATTGTCTTTTCCGTCTTATTTATAGACAAAAACCAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCg                                                                                                               <  1:1374882/149‑1 (MQ=255)
                                       tCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCATGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGTGCTCGTTTTATCATTTACTGGACGGCGACAATCCg                                                                                                               <  1:474087/149‑1 (MQ=255)
                                           gCCTGACAAGCGAAAAATATAAAAACTTTATAGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCt                                                                                                           <  2:882288/149‑1 (MQ=255)
                                             cttaCAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTATCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAACCGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTc                                                                                                         <  2:1506315/146‑1 (MQ=255)
                                               gACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAt                                                                                                       >  1:404091/1‑149 (MQ=255)
                                               gACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAt                                                                                                       >  2:1172311/1‑149 (MQ=255)
                                                   aGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCATGCAAAACATATTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAAc                                                                                                   <  2:970082/149‑1 (MQ=255)
                                                    gCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACt                                                                                                  >  1:762697/1‑149 (MQ=255)
                                                    gCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCCGTCTTCATTAACt                                                                                                  >  1:807029/1‑149 (MQ=255)
                                                      gAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTc                                                                                                >  2:79707/1‑149 (MQ=255)
                                                          aaTATAACAACTTTATCGTTCGCTCACTTATCAGTCAAAACATCTTAAATATAGTCTTTTACGTATAACTTATATACAAAAACGAGCCCCGAAGTGCTCGTTTTATCATTTACTGGACGGCGACAATGCGGTCTTCATTAACTTCCAgg                                                                                            <  2:525252/149‑1 (MQ=255)
                                                               aaaaaCTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAAt                                                                                       >  1:1316184/1‑149 (MQ=255)
                                                               aaaaaCTTTATCGTTCGCTCACGTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACCAGCCCCCAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGaaa                                                                                       >  1:981414/1‑148 (MQ=255)
                                                                    cTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACTGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACtt                                                                                  >  2:837562/1‑149 (MQ=255)
                                                                     tttATCGTTCGCTCAATTTTCAGGCAAAACTTCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCTAAGGGCTCGTTTTATCATTTAGTGGACGGAGACATTCCGGTCTTCATTAACTTCCAGGCGAATCACttt                                                                                 <  2:366828/149‑1 (MQ=255)
                                                                      ttATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGTGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTa                                                                                >  2:339254/1‑149 (MQ=255)
                                                                          cGTTCGCTCACTTTTCAGGCAAAACATCTTAAAAATCGTCTTTTCCGTCTAACTTATAGACAAAAACGCGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGGCTTCATTAACTTCCAGGCGAATCACTTTACCCg                                                                            <  1:1553422/149‑1 (MQ=255)
                                                                            ttCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGa                                                                          >  2:775683/1‑149 (MQ=255)
                                                                             tCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGaa                                                                         <  1:837269/149‑1 (MQ=255)
                                                                              cGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAAc                                                                        <  1:978050/149‑1 (MQ=255)
                                                                              cGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGACCCCCGACTGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAAc                                                                        <  1:1428466/149‑1 (MQ=255)
                                                                                  cACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAAt                                                                    <  2:1118400/149‑1 (MQ=255)
                                                                                   aCTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAAtt                                                                   >  1:76659/1‑149 (MQ=255)
                                                                                      tttCAGGCAACACATCTTAAATATAGTCTTTTCCGTCTAACTTTTAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGGCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCAc                                                                <  2:1535995/149‑1 (MQ=255)
                                                                                              aaaaCATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGt                                                        >  2:770118/1‑149 (MQ=255)
                                                                                                   aTCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTg                                                   <  1:457327/149‑1 (MQ=255)
                                                                                                      ttAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtg                                                <  2:106079/149‑1 (MQ=255)
                                                                                                       tAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCACGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtgt                                               <  1:294606/149‑1 (MQ=255)
                                                                                                               ttCTTTTCAGTCTAACTTATAGACAAAATCGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTCCCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCgg                                       <  1:850228/148‑1 (MQ=255)
                                                                                                               gTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCgg                                       <  2:404091/149‑1 (MQ=255)
                                                                                                                tCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTGTTATCATTTACTCGCCGGCGCCAAACCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCggg                                      >  1:869148/1‑149 (MQ=255)
                                                                                                                tCTTTTCCGGCTAACTTATAGACAAAAACGCGCCCCGAAGGGCTCGTTTTATCATTTAATGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGAATTTGCTGTGCCAGCggg                                      <  2:1289102/149‑1 (MQ=255)
                                                                                                                  ttttCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGCCAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCCGACAGTATTTGCTGTGCCAGCGGGtt                                    >  1:1412209/1‑149 (MQ=255)
                                                                                                                  ttttCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATAACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGtt                                    >  1:56686/1‑149 (MQ=255)
                                                                                                                    ttCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCTGGtttt                                  <  1:1363107/149‑1 (MQ=255)
                                                                                                                     tCCGTCTAACTTATAGACAAAACCGAGCCCCGAAGGGCTCGTTTTATCAATTACTGGACGGCGACAATCCGGTCATCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTc                                 <  2:346311/149‑1 (MQ=255)
                                                                                                                     tCCGTCTAACTAATAGACAAAAACGAGCACCGAAGGGCGAGTTTTATCATTTACTGGACGGAGACAATCCGGTCTTCATTAACTTGCAGGCGAATCACTTTACCCGGAACCAATTCAGCAGACAGTATTTGCTGTGCCAGCGGGTTTTc                                 <  1:1489650/149‑1 (MQ=255)
                                                                                                                        gTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGAt                              >  2:778380/1‑149 (MQ=255)
                                                                                                                         tCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAAATTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATc                             >  2:355487/1‑149 (MQ=255)
                                                                                                                           tAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctg                           >  2:241946/1‑149 (MQ=255)
                                                                                                                           tAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctg                           >  2:967517/1‑149 (MQ=255)
                                                                                                                           tAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGAAAATCCGGTCTTAATTAACTTCAATGCGAATCACTTTACCCGGAACCAATTAACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctg                           >  2:622037/1‑149 (MQ=255)
                                                                                                                            aaCTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctgc                          <  2:805353/149‑1 (MQ=255)
                                                                                                                              cTTATAGACAAAAACGAGCCCCGAAGGGCGCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGACCCAATTCACCAGACAGTATTTGCTGGGCCAGCGGGTGTTCGCTctgctg                        >  1:1529693/1‑149 (MQ=255)
                                                                                                                               ttATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctgctgc                       >  1:587706/1‑149 (MQ=255)
                                                                                                                                   tctcAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCATTTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATctgctgctgta                   <  1:327575/146‑3 (MQ=255)
                                                                                                                                      ataaaaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTGTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTg                >  1:426744/3‑149 (MQ=255)
                                                                                                                                      ataaaaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCGCTTTACCCGGAACCAATTCACAAGACAGTATTTGCTGTGCCAGGGGGTTTTCGATCTGGTGCTGAATTg                >  1:418322/3‑149 (MQ=255)
                                                                                                                                       aaaaaCGAGCACCGAAGGGCTCGTTTTATCATTTACTGTACGGCGACAATCCGCTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGAATTTGCTGTGCCAGCGGGGTTTCGATCTGCTGCTGAAtaga               >  2:327575/1‑146 (MQ=255)
                                                                                                                                        aaaaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGACTCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTgaa              >  1:1512614/1‑147 (MQ=255)
                                                                                                                                          aaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACg            >  1:224292/1‑149 (MQ=255)
                                                                                                                                          aaCGAGCCCTTCGGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACg            >  1:1288449/1‑149 (MQ=255)
                                                                                                                                          aaCGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACg            >  1:1263176/1‑149 (MQ=255)
                                                                                                                                             gAGCCCCGAATGGCTCGTTTTATCATTTACTTGACTGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGttt         <  2:56686/149‑1 (MQ=255)
                                                                                                                                               gCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCa       <  1:906686/149‑1 (MQ=255)
                                                                                                                                                ccccGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCag      <  2:1316184/149‑1 (MQ=255)
                                                                                                                                                ccccGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAAATCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCag      >  1:453043/1‑149 (MQ=255)
                                                                                                                                                 cccGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCaga     >  1:973875/1‑149 (MQ=255)
                                                                                                                                                   cGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCCGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAgg   >  1:1408593/1‑149 (MQ=255)
                                                                                                                                                    gAAGGGCTCGTTTTATCATTTACTGGACGGCGCCAATCCGGTCTTCATTAACTGCCAGGCGAATCTCTTTACCCGGAACCAATTCACCAGCCAGTATTTGCTGTGCCAGCGGGTGTTCGATCTGAGGCTGAATTGCAAGTTTCagatgc  >  2:1329212/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGA  >  NC_000913/2731433‑2731729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: