Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,573 G→A 5.5% intergenic (‑30/+47) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5730GA 5.5% 251.7 / 3.1 93intergenic (‑30/+47)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (48/38);  new base A (2/3);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

CCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCG  >  NC_000913/265426‑265719
                                                                                                                                                   |                                                                                                                                                  
ccaGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGc                                                                                                                                                   >  1:1506924/1‑149 (MQ=255)
     cGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCt                                                                                                                                              >  1:829861/1‑149 (MQ=255)
       gAAGTGACTGCCCTGGGCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGc                                                                                                                                            <  1:1228114/149‑1 (MQ=255)
        acgTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCt                                                                                                                                           <  2:766330/147‑1 (MQ=255)
               tGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTc                                                                                                                                    >  2:2899/1‑149 (MQ=255)
                 cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGCGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCAAGCTGTCgg                                                                                                                                  >  2:692453/1‑149 (MQ=255)
                  ccTGGTCGTCTTCGAGTGTGATCTGGCTGTACGTGGTCGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                 <  2:56767/149‑1 (MQ=255)
                  ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCATCACGGATTGGGTCATTTTCGGGCGCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                 <  1:713651/149‑1 (MQ=255)
                  ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                 <  2:1187565/149‑1 (MQ=255)
                  ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                 <  1:401421/149‑1 (MQ=255)
                  ccTGGTCGTCTTCGAGGGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                 <  1:1543464/149‑1 (MQ=255)
                   cTGGTCGGCTTCGAGTGTGAAATTGCTGGACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAACGGCCCCGGTGGCAGCAACACGCATTGCGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTGGCTGTCgggg                                                                                                                                <  2:743377/149‑1 (MQ=255)
                      gtcgtcTTCGAGTGTGATACTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGa                                                                                                                             >  1:1440896/1‑149 (MQ=255)
                       gcgtcTTCGAGTGTGATATTGCTGTACGTTGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCCTTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGaa                                                                                                                            <  2:1117662/148‑1 (MQ=255)
                        cgtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAt                                                                                                                           <  2:498488/149‑1 (MQ=255)
                            ttCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                       >  2:942558/1‑149 (MQ=255)
                              cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGTATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                     <  1:1394416/149‑1 (MQ=255)
                              cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                     <  2:1536536/149‑1 (MQ=255)
                                   gtgATATTGCTCTACGTGGTAGTGTCCGCTTGCGCTTGTCTCCGGGGGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGCCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGaa                                                                                                                <  1:1082177/149‑1 (MQ=255)
                                    tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAg                                                                                                               >  1:943905/1‑149 (MQ=255)
                                        atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtac                                                                                                           >  1:260371/1‑149 (MQ=255)
                                          tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                         <  1:962664/149‑1 (MQ=255)
                                          tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCGCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAACCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                         <  1:501974/149‑1 (MQ=255)
                                            cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                       >  1:460909/1‑149 (MQ=255)
                                            cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                       <  2:298711/149‑1 (MQ=255)
                                               gtcGTGTTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATc                                                                                                    <  1:291984/147‑1 (MQ=255)
                                                         tGACCGCTTGAGCTTGGCTCCGGGTGAAAGGCCCCTTTGGCAGCAACACGGTTTGGGTCATTTTCGGGCTCCTGATAAAACAAAACCCAGGCAGCCTGCTATCTGTCGTGGTGAATTTGCTGGGGAAGATACTACTATCAGGCGTtgct                                                                                          <  1:178790/149‑1 (MQ=255)
                                                           aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                        >  1:591501/1‑149 (MQ=255)
                                                           aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                        >  2:1183322/1‑149 (MQ=255)
                                                            ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGTTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                       <  2:182810/149‑1 (MQ=255)
                                                            ccGCTTGCGCTTGGCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                       <  2:414699/149‑1 (MQ=255)
                                                              gcttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCACCACGGAGTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGt                                                                                     >  1:1004790/1‑149 (MQ=255)
                                                               cttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtc                                                                                    >  1:813151/1‑149 (MQ=255)
                                                                 tgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCACCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtctc                                                                                  <  2:669211/149‑1 (MQ=255)
                                                                  gcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAGAGAAAACCCCGGCAGCCGGCGAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTcc                                                                                 >  1:708628/1‑149 (MQ=255)
                                                                  gcgcttgTCGCCGGGTGACAGGCCCCGGTGGCAGCAACACGGCTTGGGGCATTTTCGGGCTCCTGATAAAAGCAAACCCCGGCAGCCAGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGGTGCTGCAGTCTcc                                                                                 <  2:1396638/149‑1 (MQ=255)
                                                                    gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCga                                                                               >  2:1561238/1‑149 (MQ=255)
                                                                         tctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                          >  1:620399/1‑149 (MQ=255)
                                                                         gctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                          <  1:442185/148‑1 (MQ=255)
                                                                           tcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                        >  1:231888/1‑149 (MQ=255)
                                                                             cGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACa                                                                      <  2:829861/149‑1 (MQ=255)
                                                                              gggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAg                                                                     >  2:773528/1‑149 (MQ=255)
                                                                                gggAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                   <  1:380499/147‑1 (MQ=255)
                                                                                gTGAAAGGCCCCGGTGGCAGCAACACGGATGGGGTCATTTTCGGGCGCCTGATAACAGAAACCACCGGCCGCCAGCTCGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                   <  1:856161/149‑1 (MQ=255)
                                                                                 tGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaaa                                                                  >  2:1496520/1‑148 (MQ=255)
                                                                                 ggAAAGGCCCCGGTGGAAGCATCACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAAc                                                                  <  1:415261/148‑1 (MQ=255)
                                                                                  gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACt                                                                 >  2:740859/1‑149 (MQ=255)
                                                                                  gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGCCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTTCTGCAGTCTCCGAGAGTGGACAGAACt                                                                 >  2:490013/1‑149 (MQ=255)
                                                                                   aaaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACtt                                                                >  2:1360474/1‑149 (MQ=255)
                                                                                        ccccGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCATCCTGCTAGCTTTCGGGGTGAATATGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTGTCTCa                                                           >  2:392614/1‑149 (MQ=255)
                                                                                             gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTACCTGTCGGCGTGAATTTGCTGGGGAAGCTACTACTATCAGTCGTTGCTGCAGTCTCCGAGCGTGGACAGCACTTTCTCAGCGtt                                                      >  1:1051286/1‑149 (MQ=255)
                                                                                                   gctccccGGATTGGGGCATTTTCGGGCGCCTGATAAACGCAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCg                                                <  2:60728/143‑1 (MQ=255)
                                                                                                      acacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTc                                             <  2:1506924/149‑1 (MQ=255)
                                                                                                       cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGCCAGACCTTTCTCAGCGTTCTTCCGGTcc                                            >  2:848036/1‑149 (MQ=255)
                                                                                                       cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTcc                                            <  2:599465/149‑1 (MQ=255)
                                                                                                          ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTTTCGGGGTCAATATGCTGGGGAAGATACAACTATCAATCGTTTCTGCAGTCTCCGACAGTGGACAGAACATTCTCAGCGTTCTTCCgcacagcc                                         >  2:1452361/1‑142 (MQ=255)
                                                                                                          ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                         >  1:314936/1‑149 (MQ=255)
                                                                                                          ggATTGGGCCATTTTCGGGCGCCTGATAAAATAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCCTTGCTGCAGTCTCCGAGATTGGACTGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                         <  2:1174771/149‑1 (MQ=255)
                                                                                                             ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGATGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                      >  1:963019/1‑149 (MQ=255)
                                                                                                             ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                      <  1:223745/149‑1 (MQ=255)
                                                                                                               gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCTCAGTGaa                                    <  2:616099/149‑1 (MQ=255)
                                                                                                               gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCACAGCGTTCTTCCGGTCCGCAGTgca                                    >  2:1349865/1‑147 (MQ=255)
                                                                                                                gggcATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                   <  2:1175254/146‑1 (MQ=255)
                                                                                                                ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                   >  1:1480350/1‑149 (MQ=255)
                                                                                                                ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                   >  2:476549/1‑149 (MQ=255)
                                                                                                                ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                   >  2:836620/1‑149 (MQ=255)
                                                                                                                ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                   >  2:774560/1‑149 (MQ=255)
                                                                                                                    cttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcc                               <  2:957383/148‑1 (MQ=255)
                                                                                                                     ttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAACAGTCGTTGCTGCAGTCGCCGAGAGTGGCCAGACGTTTGTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                              >  1:488286/1‑149 (MQ=255)
                                                                                                                     ttttCGGGCTCCTGATAAAAGAAAACCACGGCAGCCTGCTAGCTGTCGGGGGGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGTGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                              >  1:868041/1‑149 (MQ=255)
                                                                                                                      tttCGGGCTCCTGATAAAAGAAAACCCCGTCCGCCTGCTAGCTGTCGGGGTGAATTTTCTGGGGAAGATACGACTTTCAGTCGTTGCTGCAGTCTCTGAGAGTGGGCAGCACGTTCTCAGCGTTCGTCCGGTCCGCAGTGAAGGTCCCt                             >  2:1237896/1‑149 (MQ=255)
                                                                                                                        tCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGc                           <  1:1426041/149‑1 (MQ=255)
                                                                                                                            gCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtg                       >  2:1062958/1‑149 (MQ=255)
                                                                                                                             cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                      <  2:604664/149‑1 (MQ=255)
                                                                                                                             cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                      >  1:21918/1‑149 (MQ=255)
                                                                                                                              tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                     <  2:260371/149‑1 (MQ=255)
                                                                                                                               ccTGAGAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                    >  1:327430/1‑149 (MQ=255)
                                                                                                                                cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtgtg                   >  2:1404951/1‑147 (MQ=255)
                                                                                                                                cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTAATATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCGCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                   >  2:846774/1‑149 (MQ=255)
                                                                                                                                 tGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAGCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTc                  <  2:267818/149‑1 (MQ=255)
                                                                                                                                  gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCa                 <  1:2899/149‑1 (MQ=255)
                                                                                                                                    aaaaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAtt               <  2:620989/148‑1 (MQ=255)
                                                                                                                                       aaGAAAACCCTGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGATCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc            >  1:483090/1‑149 (MQ=255)
                                                                                                                                       aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc            >  1:1198436/1‑149 (MQ=255)
                                                                                                                                        cgAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTCTCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGTACTTTCTCAGCGTTCTTCCGGGCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACg           <  2:952455/148‑1 (MQ=255)
                                                                                                                                        aGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGACTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg           >  2:912286/1‑149 (MQ=255)
                                                                                                                                         gAAAACCCCGGCAGCCTGCTTGCTGTCGGGGTGAATTTGCTGGGGAAGATTCTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt          >  1:1422935/1‑149 (MQ=255)
                                                                                                                                            aaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGACCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACTTAGa       >  1:698981/1‑149 (MQ=255)
                                                                                                                                             aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGCACTTTCACAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc      >  1:1014540/1‑149 (MQ=255)
                                                                                                                                             aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc      >  2:1471458/1‑149 (MQ=255)
                                                                                                                                              ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTACCGGTCCGCAGTGAAGGTCCCTGATTAGTGGTCATTGACGTAGCCg     >  2:1032556/1‑149 (MQ=255)
                                                                                                                                               cccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt    >  1:923105/1‑149 (MQ=255)
                                                                                                                                                 cGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg  >  2:978915/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCG  >  NC_000913/265426‑265719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑