Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,524 C→T 5.4% intergenic (+75/+59) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,5240CT 5.4% 315.4 / 6.7 114intergenic (+75/+59)aaeR/tldDtranscriptional regulator for aaeXAB operon/putative peptidase
Reads supporting (aligned to +/- strand):  ref base C (53/52);  new base T (2/4);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 116 total aligned reads displayed.

ACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAG  >  NC_000913/3390376‑3390670
                                                                                                                                                    |                                                                                                                                                   
aCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGc                                                                                                                                                     >  1:44411/1‑149 (MQ=255)
   cGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                                  <  1:106533/149‑1 (MQ=255)
    ggtGACTGATTATTTTGTTGAGGGCGGTAAATTGTGTCAGGAGATGCACGGGCGCGGTAAAGAGAATTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                                 <  2:1407431/147‑1 (MQ=255)
    gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                                 <  2:1221733/149‑1 (MQ=255)
    gCTGACGGATTATTTTGTTGAGCTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                                 <  1:258076/149‑1 (MQ=255)
     cTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAggg                                                                                                                                                >  1:995100/1‑149 (MQ=255)
      ttactgATTATTTTGTTGAGGTCTGTAAATTGTTTCAGGAGAGGCACGGGCGCGGGAAAGAGATGTAATTCATATTGTACTGTTACGTTGTACATACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgggg                                                                                                                                               <  1:1485985/144‑1 (MQ=39)
      tGACGGAGTATTTTGTTGAGGACGGCAAATTGTTTCAGGAGATGCCCGGGCGCGGGAAAGAGAAGTAATGCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggt                                                                                                                                               <  1:391190/149‑2 (MQ=255)
         cGGATCATTTTGTTGAGGTCGGTAAATTGTATCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                            <  2:622457/149‑5 (MQ=255)
                ttttGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTg                                                                                                                                     >  2:1176177/1‑149 (MQ=255)
                    gttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACg                                                                                                                                 >  1:341473/1‑149 (MQ=255)
                        aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGa                                                                                                                             >  2:37483/1‑149 (MQ=255)
                        aGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACAGTTCGTGCACGTAGa                                                                                                                             >  1:94016/1‑149 (MQ=255)
                              gTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGatt                                                                                                                       <  2:1545618/149‑1 (MQ=255)
                               tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGGAGAAAGatta                                                                                                                      >  2:315229/1‑149 (MQ=255)
                                   ttGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCCACGGGTTCCCCTCACCCTAACCCTCACGCCAACGGGGCGAGGGGACCGTTCGTGCCCGTAGAAAGattaatta                                                                                                                  >  1:887320/1‑149 (MQ=255)
                                     gtctCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAAATCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                                <  1:1233361/146‑1 (MQ=255)
                                     gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCTTTCGTGCACGTAGAAAGATTAATTATc                                                                                                                <  1:554883/149‑1 (MQ=255)
                                     gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                                <  1:650301/149‑1 (MQ=255)
                                       ttCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCt                                                                                                              >  1:1233044/1‑149 (MQ=255)
                                        tCAGGACATGCACGGGCGCGGGAAAGACAAGTAATTCATATTGTACGGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCACAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCtt                                                                                                             <  1:1066578/149‑1 (MQ=255)
                                         cAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTc                                                                                                            <  2:669712/149‑1 (MQ=255)
                                          aGGAGATTCACGGGCGCGGGATAGAGAAGTAATTCATATTGTACTGTTACGTTGTAGAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACGCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCt                                                                                                           <  1:289353/149‑1 (MQ=255)
                                              gaTGCACGGGCGCGGGAACGAGAAGTAATTCATATTGTACTGTTTCGTTGTACAAACCTGTGCCAACGGGTGCCCCTCACCCTAACCCTCACCCCAACGGGGCGAGGGGACCGTTCGTGCACGTAGAAGGATAATAATCTTTCtacgtg                                                                                                      <  2:1112739/149‑6 (MQ=255)
                                                 gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                                    >  1:1306334/1‑149 (MQ=255)
                                                 gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                                    <  1:1033424/149‑1 (MQ=255)
                                                  cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                                   >  1:350083/1‑149 (MQ=255)
                                                  cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCATCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAg                                                                                                   >  2:1425780/1‑149 (MQ=255)
                                                   aCGGGCGCGGGAAAGAGAAGTAATTCATCTTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                                  <  1:1197851/149‑1 (MQ=255)
                                                   aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                                  >  1:455842/1‑149 (MQ=255)
                                                   aCGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGt                                                                                                  >  1:115644/1‑149 (MQ=255)
                                                      ggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACCTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGAGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaa                                                                                               <  1:356081/149‑1 (MQ=255)
                                                       gcgcGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaaa                                                                                              <  2:145454/149‑1 (MQ=255)
                                                          cgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAatta                                                                                           >  2:695337/1‑149 (MQ=255)
                                                            ggAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAGTTATCGTTCTGATAATAGTGAAattatt                                                                                         >  2:1127655/1‑149 (MQ=255)
                                                             gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                                        >  2:1547592/1‑149 (MQ=255)
                                                                 gagaAGTAATTCATACTGTACTGGTACGTTGTACACACCTGTGCCAACGGGATCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGGGCACGTAGAAAGATTAATTCTCCTTCTGAAAATAGTGAAATTCTTACGCa                                                                                    <  1:1423674/149‑1 (MQ=38)
                                                                  agaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGGGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAg                                                                                   >  1:132134/1‑149 (MQ=255)
                                                                      gTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTAcc                                                                               <  1:321950/149‑1 (MQ=255)
                                                                           tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                          <  1:973573/149‑1 (MQ=255)
                                                                           tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                          >  2:935275/1‑149 (MQ=255)
                                                                           tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                          >  1:768220/1‑149 (MQ=255)
                                                                           tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCACCTCACACTACCCCTCACCCCAAAGGGTCGAGGGGACCGTTCGTGATCGTAGAAATATTAATTATACTTATGAAAATAGTGAAAGTATTTCGCATTACCgagca                                                                          >  1:1013732/1‑145 (MQ=255)
                                                                              tatTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAg                                                                       >  2:276835/1‑149 (MQ=255)
                                                                                  ttACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                                   <  2:1145722/148‑1 (MQ=255)
                                                                                   tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                                  <  1:970723/149‑1 (MQ=255)
                                                                                   tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                                  >  2:285381/1‑149 (MQ=255)
                                                                                        ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                             <  2:1481118/149‑1 (MQ=255)
                                                                                        ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                             <  1:896673/149‑1 (MQ=255)
                                                                                        ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCGGAAAATAGTGAAATTATTAAGCAGTACCGCCAACAGTCAGGTTATc                                                             >  2:705488/1‑149 (MQ=255)
                                                                                        ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                             >  2:52526/1‑149 (MQ=255)
                                                                                         tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                            <  1:1369270/149‑1 (MQ=255)
                                                                                         tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                            <  1:157505/149‑1 (MQ=255)
                                                                                          aCGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                           <  1:1176177/149‑1 (MQ=255)
                                                                                           cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTTCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                          <  1:873513/149‑1 (MQ=255)
                                                                                           cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGAc                                                          >  2:944769/1‑149 (MQ=255)
                                                                                            gTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACt                                                         >  1:1529794/1‑149 (MQ=255)
                                                                                             ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCTCGGAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCTACAGTCAGGTTATCGACtt                                                        >  1:1276044/1‑149 (MQ=255)
                                                                                             ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCtt                                                        >  1:1054068/1‑149 (MQ=255)
                                                                                             ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACtt                                                        >  2:823441/1‑149 (MQ=255)
                                                                                                tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCGTGCACGTAGAAAGAGTAATTATCCTTCTGAAAATTGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                                     >  2:549244/1‑149 (MQ=255)
                                                                                                tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                                     >  1:9010/1‑149 (MQ=255)
                                                                                                 aCAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                                    >  2:1480134/1‑149 (MQ=255)
                                                                                                     aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                                <  1:1004931/149‑1 (MQ=255)
                                                                                                     aCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGtt                                                <  1:818208/149‑1 (MQ=255)
                                                                                                      ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                               >  1:1405431/1‑149 (MQ=255)
                                                                                                      ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCATTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                               >  1:660331/1‑149 (MQ=255)
                                                                                                       cTGTGCCAACGGGTTCCCCTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtgg                                              <  2:94016/149‑1 (MQ=255)
                                                                                                           gCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctgg                                         <  2:1237011/149‑1 (MQ=255)
                                                                                                            ccACCGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCACCAGTCAGGTTATCGACGTTCAACGTttgctggc                                        >  2:94706/1‑149 (MQ=255)
                                                                                                            ccAACGGGTTCCCCTCACCCTACCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                        <  1:61680/149‑1 (MQ=255)
                                                                                                            ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCTTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTAGCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                        <  1:339320/149‑1 (MQ=255)
                                                                                                            ccAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGCGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctggc                                        <  2:1471362/149‑1 (MQ=255)
                                                                                                             cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                                       >  1:837474/1‑149 (MQ=255)
                                                                                                             cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATAGACTTTCAACGTTGGCTGGcc                                       >  1:1536496/1‑149 (MQ=255)
                                                                                                                    ttCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCATGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCa                                >  1:371565/1‑149 (MQ=255)
                                                                                                                      ccccTCACCCTCACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAc                              <  2:1124638/149‑1 (MQ=255)
                                                                                                                        ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAATATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCGTGGGCCAGCCAAcgt                            <  2:341473/149‑3 (MQ=38)
                                                                                                                        ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAAGTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGCCTTTCAACGTTGGATGGCCCACGCCCACCg                            >  2:1014064/1‑149 (MQ=255)
                                                                                                                        ccTCACCCTAACCCTCACCCCAAAGGGGAGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATACTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGGTATCGACTTTCACCGTTGGCTGGCCCACGCCAACCg                            >  2:653241/1‑149 (MQ=255)
                                                                                                                         cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                           >  1:1263756/1‑149 (MQ=255)
                                                                                                                         cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                           <  2:44411/149‑1 (MQ=255)
                                                                                                                         cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                           <  2:115644/149‑1 (MQ=255)
                                                                                                                            aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGCAAGATTAATTTTCCTTCTGAAAATAGTGAAAGTATTACGCAGTACCGCCAACAGTCAGGTTATCGCATTTAAAACTTTTCTGGGCCACGCGACCGGGCaa                        >  2:1463284/1‑149 (MQ=37)
                                                                                                                            aCCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCACCCGCCaa                        >  2:957913/1‑149 (MQ=255)
                                                                                                                              ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCAAGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc                      >  2:966670/1‑149 (MQ=255)
                                                                                                                               ggtccccTCGCCCCTTTGGGGTGAGGGTACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACt                     >  1:365511/5‑149 (MQ=255)
                                                                                                                                 aaCCCTCACCCCACAGGGGCGAGGGGACCGTGCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGGGAAATTATTACGCAGTACCGCCAACAGTCAGGTAATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt                   <  1:39773/149‑1 (MQ=255)
                                                                                                                                  aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg                  <  1:1562001/149‑1 (MQ=255)
                                                                                                                                  aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg                  <  2:350083/149‑1 (MQ=255)
                                                                                                                                    ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGcc                <  2:768220/149‑1 (MQ=255)
                                                                                                                                     cTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccc               >  2:45186/1‑149 (MQ=255)
                                                                                                                                      tCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccgt              >  1:836734/1‑147 (MQ=255)
                                                                                                                                        aCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAACAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTGTGCCcttc            >  1:644633/1‑149 (MQ=255)
                                                                                                                                          cccAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCcttctt          >  1:1001876/1‑149 (MQ=255)
                                                                                                                                             aatgtgtcGAGGGGAACTATCGTTCACGTATAAAGATTAATTATCCTTCTGAAATTAGTGAAATTATTACTCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTAc       <  2:1452346/142‑1 (MQ=255)
                                                                                                                                                 gggCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCa   <  1:1547592/149‑1 (MQ=255)
                                                                                                                                                 gggCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCa   >  2:1351235/1‑149 (MQ=255)
                                                                                                                                                  ggCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAg  <  1:695337/149‑1 (MQ=255)
                                                                                                                                                  ggCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAg  <  2:1306334/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAG  >  NC_000913/3390376‑3390670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: