Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,066,310 A→T 5.2% H288Q (CAT→CAA ygfI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,066,3100AT 5.2% 308.3 / 4.1 106H288Q (CAT→CAAygfIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (46/54);  new base T (2/4);  total (48/58)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 107 total aligned reads displayed.

ATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAA  >  NC_000913/3066162‑3066455
                                                                                                                                                     |                                                                                                                                                 
aTCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAata                                                                                                                                                   >  2:463018/1‑149 (MQ=255)
  cGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGAATTACACCGTAATTTAATTGCATCCTGCTCGATGATGGGTTAATGACTCGTCCCGTCACTATATTCATCAATATg                                                                                                                                                 >  1:1104015/1‑149 (MQ=255)
  agggATTATCTGAATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATg                                                                                                                                                 <  1:638293/148‑1 (MQ=255)
   gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGc                                                                                                                                                >  2:1284125/1‑149 (MQ=255)
    ggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCt                                                                                                                                               <  1:334821/149‑1 (MQ=255)
    ggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCt                                                                                                                                               <  2:1201309/149‑1 (MQ=255)
     gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTg                                                                                                                                              <  1:953121/149‑1 (MQ=255)
       ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGaa                                                                                                                                            >  1:822839/1‑149 (MQ=255)
       ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCGGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGaa                                                                                                                                            <  1:561345/149‑1 (MQ=255)
         aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTAAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCATATTGCTGAAga                                                                                                                                          <  2:390771/149‑2 (MQ=255)
           cTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGGTAATGACTCGTCCCGGCACGATCTTCAGCATATTGCTGAAgatc                                                                                                                                        <  1:299652/149‑4 (MQ=255)
            tGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                       >  1:1218267/1‑149 (MQ=255)
            tGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                       <  1:1322172/149‑1 (MQ=255)
            tGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGAAATTTAATAGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                       <  1:1281226/149‑1 (MQ=255)
               aTCGCTATCTGGAAAATGCGCCTGGCGGACATATTCCCCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCggg                                                                                                                                    >  2:1218716/1‑147 (MQ=255)
               aTCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTg                                                                                                                                    <  2:567418/149‑1 (MQ=255)
                tCGCTATCTTGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                                                                   <  1:1162684/149‑1 (MQ=255)
                       cTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTATCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGATGCATCGGGGCGGtatt                                                                                                                            >  2:1372595/1‑148 (MQ=255)
                       cTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtata                                                                                                                            >  1:860065/1‑149 (MQ=255)
                        tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGTCGCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACgggacga                                                                                                                           <  2:541075/149‑6 (MQ=25)
                        tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                       <  1:376288/137‑1 (MQ=255)
                        tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATc                                                                                                                                       >  2:376288/1‑137 (MQ=255)
                        tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATaa                                                                                                                           >  2:755895/1‑149 (MQ=255)
                        tGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATaa                                                                                                                           >  2:21187/1‑149 (MQ=255)
                         ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTTAAGCATCGTGGCGGTATAAc                                                                                                                          >  2:1020408/1‑149 (MQ=255)
                         ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                                                          >  1:1499303/1‑149 (MQ=255)
                           aaaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                                                                                        <  1:102394/149‑1 (MQ=255)
                               tGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgatgat                                                                                                                    <  1:645010/149‑1 (MQ=255)
                                gcgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGAtt                                                                                                                   <  1:659604/149‑1 (MQ=255)
                                  gcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGCTCTTCAGCAATATGCTGAAGCAGCGTGGCGGTATAACGAAGATTTc                                                                                                                 >  2:716314/1‑149 (MQ=255)
                                    cTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGGCACGATCTTCAGCAATAAGCTGAAGCATCGTGGCGGTATAACGATGAttacag                                                                                                               >  2:1170439/1‑145 (MQ=255)
                                          gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCCGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAGCTTCAGCAATATTCTGAAGCATCGTGGCGGTATAACTATGATTTCTGATTGaa                                                                                                         <  1:1405740/149‑1 (MQ=255)
                                             atatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa                                                                                                      <  2:489962/149‑1 (MQ=255)
                                             atatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa                                                                                                      <  2:860065/149‑1 (MQ=255)
                                              tatTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                                                     <  1:1183518/149‑1 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                    <  2:1433640/149‑1 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                    <  2:916301/149‑1 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                    >  2:363830/1‑149 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                    >  1:791758/1‑149 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGAAACCGGCTCGATGCTTGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                                                                    <  2:1090873/149‑1 (MQ=255)
                                               atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTAAATTGCAACCGGCTCGATGCTGGGTTAATGACTCCTCCCGTCACGATCTTCAGCAATATGCAGAAGCATCGTGGCGGTATAACGAGGATATCTGATAGAATaaat                                                                                                    >  1:396800/1‑148 (MQ=255)
                                                ttCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                                                                   >  1:473906/1‑149 (MQ=255)
                                                    ccacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                                                                               >  2:376507/1‑149 (MQ=255)
                                                     cacGATCTTAGCCACGTCTTCGACTAACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCCTCCCGTCACTATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                                                              >  2:1189885/1‑149 (MQ=255)
                                                       cGATCTTAGCCACGTCTTCGTCTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                                                            <  2:79051/149‑1 (MQ=255)
                                                       cGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                                                            <  1:1020408/149‑1 (MQ=255)
                                                       cGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCAGCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                                                            >  2:692271/1‑149 (MQ=255)
                                                        gATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTg                                                                                           <  2:209188/149‑1 (MQ=255)
                                                         aTCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGc                                                                                          >  2:258292/1‑149 (MQ=255)
                                                          tCTTAGCGACGTCTTCGACTTACACCGTAATTTTATTGCAACCGGGGCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                                                                         <  1:691575/149‑1 (MQ=255)
                                                               gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATAc                                                                                    <  2:291840/149‑1 (MQ=255)
                                                                ccACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACa                                                                                   >  1:201839/1‑149 (MQ=255)
                                                                   cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCACGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCg                                                                                <  2:1087500/149‑1 (MQ=255)
                                                                     tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc                                                                              <  2:369587/149‑1 (MQ=255)
                                                                             ttACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg                                                                      <  2:387412/149‑1 (MQ=255)
                                                                             ttACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCCTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCAGCATATACAACGCACTGTGCgg                                                                      >  2:549037/1‑149 (MQ=255)
                                                                              tACACCGTAATTTAATTGAACCCGGCTCGATGCTGGGTTAATTCCTCGTCCCGACACGATCTTCAGCACTATCCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGt                                                                     <  2:860364/149‑1 (MQ=255)
                                                                               acacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                                                                    <  1:463018/149‑1 (MQ=255)
                                                                               acacCGTAATTTAATTGCAACCGGCTCGATGCTGGGGTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                                                                    <  1:988803/149‑1 (MQ=255)
                                                                                cacCGTAATTTAATTGCAACCGGCTCGATGCTTTGTAAATGACTCGTAACCACACGATCTACACCAATAACATGAAACATCGAGCCGATATAACGATCAATACTGATTTAAAAAACATATTCCTGCATATACATCCGCCTGAGCgcagc                                                                   >  2:508032/1‑145 (MQ=255)
                                                                                   cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                                                                >  1:104128/1‑149 (MQ=255)
                                                                                   cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                                                                >  1:541910/1‑149 (MQ=255)
                                                                                   cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                                                                >  1:1230617/1‑149 (MQ=255)
                                                                                    gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCa                                                               <  1:559521/149‑1 (MQ=255)
                                                                                    gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCGCGTCACGATGTTCAGCAATATGATGAAGCAGCGTGGCTGTATAACGATGATTTCTGATTTAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCa                                                               <  1:1139437/149‑1 (MQ=255)
                                                                                     taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaa                                                              >  1:601122/1‑149 (MQ=255)
                                                                                         ttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAatt                                                          >  2:208625/1‑148 (MQ=255)
                                                                                         ttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAata                                                          >  2:73216/1‑149 (MQ=255)
                                                                                                cAACAGGCTCGATGCTGGGTTAATGACTAGTCCCGTCACGATCTTCTGCAATATGATGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGTTATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCCGc                                                   <  2:1567485/149‑1 (MQ=255)
                                                                                                   ccGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGtt                                                <  1:750910/149‑1 (MQ=255)
                                                                                                      gCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGcc                                             <  2:1499303/149‑1 (MQ=255)
                                                                                                         cGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGa                                          <  2:1231827/149‑1 (MQ=255)
                                                                                                          gATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGGTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAt                                         <  2:450115/149‑1 (MQ=255)
                                                                                                           aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAtt                                        <  1:73216/149‑1 (MQ=255)
                                                                                                           aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTCATTGAATAAAGATATTCCTGCTAATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAtt                                        >  2:961462/1‑149 (MQ=255)
                                                                                                              cTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAc                                     <  1:124623/149‑1 (MQ=255)
                                                                                                               ggggTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCAcc                                    <  1:1170881/148‑1 (MQ=255)
                                                                                                                gggTTAATGACTCGTCCCGTCACGATCTTCAGCAATTTGCTTAAGCATCGTGGCGGTATAAGGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGGGCATCAAAATATTTCAGCGTTGCCGGATTCACCa                                   <  2:726427/149‑1 (MQ=255)
                                                                                                                 ggTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCCTCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAg                                  >  2:1104938/1‑149 (MQ=255)
                                                                                                                 ggTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAg                                  >  1:702085/1‑149 (MQ=255)
                                                                                                                  gTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGt                                 >  1:1357283/1‑149 (MQ=255)
                                                                                                                    tAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATTTTTCAGCGTTGCCGGATTCACCAGTCg                               >  1:1056421/1‑149 (MQ=255)
                                                                                                                      aTGACTCGTCCCGTCACGCTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATCTACATCGGCCTGTGCGGTGCCTCAAAATATTTCAGCGTTGCCGGCTTCACCAGTCGcc                             >  2:321336/1‑149 (MQ=255)
                                                                                                                       tGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa                            >  2:910131/1‑149 (MQ=255)
                                                                                                                        gACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAg                           <  2:1374603/149‑1 (MQ=255)
                                                                                                                             gTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCaaa                      >  1:1022103/1‑149 (MQ=255)
                                                                                                                                ccGTCACGATCTTCAGCAATATGCTGAAGCAGCGTGGCGGTATTACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGTTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATg                   >  1:348890/1‑149 (MQ=255)
                                                                                                                                  gccACGATGCTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                 >  2:1299620/3‑149 (MQ=38)
                                                                                                                                  gTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCg                 <  2:1357283/149‑1 (MQ=255)
                                                                                                                                   tCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCgg                <  2:822839/149‑1 (MQ=255)
                                                                                                                                    cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCggg               <  1:547773/149‑1 (MQ=255)
                                                                                                                                     aCGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGa              >  2:48236/1‑149 (MQ=255)
                                                                                                                                       gATCTTCATCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGACGGATTCACCATTCGCCAGGCAAAATGCGTGAta            >  2:855194/1‑148 (MQ=255)
                                                                                                                                       gATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATTTACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCTGGATc            >  1:1023844/1‑149 (MQ=255)
                                                                                                                                            ttCAGCATATTGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCg        >  2:1048349/1‑149 (MQ=255)
                                                                                                                                             tCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGt       <  1:516669/149‑1 (MQ=255)
                                                                                                                                              cAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTc      <  2:702085/149‑1 (MQ=255)
                                                                                                                                               aGCAATATGCTGAAGCATCGAGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATTTTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACGGTCa     >  1:272445/1‑149 (MQ=255)
                                                                                                                                                gCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAg    >  1:578675/1‑149 (MQ=255)
                                                                                                                                                  aaTATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa  <  1:1557432/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAA  >  NC_000913/3066162‑3066455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: