Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,955 A→G 5.7% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9550AG 5.7% 274.5 / 7.3 109intergenic (‑44/+46)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base A (47/51);  new base G (3/3);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 110 total aligned reads displayed.

TTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTG  >  NC_000913/3324808‑3325103
                                                                                                                                                   |                                                                                                                                                    
ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACTTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCAc                                                                                                                                                     <  1:638340/149‑1 (MQ=255)
ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCAc                                                                                                                                                     <  2:1271922/149‑1 (MQ=255)
       tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                              >  2:1192835/1‑149 (MQ=255)
        gcgaccaTCCGAAAAGGAATCAGGGGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                             <  2:1484124/146‑1 (MQ=255)
         ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                            >  2:325128/1‑149 (MQ=255)
          caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGATTCATGATGTTATCCCTGGTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                           >  2:600169/1‑149 (MQ=255)
            ccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGATCACGCCCCGGGGttt                                                                                                                                         >  2:155115/1‑149 (MQ=255)
             caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtttt                                                                                                                                        >  1:283897/1‑149 (MQ=255)
              aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                       >  2:90459/1‑149 (MQ=255)
              aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                       >  2:774008/1‑149 (MQ=255)
               tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtttttt                                                                                                                                      <  1:300916/149‑3 (MQ=255)
                cctaaaaGGATTCAGGCGTGAGGTTGTGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGAATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                     <  1:930749/146‑1 (MQ=255)
                ccGAAAAGGAATCAGGCGGGACGTTGAGTCTCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                                     <  1:1065133/149‑4 (MQ=255)
                 cgaatcggAATCCGGCGGTACGTTGAGGATCCCCATTCCGGGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAACGAGTTTCATGATGTTATCCCTGGTATGAATAGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGt                                                                                                                                    <  1:859544/143‑1 (MQ=255)
                 cGAATAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGATTTCGGt                                                                                                                                    >  2:175948/1‑149 (MQ=255)
                 cGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                    <  1:972158/149‑1 (MQ=255)
                  gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTa                                                                                                                                   >  1:131722/1‑149 (MQ=255)
                  gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGCCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCCTGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCCGAGCACGCCCCGGGCGTTTCggga                                                                                                                                   >  2:1412857/1‑147 (MQ=255)
                   aaaaGGAATCAGGCGTGTCGTTCAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTAGCCTTGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTCATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                  <  2:1185597/149‑1 (MQ=255)
                   aaaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                  >  2:139890/1‑149 (MQ=255)
                    aaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttcttat                                                                                                                                 <  1:409496/149‑8 (MQ=39)
                     aaGGAATCAGGCGTGACGGTGAGGATCCCCCTTACGGGACGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGTAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACaa                                                                                                                                <  2:719678/149‑1 (MQ=255)
                       ggAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAAt                                                                                                                              <  1:630448/149‑1 (MQ=255)
                        taaTCAGGCGGGACGGTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCAGGGCAAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                             <  1:797268/148‑1 (MQ=255)
                        gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                             <  1:152370/149‑1 (MQ=255)
                        gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                             <  1:184023/149‑1 (MQ=255)
                           tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                          >  2:391396/1‑149 (MQ=255)
                           tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                          <  1:1108982/149‑1 (MQ=255)
                           tCAGGCGTGACGTTGAGGATCCCCAATACGTTAGGATGGCTAAGGTCAAGTGAATTACCCTGGGCAAAGAGTTTCATGATGTTATCCATGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGCGTTTTCTGTACAAATAccg                                                                                                                          >  2:951675/1‑147 (MQ=255)
                                ctggACGTTGAGGATCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                     <  2:1423929/146‑1 (MQ=255)
                                  tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                             <  2:1497399/123‑1 (MQ=255)
                                  tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                             >  1:1497399/1‑123 (MQ=255)
                                    aCGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAt                                                                                                                 >  1:13291/1‑149 (MQ=255)
                                         gAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                            >  1:1435650/1‑149 (MQ=255)
                                         gAGGATCCCCAATACGTGAGGATGGCTAACGTCAAGTGAAGTACCCTGGGCTAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCACCGGGGTTTTCGGTACAAATACAGACATCTGTAGCggg                                                                                                            >  2:142281/1‑149 (MQ=255)
                                          aGGATCCCCATTACGTGCGGATGGCTAAGGTCCATTGAAGTACCCTGGGCAAAGAGTTTCTTGATGGTTTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAATTACAGTGATCTGATGCGGGa                                                                                                           <  2:675728/149‑1 (MQ=255)
                                          aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGa                                                                                                           >  1:980136/1‑149 (MQ=255)
                                           ggATCGCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGTaa                                                                                                          <  1:24666/149‑1 (MQ=255)
                                            taTCCCCATTACGTGAGGATTGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCTGTACAAATACAGTCATCTGATGCGGGAac                                                                                                         <  1:962167/148‑1 (MQ=255)
                                            gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAAAGAGTTTCAGGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTTCAAATACAGTCATCTGATGCGGGAac                                                                                                         >  1:1477071/1‑149 (MQ=255)
                                            gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                         <  1:423165/149‑1 (MQ=255)
                                            gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                         >  2:1344858/1‑149 (MQ=255)
                                            gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                         >  2:256299/1‑149 (MQ=255)
                                               ccccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAAATGATAAGAAAAAACGCCGGAGCACGCCCCTGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAactta                                                                                                      <  1:1285629/149‑1 (MQ=255)
                                                  cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                   <  1:883977/149‑1 (MQ=255)
                                                        gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCGAGTCGcc                                                                                             >  1:1570363/1‑149 (MQ=255)
                                                           tggatggatAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGGAATCTGATGCGGGAACTTACTTGTCGCGTaa                                                                                          <  2:511881/148‑1 (MQ=255)
                                                            ggATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAAc                                                                                         <  2:997346/149‑1 (MQ=255)
                                                                  cTAAGGTCCAGTGGAGTCCCCTGGGCTAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGctct                                                                                   >  2:249670/1‑149 (MQ=255)
                                                                   tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                  >  2:1470389/1‑149 (MQ=255)
                                                                   tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                  >  1:754417/1‑149 (MQ=255)
                                                                     aGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAc                                                                                >  1:922693/1‑149 (MQ=255)
                                                                        tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGTTGTTATGCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                             <  1:1314662/149‑1 (MQ=255)
                                                                        tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTACCTGCGCTGACATg                                                                             >  1:1088647/1‑149 (MQ=255)
                                                                          cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                           <  2:276091/149‑1 (MQ=255)
                                                                          cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                           <  2:64440/149‑1 (MQ=255)
                                                                          cAGTGAAGTACCCTGGGCAAACAGTATCATGATGGTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                           <  2:141457/149‑1 (MQ=255)
                                                                            gTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                         <  1:678695/149‑1 (MQ=255)
                                                                                 gTACCCTTGGCATAGAGTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACATTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                    <  2:918392/149‑1 (MQ=255)
                                                                                 gTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                    >  1:326895/1‑149 (MQ=255)
                                                                                  tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                   <  2:3991/149‑1 (MQ=255)
                                                                                  tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                   >  1:1090133/1‑149 (MQ=255)
                                                                                  tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                   >  1:734380/1‑149 (MQ=255)
                                                                                    cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                 >  2:1098952/1‑149 (MQ=255)
                                                                                    cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCCCCGGGGTTTTCGGTACACATACAGTAATCTGATGCGGGAACTTACTTGTCGCCTAACGTCTCTGCCATGGTTTTAcactgg                                                                 >  2:1383619/1‑144 (MQ=255)
                                                                                          gCAAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAAGTCCGGATGTCGCCCGGGGGTTTTCGGTAGAAATACAGTCATCTGTTGCGGGAACTTACTTGTCGCCTTAGTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                           <  1:321994/149‑1 (MQ=255)
                                                                                            aaaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGt                                                         >  2:593477/1‑149 (MQ=255)
                                                                                                  tttCATGATGTTATCCCTGGTATTAATTGATAAGAAAAAACCCATGAGCACGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                   <  1:892233/149‑1 (MQ=255)
                                                                                                   ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATCCAGTCATCTGATGCGGGAAATTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                  >  2:412901/1‑149 (MQ=255)
                                                                                                   ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                  <  1:997493/149‑1 (MQ=255)
                                                                                                   ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGAACGCCCCGGGTTTTTCGGTACAAATACAGTCATCTGAGGCGGGAACTTCCTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGt                                                  <  1:858210/149‑1 (MQ=255)
                                                                                                    tCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGtt                                                 <  1:499630/149‑1 (MQ=255)
                                                                                                           ttttacccTGGTATGTATTGATTCTAAACAACCCCGGCGCTCTCGCCGTGGTTTTCTGTACTAATTCAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGGGTACGCGGTTCATCAAc                                          <  2:784153/144‑1 (MQ=255)
                                                                                                           gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                          <  2:289314/149‑1 (MQ=255)
                                                                                                               tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGa                                      <  2:946681/149‑1 (MQ=255)
                                                                                                                      tATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                               <  1:1098952/149‑1 (MQ=255)
                                                                                                                           aTTGATTAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATATGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTCCGCGGTTCATCAACCGGACGAGGAGCCttt                          >  1:631507/1‑149 (MQ=255)
                                                                                                                            ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                         >  2:415073/1‑149 (MQ=255)
                                                                                                                            ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                         <  2:296355/149‑1 (MQ=255)
                                                                                                                            ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACACTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                         <  1:90459/149‑1 (MQ=255)
                                                                                                                             tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                        <  1:325128/149‑1 (MQ=255)
                                                                                                                               aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc                      >  2:30006/1‑149 (MQ=255)
                                                                                                                                tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt                     >  2:22678/1‑149 (MQ=255)
                                                                                                                                 aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTa                    <  1:1192835/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGGCTTTGGACTAc                   >  1:411166/1‑149 (MQ=255)
                                                                                                                                  aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                   <  2:1090133/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                   <  1:1344858/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTAATCAACCGGACGAGGAGCCTTTGGACTAc                   <  1:600169/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGAGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                   >  2:566806/1‑149 (MQ=255)
                                                                                                                                   ccgaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                  >  1:289411/4‑149 (MQ=255)
                                                                                                                                       aaaCCCCGGAGCACGCCCCGGGGTTGTCGGTACAACTACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTg              >  2:854701/1‑149 (MQ=255)
                                                                                                                                         acgctggAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGTGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc            <  2:1262953/144‑1 (MQ=255)
                                                                                                                                         aCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc            <  1:774008/149‑1 (MQ=255)
                                                                                                                                         aCCCCGGACCACGCCCCGGGGTTTTCGGTACAACTACACGCATCTGATGCGGGCACCTACTTGTCGCCTACCTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc            <  1:1408934/149‑1 (MQ=255)
                                                                                                                                           cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc          >  1:929590/1‑149 (MQ=255)
                                                                                                                                           cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc          >  1:1425563/1‑149 (MQ=255)
                                                                                                                                           cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc          >  1:525851/1‑149 (MQ=255)
                                                                                                                                                aGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGaa     >  2:570237/1‑149 (MQ=255)
                                                                                                                                                  cACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAAtt   <  1:136969/149‑1 (MQ=255)
                                                                                                                                                   aCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTg  <  1:757378/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTG  >  NC_000913/3324808‑3325103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: