Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 216,081 C→T 5.1% intergenic (+102/+98) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913216,0810CT 5.1% 276.2 / 3.5 97intergenic (+102/+98)nlpE/yaeFlipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (52/40);  new base T (3/2);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTT  >  NC_000913/215936‑216229
                                                                                                                                                 |                                                                                                                                                    
ggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGCAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                                   >  2:1228673/1‑149 (MQ=255)
ggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCACCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACTCCGCATc                                                                                                                                                   >  1:1021620/1‑149 (MQ=255)
ggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                                   >  2:825945/1‑149 (MQ=255)
ggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                                   >  1:322379/1‑149 (MQ=255)
     ttttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                              <  1:570508/149‑1 (MQ=255)
       ttACCCCAACCATGATTGCAGTACTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAATGATATATATCAACATTTTCAATTTGCACGTTTTTTGTAGGCCAGATGCGGCGTAAACGCCTTATCCGGccta                                                                                                                                            <  1:161732/149‑4 (MQ=255)
          ccccAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGt                                                                                                                                         >  2:98524/1‑149 (MQ=255)
            ccATCCTGGATTGCAGTAGTTTGGGGCTGTAACCCTTCTTGAGTCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGc                                                                                                                                       <  2:150202/149‑1 (MQ=38)
                 cAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAc                                                                                                                                  <  1:443088/149‑1 (MQ=255)
                    gtttGCAGTTGTTTGGGGCAGTAACCCGTCTTGAGACAGTAACAAACGTAAAACAGGCCAGATGGATATATTTCATCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGcc                                                                                                                               <  2:711801/147‑1 (MQ=38)
                    gATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTGGTGCTCAACGcc                                                                                                                               >  2:709721/1‑149 (MQ=255)
                       tGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCCAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGa                                                                                                                            <  2:1204423/149‑1 (MQ=255)
                        gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGACACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGAt                                                                                                                           <  1:937490/149‑1 (MQ=255)
                        gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCTACATTTTGAATTTGCACTTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAGGTGCTCAACGACTGAt                                                                                                                           >  1:1383091/1‑149 (MQ=255)
                        gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGAt                                                                                                                           <  1:776670/149‑1 (MQ=255)
                           gtagtTTGGGGCAGTAACCCGTCTTGAGACAGCAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGTTTCTCAACGCCTGATGCg                                                                                                                        >  1:396436/1‑149 (MQ=255)
                              gtTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACg                                                                                                                     >  2:548122/1‑149 (MQ=255)
                               tttGGGGCAGTACCCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGc                                                                                                                    >  1:1499144/1‑149 (MQ=255)
                                   gggCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGgc                                                                                                                >  2:1152537/1‑149 (MQ=255)
                                          aaCCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCtt                                                                                                         <  1:122302/149‑1 (MQ=255)
                                            cccGTCTTGAGACAGAAACAAACTCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTTTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACTCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                       <  1:120198/149‑1 (MQ=255)
                                            cccGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                       <  1:357115/149‑1 (MQ=255)
                                             ccGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTAAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGAGTTTACGCCGCATCAGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGAGTCTTAta                                                                                                      >  1:47708/1‑148 (MQ=255)
                                              cGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                     <  1:441396/149‑1 (MQ=255)
                                               gTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                                                                    >  2:20830/1‑149 (MQ=255)
                                                 cTTGAGACAGAAACAAACGCAAAACAGGCCAGAATGAAATATTTCAACATTTTGAATTTGCACGTTTTTTTTAGGCCGGATAAGTCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGc                                                                                                  <  2:567601/149‑1 (MQ=255)
                                                 cTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGc                                                                                                  <  1:1226522/149‑1 (MQ=255)
                                                 cTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGc                                                                                                  <  1:847858/149‑1 (MQ=255)
                                                  ttGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGcc                                                                                                 <  1:1156386/149‑1 (MQ=255)
                                                  ttGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGcc                                                                                                 >  1:1506080/1‑149 (MQ=255)
                                                  ttGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGcc                                                                                                 <  1:548122/149‑1 (MQ=255)
                                                    gagaAAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGAGACGCTGGCGCGTCTTATCATGCCTa                                                                                               >  2:719865/1‑149 (MQ=255)
                                                     agaCAGAAACACACGCAAAACACGCCAGAAGCATATATTTCACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATACCGCGTTTACGCCGCATCCGGCAATGGTGCGCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACa                                                                                             <  1:490192/149‑1 (MQ=25)
                                                        cAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAc                                                                                           >  1:283459/1‑149 (MQ=255)
                                                         aGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTATGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGCTGCTCAACGCCTGATCCGACGCTGGAGCGTCTTATCATGCCTACAAcc                                                                                          >  1:481234/1‑149 (MQ=255)
                                                           aaacaaacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAcccc                                                                                        >  1:987646/1‑149 (MQ=255)
                                                            aacaaacCCAAAACAGGCCAGAACGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAccccc                                                                                       <  2:355643/149‑1 (MQ=255)
                                                              caaacGCAAAACAGGCCAGAATGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCt                                                                                     <  2:416854/149‑1 (MQ=255)
                                                              aaaacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTGTTTTGGAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCt                                                                                     <  2:1535429/148‑1 (MQ=255)
                                                               aaacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATCGTCCTCAACGCCTGATGCGACGCTGGCGCGTCATATCATGCATACAACCCCCCTc                                                                                    >  2:1086835/1‑149 (MQ=38)
                                                                 ccGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAt                                                                                  >  2:1100770/2‑149 (MQ=255)
                                                                 acGCAAAACAGGCCAGAAGGATATATATCAACATTTTGAATTTGCACGTTATTAGTAGGCCGGATAAGCCGTATACGCCGCATCCGGCAATGGTGATCAAAGCCTGATGCGACTCTGGCGCGTCTTATCATGCCTACAACCCCcatcat                                                                                  >  1:1147500/1‑149 (MQ=25)
                                                                   gCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTCCAACCCCCCTCATAc                                                                                >  2:747257/1‑149 (MQ=255)
                                                                   gCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATAc                                                                                >  2:741382/1‑149 (MQ=255)
                                                                   gCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATAc                                                                                >  1:179458/1‑149 (MQ=255)
                                                                   gCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCATTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATAc                                                                                <  1:220552/149‑1 (MQ=255)
                                                                     aaaaCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAGACCt                                                                              >  2:1487906/1‑149 (MQ=255)
                                                                      aaaCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATCCCtt                                                                             >  2:150536/1‑149 (MQ=255)
                                                                       aaCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTa                                                                            >  1:828433/1‑149 (MQ=255)
                                                                        aCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTaa                                                                           <  1:542695/149‑1 (MQ=255)
                                                                         ctggCCAGAAGGTTTTATTTCATCATTTTGAATTTGCACGTTTTTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAg                                                                          <  2:936556/147‑1 (MQ=255)
                                                                               aGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGAt                                                                    >  1:415216/1‑149 (MQ=255)
                                                                               aGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGAt                                                                    >  1:900308/1‑149 (MQ=255)
                                                                               aGAAGGATATATTTCAACATTNTGAATTTGCNNGTTTTTTGTAGNCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGAt                                                                    >  1:421100/1‑149 (MQ=255)
                                                                                gAAGGATATATTTCTACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCTATGGTTCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAGCCCCCTCATACCTTAAGGCGGATa                                                                   <  1:962383/149‑1 (MQ=255)
                                                                                   ggATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAgg                                                                <  1:566033/149‑1 (MQ=255)
                                                                                    gATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGc                                                               <  1:1345456/149‑1 (MQ=255)
                                                                                    gATATATTTCAACATTTAGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGc                                                               <  2:921916/149‑1 (MQ=255)
                                                                                     atatatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCa                                                              >  2:467649/1‑149 (MQ=255)
                                                                                       atatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCAtt                                                            <  1:1192549/149‑1 (MQ=255)
                                                                                         atTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTTCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACATTAAGGCGGATAAGGCATTTa                                                          >  1:1079365/1‑149 (MQ=255)
                                                                                          tttCAACATTTTGAATTTGCACGTTTTTTTTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTAc                                                         <  2:1142794/149‑1 (MQ=255)
                                                                                          tttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTAc                                                         >  1:664772/1‑149 (MQ=255)
                                                                                           ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATTAGGCATTTACt                                                        >  1:1164580/1‑149 (MQ=255)
                                                                                           ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACt                                                        >  2:786284/1‑149 (MQ=255)
                                                                                           ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACt                                                        >  2:555495/1‑149 (MQ=255)
                                                                                            tCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACTACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACtt                                                       >  1:982542/1‑149 (MQ=255)
                                                                                            ccAACATTTGAATTTGCACGTTTTAGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGGGAGCTCAACGCCTGATCCGACGCGGGCGCGGCGTATCATGCCTACACCCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACtgt                                                      <  2:1046057/148‑3 (MQ=25)
                                                                                             cAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACttt                                                      <  1:20830/149‑1 (MQ=255)
                                                                                               aCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTAt                                                    >  1:601790/1‑149 (MQ=255)
                                                                                                cATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGAATTTACTTAAta                                                   >  1:244179/1‑148 (MQ=255)
                                                                                                     tGAATTTGCACGTTTTTTGTAGGCCGGATAAGTCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCgg                                              <  2:1021620/149‑1 (MQ=255)
                                                                                                     tGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCgg                                              <  1:709721/149‑1 (MQ=255)
                                                                                                      tactttGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGTAATGGGGCTCAACGCCTGATGCGTCGCTGGCGCGTCTTATCATGCCTTCAACCGCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCggg                                             <  2:1447548/146‑1 (MQ=37)
                                                                                                              aCGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACca                                     >  2:329255/1‑149 (MQ=255)
                                                                                                              aCGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACca                                     >  1:52246/1‑149 (MQ=255)
                                                                                                                gTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATCCCTTACGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaac                                   <  2:1429680/149‑1 (MQ=255)
                                                                                                                 ttttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCCTACCTTAAGGCGGATAAGGCATTAACTTTATCACCGGGTCAAACcaacc                                  >  2:500671/1‑148 (MQ=255)
                                                                                                                 ttttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAACCcaaca                                  >  1:1012068/1‑149 (MQ=255)
                                                                                                                 ttttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaaca                                  >  2:1276296/1‑149 (MQ=255)
                                                                                                                  tttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaacaa                                 >  1:172900/1‑149 (MQ=255)
                                                                                                                  tttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGCGCTCAACGCCTGAAGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaacaa                                 <  2:662610/149‑1 (MQ=255)
                                                                                                                   ttttGTAGGCAGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCATGATCCGACGCTGGAGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACaaa                                >  1:386514/1‑149 (MQ=255)
                                                                                                                        tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAc                           >  1:651673/1‑149 (MQ=255)
                                                                                                                         ttgCCGGATGCGGCGTAAACGCCTTATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAACCCACCCAAGCGAcc                          >  2:1142239/3‑149 (MQ=37)
                                                                                                                         ttgCCGGATGCGGCGTAAACGCCTTATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTAAAACCAACAAAGCGccc                          >  2:509441/3‑149 (MQ=38)
                                                                                                                             cGGATGCGGCGTAAACGCCTTATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc                      >  1:1522100/1‑149 (MQ=38)
                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc                      >  2:369485/1‑149 (MQ=255)
                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCtt                    <  2:361870/149‑1 (MQ=255)
                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCtt                    >  2:491266/1‑149 (MQ=255)
                                                                                                                                 tAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGa                  <  1:188045/149‑1 (MQ=255)
                                                                                                                                 tAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGAGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGa                  <  2:599554/149‑1 (MQ=255)
                                                                                                                                   aGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGata                >  1:522760/1‑149 (MQ=255)
                                                                                                                                          tACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGCCCCGCCTTGATATAAATcc         >  1:293862/1‑149 (MQ=255)
                                                                                                                                              ccgcATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAgg     <  1:747257/149‑1 (MQ=255)
                                                                                                                                              ccgcATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAgg     <  1:741382/149‑1 (MQ=255)
                                                                                                                                               cgcATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGc    >  1:591140/1‑149 (MQ=255)
                                                                                                                                               cgcATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGc    >  1:548226/1‑149 (MQ=255)
                                                                                                                                               cgcATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCCCCTCATACCGTAAGGCGGATAAGGCATTTAATTTATCACCGGGTCAAACCAACAAAGCGACCCGCCGTGATATTAATCCCCggg    >  2:896024/1‑148 (MQ=38)
                                                                                                                                                 cATCCGGAAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACGTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCtt  <  2:722019/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTT  >  NC_000913/215936‑216229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: