Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,321 C→A 6.5% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3210CA 6.5% 295.0 / 4.3 121noncoding (65/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (58/53);  new base A (2/7);  total (60/61)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976173‑2976467
                                                                                                                                                     |                                                                                                                                                  
caatcaatACCTCTGGGATCTGGAGTGTGGTATGCACCAGGCCACAGCGCCAGCGGGGAGAGGGAAACGGATGAAGGGAAAGGCAACGAGGTGGGTTGGTTTTCGGGGAGTGTGAAGGATTGAAGCCCTGAAAAAAAAACATGCGCGTc                                                                                                                                                    >  2:1065246/1‑149 (MQ=255)
   tcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgc                                                                                                                                                 <  1:177778/149‑1 (MQ=255)
     aatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCAgccc                                                                                                                                               >  2:342714/1‑147 (MQ=255)
        aCCTCTGGGATCTAGATTGTGGTCTGCACGACGACTCTTAGCCAGCGAGATAACGCAAAGGAATGAAGGGAAATGCAACGAGGAGTGTAAATTTTCGGTTAAAGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAgg                                                                                                                                            >  1:200065/1‑149 (MQ=255)
          ctctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                                                                          <  1:18158/149‑1 (MQ=255)
          ctctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                                                                          >  2:419255/1‑149 (MQ=255)
           tctGGTATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttt                                                                                                                                         <  1:1051685/149‑4 (MQ=255)
           tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCTGCGAGAAAACGCAAAGTAATGAAGGGAAATGCAACGAGGTGGGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                         <  1:706064/149‑1 (MQ=255)
                 aTCTTTATTGTGGTCTGCACGTCGACTCTTCGCCAGCTAGAATACGCAAAGGAATGAAGGGAAATGAAACGATGTGTGTAAATTTTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGAGCATCTGCGCAGGCTGGTGTaa                                                                                                                                   <  2:1480102/149‑1 (MQ=255)
                 aTCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTaa                                                                                                                                   <  1:414178/149‑1 (MQ=255)
                 aTCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTaa                                                                                                                                   >  1:947179/1‑149 (MQ=255)
                   attgtttgtTGTGTGCACGACGACTCTTCGCCAGCGCGAAAACGCAAAGGAAAGAAGTGAAATGCAACGAGGTGGGTAACTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAtt                                                                                                                                 <  1:1062080/148‑1 (MQ=255)
                      gATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                                                                              <  2:655397/149‑1 (MQ=255)
                        ttgttgGCTGCACGACGACTCTTCGCCAGCGAGAAACCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGGCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgt                                                                                                                            <  1:354209/149‑1 (MQ=255)
                        ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAACCGCAAAGGAATGAAGGGAAATGCAACGCGGTGGGTAAAGTGTCGGTTACTGTAACAGATTGATGCCCGGCAAAAAAAACCTGCCCATCTGCCCACGCTCGTGTAATTCCtgt                                                                                                                            >  2:1429465/1‑149 (MQ=255)
                          gaggTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                                                                          <  1:1300724/147‑1 (MQ=255)
                               cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGTAATGCAACGACGTGTGTAAATTGTCGGTTACTGTTACAGAATGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAc                                                                                                                     <  2:1384348/149‑1 (MQ=255)
                                  cACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTTTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCg                                                                                                                  >  1:30106/1‑149 (MQ=255)
                                    cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGATTGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGaa                                                                                                                <  1:877383/149‑1 (MQ=255)
                                       cgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGtt                                                                                                             <  1:1095171/149‑1 (MQ=255)
                                        gacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCTACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTg                                                                                                            >  1:332411/1‑149 (MQ=255)
                                        gacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTTCGCAGGCTGGTGTAATTCATGTGCTCAACCCTAAGTTg                                                                                                            >  1:1019766/1‑149 (MQ=255)
                                          ctctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                          <  2:562041/149‑1 (MQ=255)
                                          ctctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAAGGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                          <  2:1263629/149‑1 (MQ=255)
                                           tctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                         >  1:1430701/1‑149 (MQ=255)
                                                gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTAAACCCGAAGTTGACTTcccc                                                                                                    >  1:1441193/1‑149 (MQ=255)
                                                gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGAACGGCAAAAAAAACCTGCGCATATGCGCAGGCTGGAGTAATTCATGTGCTCAACCCGAAGTAGACTTCAcc                                                                                                    >  1:84886/1‑149 (MQ=255)
                                                gCCAGCGAGAAAACGCAAAGGAATCAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCaac                                                                                                    >  2:871485/1‑147 (MQ=255)
                                                 ccAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTTCTCAACCCGAAGTTGACTTCACCt                                                                                                   <  2:1207392/149‑1 (MQ=255)
                                                  cAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACGGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATGCATGTGCTCACCCCGAAGGTGACTTCACCTa                                                                                                  >  2:1059222/1‑149 (MQ=255)
                                                   aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGGTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTTTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTAt                                                                                                 >  1:507067/1‑149 (MQ=255)
                                                           aaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTTAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCCCAGGCTGGTGTAATTCATGTGCTCAACCCCAAGTTGACTTCACCTATCAATACct                                                                                         >  2:930734/1‑149 (MQ=255)
                                                             cGCAACCGACTGACGGCAACTGCACCGAGGTGTCTAAATTGTCGGGTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACctct                                                                                       <  2:1083708/149‑1 (MQ=255)
                                                              gCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTAAACCCGAAGTTGACTTCACCTATCAATACATCTg                                                                                      >  1:111010/1‑149 (MQ=255)
                                                                aaaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGTCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  1:217597/149‑1 (MQ=255)
                                                                   tgactGATGGGACATGCTACTCGTTATGTACATTGTCTGTTACTGTTACAGATTGATGAACTGAAAAAACAACCTCCGCTTCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGATGTTGACTTCACCTATCAATACCTCTGGGATc                                                                                 <  2:1180686/145‑1 (MQ=255)
                                                                    gAATGATGTGAAATTCAACGTGGTTTGTAAATTGTCGGGTACTGTTACAGATTGTTGAACTGCAAAAAAAACCTGCGCATATGAGCAGGCTGGTGTAATTCATGTGCTCAAGCCGAATTTGACTTCACCTATCAATACCTCTGGGATca                                                                                <  2:701712/149‑1 (MQ=255)
                                                                    gAATGAAGGGAAATTAAACGATGTGTGTAAATTGTCGGTTACTGTTAAAGATTTATGACCGGCAAAAAAATCCTGCGCATCTGCGCAGGCTGGTGTATTTCATGTGCTCTACCCGTAGTTGACTTCACCTATCAATACCTCTGGGATca                                                                                <  2:1430701/149‑1 (MQ=255)
                                                                    gAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTCCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATca                                                                                >  1:1157365/1‑149 (MQ=255)
                                                                      aTGAAGGGAAATGCTACGATGTGGCTTAATTGTCTTTTAATGATACAGATGGAGGCACGTCAAAAAAAAACGTTCGCCTCTGCGCAGGCTTGTGTAATTCTTGTGCGCAACCAGAATTTGACTTCACCTATCAATACCTCTGGGATcac                                                                               <  2:827265/149‑1 (MQ=255)
                                                                             gAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                       <  1:419255/149‑1 (MQ=255)
                                                                             gAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                       <  1:1393203/149‑1 (MQ=255)
                                                                               aaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCa                                                                     >  2:621375/1‑149 (MQ=255)
                                                                                aTGCAACGATGTGTTGAATTTGTCGGTTACTGTTACAGTTTCATGCCCTCCAAAAAACACCTGCGCAGCTGCTCATGCTGGTGTAAGTCTTCGTCTCAACCAGATTTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCaa                                                                    <  1:861506/149‑1 (MQ=255)
                                                                                 tGCAACGAGGTGTGTAAAATGTCGGGTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                   <  1:929646/149‑1 (MQ=255)
                                                                                    aaCGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTTTCAATAACACTGGGATCACCAATTTAGCAAACTg                                                                >  2:1559473/1‑149 (MQ=255)
                                                                                      cGAGGTGTGTAAATTGTCGGTTACTGTTACATATTGATTACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa                                                              <  1:898118/149‑1 (MQ=255)
                                                                                      cGAGCTGTGTAAATTGTCGGTTACTGTTACAGATTCATGACCTGAAAAAAAAACCTGCGCATCTGCGCAGGTTTGTGTAATTCATGTGCTCAACCCTACTATGACTTCATCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa                                                              <  2:200065/149‑1 (MQ=255)
                                                                                       taGGTGTGTATATTGTCTGTTACTGTTACAGATTGATGACCTTCAAAAAAAACCTGCGCATATGCGCAGGCTTGAGTAATTCATGTTCTCAACCCGATGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             <  1:1116792/148‑1 (MQ=255)
                                                                                       caGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             <  1:469674/148‑1 (MQ=255)
                                                                                         ggTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATAAATACCTCTGGGATCACCACTTTAGCAACCTGAAGcc                                                           >  1:559973/1‑149 (MQ=255)
                                                                                          gtgtgtAAATTGTCGGTTACTGTTACAGAGTGATGCCCGGCAAAAAAAACCTGCGCATCAGCGCCGGCTGGGGTAATTCATGTGCTCAACCCGAAGGAGACTTCCCCTATCAATACATCTGGGATAACCACTTTATCAACCTGAAGCCa                                                          >  2:839683/1‑149 (MQ=255)
                                                                                            gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACATGCGCATCTGCGCAGGCTGGTGTAATTCATGTTATAAACCCGATGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa                                                        <  2:460166/149‑1 (MQ=255)
                                                                                             tgtAAATTGTCTGTTACTGTTACAGATTGATGACAGTCAAAAAAAACCTGCGCAGCTGCTCAGGCTGGTGTAATTCAAGTGCTATAGCAGATTTTTACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                       <  2:241221/149‑1 (MQ=255)
                                                                                             tgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTGTAGCAACCTGAAGCCAAAc                                                       >  2:20254/1‑149 (MQ=255)
                                                                                             tgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGAAGGCTGGTGTAATTCATGTCCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                       >  1:1200549/1‑149 (MQ=255)
                                                                                             tgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACATGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                       <  2:518408/149‑1 (MQ=255)
                                                                                               tAAATTGTCGGTTACTGTTACAGATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATAAATACCTCTGGGATCCCCACTTTAGCAACCTGAAGCCAAAAGc                                                     >  2:1472948/1‑149 (MQ=255)
                                                                                                     ttaggTTTCTGTTAAAGATTGAAGAACGTAAAAAATAACCTCCGCATATGCTCTTGCTTGTGTAAGTCATGTGATAAAGTAGATTTTTATATCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAg                                               <  1:304564/146‑1 (MQ=255)
                                                                                                      tcttttACTTTTACAGATTGATGACCGGCTAAAAAAACTTTCGCATCTGCGCAGGATGGTGTAATTGATGTTATAAACAAGAATTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGc                                              <  1:852613/145‑1 (MQ=255)
                                                                                                      tCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCCCCACTGTAGCAACCTGAAGCCAAACGCCGCCAGc                                              >  2:669020/1‑149 (MQ=255)
                                                                                                       cGGTTACTGTTACAGATTGAGGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGAAATTCATGTGCGCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAACCGCCACCAGCg                                             <  1:781643/149‑1 (MQ=255)
                                                                                                         gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                           <  1:752401/149‑1 (MQ=255)
                                                                                                         gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                           >  1:905261/1‑149 (MQ=255)
                                                                                                          ttACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTTATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCGGGGATCACCACTTTAGCAACCTGAAGCCAAACGACACCAGCGGTc                                          >  2:1279056/1‑149 (MQ=255)
                                                                                                           tACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCa                                         >  2:1525687/1‑149 (MQ=255)
                                                                                                            aCTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTGTAGCAACCTGAAGCCAAACGCCACCCGCGGTCaa                                        >  2:1379746/1‑149 (MQ=255)
                                                                                                            aCTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCaa                                        >  1:725854/1‑149 (MQ=255)
                                                                                                                ttACAGATTGATGACCGGCAAAAAATACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCGCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    <  1:731214/149‑1 (MQ=255)
                                                                                                                ttACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    >  1:551624/1‑149 (MQ=255)
                                                                                                                  aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTTATTCATGTGCTCACCCCGAAGTTGCCTTCCCCTATCAATACCTCAGGGATCACCACGGTAGCAACCTGAAGCCAACCGCCACCCGCGGTCAATCGCaa                                  >  2:287623/1‑149 (MQ=255)
                                                                                                                  aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCCCCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                  >  2:1099297/1‑149 (MQ=255)
                                                                                                                  aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGCTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACGTATCAAAACCTCTGGGATCACCCCTATAGCAACCTGAAGCCAAGCGCCACCCGCGGTCAATCGCac                                  >  1:133085/1‑148 (MQ=255)
                                                                                                                          atgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTTATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGttt                          <  1:1496372/149‑1 (MQ=255)
                                                                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCCCCACTTTAGCAACCTGACGCCAAACGCCACCAGCGGTCAATCGCCACAGCGTTTCGCa                      >  2:1124536/1‑149 (MQ=255)
                                                                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                      >  1:1334906/1‑149 (MQ=255)
                                                                                                                               cGGCAAAAATAACCTGCTCATCTTCGCAGGCTGGTGTATTTCATGTGCTCACCCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCCGCGGTCAATCGCAACAGCGTTTCGCaa                     <  1:1483140/149‑1 (MQ=255)
                                                                                                                               cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaa                     >  1:795004/1‑149 (MQ=255)
                                                                                                                                ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTTGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    <  1:1472948/149‑1 (MQ=255)
                                                                                                                                ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    >  2:509005/1‑149 (MQ=255)
                                                                                                                                ggCAAAAAAAACCTGAGAATCTGCGGATGCTTGTGTAATTCATGTGTTCATCCCGTAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    <  2:84886/149‑1 (MQ=255)
                                                                                                                                 gCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   >  2:118746/1‑149 (MQ=255)
                                                                                                                                 gCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   >  2:552430/1‑149 (MQ=255)
                                                                                                                                    aaaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAgtg                 <  1:342714/149‑1 (MQ=255)
                                                                                                                                    aaaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTAAATCGCAACCGCGTTTCGCAAAgtg                 >  2:1371469/1‑149 (MQ=255)
                                                                                                                                       aaaaaCCTGCGCATTAGCGCAGGCTGGTGAAATTCATGTGCTCAACCCGAACTTTAGTTCACCAATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTaa              <  1:974124/149‑1 (MQ=255)
                                                                                                                                        aacaCCTGCGCATCTGCGCAGGCTTGAGTAATTCATGTGCGCAACCCGATGTTGAGTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAc             <  2:89296/146‑1 (MQ=255)
                                                                                                                                        aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAc             <  2:947179/149‑1 (MQ=255)
                                                                                                                                           gccTGCGCAGATGCGCAGGTTGGTGTAATTCATGTGCTCACCCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCaa          >  1:641269/2‑149 (MQ=255)
                                                                                                                                           aCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCaa          >  1:4932/1‑149 (MQ=255)
                                                                                                                                             cTGCGCATATGGGCTGGCTGGAGTAATTCATGTTCTCAAGCAGAAGTTGACGTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAg        <  2:86004/149‑1 (MQ=255)
                                                                                                                                              tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCCACAGCGTTTCGCAAAGTGTAACCAAAgg       >  1:61510/1‑149 (MQ=255)
                                                                                                                                                 gcATCTGCGCAGGCTGGTGTACTTCATGTGCTCACCCCGAATTTGACTTCACCTATCAATACCTCTGGGAGCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGttt    <  1:1065246/149‑1 (MQ=255)
                                                                                                                                                  cATCTGCTCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAACTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   <  1:930734/149‑1 (MQ=255)
                                                                                                                                                  cATCTGCGCAGGCTGGTTTAATTGATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   <  1:1059222/149‑1 (MQ=255)
                                                                                                                                                  cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   <  2:30106/149‑1 (MQ=255)
                                                                                                                                                  cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   <  1:272299/149‑1 (MQ=255)
                                                                                                                                                  cATATGCGCAGGCTGGTGTAATGCATGTGCTAAACCCGAATTTGACATCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTAAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   <  2:438401/149‑1 (MQ=255)
                                                                                                                                                   aTCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc  >  2:1327405/1‑149 (MQ=255)
                                                                                                                                                   aTCTGCGAAGGCTGGTGTAATTCATGTGCTAAACCCTAAGTAGACTACAACTATCAGTACCTCTGGGATCACCACTTTAGCAACCTGAATCCATACGACAAAAGAGTTCAATCGCAACTGCGATTCGATAAGTTTAAAGAAATGATTGc  >  2:1044907/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976173‑2976467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: