Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,469,050 G→A 5.8% N268N (AAC→AAT chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,469,0500GA 5.8% 261.9 / 2.7 101N268N (AAC→AATchiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base G (48/46);  new base A (3/3);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGC  >  NC_000913/3468904‑3469196
                                                                                                                                                  |                                                                                                                                                  
gTCACCGGTGTTTCCGGAGTTGGGTCACCCGGAGGCACAGCACCGCCATTTTTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATTCTGGCTGAGTTCTGTTTCCTTTGCCGAGCGACCATAACGGCAGGGGGTACTGGCCTCGtt                                                                                                                                                  >  2:1473955/1‑149 (MQ=255)
gTCACCGGTGTTTCCGGAGTTGGCTCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCGAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                                  <  1:131076/149‑1 (MQ=255)
gTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGTCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                                  >  2:1103576/1‑149 (MQ=255)
gTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtt                                                                                                                                                  >  1:1470870/1‑149 (MQ=255)
 tCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttt                                                                                                                                                 <  2:779544/149‑1 (MQ=255)
  cACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttt                                                                                                                                                <  1:1295847/149‑1 (MQ=255)
    ccGCTGATTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCACTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct                                                                                                                                              <  2:1557484/149‑1 (MQ=255)
     cGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt                                                                                                                                             >  2:52546/1‑149 (MQ=255)
     cGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTAACTGGCATCGTtttctt                                                                                                                                             >  2:90983/1‑149 (MQ=255)
     cGGTGGTTCCGGAGTTGGATCAACCGGAGGCACAGCACCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt                                                                                                                                             <  1:542817/149‑1 (MQ=255)
      ggTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttcttt                                                                                                                                            <  1:1320362/149‑1 (MQ=255)
        tgtTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCGAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg                                                                                                                                          <  1:799538/149‑1 (MQ=255)
            tCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc                                                                                                                                      >  2:1007066/1‑149 (MQ=255)
             caggAGTAGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGCACATAACTCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg                                                                                                                                     >  2:1286271/3‑149 (MQ=255)
              cGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCACCGTTTTCTTTCGCATCGc                                                                                                                                    >  2:1559308/1‑149 (MQ=255)
               ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                                   <  2:1553350/149‑1 (MQ=255)
               ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                                   >  2:381106/1‑149 (MQ=255)
                  gTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg                                                                                                                                <  2:1230757/149‑1 (MQ=255)
                   ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGCGCCAGGGTTACCATACTGACTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGTTTACTGGCATCGTTTTCTTTAGAATCGACTagg                                                                                                                               >  2:1447068/1‑149 (MQ=255)
                   ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGACAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGCACATAACGCCAAGGGTTACTGGCATCGTTTTCTTTCGCAACGCCTggg                                                                                                                               >  1:20704/1‑149 (MQ=255)
                     ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa                                                                                                                             >  2:10790/1‑149 (MQ=255)
                     ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAAAGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTGTCTTTCGCATCCCAAGGGCa                                                                                                                             >  2:10761/1‑149 (MQ=39)
                      gATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGGTTTCTTTCGCATCGCCAGGCCaa                                                                                                                            >  2:502659/1‑149 (MQ=255)
                           aCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGc                                                                                                                       >  2:1355276/1‑149 (MQ=255)
                               gAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGATGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGATTTATTTCGCATCGCCTGGGCAATTTGCcgcg                                                                                                                   >  1:1250181/1‑149 (MQ=255)
                                  tCACAGCCCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCACGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATTGGTTACAGGCATCGTTATCTTTCGCATCGCCTGGGCAATTTGACGCGCCt                                                                                                                >  2:103610/1‑149 (MQ=255)
                                  tCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGACCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGACTGGGCATTTTGCCGCCCCt                                                                                                                >  2:1076431/1‑149 (MQ=255)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGttagaaga                                                                                                                    <  2:687059/143‑7 (MQ=18)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGttagaaga                                                                                                                    >  1:687059/1‑137 (MQ=18)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                              >  2:495853/1‑149 (MQ=255)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                              >  2:262334/1‑149 (MQ=255)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                              >  2:470537/1‑149 (MQ=255)
                                    acaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                              >  2:1422814/1‑149 (MQ=255)
                                       gCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcccc                                                                                                           >  2:560374/1‑148 (MQ=255)
                                       gCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                           >  2:1296819/1‑149 (MQ=255)
                                        cACCTCCATTATTCTCTGGCTTAACGGAACATTAGCAAGTTTTACCTTAATGGCTGCTTTCTGTTGGCGTTTCAGCGTGAACTTAACGCCATGGGTTACTGGCATCTTTTTCTTTCGCATAGCATGTTCAATTTGCCGCGCCTACccac                                                                                                          <  2:321064/149‑1 (MQ=255)
                                            gCCATTAATATCCGGCTTAACGGAACAGGAGCAAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTCCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTGTGTTTCGCATCGCCAGGGAAATTTGCCGCGCCTACCCACCAgg                                                                                                      >  2:1224114/1‑149 (MQ=255)
                                              cATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCTTCGCCTGGGCAAGTTGCCGCGCCTACCCACCAGGCg                                                                                                    >  1:1259948/1‑149 (MQ=255)
                                              cATTATTATCCGGATTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg                                                                                                    <  2:708930/149‑1 (MQ=255)
                                                ttattaTCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt                                                                                                  <  2:1139082/149‑1 (MQ=255)
                                                  attatCCGGCTTCACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt                                                                                                <  2:677395/149‑1 (MQ=255)
                                                   ttatCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCGGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt                                                                                               <  2:325869/149‑1 (MQ=255)
                                                      tCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCAGGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGACGACCCCCCAGGCGTTCTtata                                                                                            >  2:249656/1‑149 (MQ=255)
                                                      acccccgtAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATATCTGTTGCCGTTGCCTCGCGAACATAACCCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCCGGCGTTCTtata                                                                                            <  2:170015/142‑1 (MQ=255)
                                                        cGGCTTAACGGAACAGGAGCCAGGGTTTCCATACTGGCTGATTTCTGTTGCCGTTGCAGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa                                                                                          <  2:183802/149‑1 (MQ=255)
                                                         ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTatatat                                                                                         >  2:383906/1‑149 (MQ=255)
                                                         ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt                                                                                         >  1:1093104/1‑149 (MQ=255)
                                                           cTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgc                                                                                       <  1:1291345/149‑1 (MQ=255)
                                                              aaCGGAACAGGAGCCAGGGTAACCATACTGGATGATTTCTGTTCCCGTTGCCGCGCGAACATAACGCCATGGGTTACAGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTAACCACCAGGCGTTCTTATAAATgccgc                                                                                    >  2:268865/1‑149 (MQ=38)
                                                               aCGGAACAGGAGACAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc                                                                                   <  1:756669/149‑1 (MQ=255)
                                                                      aGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCGTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg                                                                            <  2:560768/149‑1 (MQ=255)
                                                                        gAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCCTCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGaa                                                                          >  1:335753/1‑148 (MQ=255)
                                                                          gCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATcac                                                                                                                                    <  1:920435/89‑3 (MQ=14)
                                                                             aGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAttgc                                                                                                                                                >  1:88316/1‑72 (MQ=21)
                                                                             aGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAttgc                                                                                                                                                <  2:88316/74‑3 (MQ=21)
                                                                              gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGTGTTTCTGGCATAGTTTTCTTTCGCATCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGGTATAGATAACGTa                                                                    >  2:1011299/1‑149 (MQ=255)
                                                                               ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCAGCGCGAACATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCTGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                                   <  2:717997/149‑1 (MQ=255)
                                                                               ggTGACCATTCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGGTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                                   <  2:1181223/149‑1 (MQ=255)
                                                                                   aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAtt                                                               >  2:1278188/1‑148 (MQ=255)
                                                                                   aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGGGCTTATAATTGCCGCCGTTCTAGATAACGTACCATg                                                               >  1:871392/1‑149 (MQ=255)
                                                                                    ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATTACGTACCATGt                                                              >  1:865622/1‑149 (MQ=255)
                                                                                    ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt                                                              <  1:16099/149‑1 (MQ=255)
                                                                                     cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                             <  1:176829/149‑1 (MQ=255)
                                                                                     cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGCTACTGGCATCGATTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                             <  1:1473955/149‑1 (MQ=255)
                                                                                           ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa                                                       <  1:1285611/149‑1 (MQ=255)
                                                                                            gCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAc                                                      <  1:1217424/149‑1 (MQ=255)
                                                                                             cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGGAGAAGAAcc                                                     >  2:199757/1‑149 (MQ=255)
                                                                                              tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                                    >  1:740448/1‑149 (MQ=255)
                                                                                              tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAGGTAGAAGAACCt                                                    >  1:436974/1‑149 (MQ=255)
                                                                                                aTTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTc                                                  <  1:381106/149‑1 (MQ=255)
                                                                                                 tttCTTTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTCGCAACGTTTTCTTACGCATCGCCTCGGCAATTTTCCGCGCCTACACACCACGCGTACTTATAAATGCCGCCGTAATAGATAACGTACAATATAGAAGAACCTTcc                                                 >  2:407752/1‑149 (MQ=255)
                                                                                                 tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTcc                                                 >  2:87333/1‑149 (MQ=255)
                                                                                                 tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTcc                                                 <  1:1355276/149‑1 (MQ=255)
                                                                                                  ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCt                                                <  1:492406/149‑1 (MQ=255)
                                                                                                     tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGAc                                             <  2:537379/149‑1 (MQ=255)
                                                                                                          ccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTc                                        <  1:1114506/149‑1 (MQ=255)
                                                                                                           cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                       >  2:460430/1‑149 (MQ=255)
                                                                                                           cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                       <  2:1093304/149‑1 (MQ=255)
                                                                                                            gttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                                      <  1:1129691/149‑1 (MQ=255)
                                                                                                                        aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGc                          <  1:502659/149‑1 (MQ=255)
                                                                                                                        aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGAATCGCCTGGGCTATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCATGACCTTTACAGGCTACTACAGc                          >  2:1049260/1‑149 (MQ=255)
                                                                                                                         cATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCa                         <  1:1374655/149‑1 (MQ=255)
                                                                                                                          aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCTGTTATATATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCAt                        >  2:364821/1‑149 (MQ=255)
                                                                                                                           tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATa                       <  1:90983/149‑1 (MQ=255)
                                                                                                                            aaCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg                      <  1:1559308/149‑1 (MQ=255)
                                                                                                                               gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg                   <  2:62430/149‑1 (MQ=255)
                                                                                                                               gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGATTAGTTg                   <  1:10790/149‑1 (MQ=255)
                                                                                                                                 cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTc                 <  1:262334/149‑1 (MQ=255)
                                                                                                                                   tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAACAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGt               <  2:802700/149‑1 (MQ=255)
                                                                                                                                    gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATTAATGCCGCCGTTATAGATAACGTACCATGTAGATGACCCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGtt              >  1:1409844/1‑149 (MQ=255)
                                                                                                                                       ttACTGGCATCGGTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGGCGTTTGa           <  1:103610/149‑1 (MQ=255)
                                                                                                                                        tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCTATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAc          <  1:1007066/149‑1 (MQ=255)
                                                                                                                                        tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAc          <  1:52546/149‑1 (MQ=255)
                                                                                                                                         aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACt         <  2:20704/149‑1 (MQ=255)
                                                                                                                                            ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTAt      <  2:1470870/149‑1 (MQ=255)
                                                                                                                                             gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAGTTATc     >  2:176661/1‑149 (MQ=255)
                                                                                                                                             gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATc     <  1:470537/149‑1 (MQ=255)
                                                                                                                                             gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATc     >  1:809168/1‑149 (MQ=255)
                                                                                                                                               aTCGTTTTCTTTCGCATCGCCTGGGCAAATTGCCGCGCCTACCCACAAGGCTTTCATATAAATGCCGCAGTTATAGAAAACGTACCATGTTGAATAACCTTAATGACCGTTCAGGGCTCAGACCGCAGAGTTGTCGTTTGACGTATctg   >  2:212230/1‑147 (MQ=255)
                                                                                                                                                tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGc  >  1:1254344/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGC  >  NC_000913/3468904‑3469196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: