Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,517 A→T 5.5% intergenic (+68/+66) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,5170AT 5.5% 306.5 / 4.1 113intergenic (+68/+66)aaeR/tldDtranscriptional regulator for aaeXAB operon/putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (57/49);  new base T (2/5);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTT  >  NC_000913/3390369‑3390662
                                                                                                                                                    |                                                                                                                                                  
gTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCa                                                                                                                                                    >  2:1004931/1‑149 (MQ=255)
 tGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCCCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCaa                                                                                                                                                   >  1:821679/1‑149 (MQ=255)
 tGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCACGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaa                                                                                                                                                   <  1:1100940/149‑1 (MQ=255)
  gATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaaa                                                                                                                                                  >  1:1237011/1‑149 (MQ=255)
   aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                                 <  2:242860/149‑1 (MQ=255)
      aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                              >  1:1481118/1‑149 (MQ=255)
       aCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGc                                                                                                                                             >  1:44411/1‑149 (MQ=255)
          cGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                          <  1:106533/149‑1 (MQ=255)
           ggtGACTGATTATTTTGTTGAGGGCGGTAAATTGTGTCAGGAGATGCACGGGCGCGGTAAAGAGAATTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                         <  2:1407431/147‑1 (MQ=255)
           gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                         <  2:1221733/149‑1 (MQ=255)
           gCTGACGGATTATTTTGTTGAGCTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                         <  1:258076/149‑1 (MQ=255)
             ttactgATTATTTTGTTGAGGTCTGTAAATTGTTTCAGGAGAGGCACGGGCGCGGGAAAGAGATGTAATTCATATTGTACTGTTACGTTGTACATACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgggg                                                                                                                                       <  1:1485985/144‑1 (MQ=39)
             tGACGGAGTATTTTGTTGAGGACGGCAAATTGTTTCAGGAGATGCCCGGGCGCGGGAAAGAGAAGTAATGCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggt                                                                                                                                       <  1:391190/149‑2 (MQ=255)
                cGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGAcc                                                                                                                                    >  2:896673/1‑149 (MQ=255)
                cGGATCATTTTGTTGAGGTCGGTAAATTGTATCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                    <  2:622457/149‑5 (MQ=255)
                 ggATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCg                                                                                                                                   >  2:650301/1‑149 (MQ=255)
                       ttttGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTg                                                                                                                             >  2:1176177/1‑149 (MQ=255)
                           gttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACg                                                                                                                         >  1:341473/1‑149 (MQ=255)
                               aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGa                                                                                                                     >  2:37483/1‑149 (MQ=255)
                               aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACAGTTCGTGCACGTAGa                                                                                                                     >  1:94016/1‑149 (MQ=255)
                                     gTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGatt                                                                                                               <  2:1545618/149‑1 (MQ=255)
                                      tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGGAGAAAGatta                                                                                                              >  2:315229/1‑149 (MQ=255)
                                      tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGagta                                                                                                              >  1:1452346/1‑149 (MQ=255)
                                       aaaTTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGattaa                                                                                                             >  1:175838/1‑149 (MQ=255)
                                           tctatccggAGCTGCACGCGCGCGGGAACGCGAATGACTTCATATTGTACTGTTACGTTGTACAACCCTGTGCCAAGGGGGTCCCCGCACCCTAACCCACACCCCTAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAt                                                                                                         <  2:1446595/142‑1 (MQ=255)
                                            gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCTTTCGTGCACGTAGAAAGATTAATTATc                                                                                                        <  1:554883/149‑1 (MQ=255)
                                            gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                        <  1:650301/149‑1 (MQ=255)
                                              ttCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCt                                                                                                      >  1:1233044/1‑149 (MQ=255)
                                                cAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTc                                                                                                    <  2:669712/149‑1 (MQ=255)
                                                     gaTGCACGGGCGCGGGAACGAGAAGTAATTCATATTGTACTGTTTCGTTGTACAAACCTGTGCCAACGGGTGCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCGTTCGTGCACGTAGAAGGATAATAATCTTTCtacgtg                                                                                              <  2:1112739/149‑6 (MQ=255)
                                                        gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                            >  1:1306334/1‑149 (MQ=255)
                                                        gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                            <  1:1033424/149‑1 (MQ=255)
                                                         cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                           >  1:350083/1‑149 (MQ=255)
                                                         cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCATCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                           >  2:1425780/1‑149 (MQ=255)
                                                          aCGGGCGCGGGAAAGAGAAGTAATTCATCTTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                          <  1:1197851/149‑1 (MQ=255)
                                                          aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                          >  1:115644/1‑149 (MQ=255)
                                                          aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                          >  1:455842/1‑149 (MQ=255)
                                                             ggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACCTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGAGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaa                                                                                       <  1:356081/149‑1 (MQ=255)
                                                              gcgcGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaaa                                                                                      <  2:145454/149‑1 (MQ=255)
                                                                 cgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAatta                                                                                   >  2:695337/1‑149 (MQ=255)
                                                                   ggAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAGTTATCGTTCTGATAATAGTGAAattatt                                                                                 >  2:1127655/1‑149 (MQ=255)
                                                                    gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                                >  2:1547592/1‑149 (MQ=255)
                                                                     aaaGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCACACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATAAATTATCCTTCTGAAAATAGTGAAATTATTAc                                                                               <  2:1189683/149‑1 (MQ=255)
                                                                        gagaAGTAATTCATACTGTACTGGTACGTTGTACACACCTGTGCCAACGGGATCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGGGCACGTAGAAAGATTAATTCTCCTTCTGAAAATAGTGAAATTCTTACGCa                                                                            <  1:1423674/149‑1 (MQ=38)
                                                                             gTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTAcc                                                                       <  1:321950/149‑1 (MQ=255)
                                                                                  tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                  <  1:973573/149‑1 (MQ=255)
                                                                                  tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCACCTCACACTACCCCTCACCCCAAAGGGTCGAGGGGACCGTTCGTGATCGTAGAAATATTAATTATACTTATGAAAATAGTGAAAGTATTTCGCATTACCgagca                                                                  >  1:1013732/1‑145 (MQ=255)
                                                                                    atatTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACa                                                                <  1:360912/149‑1 (MQ=255)
                                                                                          tACTGTTACGTTGTACAACCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAGCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          >  1:1072068/1‑149 (MQ=255)
                                                                                          tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          >  2:285381/1‑149 (MQ=255)
                                                                                          tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          <  1:970723/149‑1 (MQ=255)
                                                                                               ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     <  2:1481118/149‑1 (MQ=255)
                                                                                               ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     <  1:896673/149‑1 (MQ=255)
                                                                                               ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCGGAAAATAGTGAAATTATTAAGCAGTACCGCCAACAGTCAGGTTATc                                                     >  2:705488/1‑149 (MQ=255)
                                                                                               ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     >  2:52526/1‑149 (MQ=255)
                                                                                                tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                    <  1:1369270/149‑1 (MQ=255)
                                                                                                tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                    <  1:157505/149‑1 (MQ=255)
                                                                                                 aCGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                   <  1:1176177/149‑1 (MQ=255)
                                                                                                  cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTTCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                  <  1:873513/149‑1 (MQ=255)
                                                                                                  cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                  >  2:944769/1‑149 (MQ=255)
                                                                                                    ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCTCGGAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCTACAGTCAGGTTATCGACtt                                                >  1:1276044/1‑149 (MQ=255)
                                                                                                    ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCtt                                                >  1:1054068/1‑149 (MQ=255)
                                                                                                    ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACtt                                                >  2:823441/1‑149 (MQ=255)
                                                                                                      gTACAAACCTGTGCCAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGGTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATATGGAAAGTATTACTCAGTACCGCCAACAGTCAGGTTATCGACTTTc                                              >  2:1062631/1‑149 (MQ=39)
                                                                                                       tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGTAGAAAGAGTAATTATCCTTCTGAAAATTGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                             >  2:549244/1‑149 (MQ=255)
                                                                                                       tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                             >  1:9010/1‑149 (MQ=255)
                                                                                                            aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                        <  1:818208/149‑1 (MQ=255)
                                                                                                            aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                        <  1:1004931/149‑1 (MQ=255)
                                                                                                             ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                       >  1:1405431/1‑149 (MQ=255)
                                                                                                             ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCATTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                       >  1:660331/1‑149 (MQ=255)
                                                                                                              cTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCCCGTAGAAAGATTAATTATCCTTCTGAAAAAAGTGAAATTATTACGCAGTACCGCCAACAGACAGGTTATCGACTTTCAACGTtgg                                      >  1:896466/1‑149 (MQ=255)
                                                                                                              cTGTGCCAACGGGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtgg                                      <  2:94016/149‑1 (MQ=255)
                                                                                                                  gCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctgg                                 <  2:1237011/149‑1 (MQ=255)
                                                                                                                   ccACCGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCACCAGTCAGGTTATCGACGTTCAACGTttgctggc                                >  2:94706/1‑149 (MQ=255)
                                                                                                                   ccAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  1:61680/149‑1 (MQ=255)
                                                                                                                   ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCTTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTAGCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  1:339320/149‑1 (MQ=255)
                                                                                                                   ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  2:150178/149‑1 (MQ=255)
                                                                                                                   ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGCGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  2:1471362/149‑1 (MQ=255)
                                                                                                                    cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                               >  1:837474/1‑149 (MQ=255)
                                                                                                                    cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATAGACTTTCAACGTTGGCTGGcc                               >  1:1536496/1‑149 (MQ=255)
                                                                                                                           ttCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCa                        >  1:371565/1‑149 (MQ=255)
                                                                                                                             ccccTCACCCTCACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAc                      <  2:1124638/149‑1 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAATATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCGTGGGCCAGCCAAcgt                    <  2:341473/149‑3 (MQ=38)
                                                                                                                               ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAAGTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCTTTCAACGTTGGATGGCCCACGCCCACCg                    >  2:1014064/1‑149 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                   >  1:1263756/1‑149 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                   <  2:115644/149‑1 (MQ=255)
                                                                                                                                   aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGCAAGATTAATTTTCCTTCTGAAAATAGTGAAAGTATTACGCAGTACCGCCAACAGTCAGGTTATCGCATTTAAAACTTTTCTGGGCCACGCGACCGGGCaa                >  2:1463284/1‑149 (MQ=37)
                                                                                                                                   aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCACCCGCCaa                >  2:957913/1‑149 (MQ=255)
                                                                                                                                     ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCAAGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc              >  2:966670/1‑149 (MQ=255)
                                                                                                                                        aaCCCTCACCCCACAGGGGCGAGGGGACCGTGCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTAATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt           <  1:39773/149‑1 (MQ=255)
                                                                                                                                         ccccTCGCCCCTTTGGGGTGAGGGGACCGTTGGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGTAATTATTAGGCAGTACCGCCAACAGTCAGGATAGCGCGTTTCAACGTTGGCTGGCCCACGCCCACCGGCaaccgtag          >  1:1272586/2‑143 (MQ=255)
                                                                                                                                         aCCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  1:754983/149‑1 (MQ=255)
                                                                                                                                         aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  1:1562001/149‑1 (MQ=255)
                                                                                                                                         aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  2:350083/149‑1 (MQ=255)
                                                                                                                                          cccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc         <  2:371565/149‑1 (MQ=255)
                                                                                                                                           ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGcc        <  2:768220/149‑1 (MQ=255)
                                                                                                                                            cTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccc       >  2:45186/1‑149 (MQ=255)
                                                                                                                                             tCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccgt      >  1:836734/1‑147 (MQ=255)
                                                                                                                                               aCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAACAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTGTGCCcttc    >  1:644633/1‑149 (MQ=255)
                                                                                                                                                 cccAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCcttctt  >  1:1001876/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTT  >  NC_000913/3390369‑3390662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: