Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,959 C→T 5.5% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9590CT 5.5% 270.8 / 4.3 110intergenic (‑48/+42)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base C (48/54);  new base T (3/3);  total (52/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 110 total aligned reads displayed.

TGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324815‑3325105
                                                                                                                                                |                                                                                                                                                  
tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                                >  2:1192835/1‑149 (MQ=255)
 gcgaccaTCCGAAAAGGAATCAGGGGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               <  2:1484124/146‑1 (MQ=255)
  ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                              >  2:325128/1‑149 (MQ=255)
   caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGATTCATGATGTTATCCCTGGTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                             >  2:600169/1‑149 (MQ=255)
      caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtttt                                                                                                                                          >  1:283897/1‑149 (MQ=255)
       aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                         >  2:90459/1‑149 (MQ=255)
       aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                         >  2:774008/1‑149 (MQ=255)
        tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtttttt                                                                                                                                        <  1:300916/149‑3 (MQ=255)
         cctaaaaGGATTCAGGCGTGAGGTTGTGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGAATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                       <  1:930749/146‑1 (MQ=255)
         ccGAAAAGGAATCAGGCGGGACGTTGAGTCTCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                                       <  1:1065133/149‑4 (MQ=255)
          cgaatcggAATCCGGCGGTACGTTGAGGATCCCCATTCCGGGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAACGAGTTTCATGATGTTATCCCTGGTATGAATAGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGt                                                                                                                                      <  1:859544/143‑1 (MQ=255)
          cGAATAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGATTTCGGt                                                                                                                                      >  2:175948/1‑149 (MQ=255)
          cGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                      <  1:972158/149‑1 (MQ=255)
           gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTa                                                                                                                                     >  1:131722/1‑149 (MQ=255)
           gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGCCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCCTGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCCGAGCACGCCCCGGGCGTTTCggga                                                                                                                                     >  2:1412857/1‑147 (MQ=255)
            aaaaGGAATCAGGCGTGTCGTTCAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTAGCCTTGGCAAAGAGTTTCATGATGTTATCCCTGGTATGATTTCATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                    <  2:1185597/149‑1 (MQ=255)
            aaaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                    >  2:139890/1‑149 (MQ=255)
             aaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttcttat                                                                                                                                   <  1:409496/149‑8 (MQ=39)
              aaGGAATCAGGCGTGACGGTGAGGATCCCCCTTACGGGACGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGTAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACaa                                                                                                                                  <  2:719678/149‑1 (MQ=255)
                ggAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAAt                                                                                                                                <  1:630448/149‑1 (MQ=255)
                 taaTCAGGCGGGACGGTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCAGGGCAAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                               <  1:797268/148‑1 (MQ=255)
                 gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                               <  1:152370/149‑1 (MQ=255)
                 gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                               <  1:184023/149‑1 (MQ=255)
                    tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                            >  2:391396/1‑149 (MQ=255)
                    tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                            <  1:1108982/149‑1 (MQ=255)
                    tCAGGCGTGACGTTGAGGATCCCCAATACGTTAGGATGGCTAAGGTCAAGTGAATTACCCTGGGCAAAGAGTTTCATGATGTTATCCATGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGCGTTTTCTGTACAAATAccg                                                                                                                            >  2:951675/1‑147 (MQ=255)
                         ctggACGTTGAGGATCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                       <  2:1423929/146‑1 (MQ=255)
                           tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               >  1:1497399/1‑123 (MQ=255)
                           tGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               <  2:1497399/123‑1 (MQ=255)
                             aCGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAt                                                                                                                   >  1:13291/1‑149 (MQ=255)
                                  gAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                              >  1:1435650/1‑149 (MQ=255)
                                  gAGGATCCCCAATACGTGAGGATGGCTAACGTCAAGTGAAGTACCCTGGGCTAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCACCGGGGTTTTCGGTACAAATACAGACATCTGTAGCggg                                                                                                              >  2:142281/1‑149 (MQ=255)
                                   aGGATCCCCATTACGTGCGGATGGCTAAGGTCCATTGAAGTACCCTGGGCAAAGAGTTTCTTGATGGTTTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAATTACAGTGATCTGATGCGGGa                                                                                                             <  2:675728/149‑1 (MQ=255)
                                   aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGa                                                                                                             >  1:980136/1‑149 (MQ=255)
                                    ggATCGCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGTaa                                                                                                            <  1:24666/149‑1 (MQ=255)
                                     taTCCCCATTACGTGAGGATTGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCTGTACAAATACAGTCATCTGATGCGGGAac                                                                                                           <  1:962167/148‑1 (MQ=255)
                                     gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTCCCCTGGGCAAAGAGTTTCAGGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTTCAAATACAGTCATCTGATGCGGGAac                                                                                                           >  1:1477071/1‑149 (MQ=255)
                                     gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                           <  1:423165/149‑1 (MQ=255)
                                     gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                           >  2:256299/1‑149 (MQ=255)
                                        ccccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAAATGATAAGAAAAAACGCCGGAGCACGCCCCTGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAactta                                                                                                        <  1:1285629/149‑1 (MQ=255)
                                           cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                     <  2:1330338/149‑1 (MQ=255)
                                           cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                     <  1:883977/149‑1 (MQ=255)
                                                 gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCGAGTCGcc                                                                                               >  1:1570363/1‑149 (MQ=255)
                                                    tggatggatAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCGTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGGAATCTGATGCGGGAACTTACTTGTCGCGTaa                                                                                            <  2:511881/148‑1 (MQ=255)
                                                     ggATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAAc                                                                                           <  2:997346/149‑1 (MQ=255)
                                                           cTAAGGTCCAGTGGAGTCCCCTGGGCTAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGctct                                                                                     >  2:249670/1‑149 (MQ=255)
                                                            tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                    >  2:1470389/1‑149 (MQ=255)
                                                            tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                    >  1:754417/1‑149 (MQ=255)
                                                              aGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAc                                                                                  >  1:922693/1‑149 (MQ=255)
                                                                 tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGTTGTTATGCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                               <  1:1314662/149‑1 (MQ=255)
                                                                 tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTACCTGCGCTGACATg                                                                               >  1:1088647/1‑149 (MQ=255)
                                                                   cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                             <  2:276091/149‑1 (MQ=255)
                                                                   cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                             <  2:64440/149‑1 (MQ=255)
                                                                   cAGTGAAGTACCCTGGGCAAACAGTATCATGATGGTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                             <  2:141457/149‑1 (MQ=255)
                                                                    aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCACGGAGCAAGCCCCGGGGTTTTCGTTACAAATAAAGACATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAAATGgtg                                                                            >  2:1517771/1‑149 (MQ=255)
                                                                     gTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           <  1:678695/149‑1 (MQ=255)
                                                                          gTACCCTTGGCATAGAGTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACATTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                      <  2:918392/149‑1 (MQ=255)
                                                                          gTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                                      >  1:326895/1‑149 (MQ=255)
                                                                           tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                     >  1:1090133/1‑149 (MQ=255)
                                                                           tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                     >  1:734380/1‑149 (MQ=255)
                                                                           tACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCg                                                                     <  2:3991/149‑1 (MQ=255)
                                                                             cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   >  2:1098952/1‑149 (MQ=255)
                                                                             cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCAGGAGCACGCCCCGGGGTTTTCGGTACACATACAGTAATCTGATGCGGGAACTTACTTGTCGCCTAACGTCTCTGCCATGGTTTTAcactgg                                                                   >  2:1383619/1‑144 (MQ=255)
                                                                                   gCAAAGAGTTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAAGTCCGGATGTCGCCCGGGGGTTTTCGGTAGAAATACAGTCATCTGTTGCGGGAACTTACTTGTCGCCTTAGTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                             <  1:321994/149‑1 (MQ=255)
                                                                                     aaaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGt                                                           >  2:593477/1‑149 (MQ=255)
                                                                                           tttCATGATGTTATCCCTGGTATTAATTGATAAGAAAAAACCCATGAGCACGCCCCGGGGTTTTCGGTACAAATAGAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                     <  1:892233/149‑1 (MQ=255)
                                                                                            ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATCCAGTCATCTGATGCGGGAAATTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                    >  2:412901/1‑149 (MQ=255)
                                                                                            ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                    <  1:997493/149‑1 (MQ=255)
                                                                                            ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGAACGCCCCGGGTTTTTCGGTACAAATACAGTCATCTGAGGCGGGAACTTCCTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGt                                                    <  1:858210/149‑1 (MQ=255)
                                                                                             tCATGATGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGCGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGtt                                                   <  1:499630/149‑1 (MQ=255)
                                                                                                    ttttacccTGGTATGTATTGATTCTAAACAACCCCGGCGCTCTCGCCGTGGTTTTCTGTACTAATTCAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGGGTACGCGGTTCATCAAc                                            <  2:784153/144‑1 (MQ=255)
                                                                                                    gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                            <  2:289314/149‑1 (MQ=255)
                                                                                                        tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGa                                        <  2:946681/149‑1 (MQ=255)
                                                                                                               tATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                                 <  1:1098952/149‑1 (MQ=255)
                                                                                                                 tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCCTCCACCGGACGAGGAGcc                               >  1:760577/1‑149 (MQ=255)
                                                                                                                    aTTGATTAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATATGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTCCGCGGTTCATCAACCGGACGAGGAGCCttt                            >  1:631507/1‑149 (MQ=255)
                                                                                                                     ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                           <  2:296355/149‑1 (MQ=255)
                                                                                                                     ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                           >  2:415073/1‑149 (MQ=255)
                                                                                                                     ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACACTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                           <  1:90459/149‑1 (MQ=255)
                                                                                                                      tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCGCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                          <  1:325128/149‑1 (MQ=255)
                                                                                                                        aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc                        >  2:30006/1‑149 (MQ=255)
                                                                                                                         tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt                       >  2:22678/1‑149 (MQ=255)
                                                                                                                          aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTa                      <  1:1192835/149‑1 (MQ=255)
                                                                                                                           aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGGCTTTGGACTAc                     >  1:411166/1‑149 (MQ=255)
                                                                                                                           aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                     <  2:1090133/149‑1 (MQ=255)
                                                                                                                           aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                     <  1:1344858/149‑1 (MQ=255)
                                                                                                                           aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTAATCAACCGGACGAGGAGCCTTTGGACTAc                     <  1:600169/149‑1 (MQ=255)
                                                                                                                           aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGAGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc                     >  2:566806/1‑149 (MQ=255)
                                                                                                                            ccgaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                    >  1:289411/4‑149 (MQ=255)
                                                                                                                                aaaCCCCGGAGCACGCCCCGGGGTTGTCGGTACAACTACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTg                >  2:854701/1‑149 (MQ=255)
                                                                                                                                  acgctggAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGTGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc              <  2:1262953/144‑1 (MQ=255)
                                                                                                                                  aCCCCGGACCACGCCCCGGGGTTTTCGGTACAACTACACGCATCTGATGCGGGCACCTACTTGTCGCCTACCTGCGCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc              <  1:1408934/149‑1 (MQ=255)
                                                                                                                                    cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc            >  1:929590/1‑149 (MQ=255)
                                                                                                                                    cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc            >  1:1425563/1‑149 (MQ=255)
                                                                                                                                    cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc            >  1:525851/1‑149 (MQ=255)
                                                                                                                                         aGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTAGTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGaa       >  2:570237/1‑149 (MQ=255)
                                                                                                                                           cACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAAtt     <  1:136969/149‑1 (MQ=255)
                                                                                                                                            aCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTg    <  1:757378/149‑1 (MQ=255)
                                                                                                                                             cGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGt   <  2:283897/149‑1 (MQ=255)
                                                                                                                                              gCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCAGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGtt  <  1:8690/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324815‑3325105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: