Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,982 G→C 7.5% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,9820GC 7.5% 276.1 / 13.8 104intergenic (+28/+32)yhcG/yhcHDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
Reads supporting (aligned to +/- strand):  ref base G (60/36);  new base C (4/4);  total (64/40)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 109 total aligned reads displayed.

GTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368836‑3369130
                                                                                                                                                  |                                                                                                                                                    
gTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                    >  1:1511632/1‑149 (MQ=255)
 tttGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtgt                                                                                                                                                   >  1:1095555/1‑149 (MQ=255)
 tttGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtgt                                                                                                                                                   <  1:825467/149‑1 (MQ=255)
 tttGCCTAACACCGTTCAGGCAAGCTAATATAAGATGCAAAAACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtgt                                                                                                                                                   <  2:1171804/149‑1 (MQ=255)
   tGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCt                                                                                                                                                 <  1:1103154/149‑1 (MQ=255)
     ccTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGc                                                                                                                                               >  1:539723/1‑149 (MQ=255)
     ccTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGc                                                                                                                                               <  2:1056248/149‑1 (MQ=255)
      cTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGTGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCa                                                                                                                                              <  1:253484/149‑1 (MQ=255)
      cTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTACTTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              >  2:1232578/1‑149 (MQ=255)
      cTAACACCGTTCTGGCAAGCGAAGATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCGAGAGGAAGGCTATAGACGCGGTTTTTTTCAGCGGTGTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              >  2:506094/1‑149 (MQ=255)
       tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGCTGACAAACGACTCGCAGATGCACTCGCTCGAACACCGGCGGGGCGAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                             <  2:309508/149‑1 (MQ=255)
        aaCACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGa                                                                                                                                            >  1:69443/1‑149 (MQ=255)
         acacCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTTCAGACAGTGTCTGCAGaa                                                                                                                                           >  2:780753/1‑149 (MQ=255)
          cacCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGAAAGTCTCTGCAGAAt                                                                                                                                          >  2:1118901/1‑149 (MQ=255)
          cacCGGTCTGGCAAGCGAATATAAGGTGCAACTACCTCATGAGAAACGACTCGCAGATGAACTCGGTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAAt                                                                                                                                          <  2:711814/149‑1 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGTTGCAACTACCTTATGAGAAACGACTCGCAGATGAACTCGTTCTAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTGTCGAATTCTGCAGACACTGTCTGCAGAAtt                                                                                                                                         <  1:446862/149‑2 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAAtt                                                                                                                                         <  1:60355/149‑2 (MQ=255)
            cctttCTGGCATGAGAATCTAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAAttc                                                                                                                                        <  1:857162/146‑3 (MQ=255)
            ccGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGCAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCt                                                                                                                                        >  1:1084902/1‑149 (MQ=255)
             cGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGAAGACAGTGTCTGCAGAATCtt                                                                                                                                       >  1:690751/1‑149 (MQ=255)
                tCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaa                                                                                                                                    <  2:1337440/149‑1 (MQ=255)
                tCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaa                                                                                                                                    <  2:135667/149‑1 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGCACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  >  2:1494815/1‑149 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTGAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  >  1:459109/1‑149 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  <  1:682013/149‑1 (MQ=255)
                          gAATATAAGTTGCAACAACCTGCTGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTaa                                                                                                                          <  2:1419697/149‑1 (MQ=255)
                             tataAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAATGAACTAAGtt                                                                                                                       >  2:888913/1‑149 (MQ=255)
                               taAGATGCACCTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACATGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGtttt                                                                                                                     >  1:319235/1‑149 (MQ=255)
                               aaaGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTGCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGtttt                                                                                                                     <  1:1106801/148‑1 (MQ=255)
                                   aTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGCAAGGCTATAGACGCCGTTAATTTCAGCGCTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGc                                                                                                                 >  1:379520/1‑149 (MQ=255)
                                       aaCTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGCCTATAGACGCCGTTAATTTCAGCGCTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCAtt                                                                                                             >  2:353445/1‑149 (MQ=255)
                                             cTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAACTAATAAGCCATTAAATCa                                                                                                       >  2:454733/1‑149 (MQ=255)
                                              tgatgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAg                                                                                                      >  2:1296315/1‑149 (MQ=255)
                                               gatgaGAAACGACTCGCAGATGAACGCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGc                                                                                                     <  1:1569905/149‑1 (MQ=255)
                                                atgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGcc                                                                                                    <  2:437032/149‑1 (MQ=255)
                                                  gagaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCtt                                                                                                  >  1:1439615/1‑149 (MQ=255)
                                                    gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                                <  2:266392/149‑1 (MQ=255)
                                                     aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                                                               >  1:1499813/1‑149 (MQ=255)
                                                       aCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCTTTAAATCAGCCTTAACCt                                                                                             >  1:111580/1‑149 (MQ=255)
                                                         gACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTc                                                                                           >  2:798998/1‑149 (MQ=255)
                                                         gACTCGCAGATGAACTCGTTCGAACACAGGCGGGGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGGCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTc                                                                                           <  2:698517/149‑1 (MQ=255)
                                                           cTCGGAGATGAACGCGTTAGAACACTGGAGGTGCTAGAGGAAGGCTTTAGACGGCGTTAATTTCAGCGGTTTCGAATTCTGCATACAGTGTCTGCAGAATCTTTAATACGATCTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcac                                                                                         <  2:1462138/149‑1 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  2:364282/1‑149 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  2:1536036/1‑149 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  1:888760/1‑149 (MQ=255)
                                                              gCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcaccac                                                                                      >  2:1300591/1‑149 (MQ=255)
                                                                     aaCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCttt                                                                               <  1:1384969/149‑1 (MQ=255)
                                                                      aCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACTCCGTTAATTTCAGCGGGATCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCtttt                                                                              <  2:416237/149‑1 (MQ=255)
                                                                      aCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGGCGTTAATTTCTTCTGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGATCTAAGTTTTAAGACCTTAAATCTGCCTTAACGTTCACCCCAACCgttt                                                                              >  2:1378489/1‑149 (MQ=255)
                                                                          gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                          <  2:69443/149‑1 (MQ=255)
                                                                          gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                          <  1:669338/149‑1 (MQ=255)
                                                                           ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAta                                                                         >  1:25122/1‑148 (MQ=255)
                                                                               aaCACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGCCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGACATTAAATCAGCCTTAACATTCACCACACCCTTTTTAAtctctc                                                                     >  1:1398339/1‑149 (MQ=255)
                                                                               aaCACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtctctc                                                                     <  2:878987/149‑1 (MQ=255)
                                                                                acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGCCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                    >  1:894797/1‑149 (MQ=255)
                                                                                acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACCACCTTTTTAATCTCTcc                                                                    >  2:1231431/1‑149 (MQ=255)
                                                                                acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                    <  2:501667/149‑1 (MQ=255)
                                                                                acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                    <  2:769037/149‑1 (MQ=255)
                                                                                 aacaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCa                                                                   <  2:638519/148‑1 (MQ=255)
                                                                                  acaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAg                                                                  >  2:596977/1‑149 (MQ=255)
                                                                                      gCGGTGCTAGAGGAAGGCTATAGCCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCAGTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTc                                                              >  2:831056/1‑149 (MQ=255)
                                                                                        ggTGCTAGAGGAAGGCTATAGACGCCGTTAATTTAAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTATAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTAAACACAACCTTTTTAATCTCTCCAGGCTCGc                                                            >  2:275048/1‑149 (MQ=255)
                                                                                              agagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACaa                                                      <  1:1367300/149‑1 (MQ=255)
                                                                                                agGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAATGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACg                                                    <  1:888913/149‑1 (MQ=255)
                                                                                                     ggCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTATTTAATCTCACCAGGCTAGCCGACAACGCATcc                                               >  1:663411/1‑149 (MQ=255)
                                                                                                       cTATAGTCGCCGTTAATTTGACCGGGTTCGAATTCTTCAGTCAGTGTCTGCAGAATTTTTAAAACGAACTAATTTTTAATGCATTAAATCAGCCTTAAACTTCACCACAACCTTTTTAATCTCTCCATGCTCGCCGACAACGCATCCTg                                             <  2:1213758/149‑1 (MQ=255)
                                                                                                       cTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTg                                             <  1:1373564/149‑1 (MQ=255)
                                                                                                        tataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCAGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTgg                                            >  2:1508887/1‑149 (MQ=255)
                                                                                                         ataGACGCCGTTAATTTCAGCGGATTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGt                                           <  1:659386/149‑1 (MQ=255)
                                                                                                            gACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGACCTAAGTTATAAGCCATTAAATCAGCCTTAACCTTCACCCCAACCTTTTTAATCTCTCCCGGCTCGCCGACAACGCATCCTGGTTTa                                        >  2:793035/1‑149 (MQ=255)
                                                                                                            gACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACCACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTa                                        >  1:1430879/1‑149 (MQ=255)
                                                                                                             aCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAATGCATCCTGGTTTAt                                       >  2:208007/1‑149 (MQ=255)
                                                                                                             aCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTAt                                       >  1:1151421/1‑149 (MQ=255)
                                                                                                               gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     >  1:1042113/1‑149 (MQ=255)
                                                                                                               gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATAAGCCTTAACCTTCAACACAACCTTTTTAATCTCTCCAGGCTAGCCGACAACGCATCCTGGTTAATGc                                     >  1:549607/1‑149 (MQ=255)
                                                                                                               gCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     <  1:1296315/149‑1 (MQ=255)
                                                                                                                ccGTTAATTTCAGCGGTTTCGAATTCTGCAGGCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACACCCTTTTTAATCTCTCCAGGCTCGCCGAAACCGCATCCTGGTTTATTCg                                    >  2:1180663/1‑149 (MQ=255)
                                                                                                                ccGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCg                                    >  2:619832/1‑149 (MQ=255)
                                                                                                                 cGTTTATTTAAGCGGTTTAGAATTCTGAAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCAATTAAATGAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCGAGGCTCGCCGACAACGCATCCTGGTATATGCgg                                   <  2:1018610/149‑1 (MQ=255)
                                                                                                                 cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCgg                                   <  1:601135/149‑1 (MQ=255)
                                                                                                                 cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCAAGGCTCGCCGACAAAGCATCCTGGTTTATGCgg                                   <  2:1402820/149‑1 (MQ=255)
                                                                                                                   ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGATTCTTAAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTACAGCATCGCCGTCAACGCGTCCTTGTTTATGCGGtt                                 >  1:202617/1‑149 (MQ=255)
                                                                                                                   ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGtt                                 >  1:467635/1‑149 (MQ=255)
                                                                                                                   ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGtt                                 >  1:1312699/1‑149 (MQ=255)
                                                                                                                     aaTTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCa                               >  2:124340/1‑149 (MQ=255)
                                                                                                                      aTTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCAc                              >  2:50948/1‑149 (MQ=255)
                                                                                                                         tCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCTCCGACAACGCATCCTGGTATATGCGGTTCACCTg                           >  2:1126354/1‑149 (MQ=255)
                                                                                                                             cGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCAt                       <  1:1300591/149‑1 (MQ=255)
                                                                                                                                 ttCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGCACGAAGTTTTAAGCCATTAAATCAGCCTTACCCTTCTCCCCAACCTTTTTAATATCTCCCGGGTAGCCGACCACCCATCCTGGGTTATCCGGGTCACCGGCCATAAac                   >  2:172263/1‑149 (MQ=255)
                                                                                                                                 ttCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAAACTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCCCCTGGCATAAac                   >  2:172262/1‑149 (MQ=255)
                                                                                                                                  tCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAacc                  >  1:1281443/1‑148 (MQ=255)
                                                                                                                                  tCGAATTCTGCAGACAGTGTCTGCAGAATATTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAacc                  >  2:1136555/1‑148 (MQ=255)
                                                                                                                                    gAATTCTGCAGGCAGTGTCTGCAGAATCTTTAAAACGAACGAAGTTTTAAGCCATTAAATCAGCCTTACCCTTCACCACAACCATTTTAATCTCTCCAGCATGGCCGCCAACGCATCCTGGTATATGCGGTACACGGGGCATaccaacg                >  1:1054989/1‑143 (MQ=255)
                                                                                                                                     gaTTCTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACgg               >  1:1206355/2‑149 (MQ=255)
                                                                                                                                     gaTTCTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACgg               >  1:451809/2‑149 (MQ=255)
                                                                                                                                        tCTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGa            >  2:82978/1‑149 (MQ=255)
                                                                                                                                             aGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAtt       >  2:574145/1‑149 (MQ=255)
                                                                                                                                               aCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAAACAGCCTTAACCTTCACCACAACCTTTTTAATCTCACCAGGCTCGCCGACAACCCATCCTGGTATATGCGGTTCACCTGGCATAAACACGGCGAACaaacc     >  2:1207642/1‑145 (MQ=255)
                                                                                                                                                cAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTccc    <  1:596977/149‑1 (MQ=255)
                                                                                                                                                  gtgtCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCCGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGCCAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGATCATGCCCgg  >  1:1407626/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368836‑3369130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: