Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,754,944 A→G 6.6% N17D (AAC→GAC) ‡ smpB → tmRNA‑binding trans‑translation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,9440AG 6.6% 305.2 / 9.1 106N17D (AAC→GAC) ‡smpBtmRNA‑binding trans‑translation protein
Reads supporting (aligned to +/- strand):  ref base A (53/45);  new base G (2/5);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 108 total aligned reads displayed.

GCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTT  >  NC_000913/2754797‑2755092
                                                                                                                                                   |                                                                                                                                                    
gcTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTaa                                                                                                                                                     <  2:801967/149‑1 (MQ=255)
 cTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAAc                                                                                                                                                    <  1:404105/149‑1 (MQ=255)
     gTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAgc                                                                                                                                                >  2:32448/1‑149 (MQ=255)
       aaCATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAgcgc                                                                                                                                              >  1:1392314/1‑149 (MQ=255)
        aCAACGGGTTCATGCTAAGATAGAGCCTGGTCCCCCGCCGGATTGATATGTGGTGTTTTCGATTTCAGATAACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTTGTTCAGCGACCATCGCGCTTGTTAAgcgcg                                                                                                                                             <  2:1034732/149‑1 (MQ=255)
         cATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTGTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAgcgcgc                                                                                                                                            <  2:76098/149‑1 (MQ=255)
         aaTCGGGTTCATGCTACGATAGAGCCATGTCCCCCGCAGGATTGATATGGGGTCTCATCGATTTCAGATTACCGATGATTCACGACTCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTGTTAAgcgcga                                                                                                                                            <  2:891145/148‑2 (MQ=255)
          aTCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGcc                                                                                                                                           >  1:436216/1‑149 (MQ=255)
          aTCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGAGTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACCAGCGCGcc                                                                                                                                           >  1:1081298/1‑149 (MQ=255)
          aTCGGGTTCATGCTAAGATAGAGCCTTGTCCAACGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGAAGCTTATGACGAAGAAAAAAGCACATAACCCTGGTTCAGCGCCCATCGCGCTTAACAACCGCGcc                                                                                                                                           >  1:1372658/1‑149 (MQ=255)
           tCGGGTTCCAGCTAAGATAGAGCCTGGTCCCCCGCAGGATTGCTATGGGGTGGTGTCGATTTCAGATTACCGATGCTTCACGACGCGTATGACTAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTGTTAAgcgcgatg                                                                                                                                          <  2:1039095/149‑4 (MQ=255)
           tCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATCGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGccc                                                                                                                                          >  2:266401/1‑149 (MQ=255)
              ggTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTGTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTGTTAAgcgcgatggtc                                                                                                                                       <  1:1416469/149‑7 (MQ=255)
               gTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGTGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTGTTAAgcgcgatggtcg                                                                                                                                      <  2:1261586/149‑8 (MQ=255)
                 tCATGCTAAGATAGAGCCTTGTCCCCCGCACGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAACAAGCACATAAACCTGGTTCAGCGCCCATCGCGCTTAACACGCGCGCCCGTCACg                                                                                                                                    >  1:1378218/1‑149 (MQ=255)
                   aTGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGaa                                                                                                                                  >  1:476803/1‑149 (MQ=255)
                     gCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATa                                                                                                                                <  2:40136/149‑1 (MQ=255)
                       tAAGATAGCGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACt                                                                                                                              >  2:64249/1‑149 (MQ=255)
                       tAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACt                                                                                                                              >  2:1367225/1‑149 (MQ=255)
                        aaGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGCCCATCGCGCTTAACAAGCGCGCCCGTCACGAATACtt                                                                                                                             >  1:76923/1‑149 (MQ=255)
                          gATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTa                                                                                                                           >  2:1222054/1‑149 (MQ=255)
                           aTAGAGCCTTGTCCCCCGCAGGATTGATATGGGTTGTTTTCGATTTCAGTTTACCGATTATTAACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAAAAAGCGCGCCCGTCACGAATACTTTAt                                                                                                                          >  1:863059/1‑149 (MQ=255)
                           aTAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTAt                                                                                                                          <  2:1145954/149‑1 (MQ=255)
                           aTAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTAt                                                                                                                          <  1:393682/149‑1 (MQ=255)
                            tAGAGCCGTGTCCCCCGCAGGATTGATATGGGGTGTTTTAGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGAACAGCGCGCTTAACAAGCGCGCCCCTCAGGAATACTTTATc                                                                                                                         >  1:25991/1‑149 (MQ=255)
                              gagCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCga                                                                                                                       >  1:683138/1‑149 (MQ=255)
                               agCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATAc                                                                                                                               <  1:207277/140‑1 (MQ=255)
                                     gTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAATAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAagag                                                                                                                <  1:966045/149‑1 (MQ=255)
                                      tCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCACATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTAAGCGACCATCCCGCTAAACAAGCCCGCCCGTCACGAATACTGTATCGAagaagata                                                                                                               >  2:844739/1‑147 (MQ=255)
                                       cccccGCAGGATTGATATGTGGTGTTTTCGATTTCAGATTACCGTTGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGtt                                                                                                              <  2:506944/149‑1 (MQ=255)
                                       cccccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGtt                                                                                                              <  1:1367225/149‑1 (MQ=255)
                                        ccccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAATCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTc                                                                                                             >  2:1560426/1‑149 (MQ=255)
                                         cccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCg                                                                                                            >  2:1500374/1‑149 (MQ=255)
                                         cccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCg                                                                                                            >  2:481499/1‑149 (MQ=255)
                                           cGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGaa                                                                                                          <  2:455799/149‑1 (MQ=255)
                                            gCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAg                                                                                                         <  2:433721/149‑1 (MQ=255)
                                               ggATTGATATGGGGTGTATTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCgg                                                                                                      >  2:468321/1‑149 (MQ=255)
                                                gATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCggg                                                                                                     >  2:903848/1‑149 (MQ=255)
                                                gATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTAACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGCCCATCGCGCTTAACAAGCGCGCCCGTCCCGTAGACTTTATCGAAGACGAGTTCGAAGCggg                                                                                                     >  2:1018440/1‑149 (MQ=255)
                                                 aTTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGa                                                                                                    <  2:783582/149‑1 (MQ=255)
                                                     atatGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTg                                                                                                >  1:1512851/1‑149 (MQ=255)
                                                     atatGGGGTGTTTTCGATTTCAGATTACCGATGATTAACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGCCCCTCGCGCTGAACAAGCGCGCCCGGCACGAATACTTTATCGAAGAACAGTTCGAAGAGGGACTTg                                                                                                >  2:686732/1‑149 (MQ=255)
                                                       atGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGcc                                                                                              <  1:823782/149‑1 (MQ=255)
                                                       atGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGcc                                                                                              <  1:1500374/149‑1 (MQ=255)
                                                       atGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTGCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGcc                                                                                              <  1:903298/149‑1 (MQ=255)
                                                        tGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGCCCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGccc                                                                                             >  2:709732/1‑149 (MQ=255)
                                                          gggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTg                                                                                           >  1:997944/1‑149 (MQ=255)
                                                           ggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGc                                                                                          <  2:775653/149‑1 (MQ=255)
                                                           ggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGAGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGc                                                                                          <  2:349708/149‑1 (MQ=255)
                                                               ttttCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAgg                                                                                      >  2:379099/1‑149 (MQ=255)
                                                                   cGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTgg                                                                                  <  1:308660/149‑1 (MQ=255)
                                                                       ttCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAg                                                                              <  2:268159/149‑1 (MQ=255)
                                                                       ttCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACAAAAACCTCGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAg                                                                              <  1:1012774/149‑1 (MQ=255)
                                                                          aGATAACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAACCCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGACTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTa                                                                           <  1:443236/149‑1 (MQ=255)
                                                                           gATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTaa                                                                          >  2:421632/1‑149 (MQ=255)
                                                                           gATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTaa                                                                          <  1:903848/149‑1 (MQ=255)
                                                                            aTTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGAGCCCGTCACGAATACTTTATCCAAGAAGATTTAGAAGCGGGAATTCCCCTGCAATGCTGGGATGTTaaa                                                                         >  1:1019595/1‑149 (MQ=255)
                                                                               aCCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATcc                                                                      <  1:32448/149‑1 (MQ=255)
                                                                                ccGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATccc                                                                     <  1:1463676/149‑1 (MQ=255)
                                                                                 cGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCt                                                                    >  1:434150/1‑149 (MQ=255)
                                                                                 cGATGATGCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGACGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCt                                                                    <  1:104818/149‑1 (MQ=255)
                                                                                   atgatTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTgc                                                                  >  1:458839/1‑149 (MQ=255)
                                                                                       ttCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTgcgcgc                                                              >  2:813347/1‑149 (MQ=255)
                                                                                        tCACGACGTTTAATACGAAGAAAAAAGCAGATAAAGGTGGTTCAGTGACCATCGCGGTTAACAAGCGCGGCCGTGACGAATACTTTATGGATGAAGTTTTCTATGCGGGACTTGCCCTGCAAGGCTGGGATGTTTTATCCCTgggggcc                                                             <  2:934420/149‑7 (MQ=255)
                                                                                        tCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGGCACGAATACTATATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCa                                                             <  2:278122/149‑1 (MQ=255)
                                                                                         cACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAg                                                            <  2:158716/149‑1 (MQ=255)
                                                                                          acgacgCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAgg                                                           >  1:1532424/1‑149 (MQ=255)
                                                                                            gacgCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCTCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGTaa                                                         >  2:952378/1‑149 (MQ=255)
                                                                                            gacgCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGaa                                                         >  1:334648/1‑149 (MQ=255)
                                                                                                      aCGAATAAAAAATCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAatat                                               <  1:474227/149‑1 (MQ=255)
                                                                                                        gaagaaAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTAAATGGAAGAAGAGTAAGAAGCTGGACTTCCCCTGCAACGCTGGGAAGTTAACTCCCTGCGCGCAGCAAAAGCCAATATCa                                             >  2:844581/1‑149 (MQ=255)
                                                                                                         atgaaAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAAACCCTGCGCGCAGGAAAAGCCAATATCAg                                            >  2:1525860/3‑149 (MQ=255)
                                                                                                          agaaAAAAGCACATAAACCTGGTTCAGCGACCATCTCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGc                                           <  2:127842/149‑1 (MQ=255)
                                                                                                          agaaAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGc                                           <  1:669027/149‑1 (MQ=255)
                                                                                                           gaaAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCg                                          >  2:472453/1‑149 (MQ=255)
                                                                                                           gaaAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTGTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAACTCCCTGCGCGCAGGAAAAGCCAATATCAGCg                                          >  2:66863/1‑149 (MQ=255)
                                                                                                              aaaaGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACCAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATACCTGCGCGAAGGAAAAGCAAATATCAGCGACa                                       >  1:1440852/1‑149 (MQ=255)
                                                                                                              aaaaGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTGTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACa                                       >  2:1285983/1‑149 (MQ=255)
                                                                                                                aaGCACTTAAACCTGGGTCAGCGACCAGCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGACGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGc                                     <  2:1045799/149‑1 (MQ=255)
                                                                                                                 aGCACATACACCTGGTTCTGCGTCCTTCGCGCGTAACAAGCGCGCCCTTCACGAATACTTTATCGAAGAAGAGGTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCTCAGGAAAAGCCAATATCAGCGACAGCt                                    <  2:922918/149‑1 (MQ=255)
                                                                                                                 aGCACATAAAGGTGGTTCAGCGACCATCGAGCTTACCAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGATTTCGAAGCGGGACTTGCCCTGCTAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGTCAGCt                                    <  2:1019595/149‑1 (MQ=255)
                                                                                                                      aTAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTc                               >  2:417358/1‑149 (MQ=255)
                                                                                                                      aTAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTc                               <  2:997944/149‑1 (MQ=255)
                                                                                                                      aTAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGAGGGACTTGCCCAGCAAGGCTGGGAAGTTAAATCCCTGCTAGAAGTAAAAGCCCATATCACCGACAGCAACGTc                               >  1:41459/1‑149 (MQ=255)
                                                                                                                            cTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGACTACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGATGTTAAATCCCTGCGCGCAGGAAAAGCAAATATAAGCGACAGCTACGTACTTCTg                         >  2:881356/1‑149 (MQ=255)
                                                                                                                            cTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTg                         >  1:1438661/1‑149 (MQ=255)
                                                                                                                             tGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGc                        <  1:1222054/149‑1 (MQ=255)
                                                                                                                             tGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTATATCGAAGAAGAGATCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGc                        <  1:875355/149‑1 (MQ=255)
                                                                                                                               gtgcAGCGACCATCGCGCTTAACAAGCGCGCCAGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATAGCAGCGACAGCTACGTCCTTCTGCGt                      <  2:514314/146‑1 (MQ=255)
                                                                                                                                 tCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGa                    <  2:1542367/149‑1 (MQ=255)
                                                                                                                                 tCAGCGACCATAGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCTAAGAATATTTCGAAGCGGGACTTGCCCTGCAAGGCTGGTAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGa                    <  1:844739/149‑1 (MQ=255)
                                                                                                                                  cAGCGACCATCGCGCTTATCTAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGAc                   <  2:135850/149‑1 (MQ=255)
                                                                                                                                    gCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACgg                 >  1:158828/1‑149 (MQ=255)
                                                                                                                                        ccATCTCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGATGCTGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGagag             <  2:681092/149‑1 (MQ=255)
                                                                                                                                           gcgcgCTTGTTAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCTCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCa          >  1:624904/2‑149 (MQ=255)
                                                                                                                                           gcgcgCTTGTTAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCa          >  1:465403/2‑149 (MQ=255)
                                                                                                                                              cgcTTCACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGACCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCAttt       <  2:98118/149‑1 (MQ=255)
                                                                                                                                              cgcTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAATCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCAttt       <  2:1392314/149‑1 (MQ=255)
                                                                                                                                                cTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGAAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGACAATATCAGCGACAGCTACGTCCATCTGCGAGACGGAGAGGCATTTCt     >  2:1319016/1‑149 (MQ=255)
                                                                                                                                                   aaCAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGtt  <  2:659892/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTT  >  NC_000913/2754797‑2755092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: