Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,540 A→G 7.5% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,5400AG 7.5% 293.0 / 10.4 105noncoding (3/120 nt)rrfF5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base A (20/76);  new base G (2/6);  total (22/83)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAC  >  NC_000913/3423392‑3423686
                                                                                                                                                    |                                                                                                                                                  
aGTCTTCAGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGa                                                                                                                                                    <  1:1046378/149‑1 (MQ=38)
   cTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCAACTCTCGCAGGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                                                                 <  2:332549/149‑1 (MQ=255)
         aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCTTGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtt                                                                                                                                           <  1:1349982/149‑1 (MQ=38)
         aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCtg                                                                                                                                           >  1:507580/1‑148 (MQ=14)
         aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACGACCATCGGCGCTACGGCGTGTCACTTCTGAGTTCGCCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAGTCtt                                                                                                                                           >  2:857764/1‑149 (MQ=255)
         aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtt                                                                                                                                           <  1:1293780/149‑1 (MQ=38)
            gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                                                                        <  2:758555/149‑1 (MQ=40)
            gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                                                                        <  1:31839/149‑1 (MQ=40)
            gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                                                                        <  1:870431/149‑1 (MQ=255)
             aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGCGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                                                                                                                                       <  1:358692/149‑1 (MQ=255)
                    tGTTTAATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGc                                                                                                                                <  2:899878/149‑1 (MQ=255)
                        ttATTTGATGTCTGGCAGTTCCCTACTCTGTCATGGGGAGACCCCACACTACGATCGGCGCTTCTGCGATTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa                                                                                                                            <  1:1134458/149‑1 (MQ=14)
                         tATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAACATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCAGACGAAc                                                                                                                           >  1:863770/1‑149 (MQ=255)
                            ttGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                                                                                                                        <  2:768219/149‑1 (MQ=255)
                             tGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                                                                                                                       <  2:243156/149‑1 (MQ=255)
                               aTGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc                                                                                                                     <  1:1429533/149‑1 (MQ=255)
                               aTGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc                                                                                                                     <  1:459365/149‑1 (MQ=255)
                                tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGCACTTTAAcc                                                                                                                    >  1:64184/1‑149 (MQ=255)
                                    tGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACTGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAtcagaacgcagaagcggtctga                                                                                                                <  1:873486‑M1/149‑23 (MQ=255)
                                    tGGCAGTTCCCTACTCTCGAATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaaa                                                                                                                <  1:754708/149‑1 (MQ=255)
                                      gCAGTTCCCTTCTCTCGCCTGGGGCGACCCCACACTACCCTCGGCGCTTCGGCGTGTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAATTTCTTTTCTAATCTGCCGAACTTTAACCTaaaaa                                                                                                              <  1:1316801/149‑1 (MQ=17)
                                      gCAGTTCCCTCCTCTCGCTTGGGGAGACCCCACAGTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaaaaa                                                                                                              <  1:1311035/149‑1 (MQ=255)
                                      gCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTAtcagaacgcagaagcggtctgata                                                                                                              <  1:1123165‑M1/149‑25 (MQ=255)
                                      gCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaaaaa                                                                                                              <  1:10681/149‑1 (MQ=255)
                                      gCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGGGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTAtcagaacgcagaagcggtctgata                                                                                                              <  2:641436‑M1/149‑25 (MQ=255)
                                      gCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaaaaa                                                                                                              <  2:817617/149‑1 (MQ=255)
                                       cAGTTCCCTACGCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAg                                                                                                             <  1:226337/149‑1 (MQ=255)
                                        aGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgt                                                                                                            <  1:1397548/149‑1 (MQ=255)
                                         gtgcccTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAACGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                                                           <  2:1310988/146‑1 (MQ=255)
                                         ggtCCCTACTCTCGCAGGGGGAGACCCCACACTCCCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                                                           <  1:1008641/147‑1 (MQ=255)
                                         gTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                                                           <  1:25697/149‑1 (MQ=255)
                                         gTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                                                           <  2:1562896/149‑1 (MQ=255)
                                         gTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAAGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAACgtg                                                                                                           <  2:855272/149‑1 (MQ=255)
                                          ttCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCCCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg                                                                                                          <  2:1209252/149‑1 (MQ=255)
                                           tCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTAtcagaacgcagaagcggtctgataaaaca                                                                                                         <  1:473784‑M1/149‑30 (MQ=255)
                                             ccTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTACCCTAAAAAGTGGTGc                                                                                                       >  1:399860/1‑149 (MQ=255)
                                             ccTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGc                                                                                                       >  1:26225/1‑149 (MQ=255)
                                              cTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAGGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCt                                                                                                      <  2:920820/149‑1 (MQ=255)
                                              cTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCt                                                                                                      <  1:176030/149‑1 (MQ=255)
                                               tACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTg                                                                                                     <  1:660838/149‑1 (MQ=255)
                                                aCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGa                                                                                                    <  1:105312/149‑1 (MQ=255)
                                                 ctctCGCATGGGGAGACCCCACACTATCATCTGCGCTACGTCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTTGGACGACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGAt                                                                                                   <  1:857764/149‑1 (MQ=18)
                                                  tctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa                                                                                                  >  2:832375/1‑149 (MQ=255)
                                                  tctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa                                                                                                  >  1:1265345/1‑149 (MQ=255)
                                                  tctcGCATGGGGAGACCCCACACTACCATCGGCGCGACGGCGTTTCACTTCTGAGGTCGGCATGGGGGCAGGGGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTTAAAAGTGGTGCTGATa                                                                                                  <  2:1067702/149‑1 (MQ=18)
                                                      gCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa                                                                                              <  2:1421427/149‑1 (MQ=255)
                                                      gCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa                                                                                              <  2:743033/149‑1 (MQ=255)
                                                      gCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTGCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa                                                                                              <  1:1020312/149‑1 (MQ=255)
                                                      gCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTGCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa                                                                                              <  2:1200921/149‑1 (MQ=255)
                                                       cATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCag                                                                                             <  2:12801/149‑1 (MQ=255)
                                                         tGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                                                                           <  1:53501/149‑1 (MQ=255)
                                                           gggTGACCCCACACTACCCTGGGCGCTACGGCGTTTCACTTCTGAGTTCTGCTTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACGCAGAGTc                                                                                         <  2:863770/149‑1 (MQ=21)
                                                           gggAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTc                                                                                         <  2:1242969/149‑1 (MQ=255)
                                                                   ccACAGTACCATCGGCGCTAGGGAGTTTCACTTCTGAGGTCGGCATGGGGTGAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTggg                                                                                 <  1:685728/149‑1 (MQ=25)
                                                                   ccACACTACCATCGGCTCTACGGAGTTTCACTTCTGTGTTCGGCATGGGGTCAGTTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTggg                                                                                 <  2:991617/149‑1 (MQ=255)
                                                                                gtcgcTAAGGCGGTTCACTTCTGAGTACGGCAGGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTGTTTTCTAATCTGCCGAAATTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTa                                                                    <  2:1022057/147‑1 (MQ=37)
                                                                                ggCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTa                                                                    <  2:499343/149‑1 (MQ=255)
                                                                                 gcgcTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTAc                                                                   <  1:1427739/149‑1 (MQ=255)
                                                                                      aCGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                              <  1:996643/149‑1 (MQ=255)
                                                                                      aCGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                              <  1:213078/149‑1 (MQ=255)
                                                                                      aCGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                              <  2:49672/149‑1 (MQ=255)
                                                                                      aCGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                              <  1:244375/149‑1 (MQ=255)
                                                                                       cGGCGTTTCACTTCTGAGTTCGGCATGGGGTCTGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCAGCCTAACTTTAACCTAAAAAGTGGTGCTGATCCCCAGAGTCGAACTGGGGACCTCACCCTTACCTAggg                                                             <  2:1532662/149‑1 (MQ=37)
                                                                                           gtgtCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgag                                                         <  2:739472/146‑3 (MQ=255)
                                                                                           ggttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTGTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcg                                                         <  1:1131245/147‑1 (MQ=255)
                                                                                           gTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCGAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgag                                                         <  2:511774/149‑3 (MQ=255)
                                                                                              tCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACACATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGCACTGGGGACCTCACCCTTACCAAGGGTGCGctc                                                      <  2:399860/149‑1 (MQ=255)
                                                                                               cACTTCTGAGTTGGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGctct                                                     <  2:751343/149‑1 (MQ=255)
                                                                                                aCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTa                                                    <  1:3302/149‑1 (MQ=255)
                                                                                                 catCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGTGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                   <  1:201358/147‑1 (MQ=255)
                                                                                                 cTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGCGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                   <  1:99377/149‑1 (MQ=255)
                                                                                                  ttCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAtcagaacgcagaagcggtctgataaaa                                                                                                           <  1:99991‑M1/92‑28 (MQ=255)
                                                                                                        gTTCGGCATGGTGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACATCACCCTTACCAAGGGTGCGCTCTACCAACTGa                                            <  1:516659/149‑1 (MQ=255)
                                                                                                         ttCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                           >  2:1276020/1‑149 (MQ=255)
                                                                                                         ttCGGCATGGGGTCAGGGGGGACCACCGCGCTACTGCCGCCATACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                           <  2:665244/149‑1 (MQ=255)
                                                                                                          tCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                          <  2:462083/149‑1 (MQ=255)
                                                                                                           cGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                         <  2:64184/149‑1 (MQ=255)
                                                                                                                tgggtgcAGGTGGGACCACCGCGCTACTTCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATc                                    <  2:971654/143‑1 (MQ=255)
                                                                                                                tGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                         <  2:69571/144‑1 (MQ=255)
                                                                                                                tGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                         <  2:63824/144‑1 (MQ=255)
                                                                                                                tGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                         >  1:63824/1‑144 (MQ=255)
                                                                                                                tGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                         >  1:69571/1‑144 (MQ=255)
                                                                                                                 ggggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                   <  2:597890/149‑1 (MQ=255)
                                                                                                                             gACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAtt                       >  1:1485620/1‑149 (MQ=255)
                                                                                                                               ccaccGCGCTACTGCCGCCAGACAAATTCTTTTATATACTACCGCTCTTTCGGCTATATTGTGGAGCTGTTACCCAGAGTCTCTCTGGGGAGCTGCCCATTACCATGGGTGCGCTATAGGAAATGCGGCATAGCAGATAGCTAAAtgtt                     >  2:1363195/1‑146 (MQ=255)
                                                                                                                                caccGCGGTACTGCCGCCAGACATATTCTTTTCTAATGTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAAGTGGGGACCTCACCCTTACCAAGGGTGAGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                    <  2:470251/149‑1 (MQ=37)
                                                                                                                                caccGCGCTCCTGCCGCCATCCAAATTATTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                    <  2:290694/149‑1 (MQ=255)
                                                                                                                                     cgcTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCt               <  2:1265345/149‑1 (MQ=255)
                                                                                                                                        tACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg             <  1:1215411/148‑1 (MQ=255)
                                                                                                                                        tACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg             >  2:1215411/1‑148 (MQ=255)
                                                                                                                                         aCTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCa           <  1:427947/149‑1 (MQ=255)
                                                                                                                                         aCTGCCGCCAGACAAATTCTTTTCTAATATGCCGAACTTTAACATAAAAAGTGGTGCTGAAACCCAGAGTAGATCTGGGGAGATCAACCTTTCCAAGGGTGCGCTCTACCAACTGCGCCATATCAGCACGGTAAATTTGATGGCGGGcc           >  2:1156800/1‑148 (MQ=37)
                                                                                                                                          cTGCCGCCAGACAAATTCTTTTCTAATCTGCCGACCTTTAACCTTAAAAGTGGTGCTGATACCCCGAGGCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCACTCCCAACTGAGCCATTTCAGAGAGCTAAATTTGATGGCTGGCAg          >  2:128325/1‑149 (MQ=37)
                                                                                                                                          cTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg          >  1:517035/1‑149 (MQ=255)
                                                                                                                                           tGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCCGCCCGCTAAATTTGATGCCTGGCAGt         >  1:531535/1‑149 (MQ=255)
                                                                                                                                             ccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCACCTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc       <  1:341038/149‑1 (MQ=255)
                                                                                                                                             ccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc       >  1:77090/1‑149 (MQ=255)
                                                                                                                                              cgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTcc      <  2:26225/149‑1 (MQ=255)
                                                                                                                                                  aGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTTATGCCTGGCAGTTCACTAc  >  2:920702/1‑149 (MQ=255)
                                                                                                                                                  aGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCGCTACCAACTGCGCCATATCAGCACGCTAAATTTGAAGCCTGGCAGTTCCCTAc  >  1:465078/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAC  >  NC_000913/3423392‑3423686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: