Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,519 A→T 5.1% intergenic (+70/+64) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,5190AT 5.1% 291.2 / 4.2 113intergenic (+70/+64)aaeR/tldDtranscriptional regulator for aaeXAB operon/putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (56/48);  new base T (2/4);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 114 total aligned reads displayed.

GATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTT  >  NC_000913/3390371‑3390662
                                                                                                                                                    |                                                                                                                                                
gATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaaa                                                                                                                                                  >  1:1237011/1‑149 (MQ=255)
 aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                                 <  2:242860/149‑1 (MQ=255)
    aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                              >  1:1481118/1‑149 (MQ=255)
     aCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGc                                                                                                                                             >  1:44411/1‑149 (MQ=255)
        cGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                          <  1:106533/149‑1 (MQ=255)
        cGCTGACGGATTATTTTGTTGAGGGCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                          <  1:529848/149‑1 (MQ=255)
         ggtGACTGATTATTTTGTTGAGGGCGGTAAATTGTGTCAGGAGATGCACGGGCGCGGTAAAGAGAATTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                         <  2:1407431/147‑1 (MQ=255)
         gCTGACGGATTATTTTGTTGAGCTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                         <  1:258076/149‑1 (MQ=255)
          cTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAggg                                                                                                                                        >  1:995100/1‑149 (MQ=255)
              cGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGAcc                                                                                                                                    >  2:896673/1‑149 (MQ=255)
              cGGATCATTTTGTTGAGGTCGGTAAATTGTATCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                    <  2:622457/149‑5 (MQ=255)
               ggATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCg                                                                                                                                   >  2:650301/1‑149 (MQ=255)
                     ttttGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTg                                                                                                                             >  2:1176177/1‑149 (MQ=255)
                         gttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACg                                                                                                                         >  1:341473/1‑149 (MQ=255)
                             aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGa                                                                                                                     >  2:37483/1‑149 (MQ=255)
                             aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACAGTTCGTGCACGTAGa                                                                                                                     >  1:94016/1‑149 (MQ=255)
                                   gTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGatt                                                                                                               <  2:1545618/149‑1 (MQ=255)
                                    tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGagta                                                                                                              >  1:1452346/1‑149 (MQ=255)
                                     aaaTTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGattaa                                                                                                             >  1:175838/1‑149 (MQ=255)
                                         tctatccggAGCTGCACGCGCGCGGGAACGCGAATGACTTCATATTGTACTGTTACGTTGTACAACCCTGTGCCAAGGGGGTCCCCGCACCCTAACCCACACCCCTAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAt                                                                                                         <  2:1446595/142‑1 (MQ=255)
                                          gtctCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAAATCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                        <  1:1233361/146‑1 (MQ=255)
                                          gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCTTTCGTGCACGTAGAAAGATTAATTATc                                                                                                        <  1:554883/149‑1 (MQ=255)
                                            ttCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCt                                                                                                      >  1:1233044/1‑149 (MQ=255)
                                             tCAGGACATGCACGGGCGCGGGAAAGACAAGTAATTCATATTGTACGGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCACAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCtt                                                                                                     <  1:1066578/149‑1 (MQ=255)
                                              cAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTc                                                                                                    <  2:669712/149‑1 (MQ=255)
                                               aGGAGATTCACGGGCGCGGGATAGAGAAGTAATTCATATTGTACTGTTACGTTGTAGAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACGCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCt                                                                                                   <  1:289353/149‑1 (MQ=255)
                                                   gaTGCACGGGCGCGGGAACGAGAAGTAATTCATATTGTACTGTTTCGTTGTACAAACCTGTGCCAACGGGTGCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCGTTCGTGCACGTAGAAGGATAATAATCTTTCtacgtg                                                                                              <  2:1112739/149‑6 (MQ=255)
                                                      gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                            >  1:1306334/1‑149 (MQ=255)
                                                      gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                            <  1:1033424/149‑1 (MQ=255)
                                                       cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                           >  1:350083/1‑149 (MQ=255)
                                                       cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCATCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                           >  2:1425780/1‑149 (MQ=255)
                                                        aCGGGCGCGGGAAAGAGAAGTAATTCATCTTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                          <  1:1197851/149‑1 (MQ=255)
                                                        aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                          >  1:455842/1‑149 (MQ=255)
                                                           ggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACCTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGAGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaa                                                                                       <  1:356081/149‑1 (MQ=255)
                                                            gcgcGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaaa                                                                                      <  2:145454/149‑1 (MQ=255)
                                                               cgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAatta                                                                                   >  2:695337/1‑149 (MQ=255)
                                                                 ggAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAGTTATCGTTCTGATAATAGTGAAattatt                                                                                 >  2:1127655/1‑149 (MQ=255)
                                                                  gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                                >  2:1547592/1‑149 (MQ=255)
                                                                   aaaGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCACACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATAAATTATCCTTCTGAAAATAGTGAAATTATTAc                                                                               <  2:1189683/149‑1 (MQ=255)
                                                                      gagaAGTAATTCATACTGTACTGGTACGTTGTACACACCTGTGCCAACGGGATCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGGGCACGTAGAAAGATTAATTCTCCTTCTGAAAATAGTGAAATTCTTACGCa                                                                            <  1:1423674/149‑1 (MQ=38)
                                                                       agaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGGGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAg                                                                           >  1:132134/1‑149 (MQ=255)
                                                                                tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                  >  1:768220/1‑149 (MQ=255)
                                                                                tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                  <  1:973573/149‑1 (MQ=255)
                                                                                tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                  >  2:935275/1‑149 (MQ=255)
                                                                                tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCACCTCACACTACCCCTCACCCCAAAGGGTCGAGGGGACCGTTCGTGATCGTAGAAATATTAATTATACTTATGAAAATAGTGAAAGTATTTCGCATTACCgagca                                                                  >  1:1013732/1‑145 (MQ=255)
                                                                                  atatTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACa                                                                <  1:360912/149‑1 (MQ=255)
                                                                                   tatTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAg                                                               >  2:276835/1‑149 (MQ=255)
                                                                                       ttACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                           <  2:1145722/148‑1 (MQ=255)
                                                                                        tACTGTTACGTTGTACAACCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAGCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          >  1:1072068/1‑149 (MQ=255)
                                                                                        tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          >  2:285381/1‑149 (MQ=255)
                                                                                        tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                          <  1:970723/149‑1 (MQ=255)
                                                                                             ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     <  1:896673/149‑1 (MQ=255)
                                                                                             ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     <  2:1481118/149‑1 (MQ=255)
                                                                                             ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCGGAAAATAGTGAAATTATTAAGCAGTACCGCCAACAGTCAGGTTATc                                                     >  2:705488/1‑149 (MQ=255)
                                                                                             ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                     >  2:52526/1‑149 (MQ=255)
                                                                                              tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                    <  1:1369270/149‑1 (MQ=255)
                                                                                              tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                    <  1:157505/149‑1 (MQ=255)
                                                                                               aCGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                   <  1:1176177/149‑1 (MQ=255)
                                                                                                cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTTCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                  <  1:873513/149‑1 (MQ=255)
                                                                                                cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                  >  2:944769/1‑149 (MQ=255)
                                                                                                 gTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACt                                                 >  1:1529794/1‑149 (MQ=255)
                                                                                                  ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCtt                                                >  1:1054068/1‑149 (MQ=255)
                                                                                                     tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                             >  1:9010/1‑149 (MQ=255)
                                                                                                          aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                        <  1:818208/149‑1 (MQ=255)
                                                                                                          aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                        <  1:1004931/149‑1 (MQ=255)
                                                                                                           ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                       >  1:1405431/1‑149 (MQ=255)
                                                                                                           ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCATTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                       >  1:660331/1‑149 (MQ=255)
                                                                                                            cTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCCCGTAGAAAGATTAATTATCCTTCTGAAAAAAGTGAAATTATTACGCAGTACCGCCAACAGACAGGTTATCGACTTTCAACGTtgg                                      >  1:896466/1‑149 (MQ=255)
                                                                                                            cTGTGCCAACGGGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtgg                                      <  2:94016/149‑1 (MQ=255)
                                                                                                                gCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctgg                                 <  2:1237011/149‑1 (MQ=255)
                                                                                                                 ccACCGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCACCAGTCAGGTTATCGACGTTCAACGTttgctggc                                >  2:94706/1‑149 (MQ=255)
                                                                                                                 ccAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  1:61680/149‑1 (MQ=255)
                                                                                                                 ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCTTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTAGCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  1:339320/149‑1 (MQ=255)
                                                                                                                 ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  2:150178/149‑1 (MQ=255)
                                                                                                                 ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGCGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                <  2:1471362/149‑1 (MQ=255)
                                                                                                                  cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                               >  1:837474/1‑149 (MQ=255)
                                                                                                                  cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATAGACTTTCAACGTTGGCTGGcc                               >  1:1536496/1‑149 (MQ=255)
                                                                                                                         ttCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCa                        >  1:371565/1‑149 (MQ=255)
                                                                                                                           ccccTCACCCTCACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAc                      <  2:1124638/149‑1 (MQ=255)
                                                                                                                             ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAATATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCGTGGGCCAGCCAAcgt                    <  2:341473/149‑3 (MQ=38)
                                                                                                                             ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAAGTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCTTTCAACGTTGGATGGCCCACGCCCACCg                    >  2:1014064/1‑149 (MQ=255)
                                                                                                                             ccTCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGGTATCGACTTTCACCGTTGGCTGGCCCACGCCAACCg                    >  2:653241/1‑149 (MQ=255)
                                                                                                                              cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                   >  1:1263756/1‑149 (MQ=255)
                                                                                                                              cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                   <  2:44411/149‑1 (MQ=255)
                                                                                                                              cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                   <  2:115644/149‑1 (MQ=255)
                                                                                                                                 aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGCAAGATTAATTTTCCTTCTGAAAATAGTGAAAGTATTACGCAGTACCGCCAACAGTCAGGTTATCGCATTTAAAACTTTTCTGGGCCACGCGACCGGGCaa                >  2:1463284/1‑149 (MQ=37)
                                                                                                                                 aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCACCCGCCaa                >  2:957913/1‑149 (MQ=255)
                                                                                                                                   ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCAAGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc              >  2:966670/1‑149 (MQ=255)
                                                                                                                                    ggtccccTCGCCCCTTTGGGGTGAGGGTACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACt             >  1:365511/5‑149 (MQ=255)
                                                                                                                                      aaCCCTCACCCCACAGGGGCGAGGGGACCGTGCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTAATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt           <  1:39773/149‑1 (MQ=255)
                                                                                                                                       ccccTCGCCCCTTTGGGGTGAGGGGACCGTTGGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGTAATTATTAGGCAGTACCGCCAACAGTCAGGATAGCGCGTTTCAACGTTGGCTGGCCCACGCCCACCGGCaaccgtag          >  1:1272586/2‑143 (MQ=255)
                                                                                                                                       aCCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  1:754983/149‑1 (MQ=255)
                                                                                                                                       aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  1:1562001/149‑1 (MQ=255)
                                                                                                                                       aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg          <  2:350083/149‑1 (MQ=255)
                                                                                                                                        cccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc         <  2:371565/149‑1 (MQ=255)
                                                                                                                                         ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGcc        <  2:768220/149‑1 (MQ=255)
                                                                                                                                          cTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccc       >  2:45186/1‑149 (MQ=255)
                                                                                                                                           tCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccgt      >  1:836734/1‑147 (MQ=255)
                                                                                                                                             aCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAACAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTGTGCCcttc    >  1:644633/1‑149 (MQ=255)
                                                                                                                                               cccAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCcttctt  >  1:1001876/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTT  >  NC_000913/3390371‑3390662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: