Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,512 A→G 5.2% intergenic (+63/+71) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,5120AG 5.2% 299.4 / 6.9 117intergenic (+63/+71)aaeR/tldDtranscriptional regulator for aaeXAB operon/putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (58/50);  new base G (2/4);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-02

Only 100 of 118 total aligned reads displayed.

GTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTC  >  NC_000913/3390366‑3390660
                                                                                                                                                  |                                                                                                                                                     
gTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCAAATTGTACTGTTACGTTGTAAAAACCTGTGCCAACGGGTTCCCCTCCCCCTAACCCTCAcc                                                                                                                                                     >  2:1518970/1‑149 (MQ=255)
    tGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCCCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCaa                                                                                                                                                 >  1:821679/1‑149 (MQ=255)
    tGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaa                                                                                                                                                 >  2:1369270/1‑149 (MQ=255)
    tGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCACGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaa                                                                                                                                                 <  1:1100940/149‑1 (MQ=255)
      aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                               <  2:242860/149‑1 (MQ=255)
         aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                            >  1:1481118/1‑149 (MQ=255)
          aCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGc                                                                                                                                           >  1:44411/1‑149 (MQ=255)
             cGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                        <  1:106533/149‑1 (MQ=255)
             cGCTGACGGATTATTTTGTTGAGGGCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                        <  1:529848/149‑1 (MQ=255)
              gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                       <  2:1221733/149‑1 (MQ=255)
              gCTGACGGATTATTTTGTTGAGCTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                       <  1:258076/149‑1 (MQ=255)
               cTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAggg                                                                                                                                      >  1:995100/1‑149 (MQ=255)
                ttactgATTATTTTGTTGAGGTCTGTAAATTGTTTCAGGAGAGGCACGGGCGCGGGAAAGAGATGTAATTCATATTGTACTGTTACGTTGTACATACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgggg                                                                                                                                     <  1:1485985/144‑1 (MQ=39)
                tGACGGAGTATTTTGTTGAGGACGGCAAATTGTTTCAGGAGATGCCCGGGCGCGGGAAAGAGAAGTAATGCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggt                                                                                                                                     <  1:391190/149‑2 (MQ=255)
                   cGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGAcc                                                                                                                                  >  2:896673/1‑149 (MQ=255)
                   cGGATCATTTTGTTGAGGTCGGTAAATTGTATCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                  <  2:622457/149‑5 (MQ=255)
                    ggATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCg                                                                                                                                 >  2:650301/1‑149 (MQ=255)
                          ttttGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTg                                                                                                                           >  2:1176177/1‑149 (MQ=255)
                              gttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACg                                                                                                                       >  1:341473/1‑149 (MQ=255)
                                  aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGa                                                                                                                   >  2:37483/1‑149 (MQ=255)
                                  aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACAGTTCGTGCACGTAGa                                                                                                                   >  1:94016/1‑149 (MQ=255)
                                        gTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGatt                                                                                                             <  2:1545618/149‑1 (MQ=255)
                                         tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGGAGAAAGatta                                                                                                            >  2:315229/1‑149 (MQ=255)
                                         tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGagta                                                                                                            >  1:1452346/1‑149 (MQ=255)
                                          aaaTTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGattaa                                                                                                           >  1:175838/1‑149 (MQ=255)
                                             ttGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCCACGGGTTCCCCTCACCCTAACCCTCACGCCAACGGGGCGAGGGGACCGTTCGTGCCCGTAGAAAGattaatta                                                                                                        >  1:887320/1‑149 (MQ=255)
                                              tctatccggAGCTGCACGCGCGCGGGAACGCGAATGACTTCATATTGTACTGTTACGTTGTACAACCCTGTGCCAAGGGGGTCCCCGCACCCTAACCCACACCCCTAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAt                                                                                                       <  2:1446595/142‑1 (MQ=255)
                                               gtctCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAAATCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                      <  1:1233361/146‑1 (MQ=255)
                                               gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                      <  1:650301/149‑1 (MQ=255)
                                                 ttCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCt                                                                                                    >  1:1233044/1‑149 (MQ=255)
                                                  tCAGGACATGCACGGGCGCGGGAAAGACAAGTAATTCATATTGTACGGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCACAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCtt                                                                                                   <  1:1066578/149‑1 (MQ=255)
                                                   cAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTc                                                                                                  <  2:669712/149‑1 (MQ=255)
                                                    aGGAGATTCACGGGCGCGGGATAGAGAAGTAATTCATATTGTACTGTTACGTTGTAGAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACGCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCt                                                                                                 <  1:289353/149‑1 (MQ=255)
                                                        gaTGCACGGGCGCGGGAACGAGAAGTAATTCATATTGTACTGTTTCGTTGTACAAACCTGTGCCAACGGGTGCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCGTTCGTGCACGTAGAAGGATAATAATCTTTCtacgtg                                                                                            <  2:1112739/149‑6 (MQ=255)
                                                           gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                          >  1:1306334/1‑149 (MQ=255)
                                                           gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                          <  1:1033424/149‑1 (MQ=255)
                                                            cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                         >  1:350083/1‑149 (MQ=255)
                                                            cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCATCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                         >  2:1425780/1‑149 (MQ=255)
                                                             aCGGGCGCGGGAAAGAGAAGTAATTCATCTTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                        <  1:1197851/149‑1 (MQ=255)
                                                             aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                        >  1:455842/1‑149 (MQ=255)
                                                             aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                        >  1:115644/1‑149 (MQ=255)
                                                                ggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACCTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGAGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaa                                                                                     <  1:356081/149‑1 (MQ=255)
                                                                 gcgcGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaaa                                                                                    <  2:145454/149‑1 (MQ=255)
                                                                    cgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAatta                                                                                 >  2:695337/1‑149 (MQ=255)
                                                                      ggAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAGTTATCGTTCTGATAATAGTGAAattatt                                                                               >  2:1127655/1‑149 (MQ=255)
                                                                       gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                              >  2:1547592/1‑149 (MQ=255)
                                                                        aaaGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCACACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATAAATTATCCTTCTGAAAATAGTGAAATTATTAc                                                                             <  2:1189683/149‑1 (MQ=255)
                                                                           gagaAGTAATTCATACTGTACTGGTACGTTGTACACACCTGTGCCAACGGGATCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGGGCACGTAGAAAGATTAATTCTCCTTCTGAAAATAGTGAAATTCTTACGCa                                                                          <  1:1423674/149‑1 (MQ=38)
                                                                            agaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGGGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAg                                                                         >  1:132134/1‑149 (MQ=255)
                                                                                     tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                >  1:768220/1‑149 (MQ=255)
                                                                                     tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                <  1:973573/149‑1 (MQ=255)
                                                                                     tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCACCTCACACTACCCCTCACCCCAAAGGGTCGAGGGGACCGTTCGTGATCGTAGAAATATTAATTATACTTATGAAAATAGTGAAAGTATTTCGCATTACCgagca                                                                >  1:1013732/1‑145 (MQ=255)
                                                                                       atatTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACa                                                              <  1:360912/149‑1 (MQ=255)
                                                                                            ttACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                         <  2:1145722/148‑1 (MQ=255)
                                                                                             tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                        >  2:285381/1‑149 (MQ=255)
                                                                                             tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                        <  1:970723/149‑1 (MQ=255)
                                                                                                  ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                   <  1:896673/149‑1 (MQ=255)
                                                                                                  ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCGGAAAATAGTGAAATTATTAAGCAGTACCGCCAACAGTCAGGTTATc                                                   >  2:705488/1‑149 (MQ=255)
                                                                                                  ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                   >  2:52526/1‑149 (MQ=255)
                                                                                                   tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                  <  1:157505/149‑1 (MQ=255)
                                                                                                    aCGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                 <  1:1176177/149‑1 (MQ=255)
                                                                                                     cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTTCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                <  1:873513/149‑1 (MQ=255)
                                                                                                     cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                >  2:944769/1‑149 (MQ=255)
                                                                                                      gTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACt                                               >  1:1529794/1‑149 (MQ=255)
                                                                                                       ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCTCGGAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCTACAGTCAGGTTATCGACtt                                              >  1:1276044/1‑149 (MQ=255)
                                                                                                       ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCtt                                              >  1:1054068/1‑149 (MQ=255)
                                                                                                       ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACtt                                              >  2:823441/1‑149 (MQ=255)
                                                                                                         gTACAAACCTGTGCCAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGGTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATATGGAAAGTATTACTCAGTACCGCCAACAGTCAGGTTATCGACTTTc                                            >  2:1062631/1‑149 (MQ=39)
                                                                                                          tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGTAGAAAGAGTAATTATCCTTCTGAAAATTGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                           >  2:549244/1‑149 (MQ=255)
                                                                                                          tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                           >  1:9010/1‑149 (MQ=255)
                                                                                                           aCAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                          >  2:1480134/1‑149 (MQ=255)
                                                                                                               aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                      <  1:1004931/149‑1 (MQ=255)
                                                                                                                ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                     >  1:1405431/1‑149 (MQ=255)
                                                                                                                ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCATTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                     >  1:660331/1‑149 (MQ=255)
                                                                                                                 cTGTGCCAACGGGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtgg                                    <  2:94016/149‑1 (MQ=255)
                                                                                                                     gCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctgg                               <  2:1237011/149‑1 (MQ=255)
                                                                                                                      ccACCGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCACCAGTCAGGTTATCGACGTTCAACGTttgctggc                              >  2:94706/1‑149 (MQ=255)
                                                                                                                      ccAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                              <  1:61680/149‑1 (MQ=255)
                                                                                                                      ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCTTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTAGCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                              <  1:339320/149‑1 (MQ=255)
                                                                                                                      ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                              <  2:150178/149‑1 (MQ=255)
                                                                                                                      ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGCGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                              <  2:1471362/149‑1 (MQ=255)
                                                                                                                       cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                             >  1:837474/1‑149 (MQ=255)
                                                                                                                                ccccTCACCCTCACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAc                    <  2:1124638/149‑1 (MQ=255)
                                                                                                                                  ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAATATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCGTGGGCCAGCCAAcgt                  <  2:341473/149‑3 (MQ=38)
                                                                                                                                  ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAAGTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCTTTCAACGTTGGATGGCCCACGCCCACCg                  >  2:1014064/1‑149 (MQ=255)
                                                                                                                                   cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                 >  1:1263756/1‑149 (MQ=255)
                                                                                                                                      aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGCAAGATTAATTTTCCTTCTGAAAATAGTGAAAGTATTACGCAGTACCGCCAACAGTCAGGTTATCGCATTTAAAACTTTTCTGGGCCACGCGACCGGGCaa              >  2:1463284/1‑149 (MQ=37)
                                                                                                                                      aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCACCCGCCaa              >  2:957913/1‑149 (MQ=255)
                                                                                                                                        ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCAAGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc            >  2:966670/1‑149 (MQ=255)
                                                                                                                                         ggtccccTCGCCCCTTTGGGGTGAGGGTACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACt           >  1:365511/5‑149 (MQ=255)
                                                                                                                                           aaCCCTCACCCCACAGGGGCGAGGGGACCGTGCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTAATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt         <  1:39773/149‑1 (MQ=255)
                                                                                                                                            ccccTCGCCCCTTTGGGGTGAGGGGACCGTTGGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGTAATTATTAGGCAGTACCGCCAACAGTCAGGATAGCGCGTTTCAACGTTGGCTGGCCCACGCCCACCGGCaaccgtag        >  1:1272586/2‑143 (MQ=255)
                                                                                                                                            aCCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg        <  1:754983/149‑1 (MQ=255)
                                                                                                                                            aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg        <  2:350083/149‑1 (MQ=255)
                                                                                                                                            aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg        <  1:1562001/149‑1 (MQ=255)
                                                                                                                                             cccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc       <  2:371565/149‑1 (MQ=255)
                                                                                                                                              ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGcc      <  2:768220/149‑1 (MQ=255)
                                                                                                                                               cTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccc     >  2:45186/1‑149 (MQ=255)
                                                                                                                                                tCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccgt    >  1:836734/1‑147 (MQ=255)
                                                                                                                                                  aCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAACAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTGTGCCcttc  >  1:644633/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTC  >  NC_000913/3390366‑3390660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: