Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,768,620 G→A 8.3% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,768,6200GA 8.3% 223.2 / 7.7 84intergenic (+47/‑45)yfjP/yfjQCP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (34/43);  new base A (5/2);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTG  >  NC_000913/2768476‑2768767
                                                                                                                                                |                                                                                                                                                   
ccTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAgg                                                                                                                                                 <  1:367764/149‑1 (MQ=255)
     ccccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCAAGCCCTGCCGCACTTGCGACAGGGctcg                                                                                                                                            <  1:245812/149‑3 (MQ=25)
      cccTTATTCCCGCCCCGGTGCGGACCATTATTCATGCCGTGCGTTCGTCGGTGGTGGCAGTGGCCCGCGATGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTTCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttc                                                                                                                                           <  2:1541434/149‑1 (MQ=37)
      cccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttc                                                                                                                                           <  2:1535332/149‑1 (MQ=255)
       ccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTATATTCTGCCGTATTATTCTCCGGGCCCTGTCGCTAGTGCGGCAGGTctttct                                                                                                                                          >  1:1522547/1‑149 (MQ=25)
        cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctt                                                                                                                                         <  1:614393/149‑1 (MQ=255)
        cTTAATCCCGCCCAGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGGGTTAGTGGCCCGCGCTGTCTGGGTTTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctt                                                                                                                                         <  2:904101/149‑1 (MQ=255)
               ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGt                                                                                                                                  >  2:1098971/1‑149 (MQ=255)
               ccGCCCCGGAGCGGACCATTATTCAGGCCGTGCGGTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGt                                                                                                                                  <  1:413485/149‑1 (MQ=255)
                    ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGGCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAc                                                                                                                             <  1:144027/149‑1 (MQ=255)
                     cGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCGTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTAGTTTTAcc                                                                                                                            >  1:1386565/1‑149 (MQ=255)
                     cGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCCAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                            >  1:1380962/1‑149 (MQ=255)
                     cGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                            <  2:76165/149‑1 (MQ=255)
                     cGGGGCGGTCCATTATTGAGGCCGGGCGTTGCTGGGTGGTGTCAGTGGCCCGCGCTGTCTGTGATTTCTTCTTGTGTGAGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                            <  1:663402/149‑1 (MQ=255)
                       gagCGGACCATTATACCGCCCGTGCGGTCCGCGGGGGTGGCAGGGGCCCGCGCGGTCTGGGATTGCTTCTTCTGAGGGTTTAATTCTGCCCTATTATTCTCCGCGCCCTGGCGCAAGTGCGGCAGGGCGTTCGTTATTTGTTTTACCCa                                                                                                                          <  2:1392790/147‑1 (MQ=255)
                           ggACCATTATTCAGGACGGGCGTTCCTCGGTGGTGTCTGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACt                                                                                                                      <  2:1513941/149‑1 (MQ=255)
                            gACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTg                                                                                                                     >  2:733078/1‑149 (MQ=255)
                             aCCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTga                                                                                                                    >  2:84992/1‑149 (MQ=255)
                              ccATTATTCAGGGCGTGCGTTCCTCGGTGGTGTCAGTGGCCCACGCTGTCTGGGATTTCTTGTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgag                                                                                                                   <  2:1551083/149‑1 (MQ=255)
                              ccATTAATCAGGCCGGGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCGGTCTGGGATTTCTTCTTCTGAGGGTTTAATTCTGCCCTATTCTGCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgag                                                                                                                   <  2:1125659/149‑1 (MQ=38)
                               cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGCGCCCTGTCGCAAGTGCGGCAGGGCGTTGTTTATTTGTTTTACCCATACTgagg                                                                                                                  >  2:154420/1‑149 (MQ=255)
                               cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCACTATTATTATCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACACCTACTgagg                                                                                                                  >  2:1389352/1‑149 (MQ=255)
                                 ttattCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgaggag                                                                                                                >  1:792870/1‑149 (MQ=255)
                                     gcAGGCCGTGCGCTCCTCGGTGGTGGCAGCGGCACTCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTg                                                                                                            <  2:175860/148‑1 (MQ=38)
                                      cAGGCCGTGCGTGCCTCGGGGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGc                                                                                                           <  1:720636/149‑1 (MQ=255)
                                       tgtccGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTTGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCt                                                                                                          <  1:1121365/146‑1 (MQ=255)
                                         gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGAGTTCTTCTTCTGAGTGTTTAATTCTGCGCGATTTTTCTCCGAGCCCTGGCGCAAGTGCGGCAGGGCTGTCTTTTTTTGTTTTACGCATCCTGAGGAGTCTGCttt                                                                                                        >  1:848441/1‑148 (MQ=37)
                                            gTGCGTTCCTCGGTGGTGGCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGa                                                                                                     <  2:1391625/149‑1 (MQ=255)
                                             tGCGTGCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                    <  2:1345767/149‑1 (MQ=255)
                                             ggCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGGCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                    <  2:381169/148‑1 (MQ=255)
                                               cGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCAGAGTGTTTAATTCTGCCCTATTAATCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAccc                                                                                                  <  2:170143/149‑1 (MQ=255)
                                                 ttCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                                >  1:67053/1‑149 (MQ=255)
                                                   ccTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCt                                                                                              >  1:38660/1‑149 (MQ=255)
                                                         gggggtCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGACTGTTAAATTCTGCCCTATTATTCTCCGAGCCCTGGCGCAAGGGCGGCAGGGCTTTCTTTATTTGTTTTCCCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTc                                                                                        <  2:505290/145‑1 (MQ=37)
                                                           ggcgcAGTGGCCCGCGCTGGGTGGGATTTCTTCTTCTGAGTGGTAAATTCTGCCCTATTTTTCTCCGAGCCCGCTCGCACGTGCTGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTcgc                                                                                      <  1:715347/145‑1 (MQ=38)
                                                              tCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCt                                                                                   >  2:478281/1‑149 (MQ=255)
                                                              tCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCt                                                                                   >  2:815060/1‑149 (MQ=255)
                                                                aGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCttt                                                                                 >  2:5970/1‑149 (MQ=255)
                                                                aGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGGGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCttt                                                                                 <  1:1129951/149‑1 (MQ=255)
                                                                 gTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                                >  2:1143362/1‑149 (MQ=255)
                                                                 gTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                                >  2:1234470/1‑149 (MQ=255)
                                                                   ggCCCGCGCTGGCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgc                                                                              <  1:1556180/149‑1 (MQ=255)
                                                                      ccGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcgcg                                                                           >  2:371266/1‑148 (MQ=255)
                                                                      ccGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCt                                                                           >  1:995757/1‑149 (MQ=255)
                                                                      ccGCGCTGTCTGGGATTTCTTCTTCTGAGTGAGAAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTGCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCt                                                                           <  1:1036588/149‑1 (MQ=255)
                                                                       cgcgCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCATATTATTCTCCGAGCCCTGGCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTTGCGCTTTGGCGCTg                                                                          <  1:1484451/149‑1 (MQ=255)
                                                                          gcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCCTTGGCGCTGCaa                                                                       <  1:587331/149‑1 (MQ=255)
                                                                            tGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAAt                                                                     <  1:84992/149‑1 (MQ=255)
                                                                             gTCTTGGTTTTCTTCTTTTGAGTGGTTAATTCTGCCCTTTAATTGTCCTATCCCTTTCTCAAGTGAGGCAGTGCTTTCTTTATTTGTTTTACCCATACTTAGGAGTCTTATTAAGACCCGTCTGGCTTCGCTCTTTGGCGCTGCATATc                                                                    <  2:1111080/149‑1 (MQ=255)
                                                                               cTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATACTCCGAGCCCTGTCTCAAGTGAGGCAGGGCTTTCTTTATTTGTTTTAAACATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTTGCGCTGCAAATCtt                                                                  >  1:1266614/1‑149 (MQ=38)
                                                                                tGGGATTTCTTCTTCTTAGTGTTTAATTCTGACCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                                 <  2:929428/149‑1 (MQ=255)
                                                                                tGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                                 >  1:1293112/1‑149 (MQ=255)
                                                                                tGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTATCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGTAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                                 >  2:625864/1‑149 (MQ=255)
                                                                                ggggATTGCTTCTTCTGAGGGTTTAATTCTGCCCTATTATTCTCCGATCCCTTGCGCAAGTGCGGCAGGGCTTTCTTTATTTGGTTTACCCTTACTGAGGAGTCTGCTTATGAGCCGTCTGGCTTGGCGCTTTGGCGCTGCAAATCTTa                                                                 <  1:1152057/148‑1 (MQ=255)
                                                                                 gggAGTTCTCCTTATGAGTGTTAAATTCTGCCCTATTATTCTCCGCGCCCTGTCGCACGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGCGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAt                                                                <  2:1418081/149‑1 (MQ=37)
                                                                                    aTTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTATCCGAGCCCTGTCGGAAGTGCGGCAGGGCTTTCTTTATTTGTTATACCCAGTCTGAGGAGTCTGCTTATGACCCCTCTGGCTTCGCGCTTTGTCGCTGCAAATCTTATTCg                                                             >  2:541663/1‑149 (MQ=38)
                                                                                     tttCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGc                                                            >  2:783623/1‑149 (MQ=255)
                                                                                         ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCAGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCAAACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTCTCGCTGCAAATCTTATTAGCCGTg                                                        >  1:350924/1‑149 (MQ=38)
                                                                                          tcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                       <  2:866675/149‑1 (MQ=255)
                                                                                                 aggctttAATTCTTCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGCCCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtccg                                                <  2:1522547/145‑1 (MQ=255)
                                                                                                     tttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTaa                                            >  1:332298/1‑149 (MQ=255)
                                                                                                          ttCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                       >  1:1142453/1‑149 (MQ=255)
                                                                                                               cccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAaga                                  >  1:337972/1‑149 (MQ=255)
                                                                                                                 cTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTCCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                                <  1:554467/149‑1 (MQ=255)
                                                                                                                  tattatTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGtt                               <  1:825725/149‑1 (MQ=255)
                                                                                                                  tattatTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGAGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGATAACCCGTGAAGAGtt                               >  2:128123/1‑149 (MQ=255)
                                                                                                                  tattatTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACACATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGtt                               >  2:361145/1‑149 (MQ=255)
                                                                                                                   attattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTACCCCGTGAAGAGTTa                              >  1:727286/1‑149 (MQ=255)
                                                                                                                   aatattCTCCGAGCCCTTCCGCACTTGCGGCAGGGCTTTCTTTATTTGTTTCACCCATTCTGTGGACTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTa                              <  2:1450408/147‑1 (MQ=21)
                                                                                                                    ttattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAt                             <  1:733078/149‑1 (MQ=255)
                                                                                                                      actcgccGAGCCCTGGCGCCAGTGCGGCAGGGCTGTCTTTATTTGTTTTACCCCTACTGAGGAGGCTGCTTATGCCCCGTCTGGCTTCGCGCGTTGGCGCTGCAAATCTTATTCGCCGTGACCGGCCGTAAACCCGTGAAGAGTTAttt                           <  2:1112107/144‑1 (MQ=255)
                                                                                                                       ttCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTc                          <  1:1206912/149‑1 (MQ=255)
                                                                                                                          tcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAATTGTTTTACCCATACAGAGGAGTCTGCTTATGACCCGTCTGGCTTCGGGCTTTGGCGATGCAAATCTTATTCGCCGTGCCCGTCCGTTAACCCGTGAAGAGGTATTTcgcg                       >  1:1340795/1‑149 (MQ=25)
                                                                                                                           gaaaGCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt                      >  2:412816/4‑149 (MQ=21)
                                                                                                                           ccGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt                      <  2:1275196/149‑1 (MQ=255)
                                                                                                                            cGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtg                     <  1:1480258/149‑1 (MQ=255)
                                                                                                                             aaGCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtga                    >  1:922971/2‑148 (MQ=255)
                                                                                                                              aGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGATTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt                   <  1:712799/149‑1 (MQ=255)
                                                                                                                              aGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTGGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt                   <  2:898470/149‑1 (MQ=255)
                                                                                                                                cccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTTGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGc                 >  2:795694/1‑149 (MQ=21)
                                                                                                                                 ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCAGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc                >  1:1109380/1‑149 (MQ=255)
                                                                                                                                 ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCATATCTTATTCGCGGTGCCCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc                >  1:1180338/1‑149 (MQ=21)
                                                                                                                                 ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc                >  1:184626/1‑149 (MQ=255)
                                                                                                                                  cTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc               >  2:255638/1‑149 (MQ=255)
                                                                                                                                               ggCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTg  <  1:478281/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTG  >  NC_000913/2768476‑2768767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: