| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,904,212 | G→A | 100% | intergenic (+838/+535) | ygcE → / ← queE | putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,904,212 | 0 | G | A | 98.8% | 317.9 / ‑6.5 | 128 | intergenic (+838/+535) | ygcE/queE | putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (62/57); minor base C (6/2); total (68/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 129 total aligned reads displayed.
TCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCC > NC_000913/2904066‑2904354 | tCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGACCACCTGTTTTGGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGACCACGGGCGCACGGAATACAACGCCGTGTATCTGCTCGGTTTATCCCCGCTGacg > 1:485686/1‑149 (MQ=255) aGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCAGTGTATCTGATCGGTTTATCCCCGCTGAcgcg > 2:1321847/1‑149 (MQ=255) tCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGa > 1:799396/1‑149 (MQ=255) ccGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGaa > 1:769896/1‑149 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac < 2:1266152/149‑1 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGATTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac < 1:221980/149‑1 (MQ=255) tATCCCCGCTGTCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTgg < 1:1068181/149‑1 (MQ=255) aTCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGc < 2:666303/149‑1 (MQ=255) cccGCTGGCGCGGGGAACACCTGTTTTCGCACATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAATGCCGTGTATATGCTCCGTTTATCCCCGCTGACGCGGGGCACACTGGctcg > 1:1436278/1‑148 (MQ=255) cGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGc < 2:108134/149‑1 (MQ=255) cGCTCGCGCGCGGAACCCCTGCGCGCGCAACTCTCTGGACTCTTCCTCTACTCTATATCCCCGCCGGAGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGTTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGc < 2:1010115/149‑1 (MQ=255) cTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCaa < 2:1076367/149‑1 (MQ=255) gggAACACCTGTTATCGCAAATCTATGGACTATTGCTATTCGGGTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAgcagca < 1:916923/149‑1 (MQ=255) gAACACCTGTGTTCGGAAAGCTATGGACTATTGCTTTTCGGTGTATCCCCGCTGGCGCGGGGAACTCGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAcc < 2:1435332/149‑1 (MQ=255) aaCACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAccc < 2:1294886/149‑1 (MQ=255) cacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAt < 2:957200/149‑1 (MQ=255) cacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACAATGGCTCTGCAACAGCAGCACCCAt < 1:382698/149‑1 (MQ=255) acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGAAGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATg > 2:1179980/1‑149 (MQ=255) cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGAc > 1:600761/1‑149 (MQ=255) ttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCTGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGt < 1:1254048/149‑1 (MQ=255) cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCg < 1:7742/149‑1 (MQ=255) cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCg < 1:103511/149‑1 (MQ=255) gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACCAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACATCCGCACGCATGACCAcggcgg > 1:370574/1‑149 (MQ=255) gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCgg > 1:249959/1‑149 (MQ=255) cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt > 2:590772/1‑149 (MQ=255) aaaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt > 1:888156/1‑149 (MQ=255) aaaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTAGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCCCCCATGACCACGTCGGtt > 2:497904/1‑149 (MQ=255) aaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGttt < 2:428387/149‑1 (MQ=255) aTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGAACCCAGTACAACGTCGGTTTa > 2:1383825/1‑149 (MQ=255) tCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt < 1:1033683/149‑1 (MQ=255) cTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATc > 1:232331/1‑149 (MQ=255) aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTGCAACAGCAGCACCCATGCCCGCGTCGGTTTATccc > 1:1140474/1‑149 (MQ=255) aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGAAAAAGCAGCACCCATGACCACGTCGGTTTATccc > 2:1170464/1‑149 (MQ=255) gACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAAAGCAGCACCCCTGCACACTTCGGTTTATCCCCGc > 2:440916/1‑149 (MQ=255) aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCt > 2:798061/1‑149 (MQ=255) aTTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGgc > 1:627993/1‑149 (MQ=255) aTTGCGATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTAGCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCTGCACCCATGACCACGTCGGTTTATCCCCGCTGgc < 1:509002/149‑1 (MQ=255) cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgg < 1:933184/149‑1 (MQ=255) cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTCACGCGGGGAACACTGGCTCTGCAACAGCCGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgg > 2:2490/1‑149 (MQ=255) cTATTCGGTTTATCCCCGCTGGCGCGGGGAAAACGGGCGCACGGAATACAAAGCCGTGTATCAGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAAAGCAGCACCCATGACCACGTCGGTTTATCCCCGCGGGCGCgg > 1:1386325/1‑149 (MQ=255) tATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg < 1:147963/149‑1 (MQ=255) aTTCGGTTTATCCCCGCTGGCGCGGGGAACACGGTCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgggg < 1:1143855/149‑1 (MQ=255) aTTCGGTTTATCCCCGCGGGCGCGGGGAACACGGGCGCACGGTATACACAGCCGTGTATCTGCACGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCCCGTCGGTTTATCCCCGCTGGCGCgggg < 1:1242981/149‑1 (MQ=255) tCGGGTTCTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTGTCTCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa < 1:1034544/149‑1 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTTTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAacac > 2:832414/1‑149 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCTACAGCAGCACCCATGACACCGTCGGTTTATACCCGCTGGCGCGGGGAactc > 2:1448346/1‑147 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCGGGCGCGGGGAacac > 1:492360/1‑149 (MQ=255) aTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGaaa < 1:1155720/149‑1 (MQ=255) ccccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATg > 1:1275184/1‑149 (MQ=255) cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGc > 1:1348471/1‑149 (MQ=255) ccGCTGGCGCGGGGAACACTGGCACAAGGAATTAAAAGCCATGAATCTGCTCGGTTTATCCCCACAGACGCGGGGAACACTGGCTCTGCAACAGCAGAACACAGTAACACGTAGGATTATCCACACTGGGTCGGGCAACACGAAATTCt > 2:121681/1‑149 (MQ=255) ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCt > 2:1491405/1‑149 (MQ=255) ggCGCGGTGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGa < 1:647214/149‑1 (MQ=255) gcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGTCTCTGCAACAGCAGCACCCATGACAACGTCGGTTTATACCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCg > 2:450499/1‑149 (MQ=255) gcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAAACATGACCACGACCGTTTATCCCCGCAGGCGCGGGCCACACGAAAAGCTGGTGAGcc > 1:1073902/1‑148 (MQ=255) cgGGGAACACGGGCGCACGGAATTCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGt < 1:169130/149‑1 (MQ=255) ggggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGtt < 1:1080351/149‑1 (MQ=255) gggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACCCGAAATGCGGGTGATCGTTa > 2:280181/1‑149 (MQ=255) ggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTaa > 1:431197/1‑149 (MQ=255) ggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTaa > 1:1403840/1‑149 (MQ=255) gAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACAACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTTGTGAGCGTTAAt > 1:483837/1‑149 (MQ=255) aaCACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCCTGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATg < 1:865164/149‑1 (MQ=255) aaCACGGGCGCACGGAATACAAAGCCCTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTCCCCCAGCAGCACCCAAGACCACGTCGGTTTATCCCCGCGGGCGCGGGGACCACGAAATGCTGGTGAGCGGGAATg > 2:1424141/1‑149 (MQ=255) aaagtgcgcACTGAATACAAAGCCGTGTATATGCGCGGTTTATCCCCGCTGTCGCGGGGAACACTTGCTCTGCCACAGCAGCCCCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc < 2:864807/144‑1 (MQ=255) acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg < 2:750557/149‑1 (MQ=255) acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGGCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg < 1:1109687/149‑1 (MQ=255) gggCGCACGGAATACAAAGCCGTGTATCTACTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAAAGCAGCACCCATGACCAAGTCTGTTTATCCCCGCTGGCCCGGGGAACAAGAAAAGCTGGTGCGAGTTAATGCCGCa > 2:53364/1‑149 (MQ=255) gcgcACGGAATACAAAGCCGTGTATCTTCTCGGTTTATACCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCaaa < 1:1321847/149‑1 (MQ=255) gcgcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCaaa < 1:1179980/149‑1 (MQ=255) gcgcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCaaa < 2:438558/149‑1 (MQ=255) gattACCAATCCTTGTATGTTCTCTGTTTAACCCGGCTGACGCGGTGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGTTTTATCCCCGCTGGCGGGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt < 1:529269/146‑1 (MQ=255) gAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt < 1:798061/149‑1 (MQ=255) gAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt > 2:1464874/1‑149 (MQ=255) gAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTAAATGCCGCAAACACAGGt < 1:1379897/149‑1 (MQ=255) aTACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGttt < 2:600761/149‑1 (MQ=255) cAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATc > 1:1557671/1‑149 (MQ=255) cAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATc > 1:1362035/1‑149 (MQ=255) aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc > 2:1293197/1‑149 (MQ=255) aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACAAAGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc > 2:310237/1‑149 (MQ=255) aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGAAGCGGGGCACACTGGCTCTGCCACAGCAGCACCCATGACCACGTCGGTTTATCCCAGCTGGAGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc > 1:890793/1‑149 (MQ=255) gCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATACCCg > 1:1539271/1‑149 (MQ=255) cGTGTATCTTCTCTGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAAAGCAGCACCGATGAACACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCt < 1:1465441/149‑1 (MQ=255) cGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCt > 2:245254/1‑149 (MQ=255) gtATCTGCTCGGTTTATCCCCTCTGACGCGTGGAACACTGGCTCTGCAACAGCAGAACCCATGACCACGTCTGTTTATCCCCGCTGGCTCGGGGATCACGAAATGCTGGTGAGCGTTTTTGCCTCAAACACAGGTTTATCCCCGCTGgc < 2:1395162/149‑1 (MQ=255) tATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgcg > 1:521270/1‑149 (MQ=255) tCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGcgcg > 2:754556/1‑149 (MQ=255) tCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGcgcg > 2:735305/1‑149 (MQ=255) cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGTGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgg < 2:133916/149‑1 (MQ=255) gCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCCCCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGCGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgggg > 1:1160360/1‑149 (MQ=255) cTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGa > 2:517578/1‑149 (MQ=255) cGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCCTCAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAac < 2:502304/149‑1 (MQ=255) ggTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAaca < 2:627993/149‑1 (MQ=255) gTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCAGTAGAGAGTTAATGACGCAAACAAAGTATTATACCCGCAGGCGCGGGGaaaaa > 2:909003/1‑146 (MQ=255) tttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTATTGCCGCAAACACCGGGTTATCCCCGCTGGCGCGGGGCAcgca > 1:520435/1‑149 (MQ=255) aTCCCCGCTTACGCGGGGAACACTTGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTAATCCCCGCTGGCGCGGGGAACACGACATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTa < 1:924262/149‑1 (MQ=255) ccccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAACCACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACg > 1:1470166/1‑149 (MQ=255) cccGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGc < 2:799396/149‑1 (MQ=255) ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcg > 2:43189/1‑148 (MQ=255) ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcc > 2:1051014/1‑149 (MQ=255) ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcc > 1:1535802/1‑149 (MQ=255) | TCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCC > NC_000913/2904066‑2904354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |