Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,293 T→A 6.1% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2930TA 6.1% 307.6 / 3.6 115intergenic (+27/‑82)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/54);  new base A (4/3);  total (58/57)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

ATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTAT  >  NC_000913/3407147‑3407440
                                                                                                                                                  |                                                                                                                                                   
atcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCTCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAttt                                                                                                                                                   >  1:1368551/1‑149 (MQ=255)
atcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAgtt                                                                                                                                                   >  2:174799/1‑149 (MQ=255)
  cGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTc                                                                                                                                                 <  1:1106097/149‑1 (MQ=255)
    aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATACGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCg                                                                                                                                               <  2:381107/149‑1 (MQ=255)
     cGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGACTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCgg                                                                                                                                              >  1:236395/1‑149 (MQ=255)
      ggTATCAAAACCAACGTTGATCTGCAGATCCTCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc                                                                                                                                             <  1:970265/149‑1 (MQ=255)
      ggTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc                                                                                                                                             <  1:852587/149‑1 (MQ=255)
        tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCt                                                                                                                                           >  1:613027/1‑149 (MQ=255)
         aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt                                                                                                                                          <  2:476815/149‑1 (MQ=255)
         aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGAATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt                                                                                                                                          <  2:880348/149‑1 (MQ=255)
          tCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttt                                                                                                                                         <  1:3528/149‑1 (MQ=255)
           cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttt                                                                                                                                        <  2:794216/149‑1 (MQ=255)
           cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGGCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtttt                                                                                                                                        <  1:68750/149‑1 (MQ=255)
            aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                       <  2:1346645/149‑1 (MQ=255)
            aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                       >  1:1533469/1‑149 (MQ=255)
             aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                      >  2:1360429/1‑149 (MQ=255)
              aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgac                                                                                                                                     <  1:1514590/149‑4 (MQ=255)
                 cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                                                                  >  2:575751/1‑149 (MQ=255)
                  aaCGTTGATCTGCAGATCCGCATCATGAATGACGCGAACTTCGAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCTAAAGGACGGATTTTGCGGCCTTTTttatttc                                                                                                                                 >  1:1035636/1‑147 (MQ=255)
                     gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                              <  2:504077/149‑1 (MQ=255)
                     gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                              <  2:558110/149‑1 (MQ=255)
                     gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                              <  2:593709/149‑1 (MQ=255)
                      ttcaTCTGCATATCCGCATAATGAATGACGAGTAATTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGTTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                             <  2:898738/146‑1 (MQ=255)
                          tCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGAATGGTGGCAATAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                         >  2:219045/1‑149 (MQ=255)
                            tGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACCTCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGAGCgc                                                                                                                       >  2:294554/1‑148 (MQ=255)
                             gCAGATCCGCATCATGAATGACGAGTACTTCCAGCATGGTGGAACTAACATCCACTTTCTGTAGAAAAAACTGGGTCTTGTGGAAAAATAAGACTGCTATTGCGTCTAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGat                                                                                                                      <  2:618366/149‑2 (MQ=255)
                             gCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                                      >  1:1024940/1‑149 (MQ=255)
                              cAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGCCa                                                                                                                     >  1:223073/1‑149 (MQ=255)
                                 aTCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAAAGCTGGCGTCAGGAAAAATAAGACTGCTAAAGCTTCAAAATGCAGTAGTTAGCGTACTTTTTTAGTACATGGGATCGCCAGcc                                                                                                                  >  2:285478/1‑149 (MQ=255)
                                    agCATCATTAATCACGAGAACTTCCTGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccccc                                                                                                               <  1:1564933/148‑1 (MQ=255)
                                     gCATCATGAATGCCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGAGCGACAACCCCCa                                                                                                              >  1:1072883/1‑149 (MQ=255)
                                       atcatGAATGACGAGAACTTCCAGCATGGTGGCACTAAAATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTGTTTTATTACTGGGGATCGACAACCCCCATa                                                                                                            >  2:890129/1‑149 (MQ=255)
                                        tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATaa                                                                                                           <  2:1519403/149‑1 (MQ=255)
                                         catGAATGACGAGAACTTCCAGCTTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCAACAACCCCCATAAg                                                                                                          <  2:1113886/149‑1 (MQ=255)
                                           tgactgacGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                        <  1:1203960/149‑1 (MQ=255)
                                           tgaatgaCGAGAACTTCCAGCATTGTGGCACTAACATCCACTATCTGGAGAAATAACTCGGTCTTCAGGAAAAATTAGACTGCTAAATCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                        <  1:466919/149‑1 (MQ=255)
                                            taattaCGAGAAGTTCCAGCATGGTGGCACTAACTTCATCTATCTGGAGAAAAAACTCGTTCTTCAGGAAAAATAAGACTGCTATAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTa                                                                                                       <  2:1087363/148‑1 (MQ=255)
                                             aatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                                                                      >  1:18079/1‑149 (MQ=255)
                                                gaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAAt                                                                                                   <  2:236395/149‑1 (MQ=255)
                                                gaCGAGAACTTCCAGCATGGTGGCACTAACATCCACAATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAACACTGCTAAATCGTCAAAAGGCCGGATTTTCCGTCCTTTTTTATTACTGGGGATAGACAACCACCATAAGGTACaaa                                                                                                   >  1:150081/1‑148 (MQ=255)
                                                  cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACACCCCCCATAAGGTACAATca                                                                                                 >  1:1395759/1‑148 (MQ=255)
                                                  cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATcc                                                                                                 <  2:1215601/149‑1 (MQ=255)
                                                  cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTACAGGAAAAATAAGACTGCTAAAGCGTCAAAAGCCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATcc                                                                                                 >  2:1480241/1‑149 (MQ=255)
                                                   gagaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGCCAACCCCCATAAGGTACAATccc                                                                                                >  2:477793/1‑149 (MQ=255)
                                                   gagaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                                                                >  1:601039/1‑149 (MQ=255)
                                                   gagaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                                                                >  1:993003/1‑149 (MQ=255)
                                                    agaCCTTCCAGCATGGTGGCACTCACATCCACTATCTGGCGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACACCCCCCATAAGGTACAATcccc                                                                                               <  2:845800/149‑1 (MQ=255)
                                                     gaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                                                                              >  1:190735/1‑149 (MQ=255)
                                                         ttCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTACAGCGACAAAAGGCCGGCTTTCCGGCCTTTTTTATTACTGGGGATCGGCACCCCCCATAAGGTTCAATCCCCGCttgc                                                                                         >  2:866286/1‑147 (MQ=255)
                                                          tCCAGAATTGTTGCACTAACATCCACTATCTGGTGAAAAAACTCTTTCTTAATGAAAAATAAGACTGATAAAGCGGCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACGCCCATAAGGTACAATCCCCGCTttc                                                                                         <  2:375957/149‑1 (MQ=255)
                                                           ccAGCATGGTGGCACTAACATCCACTATCGGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACCACCCCCATAAGGTACAAGCCCCGCTttcg                                                                                        >  1:1130263/1‑148 (MQ=255)
                                                            cAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACCACCCCCATAAGGTACAATCCCCGCTttctt                                                                                       >  1:1360216/1‑149 (MQ=255)
                                                              gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGGCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                                                                     >  1:807578/1‑149 (MQ=255)
                                                              gCATGGTGGCAATAACATCCACTATCTGGAGAAAATACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCGTTTTTTATTACTGGTGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                                                                     <  2:81458/149‑1 (MQ=255)
                                                                aTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAccc                                                                                  <  1:42223/149‑1 (MQ=255)
                                                                      gCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAg                                                                             >  1:960898/1‑149 (MQ=255)
                                                                      gCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAg                                                                             >  1:590071/1‑149 (MQ=255)
                                                                        aCTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                           >  2:589371/1‑149 (MQ=255)
                                                                        aCTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGGACAATCCCCGCTTTCTTCACCCATCAggg                                                                           <  2:1049159/149‑1 (MQ=255)
                                                                         cTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa                                                                          <  1:1146727/149‑1 (MQ=255)
                                                                            aCATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaa                                                                       >  1:599572/1‑149 (MQ=255)
                                                                                ccACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAACGCGTCAAAAGGCAGGATGTTCCGGCCTATTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCTCCCATCAGGGACaaaaaa                                                                   >  1:1082031/1‑149 (MQ=255)
                                                                                 cacaATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAAt                                                                  <  1:1096960/149‑1 (MQ=255)
                                                                                   cTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                                                                <  1:457827/149‑1 (MQ=255)
                                                                                     atttGGTGAATAAACTCGTTATTCAGTAATAATAAGATTGATAATTCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCTACAACCCCCATAAGTTACAATCCCCGCTTTCTTCACCCATCACGTACAAAAAATGGac                                                              <  2:144120/146‑1 (MQ=255)
                                                                                     aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTTACGCGTCAAAAGGCCGGAGATTCCGGCCTTGTTTATTACTGTGGATCGACAACCCCCATAAGGTACAATCCCAGCATTCTTCACCACTCAGGGACAAAAAATGGac                                                              >  2:900125/1‑149 (MQ=255)
                                                                                             aaaaaaCTCGGTCTTCAGGAAAAATAAGTCTGCTAAAGCGTCAAAAGGCCGGAGTTTCCGGCCTTTTTTATTACTGGGGATCGAAAACCCACATAATGTACAATCCCCGATTTCTTCACCCATCAGGGACAAAAAATCGACAGTCGttt                                                      >  1:1022367/1‑149 (MQ=255)
                                                                                              aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt                                                     <  2:577734/149‑1 (MQ=255)
                                                                                              aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt                                                     <  2:327705/149‑1 (MQ=255)
                                                                                               aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                    >  2:677257/1‑149 (MQ=255)
                                                                                               aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                    >  2:1387337/1‑149 (MQ=255)
                                                                                                 aaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGtt                                                  <  2:647795/149‑1 (MQ=255)
                                                                                                   cTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCAGGATTTTCCGGCCGTTTTTATTACTGGGGATCGACAACCCCCATTAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa                                                <  1:1036235/149‑1 (MQ=255)
                                                                                                          ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCAt                                         >  1:672312/1‑149 (MQ=255)
                                                                                                          ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTACAGGccctt                                         >  1:666458/1‑147 (MQ=255)
                                                                                                          ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACCGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCAt                                         <  2:213709/149‑1 (MQ=255)
                                                                                                           tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCGTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCGGGCCCATa                                        >  1:858608/1‑149 (MQ=255)
                                                                                                              ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACCATCCCCGCTTTCTTCACCCAGCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAag                                     >  1:1196978/1‑149 (MQ=255)
                                                                                                              cgAAAAAAAAGACAGCCAAACCGTCAAAAGCCCCTATTTTCCGGCCTTTTTTATTACTGGGCAGCGACAACCCCCATAAGGTACAATCCCCGCTTTCTACACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAag                                     <  2:1035636/148‑1 (MQ=255)
                                                                                                               gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTGGTTTTGATCAGGccctataaga                                    >  2:179429/1‑142 (MQ=255)
                                                                                                               gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                    >  2:340212/1‑149 (MQ=255)
                                                                                                               gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCAGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAAAATCCCCGATTTCTTCAAACATCAGGGACAAAAAATGGACAATCGTTTTGTTCAGGCCAATaaaca                                    >  2:811971/1‑147 (MQ=255)
                                                                                                                taaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAagag                                   <  2:1370233/148‑1 (MQ=255)
                                                                                                                aaaaaTAATTCTGCTAAAGCGTCAAAAGGCGTGTTTTTCCGGACTTTTTTATTACTGGTGATCTACTACACCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTTTTGTTCAGGCCCATAAagag                                   <  2:150081/149‑1 (MQ=255)
                                                                                                                     tAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgcg                              >  2:436449/1‑149 (MQ=255)
                                                                                                                        gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctg                           >  1:586939/1‑149 (MQ=255)
                                                                                                                        gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctg                           <  1:1360429/149‑1 (MQ=255)
                                                                                                                          cTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctggg                         >  2:895548/1‑149 (MQ=255)
                                                                                                                             cTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAAACCCCGGTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgc                      >  1:757914/1‑149 (MQ=255)
                                                                                                                               aaaGCGTCAAAAGGCCGGAATTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTGTTGTTCAGGCCCATAAAGAGGAgcgctgggcgctg                    >  1:574665/1‑149 (MQ=255)
                                                                                                                                       aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTgcc            >  2:508771/1‑147 (MQ=255)
                                                                                                                                       aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAc            >  2:91552/1‑149 (MQ=255)
                                                                                                                                          aGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACCATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCt         >  2:330485/1‑149 (MQ=255)
                                                                                                                                           ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtt        <  1:1472001/149‑1 (MQ=255)
                                                                                                                                            gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGAAACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGCCCCtat       >  1:1122602/1‑147 (MQ=255)
                                                                                                                                             ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCTTCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt      >  1:902447/1‑149 (MQ=255)
                                                                                                                                             ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt      >  1:4324/1‑149 (MQ=255)
                                                                                                                                             ccGGATTTTCAGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt      <  2:627875/149‑1 (MQ=255)
                                                                                                                                              cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGATTTCTTCACCAATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCGATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCATTTTg     >  1:221238/1‑149 (MQ=255)
                                                                                                                                                 tttttCCGGCATTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCGGCGCTTTGTTCACCCATGAGGGACAAAAAATGGACACTCGTTTTGTTCAGGGCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTAt  <  2:611005/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTAT  >  NC_000913/3407147‑3407440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: