Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,139,639 T→G 5.1% intergenic (+46/+77) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,139,6390TG 5.1% 327.6 / 2.9 117intergenic (+46/+77)yghU/hybGputative S‑transferase/hydrogenase 2 accessory protein
Reads supporting (aligned to +/- strand):  ref base T (69/41);  new base G (4/3);  total (73/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGAT  >  NC_000913/3139492‑3139787
                                                                                                                                                   |                                                                                                                                                    
cGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTc                                                                                                                                                     >  2:706088/1‑149 (MQ=255)
cGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGGTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTc                                                                                                                                                     >  1:1489433/1‑149 (MQ=255)
    ttGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                                                                                                 >  2:253515/1‑149 (MQ=255)
       ttAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                              >  2:1439240/1‑149 (MQ=255)
       ttAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGGCAGGGGTAAGTGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                              >  2:285470/1‑149 (MQ=255)
         aaCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                                                            >  2:889245/1‑149 (MQ=255)
           ccGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                          >  1:478101/1‑149 (MQ=255)
               aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                      >  1:495403/1‑149 (MQ=255)
                 cAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCGTGCCGCCAGTGATTTCGAGACGGCTACGGAAGAGACGGGTCAGGGGGATGGGGTGGGGTTGGGCGCAGCACGCCGTCCCGGCCGGAGAAGGCGGTCCCGCCGCCTCGGGCaag                                                                                                                                    >  2:1050395/1‑148 (MQ=255)
                 cAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                                                                                                    >  2:569113/1‑149 (MQ=255)
                 cAACGGACCGCTCACTGAGCAGTTGCATGCGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGCTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATGCGGCGTGAACGCCTTATCCGGcctg                                                                                                                                    <  1:447987/149‑4 (MQ=255)
                  aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCTAGTGATTTCGAGTCGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATc                                                                                                                                   <  2:1114320/149‑1 (MQ=255)
                  aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCATc                                                                                                                                   >  2:147610/1‑149 (MQ=255)
                   aCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCg                                                                                                                                  >  1:433003/1‑149 (MQ=255)
                        ccGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGAAAGGGTTGGTGTTCGTCGCAGCAAGCCTTCCAGGCCGGATAAGGCGTTAACTCCGCATCCGGCAAACGTGcac                                                                                                                             >  1:890893/1‑149 (MQ=255)
                             gAATGAGCAGTTGCATGAGCGCCATGCCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAATCGTGCACAATGc                                                                                                                        >  2:1560211/1‑149 (MQ=255)
                              aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                                                                                       <  1:1233246/149‑1 (MQ=255)
                                          cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCt                                                                                                           >  1:758802/1‑149 (MQ=255)
                                               gcgcCATGACGCGAGTGCTGTCGAGACGAATACGGAAGCTCACCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGCGTGCACAATGCCTGATGCGACGCTGCcgc                                                                                                      <  1:867411/149‑1 (MQ=37)
                                               gcgcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCcgc                                                                                                      <  1:569113/149‑1 (MQ=255)
                                                  ccATGAAGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGGCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTc                                                                                                   <  2:37332/149‑1 (MQ=255)
                                                   cATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCt                                                                                                  >  1:368835/1‑149 (MQ=255)
                                                   cATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCt                                                                                                  >  1:374670/1‑149 (MQ=255)
                                                    aTGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                 >  1:736944/1‑149 (MQ=255)
                                                      gACGCCAGTGATTTCGAGACGAATACGGATGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAt                                                                                               <  2:1342357/149‑1 (MQ=255)
                                                      gACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAt                                                                                               <  2:642026/149‑1 (MQ=255)
                                                         gCCAGTGATTTCGAGACGAATACGGAAGATCAGCGTCAGGGGTAATGGCTGGTGTTCGGCGCAGCAAGCCATCCTGGCCGGATAAGGAGTTCACGCCGCATCCGTCAATCGTGCTCAATGCCTGATAAGACGCGGCAGCGTCGCATCAg                                                                                            <  1:307237/149‑1 (MQ=255)
                                                         gCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAg                                                                                            >  1:1156930/1‑149 (MQ=255)
                                                         gCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAg                                                                                            >  2:1157591/1‑149 (MQ=255)
                                                         gCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGCTTGGTGATCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGAGCACAATGCCTGATGCGACGCAGCCGCGTCTTAGCAg                                                                                            >  2:426457/1‑149 (MQ=38)
                                                           cAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                                                                                          >  1:593696/1‑149 (MQ=255)
                                                            aGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGCCAATCGTGCACAATGCATGATGCGACGCTGCCGCGTCTTATCAGGcc                                                                                         >  1:1261376/1‑149 (MQ=255)
                                                                aTTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTAGTCGCAGCAAGCCATCCAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACg                                                                                     >  2:754572/1‑149 (MQ=255)
                                                                 tttCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAGGCCATCCAGGCCGGATAAGGCGTTCACGACGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGa                                                                                    >  1:284667/1‑149 (MQ=255)
                                                                 tttCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGa                                                                                    >  1:169367/1‑149 (MQ=255)
                                                                  ttCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaa                                                                                   >  2:249081/1‑149 (MQ=255)
                                                                   tCGAGTCGATTACGGTATATAAGCTTCTGGGGTAAGGGTTGGAGTTCGGCCCAGCTAGCCATCCTGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACTCTGCATGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                  <  1:931594/149‑1 (MQ=21)
                                                                         cGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAat                                                                            <  1:1183496/149‑1 (MQ=255)
                                                                          gAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatc                                                                           >  1:1105787/1‑149 (MQ=255)
                                                                          gAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTAGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCATACGAAAGCTAatc                                                                           >  2:1109019/1‑149 (MQ=255)
                                                                               cGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAc                                                                      <  1:1439240/149‑1 (MQ=255)
                                                                               cGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAc                                                                      >  1:535854/1‑149 (MQ=255)
                                                                                 gAAGATAAGCGTAAGGGGTAAGGGTTGGTGGTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc                                                                    <  2:1436742/149‑1 (MQ=255)
                                                                                  aaGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgcg                                                                   >  1:544020/1‑149 (MQ=255)
                                                                                   aGATAAGCGGCAGGGGTAAGGGTTGGGTGTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTGCACGCCGCATCCGGCAATCGTTCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGTCCTACGAAAGCTAATCATCACgcgc                                                                  <  2:1402914/149‑1 (MQ=37)
                                                                                     ttatgCGTGAGGGGGAAGGGTTGGTGGTCGTCGCTGCAAGCCATACTGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTCGTGCACAATCCCTGCAGCGACGCTGCCTGGTCTAATCAGGCGTACGAAAGCTTATCATCACGCGCtt                                                                <  2:699377/145‑2 (MQ=25)
                                                                                     aTAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg                                                                >  1:357448/1‑149 (MQ=255)
                                                                                       aaGCGTCAGGGCAAAGGGTTGGAGTTCGTCGCAGCAAGCCAACCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCCCAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt                                                              >  2:1417472/1‑149 (MQ=37)
                                                                                        agctgcAGGGGTTTGGGTTGGTGTTCGTCGCAGCAAGCCTTCCAGGCCGGTTAAGGCGTTCTCGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTa                                                             <  2:1489433/144‑1 (MQ=37)
                                                                                         gCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa                                                            >  1:696896/1‑149 (MQ=255)
                                                                                           gTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg                                                          >  1:578433/1‑149 (MQ=255)
                                                                                            tCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt                                                         >  1:458289/1‑149 (MQ=255)
                                                                                             cAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTc                                                        <  1:706088/149‑1 (MQ=255)
                                                                                               ggggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGt                                                      >  1:194791/1‑149 (MQ=255)
                                                                                               ggggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTAAGAAAGATAATCATCACGCGCTGGTAATGTCGt                                                      >  1:1057306/1‑149 (MQ=255)
                                                                                                gggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTa                                                     >  2:1021444/1‑149 (MQ=255)
                                                                                                gggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTa                                                     >  1:1151148/1‑149 (MQ=255)
                                                                                                  ggaaGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAAt                                                   <  1:539304/147‑1 (MQ=255)
                                                                                                  gTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAAt                                                   <  1:138674/149‑1 (MQ=255)
                                                                                                   tAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATc                                                  >  2:614378/1‑149 (MQ=255)
                                                                                                        ggtGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                             <  2:1439418/147‑1 (MQ=255)
                                                                                                        gTTGGTGTTCGTCGCAGCTAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                             <  2:444105/149‑1 (MQ=255)
                                                                                                        gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                             <  2:31804/149‑1 (MQ=255)
                                                                                                            gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGgcg                                         >  1:82701/1‑149 (MQ=255)
                                                                                                               ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAg                                      >  1:704092/1‑149 (MQ=255)
                                                                                                               ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAg                                      >  1:658835/1‑149 (MQ=255)
                                                                                                                  gtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAGCAGGCCTACGAAAGCTAATCATCACGCGCTGGTATTGTCGTACTCCATTTGGCGCAGTGc                                   >  2:871518/1‑149 (MQ=255)
                                                                                                                   tcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCCTCACGCGCTGGTAATGTCGTACTCCATTTGGCGCAGTGCg                                  >  1:1249731/1‑149 (MQ=255)
                                                                                                                       agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTa                              <  1:253515/149‑1 (MQ=255)
                                                                                                                       agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAAAGTCGTAATCCATTTGGCGCAGTGCGTCTa                              >  1:863783/1‑149 (MQ=255)
                                                                                                                       agcaAGCCATCCAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTa                              <  2:919742/149‑1 (MQ=255)
                                                                                                                         caAGCCATCCAGGCCGGATAATGCGTTCACGCCGCATCCGGCAATCGTGACCAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAt                            <  2:1279392/149‑1 (MQ=255)
                                                                                                                           aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgt                          >  2:166910/1‑149 (MQ=255)
                                                                                                                           aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgt                          <  1:160772/149‑1 (MQ=255)
                                                                                                                           aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTACCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgt                          >  2:425650/1‑149 (MQ=255)
                                                                                                                            gCCATCCAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGACGCGTATTTTCAGGCCTACGAAAGCTAATCATCACGCGCTGGTTTTGTCGTATTCCATTTGGCGCAGTGCGTCTATtgtg                         >  1:199796/1‑149 (MQ=37)
                                                                                                                            gCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgtg                         <  2:357448/149‑1 (MQ=255)
                                                                                                                             ccctCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCAGCACGCGCTGGGAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtgg                        >  1:1343729/4‑149 (MQ=255)
                                                                                                                             ccATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtgg                        <  2:820973/149‑1 (MQ=255)
                                                                                                                              cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggc                       >  1:1136197/1‑149 (MQ=255)
                                                                                                                              cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCGTATCAGGCCTACGAAAGCTAATCATCCCGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggg                       >  1:152937/1‑148 (MQ=255)
                                                                                                                               aTCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCCCGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                      >  2:513400/1‑149 (MQ=255)
                                                                                                                                tCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTAGACGCACTGCGCCAAAtggattac                     <  2:544020/149‑7 (MQ=25)
                                                                                                                                  cAGGCCGGATAAGGCGTTCACGCCGGATCCGGCAATCGTGCACAATGCCGGAAGCGACGCTGCCGCGTCTTATCAGGCCTACGAACGCTAATCATCACTCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttg                   <  1:426457/149‑1 (MQ=37)
                                                                                                                                   ttgCCGGATGCGGCGTGAACGCCTTATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTATTGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttgg                  >  1:1162530/3‑149 (MQ=38)
                                                                                                                                   ttgCCGGATGCGGCGTGAACGCCTTATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttgg                  >  2:1346667/3‑149 (MQ=38)
                                                                                                                                   aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTACTCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttgg                  >  1:1409422/1‑149 (MQ=255)
                                                                                                                                    ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATTTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggc                 <  1:774123/149‑1 (MQ=255)
                                                                                                                                     gCCGGATATGGCGTTCACGCCGCTTCCGGCAATCGTGCACAATGCCTGATGCGTCGCTGCAGAGTCTTATCAGGCGTACTAAAGCTAATCATCACGCGCTGGTAATGTCGTATTCCATTTTGCGCAGTGCGTCTAATGtggctttggct                <  1:356263/149‑1 (MQ=255)
                                                                                                                                      ccGGATGCGGCGTGAACGCCTTATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGGAATGTCGTAATCCATTTGGCGCGGTGGGGCTAATGtggctttggctt               >  2:1149039/1‑149 (MQ=37)
                                                                                                                                      ccGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt               >  2:153271/1‑149 (MQ=255)
                                                                                                                                         gATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCAGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCTCTGGTAATGTCGTAAACCATTTGGCGCAGTGCGTCAAATGTGGCTTTGGCTTCAt            >  2:835630/1‑149 (MQ=255)
                                                                                                                                           tacgtcGTTCACGCGGGATCCTGCAATCTTGCACAATGGCGGTTGCTACGCTGCCGCGTCTTATCAGGCCTACGATAGCTAATCATCTCGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCt          <  1:1073666/144‑1 (MQ=255)
                                                                                                                                           tAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCt          >  2:173575/1‑149 (MQ=255)
                                                                                                                                            aaGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCtt         <  2:578433/149‑1 (MQ=255)
                                                                                                                                             aGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtc        >  2:294347/1‑149 (MQ=255)
                                                                                                                                               gCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcgt      >  2:428659/1‑149 (MQ=255)
                                                                                                                                               gCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcgt      >  2:1196695/1‑149 (MQ=255)
                                                                                                                                                cGTTCACGCCGCATCCGGCAATCGTGCACACTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcgtc     <  2:535854/149‑1 (MQ=255)
                                                                                                                                                   tCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCgat  <  2:704092/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGAT  >  NC_000913/3139492‑3139787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: