Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,469,041 A→G 5.9% S271S (AGT→AGC chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,469,0410AG 5.9% 288.2 / 3.1 100S271S (AGT→AGCchiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base A (44/49);  new base G (3/3);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACT  >  NC_000913/3468893‑3469189
                                                                                                                                                    |                                                                                                                                                    
ccGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGACATGGGTTa                                                                                                                                                      >  2:911838/1‑149 (MQ=255)
 cGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTAc                                                                                                                                                     <  1:1241086/149‑1 (MQ=255)
  ggAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACt                                                                                                                                                    <  1:760666/149‑1 (MQ=255)
  ggAGTTGGGGGCACCGGTGTTTACTGAGTTGGATCAACCGTAGTCACAGCACCGCCATAATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACt                                                                                                                                                    <  1:648190/149‑1 (MQ=255)
   gAGTTGGGGTCACCTGTGTTTACGGAGTGGGATCAACCGGAGTCACAGCACCGCCATTTTTATCCGGGTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg                                                                                                                                                   <  1:1045577/149‑1 (MQ=255)
   gAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCACCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGTACAGGAGCCAGGGTTACCATACTGGCGGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTAGTg                                                                                                                                                   >  1:992241/1‑149 (MQ=255)
    aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg                                                                                                                                                  <  2:350900/149‑1 (MQ=255)
           gTCACCGGTGTTTCCGGAGTTGGGTCACCCGGAGGCACAGCACCGCCATTTTTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATTCTGGCTGAGTTCTGTTTCCTTTGCCGAGCGACCATAACGGCAGGGGGTACTGGCCTCGtt                                                                                                                                           >  2:1473955/1‑149 (MQ=255)
           gTCACCGGTGTTTCCGGAGTTGGCTCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCGAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                           <  1:131076/149‑1 (MQ=255)
           gTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGTCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                           >  2:1103576/1‑149 (MQ=255)
           gTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                           >  1:1470870/1‑149 (MQ=255)
            tCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttt                                                                                                                                          <  2:779544/149‑1 (MQ=255)
             cACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttt                                                                                                                                         <  1:1295847/149‑1 (MQ=255)
               ccGCTGATTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCACTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct                                                                                                                                       <  2:1557484/149‑1 (MQ=255)
                cGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt                                                                                                                                      >  2:52546/1‑149 (MQ=255)
                cGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTAACTGGCATCGTtttctt                                                                                                                                      >  2:90983/1‑149 (MQ=255)
                cGGTGGTTCCGGAGTTGGATCAACCGGAGGCACAGCACCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt                                                                                                                                      <  1:542817/149‑1 (MQ=255)
                 ggTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttcttt                                                                                                                                     <  1:1320362/149‑1 (MQ=255)
                   tgtTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCGAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg                                                                                                                                   <  1:799538/149‑1 (MQ=255)
                       tCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc                                                                                                                               >  2:1007066/1‑149 (MQ=255)
                        caggAGTAGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGCACATAACTCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg                                                                                                                              >  2:1286271/3‑149 (MQ=255)
                         cGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCACCGTTTTCTTTCGCATCGc                                                                                                                             >  2:1559308/1‑149 (MQ=255)
                          ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                            >  2:381106/1‑149 (MQ=255)
                          ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                            <  2:1553350/149‑1 (MQ=255)
                             gTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg                                                                                                                         <  2:1230757/149‑1 (MQ=255)
                              ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGCGCCAGGGTTACCATACTGACTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGTTTACTGGCATCGTTTTCTTTAGAATCGACTagg                                                                                                                        >  2:1447068/1‑149 (MQ=255)
                              ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGACAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGCACATAACGCCAAGGGTTACTGGCATCGTTTTCTTTCGCAACGCCTggg                                                                                                                        >  1:20704/1‑149 (MQ=255)
                                ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa                                                                                                                      >  2:10790/1‑149 (MQ=255)
                                ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAAAGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTGTCTTTCGCATCCCAAGGGCa                                                                                                                      >  2:10761/1‑149 (MQ=39)
                                 gATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGGTTTCTTTCGCATCGCCAGGCCaa                                                                                                                     >  2:502659/1‑149 (MQ=255)
                                      aCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGc                                                                                                                >  2:1355276/1‑149 (MQ=255)
                                          gAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGATGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGATTTATTTCGCATCGCCTGGGCAATTTGCcgcg                                                                                                            >  1:1250181/1‑149 (MQ=255)
                                             tCACAGCCCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCACGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATTGGTTACAGGCATCGTTATCTTTCGCATCGCCTGGGCAATTTGACGCGCCt                                                                                                         >  2:103610/1‑149 (MQ=255)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGttagaaga                                                                                                             >  1:687059/1‑137 (MQ=18)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGttagaaga                                                                                                             <  2:687059/143‑7 (MQ=18)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                       >  2:1422814/1‑149 (MQ=255)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                       >  2:262334/1‑149 (MQ=255)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                       >  2:470537/1‑149 (MQ=255)
                                               acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                       >  2:495853/1‑149 (MQ=255)
                                                  gCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcccc                                                                                                    >  2:560374/1‑148 (MQ=255)
                                                  gCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                    >  2:1296819/1‑149 (MQ=255)
                                                   cACCTCCATTATTCTCTGGCTTAACGGAACATTAGCAAGTTTTACCTTAATGGCTGCTTTCTGTTGGCGTTTCAGCGTGAACTTAACGCCATGGGTTACTGGCATCTTTTTCTTTCGCATAGCATGTTCAATTTGCCGCGCCTACccac                                                                                                   <  2:321064/149‑1 (MQ=255)
                                                       gCCATTAATATCCGGCTTAACGGAACAGGAGCAAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTCCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTGTGTTTCGCATCGCCAGGGAAATTTGCCGCGCCTACCCACCAgg                                                                                               >  2:1224114/1‑149 (MQ=255)
                                                         cATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCTTCGCCTGGGCAAGTTGCCGCGCCTACCCACCAGGCg                                                                                             >  1:1259948/1‑149 (MQ=255)
                                                         cATTATTATCCGGATTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg                                                                                             <  2:708930/149‑1 (MQ=255)
                                                           ttattaTCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt                                                                                           <  2:1139082/149‑1 (MQ=255)
                                                             attatCCGGCTTCACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt                                                                                         <  2:677395/149‑1 (MQ=255)
                                                              ttatCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCGGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt                                                                                        <  2:325869/149‑1 (MQ=255)
                                                                 tCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCAGGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGACGACCCCCCAGGCGTTCTtata                                                                                     >  2:249656/1‑149 (MQ=255)
                                                                 acccccgtAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATATCTGTTGCCGTTGCCTCGCGAACATAACCCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCCGGCGTTCTtata                                                                                     <  2:170015/142‑1 (MQ=255)
                                                                   cGGCTTAACGGAACAGGAGCCAGGGTTTCCATACTGGCTGATTTCTGTTGCCGTTGCAGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa                                                                                   <  2:183802/149‑1 (MQ=255)
                                                                    ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTatatat                                                                                  >  2:383906/1‑149 (MQ=255)
                                                                    ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt                                                                                  >  1:1093104/1‑149 (MQ=255)
                                                                      cTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgc                                                                                <  1:1291345/149‑1 (MQ=255)
                                                                         aaCGGAACAGGAGCCAGGGTAACCATACTGGATGATTTCTGTTCCCGTTGCCGCGCGAACATAACGCCATGGGTTACAGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTAACCACCAGGCGTTCTTATAAATgccgc                                                                             >  2:268865/1‑149 (MQ=38)
                                                                          aCGGAACAGGAGACAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc                                                                            <  1:756669/149‑1 (MQ=255)
                                                                                 aGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCGTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg                                                                     <  2:560768/149‑1 (MQ=255)
                                                                                   gAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCCTCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGaa                                                                   >  1:335753/1‑148 (MQ=255)
                                                                                     gCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATcac                                                                                                                             <  1:920435/89‑3 (MQ=14)
                                                                                        aGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAttgc                                                                                                                                         >  1:88316/1‑72 (MQ=21)
                                                                                        aGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAttgc                                                                                                                                         <  2:88316/74‑3 (MQ=21)
                                                                                         gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGTGTTTCTGGCATAGTTTTCTTTCGCATCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGGTATAGATAACGTa                                                             >  2:1011299/1‑149 (MQ=255)
                                                                                          ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCAGCGCGAACATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCTGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                            <  2:717997/149‑1 (MQ=255)
                                                                                          ggTGACCATTCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGGTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                            <  2:1181223/149‑1 (MQ=255)
                                                                                              aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAtt                                                        >  2:1278188/1‑148 (MQ=255)
                                                                                              aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGGGCTTATAATTGCCGCCGTTCTAGATAACGTACCATg                                                        >  1:871392/1‑149 (MQ=255)
                                                                                               ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATTACGTACCATGt                                                       >  1:865622/1‑149 (MQ=255)
                                                                                               ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt                                                       <  1:16099/149‑1 (MQ=255)
                                                                                                cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                      <  1:176829/149‑1 (MQ=255)
                                                                                                cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGCTACTGGCATCGATTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                      <  1:1473955/149‑1 (MQ=255)
                                                                                                      ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa                                                <  1:1285611/149‑1 (MQ=255)
                                                                                                       gCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAc                                               <  1:1217424/149‑1 (MQ=255)
                                                                                                        cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGGAGAAGAAcc                                              >  2:199757/1‑149 (MQ=255)
                                                                                                         tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                             >  1:740448/1‑149 (MQ=255)
                                                                                                         tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAGGTAGAAGAACCt                                             >  1:436974/1‑149 (MQ=255)
                                                                                                           aTTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTc                                           <  1:381106/149‑1 (MQ=255)
                                                                                                            tttCTTTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTCGCAACGTTTTCTTACGCATCGCCTCGGCAATTTTCCGCGCCTACACACCACGCGTACTTATAAATGCCGCCGTAATAGATAACGTACAATATAGAAGAACCTTcc                                          >  2:407752/1‑149 (MQ=255)
                                                                                                            tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTcc                                          <  1:1355276/149‑1 (MQ=255)
                                                                                                            tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTcc                                          >  2:87333/1‑149 (MQ=255)
                                                                                                             ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCt                                         <  1:492406/149‑1 (MQ=255)
                                                                                                                tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGAc                                      <  2:537379/149‑1 (MQ=255)
                                                                                                                     ccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTc                                 <  1:1114506/149‑1 (MQ=255)
                                                                                                                      cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                >  2:460430/1‑149 (MQ=255)
                                                                                                                      cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                <  2:1093304/149‑1 (MQ=255)
                                                                                                                       gttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                               <  1:1129691/149‑1 (MQ=255)
                                                                                                                                   aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGc                   <  1:502659/149‑1 (MQ=255)
                                                                                                                                   aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGAATCGCCTGGGCTATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCATGACCTTTACAGGCTACTACAGc                   >  2:1049260/1‑149 (MQ=255)
                                                                                                                                    cATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCa                  <  1:1374655/149‑1 (MQ=255)
                                                                                                                                     aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCTGTTATATATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCAt                 >  2:364821/1‑149 (MQ=255)
                                                                                                                                      tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATa                <  1:90983/149‑1 (MQ=255)
                                                                                                                                       aaCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg               <  1:1559308/149‑1 (MQ=255)
                                                                                                                                          gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg            <  2:62430/149‑1 (MQ=255)
                                                                                                                                          gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGATTAGTTg            <  1:10790/149‑1 (MQ=255)
                                                                                                                                            cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTc          <  1:262334/149‑1 (MQ=255)
                                                                                                                                              tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAACAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGt        <  2:802700/149‑1 (MQ=255)
                                                                                                                                               gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATTAATGCCGCCGTTATAGATAACGTACCATGTAGATGACCCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGtt       >  1:1409844/1‑149 (MQ=255)
                                                                                                                                                  ttACTGGCATCGGTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGGCGTTTGa    <  1:103610/149‑1 (MQ=255)
                                                                                                                                                   tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCTATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAc   <  1:1007066/149‑1 (MQ=255)
                                                                                                                                                   tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAc   <  1:52546/149‑1 (MQ=255)
                                                                                                                                                    aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACt  <  2:20704/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACT  >  NC_000913/3468893‑3469189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: