Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,952 A→G 5.5% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9520AG 5.5% 277.3 / 6.7 109intergenic (‑41/+49)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base A (47/52);  new base G (3/3);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 110 total aligned reads displayed.

AGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAA  >  NC_000913/3324806‑3325100
                                                                                                                                                  |                                                                                                                                                    
aGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGc                                                                                                                                                    <  1:1149521/149‑1 (MQ=255)
aGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGc                                                                                                                                                    <  2:8702/149‑1 (MQ=255)
  ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACTTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCAc                                                                                                                                                  <  1:638340/149‑1 (MQ=255)
  ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCAc                                                                                                                                                  <  2:1271922/149‑1 (MQ=255)
         tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                           >  2:1192835/1‑149 (MQ=255)
          gcgaccaTCCGAAAAGGAATCAGGGGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                          <  2:1484124/146‑1 (MQ=255)
           ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                         >  2:325128/1‑149 (MQ=255)
            caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGATTCATGATGTTATCCCTGGTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                        >  2:600169/1‑149 (MQ=255)
               caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtttt                                                                                                                                     >  1:283897/1‑149 (MQ=255)
                aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                    >  2:90459/1‑149 (MQ=255)
                aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                    >  2:774008/1‑149 (MQ=255)
                 tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtttttt                                                                                                                                   <  1:300916/149‑3 (MQ=255)
                  cctaaaaGGATTCAGGCGTGAGGTTGTGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGAATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                  <  1:930749/146‑1 (MQ=255)
                  ccGAAAAGGAATCAGGCGGGACGTTGAGTCTCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                                  <  1:1065133/149‑4 (MQ=255)
                   cgaatcggAATCCGGCGGTACGTTGAGGATCCCCATTCCGGGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAACGAGTTTCATGATGTTATCCCTGGTATGAATAGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGt                                                                                                                                 <  1:859544/143‑1 (MQ=255)
                   cGAATAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGATTTCGGt                                                                                                                                 >  2:175948/1‑149 (MQ=255)
                   cGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                 <  1:972158/149‑1 (MQ=255)
                    gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTa                                                                                                                                >  1:131722/1‑149 (MQ=255)
                    gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGCCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCCTGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCCGAGCACGCCCCGGGCGTTTCggga                                                                                                                                >  2:1412857/1‑147 (MQ=255)
                     aaaaGGAATCAGGCGTGTCGTTCAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTAGCCTTGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTCATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                               <  2:1185597/149‑1 (MQ=255)
                     aaaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                               >  2:139890/1‑149 (MQ=255)
                      aaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttcttat                                                                                                                              <  1:409496/149‑8 (MQ=39)
                       aaGGAATCAGGCGTGACGGTGAGGATCCCCCTTACGGGACGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGTAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACaa                                                                                                                             <  2:719678/149‑1 (MQ=255)
                         ggAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAAt                                                                                                                           <  1:630448/149‑1 (MQ=255)
                          taaTCAGGCGGGACGGTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCAGGGCAAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                          <  1:797268/148‑1 (MQ=255)
                          gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                          <  1:152370/149‑1 (MQ=255)
                          gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                          <  1:184023/149‑1 (MQ=255)
                             tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                       <  1:1108982/149‑1 (MQ=255)
                             tCAGGCGTGACGTTGAGGATCCCCAATACGTTAGGATGGCTAAGGTCAAGTGAATTACCCTGGGCAAAGAGTTTCATGATGTTATCCATGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGCGTTTTCTGTACAAATAccg                                                                                                                       >  2:951675/1‑147 (MQ=255)
                                  ctggACGTTGAGGATCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                  <  2:1423929/146‑1 (MQ=255)
                                    tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                          <  2:1497399/123‑1 (MQ=255)
                                    tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                          >  1:1497399/1‑123 (MQ=255)
                                           gAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                         >  1:1435650/1‑149 (MQ=255)
                                           gAGGATCCCCAATACGTGAGGATGGCTAACGTCAAGTGAAGTACCCTGGGCTAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCACCGGGGTTTTCGGTACAAATACAGACATCTGTAGCggg                                                                                                         >  2:142281/1‑149 (MQ=255)
                                            aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGa                                                                                                        >  1:980136/1‑149 (MQ=255)
                                             ggATCGCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGTaa                                                                                                       <  1:24666/149‑1 (MQ=255)
                                              gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAAAGAGTTTCAGGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTTCAAATACAGTCATCTGATGCGGGAac                                                                                                      >  1:1477071/1‑149 (MQ=255)
                                              gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                      <  1:423165/149‑1 (MQ=255)
                                              gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                      >  2:256299/1‑149 (MQ=255)
                                                 ccccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAAATGATAAGAAAAAACGCCGGAGCACGCCCCTGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAactta                                                                                                   <  1:1285629/149‑1 (MQ=255)
                                                    cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                <  2:1330338/149‑1 (MQ=255)
                                                    cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                <  1:883977/149‑1 (MQ=255)
                                                          gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCGAGTCGcc                                                                                          >  1:1570363/1‑149 (MQ=255)
                                                             tggatggatAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGGAATCTGATGCGGGAACTTACTTGTCGCGTaa                                                                                       <  2:511881/148‑1 (MQ=255)
                                                              ggATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAAc                                                                                      <  2:997346/149‑1 (MQ=255)
                                                                    cTAAGGTCCAGTGGAGTCCCCTGGGCTAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGctct                                                                                >  2:249670/1‑149 (MQ=255)
                                                                     tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                               >  2:1470389/1‑149 (MQ=255)
                                                                     tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                               >  1:754417/1‑149 (MQ=255)
                                                                       aGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAc                                                                             >  1:922693/1‑149 (MQ=255)
                                                                          tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGTTGTTATGCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                          <  1:1314662/149‑1 (MQ=255)
                                                                          tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTACCTGCGCTGACATg                                                                          >  1:1088647/1‑149 (MQ=255)
                                                                            cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                        <  2:276091/149‑1 (MQ=255)
                                                                            cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                        <  2:64440/149‑1 (MQ=255)
                                                                            cAGTGAAGTACCCTGGGCAAACAGTATCATGATGGTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                        <  2:141457/149‑1 (MQ=255)
                                                                             aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCACGGAGCAAGCCCCGGGGTTTTCGTTACAAATAAAGACATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAAATGgtg                                                                       >  2:1517771/1‑149 (MQ=255)
                                                                              gTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                      <  1:678695/149‑1 (MQ=255)
                                                                                   gTACCCTTGGCATAGAGTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACATTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                 <  2:918392/149‑1 (MQ=255)
                                                                                   gTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                 >  1:326895/1‑149 (MQ=255)
                                                                                    tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                >  1:734380/1‑149 (MQ=255)
                                                                                    tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                >  1:1090133/1‑149 (MQ=255)
                                                                                    tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                >  1:1335701/1‑149 (MQ=255)
                                                                                    tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                <  2:3991/149‑1 (MQ=255)
                                                                                      cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                              >  2:1098952/1‑149 (MQ=255)
                                                                                      cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCCCCGGGGTTTTCGGTACACATACAGTAATCTGATGCGGGAACTTACTTGTCGCCTAACGTCTCTGCCATGGTTTTAcactgg                                                              >  2:1383619/1‑144 (MQ=255)
                                                                                        cTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCCGCTGATCCGGTAACTAACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGtt                                                            >  2:655617/1‑149 (MQ=255)
                                                                                            gCAAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAAGTCCGGATGTCGCCCGGGGGTTTTCGGTAGAAATACAGTCATCTGTTGCGGGAACTTACTTGTCGCCTTAGTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                        <  1:321994/149‑1 (MQ=255)
                                                                                              aaaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGt                                                      >  2:593477/1‑149 (MQ=255)
                                                                                                    tttCATGATGTTATCCCTGGTATTAATTGATAAGAAAAAACCCATGAGCACGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                <  1:892233/149‑1 (MQ=255)
                                                                                                     ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATCCAGTCATCTGATGCGGGAAATTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                               >  2:412901/1‑149 (MQ=255)
                                                                                                     ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                               <  1:997493/149‑1 (MQ=255)
                                                                                                     ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGAACGCCCCGGGTTTTTCGGTACAAATACAGTCATCTGAGGCGGGAACTTCCTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGt                                               <  1:858210/149‑1 (MQ=255)
                                                                                                      tCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGtt                                              <  1:499630/149‑1 (MQ=255)
                                                                                                             ttttacccTGGTATGTATTGATTCTAAACAACCCCGGCGCTCTCGCCGTGGTTTTCTGTACTAATTCAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGGGTACGCGGTTCATCAAc                                       <  2:784153/144‑1 (MQ=255)
                                                                                                             gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                       <  2:289314/149‑1 (MQ=255)
                                                                                                                 tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGa                                   <  2:946681/149‑1 (MQ=255)
                                                                                                                        tATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                            <  1:1098952/149‑1 (MQ=255)
                                                                                                                          tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCCTCCACCGGACGAGGAGcc                          >  1:760577/1‑149 (MQ=255)
                                                                                                                           gAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCt                         >  1:940460/1‑149 (MQ=255)
                                                                                                                             aTTGATTAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATATGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTCCGCGGTTCATCAACCGGACGAGGAGCCttt                       >  1:631507/1‑149 (MQ=255)
                                                                                                                              ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                      <  2:296355/149‑1 (MQ=255)
                                                                                                                              ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                      >  2:415073/1‑149 (MQ=255)
                                                                                                                              ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACACTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                      <  1:90459/149‑1 (MQ=255)
                                                                                                                               tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                     <  1:325128/149‑1 (MQ=255)
                                                                                                                                gATAAGACAACACGCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTGATCTGATGCGGGAACTTCCTTGTCGCCGAACTGCTCTGACATGGTGGTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                    <  2:362560/149‑1 (MQ=255)
                                                                                                                                 aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc                   >  2:30006/1‑149 (MQ=255)
                                                                                                                                  tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt                  >  2:22678/1‑149 (MQ=255)
                                                                                                                                   aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTa                 <  1:1192835/149‑1 (MQ=255)
                                                                                                                                    aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGGCTTTGGACTAc                >  1:411166/1‑149 (MQ=255)
                                                                                                                                    aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                <  2:1090133/149‑1 (MQ=255)
                                                                                                                                    aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                <  1:1344858/149‑1 (MQ=255)
                                                                                                                                    aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTAATCAACCGGACGAGGAGCCTTTGGACTAc                <  1:600169/149‑1 (MQ=255)
                                                                                                                                    aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGAGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                >  2:566806/1‑149 (MQ=255)
                                                                                                                                     ccgaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc               >  1:289411/4‑149 (MQ=255)
                                                                                                                                         aaaCCCCGGAGCACGCCCCGGGGTTGTCGGTACAACTACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTg           >  2:854701/1‑149 (MQ=255)
                                                                                                                                           acgctggAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGTGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc         <  2:1262953/144‑1 (MQ=255)
                                                                                                                                           aCCCCGGACCACGCCCCGGGGTTTTCGGTACAACTACACGCATCTGATGCGGGCACCTACTTGTCGCCTACCTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc         <  1:1408934/149‑1 (MQ=255)
                                                                                                                                             cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc       >  1:929590/1‑149 (MQ=255)
                                                                                                                                             cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc       >  1:1425563/1‑149 (MQ=255)
                                                                                                                                             cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc       >  1:525851/1‑149 (MQ=255)
                                                                                                                                                  aGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGaa  >  2:570237/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAA  >  NC_000913/3324806‑3325100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: