Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,234,507 A→T 5.9% intergenic (‑52/+232) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,5070AT 5.9% 350.1 / 5.2 125intergenic (‑52/+232)higB/rlmGmRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (60/56);  new base T (5/4);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTG  >  NC_000913/3234359‑3234654
                                                                                                                                                    |                                                                                                                                                   
ccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa                                                                                                                                                     <  1:155034/149‑1 (MQ=255)
   tAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTg                                                                                                                                                  <  1:1520665/149‑1 (MQ=255)
   tAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTg                                                                                                                                                  <  1:836587/149‑1 (MQ=255)
   tAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTg                                                                                                                                                  <  1:984996/149‑1 (MQ=255)
     gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                <  1:553595/149‑1 (MQ=255)
     gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCCTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                <  1:1285973/149‑1 (MQ=255)
       aaTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCaa                                                                                                                                              >  2:723205/1‑149 (MQ=255)
       aaTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCaa                                                                                                                                              >  1:1475760/1‑149 (MQ=255)
          cgagtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATCTGGCAACgg                                                                                                                                           <  1:633392/146‑1 (MQ=255)
            tgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                         <  2:495563/149‑1 (MQ=255)
             gtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATg                                                                                                                                        >  2:648447/1‑149 (MQ=255)
               accccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCACTGCTTTTTGACTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACCTCCGTTGCCAGATTGGCAACGGATGaa                                                                                                                                      <  2:848755/148‑1 (MQ=255)
                ccccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAt                                                                                                                                     <  2:621717/149‑1 (MQ=255)
                ccccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAt                                                                                                                                     <  1:827461/149‑1 (MQ=255)
                ccccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAt                                                                                                                                     >  1:1153781/1‑149 (MQ=255)
                 cccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAtt                                                                                                                                    >  1:1275717/1‑149 (MQ=255)
                 cccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAtt                                                                                                                                    >  1:1570545/1‑149 (MQ=255)
                  cccgagCCATCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGGGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt                                                                                                                                   <  2:336044/146‑1 (MQ=255)
                  ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt                                                                                                                                   >  1:1399155/1‑149 (MQ=255)
                      tgCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGTTTGGCAACGGATGAATTTATCt                                                                                                                               <  2:1333204/148‑1 (MQ=255)
                        ccagcaACTACGTTTTATGTTTCGGGTATTTTTCCGAAGGATCTTTCAATGCTTTTTGAGTTATCAGGTGGATTCTTCTTCTCTTCCGTTGAGATTTTGGCAATATGATATCATCGGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                             <  2:716425/149‑1 (MQ=255)
                        ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                             >  2:977461/1‑149 (MQ=255)
                        ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                             <  1:235046/149‑1 (MQ=255)
                         caccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtg                                                                                                                            >  1:186070/1‑149 (MQ=255)
                          accaACTCCGTTTTATGGTGCGGGTATTTTTCCTCAGCATCTTTCAATGCTTTTTGATTTCTCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtgt                                                                                                                           <  2:258906/149‑1 (MQ=255)
                           ccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtgtg                                                                                                                          >  2:930448/1‑149 (MQ=255)
                             aactaagTTTTATTTTGCGTGTATTTTTACGCATCATATTTCAATGATTTTTGAGTTATCAGGTGCTTTCTTCATCACGTCAGTTGACAAATTGGCAATATGATAACATCCGCTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAc                                                                                                                        <  1:965376/143‑1 (MQ=255)
                             aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAc                                                                                                                        <  1:1041031/149‑1 (MQ=255)
                             aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAc                                                                                                                        >  1:340129/1‑149 (MQ=255)
                                tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGga                                                                                                                     >  1:79664/1‑149 (MQ=255)
                                       tATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATaa                                                                                                              <  2:1432555/149‑1 (MQ=255)
                                          gTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACg                                                                                                           >  2:1416466/1‑149 (MQ=255)
                                               gggTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaa                                                                                                      >  2:678702/1‑149 (MQ=255)
                                                 gTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCGAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaa                                                                                                    >  2:502980/1‑149 (MQ=255)
                                                  tATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaaa                                                                                                   <  2:1181287/149‑1 (MQ=255)
                                                         ccGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTaaa                                                                                            >  2:267698/1‑149 (MQ=255)
                                                          cGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATACGTTGCCAGATTGGCAACGGATGAAATTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAAc                                                                                           >  1:22521/1‑149 (MQ=255)
                                                           gcagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACa                                                                                          >  2:671058/1‑149 (MQ=255)
                                                           gcagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGAATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACa                                                                                          >  1:850705/1‑149 (MQ=255)
                                                            cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGGTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACaa                                                                                         >  2:1315877/1‑149 (MQ=255)
                                                                 tCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGAAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt                                                                                   <  1:1533762/149‑1 (MQ=255)
                                                                  cTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt                                                                                   <  1:1222065/149‑1 (MQ=255)
                                                                   tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATg                                                                                  >  2:1511842/1‑149 (MQ=255)
                                                                    ttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCTAAGCCAGATTGGCAAAGGATGAATTTATCTGTGTGACGGAAGAAAAAACTTAGAAAAACGCTAAACAACAGAATGt                                                                                 >  1:1409907/1‑149 (MQ=255)
                                                                    ttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTTCCAGATTGGCAACGGATTAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt                                                                                 <  2:842268/149‑1 (MQ=255)
                                                                    ttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt                                                                                 <  1:299011/149‑1 (MQ=255)
                                                                     tCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTa                                                                                <  1:648447/149‑1 (MQ=255)
                                                                     tCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTa                                                                                >  1:469087/1‑149 (MQ=255)
                                                                       aaTGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                              >  1:672948/1‑149 (MQ=255)
                                                                          gCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAATAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGa                                                                           <  2:1384605/149‑1 (MQ=255)
                                                                               ttGAGTTATCAGGTGCAATCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTg                                                                      <  2:419896/149‑1 (MQ=255)
                                                                                 gAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAAACGATGGTGAc                                                                    >  1:942986/1‑149 (MQ=255)
                                                                                     tATCAGGTGCATTCTTCATCACGTCCGTAGACAAATTGGCAATAAGATAACATCCGTTGCCAGAATGGCAACGGATGAATTTATCTGTGTTACGTAAGAATAAACGAAGAAAAAAGCTAAACAAGAGAATGTAGACTATGTTGACAggg                                                                >  1:1511238/1‑149 (MQ=255)
                                                                                       tCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATATACGGTGATAAACGCTAAACAAGAGAATGTTGACGATGGTGACAGGGAt                                                              >  1:1060100/1‑149 (MQ=255)
                                                                                         aGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttt                                                            >  1:1043465/1‑149 (MQ=255)
                                                                                          ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAGGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttt                                                           >  1:290149/1‑149 (MQ=255)
                                                                                           gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATATCATCCGTTGCGAGTTTGGCTACGGATGTAATTATCTGTGTGACGGATGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGAAAGGGAttttt                                                          <  2:127375/149‑1 (MQ=255)
                                                                                              cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt                                                       <  2:41756/149‑1 (MQ=255)
                                                                                                  attcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                   <  1:991768/148‑1 (MQ=255)
                                                                                                   ttcATCACGTCCGTTTACAAATTTGCAATAAGATAACATCCGTTGCCATTTTGGCAACGGATGAATTTATCTTTGTGACGGTATAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt                                                  <  1:977461/149‑1 (MQ=255)
                                                                                                   ttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt                                                  >  2:35407/1‑149 (MQ=255)
                                                                                                    tcatcaCGTCCGTTGACAAATTGGCAATATGATAACATCCTTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATg                                                 <  2:1457062/149‑1 (MQ=255)
                                                                                                     catcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATgg                                                <  2:1275717/149‑1 (MQ=255)
                                                                                                       tcaCGTCAGTTGAGAAATTTGCAATATGATAACATCCGTTGCCAGATTGGAAACGGATGAATTTATCTTTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGaa                                              <  2:792453/149‑1 (MQ=255)
                                                                                                         aCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgc                                            >  2:590486/1‑149 (MQ=255)
                                                                                                         aCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAGAGGAAACCGATGAATTTATCTGTGTGCCGGAAGAATAAACGTAGATAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGCATATTTTGTTTTATGGAAgc                                            >  2:1416284/1‑149 (MQ=255)
                                                                                                          cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGTAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcg                                           <  2:1399155/149‑1 (MQ=255)
                                                                                                          cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcg                                           <  2:186070/149‑1 (MQ=255)
                                                                                                           gTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcgc                                          <  1:1244034/149‑1 (MQ=255)
                                                                                                           gTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTACACTATGGTGACAGGGATTTTTTGTTTTATGGAAgcgc                                          >  2:496554/1‑149 (MQ=255)
                                                                                                                ttGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTc                                     >  1:1349350/1‑149 (MQ=255)
                                                                                                                ttGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTc                                     <  1:930448/149‑1 (MQ=255)
                                                                                                                ttGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTc                                     <  2:425709/149‑1 (MQ=255)
                                                                                                                 tGACAAATTGGCACTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTcc                                    <  1:1315877/149‑1 (MQ=255)
                                                                                                                 tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTTGACGATGGTGACAGTGATTTTTTGTTTTATGGAAGCGCGATTcc                                    <  1:678702/149‑1 (MQ=255)
                                                                                                                  gACAAATTGGCAATATGATTACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGGAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                   >  1:1428023/1‑149 (MQ=255)
                                                                                                                     aaaTTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGaa                                <  1:723205/149‑1 (MQ=255)
                                                                                                                     aaaTTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTCACGGAAGAATAAAAGTAGAAAAAAGCTAAACAAGCGAATGTAGACGATGGTGAGAGGCATTATTTTGTTTAGGGAATCGCGATTCCAGaa                                >  2:707712/1‑149 (MQ=255)
                                                                                                                       aTTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACt                              >  1:824115/1‑149 (MQ=255)
                                                                                                                        ttGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTg                             <  2:1153781/149‑1 (MQ=255)
                                                                                                                             aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAg                        >  2:1526091/1‑149 (MQ=255)
                                                                                                                               tatGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGAc                      <  1:319588/149‑1 (MQ=255)
                                                                                                                                 tGATAACATCCGTTGACAGAGTGGCAACGGATGAATGTAGCTGTGTGACGGCAGCAGAAACGTAGACAAACGCGAGACAAGCGAATGTCGACGATGGTGACAGGGCTGTTTTGGTTTATGGAAGAGCGATTCCAGAACTGGGCAGACAt                    >  1:275024/1‑149 (MQ=255)
                                                                                                                                  gATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                   >  2:175024/1‑149 (MQ=255)
                                                                                                                                   aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtta                  >  2:80757/1‑148 (MQ=255)
                                                                                                                                      tcATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCg               >  1:346832/2‑149 (MQ=255)
                                                                                                                                      aCATCCGTTGCCAGATTGGAAAGGGATGAATTTATCTGTGTGACGGACGAATAACCGTACTAAAACGCTAAACAAGAGAATGTAGACGATGGTGACTGGGATTTTTTGTTTTTTTGAAGCGCGATTCCAGAACTGGGCAGACATTGCCg               <  1:1378257/149‑1 (MQ=255)
                                                                                                                                      aCATCCCTTGCAAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCg               <  2:379633/149‑1 (MQ=255)
                                                                                                                                       cATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCgg              >  1:1444470/1‑149 (MQ=255)
                                                                                                                                        aTCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGTGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa             >  2:223127/1‑149 (MQ=255)
                                                                                                                                        aTCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGTAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa             >  2:6916/1‑149 (MQ=255)
                                                                                                                                        aTCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa             >  2:405415/1‑149 (MQ=255)
                                                                                                                                         tCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGAt            >  1:1150474/1‑149 (MQ=255)
                                                                                                                                           cGTTGCCAATCTGGCAACGGATGAATCTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGCCGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc          >  1:1200475/1‑149 (MQ=255)
                                                                                                                                             ttGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGa        >  1:1009744/1‑149 (MQ=255)
                                                                                                                                             ttGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGa        >  1:536324/1‑149 (MQ=255)
                                                                                                                                                ccAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGc     >  2:282710/1‑149 (MQ=255)
                                                                                                                                                ccAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAAGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGc     >  1:741631/1‑149 (MQ=255)
                                                                                                                                                  aGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGAAGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCtt   >  2:943234/1‑149 (MQ=255)
                                                                                                                                                   gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTg  <  1:282710/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTG  >  NC_000913/3234359‑3234654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: