Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,642,358 T→A 5.5% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,642,3580TA 5.5% 278.7 / 2.5 100intergenic (+27/+22)dtpB/rsmJdipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base A (3/3);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CGTTGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCC  >  NC_000913/3642210‑3642503
                                                                                                                                                    |                                                                                                                                                 
cGTTGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGt                                                                                                                                                   <  1:448344/149‑1 (MQ=255)
  ttGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTTTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTa                                                                                                                                                 >  1:96204/1‑149 (MQ=255)
      ccGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGCCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGc                                                                                                                                             >  1:205396/1‑149 (MQ=255)
      ccGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATcca                                                                                                                                             >  1:778404/1‑147 (MQ=255)
      ccGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGc                                                                                                                                             >  1:1281187/1‑149 (MQ=255)
           tataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTAccc                                                                                                                                        <  2:773658/149‑1 (MQ=255)
            ataCCAACGTGTTTGGTAAGATTTGTCTGGTCAGGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt                                                                                                                                       <  1:1359045/149‑1 (MQ=255)
              aCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGAGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgt                                                                                                                                     <  2:1022775/149‑1 (MQ=255)
               ccAACGTGTTTGTTAAGTTTGGTCTGGTCACGCTGGGCGTTGCTGTAGTGATGCTGTTGATGGTGCCGGGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCTgct                                                                                                                                    <  2:407391/149‑3 (MQ=255)
               ccAACGTGTTTGGTAAGATTGGTCTGGGCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGCGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtt                                                                                                                                    <  1:1357969/149‑1 (MQ=255)
               ccAACGTGGTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtt                                                                                                                                    <  1:971504/149‑1 (MQ=255)
                ctaCGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttt                                                                                                                                   <  1:269230/147‑1 (MQ=255)
                    gtgtTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagt                                                                                                                               <  1:808113/149‑1 (MQ=255)
                      gtTTGGTAAGATTGGTCTGGTCACGCGGGGCGTTGCAGTAGTGCTGCTGTTGATGGTGCCGTGGCGGAAACGCATGATTTCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagttt                                                                                                                             <  2:799387/149‑1 (MQ=255)
                       tttGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttt                                                                                                                            >  1:1191319/1‑149 (MQ=255)
                        gtGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGACACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtttta                                                                                                                           <  1:88276/148‑1 (MQ=255)
                          ggTAAGATTGGGCTGGTCCCGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGAGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTACTTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTaca                                                                                                                         <  1:344130/149‑1 (MQ=255)
                           gTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTacac                                                                                                                        <  1:602379/149‑1 (MQ=255)
                            tAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGCACCGCATGATTGCGACGCCGGAAAGCCAGTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACt                                                                                                                       >  1:1086450/1‑149 (MQ=255)
                            tAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACt                                                                                                                       >  1:485028/1‑149 (MQ=255)
                             aaGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTg                                                                                                                      >  1:433157/1‑149 (MQ=255)
                               gATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTAGCCTGTTTTAGTTTTACACTGGc                                                                                                                    >  1:504843/1‑149 (MQ=255)
                               gATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGc                                                                                                                    <  2:284864/149‑1 (MQ=255)
                               gATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGAGGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAAATATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGc                                                                                                                    <  1:1189286/149‑1 (MQ=255)
                                  tggtctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTa                                                                                                                 >  2:950714/1‑149 (MQ=255)
                                   ggtctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTAc                                                                                                                <  2:876427/149‑1 (MQ=255)
                                       tggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGc                                                                                                            <  1:1431039/149‑1 (MQ=255)
                                       tggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGc                                                                                                            >  1:309950/1‑149 (MQ=255)
                                          tcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATa                                                                                                         >  1:920297/1‑149 (MQ=255)
                                            aCGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGa                                                                                                       <  1:289017/149‑1 (MQ=255)
                                              gCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGata                                                                                                     <  2:978226/149‑1 (MQ=255)
                                              gCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGata                                                                                                     >  2:1284135/1‑149 (MQ=255)
                                               cTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCAGTAATTATTCTTGCAGACAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACAATGGCGTCCCTGCAAAGataa                                                                                                    >  1:1244024/1‑148 (MQ=255)
                                                 gggCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCa                                                                                                  <  2:551319/149‑1 (MQ=255)
                                                   gCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCaaa                                                                                                <  1:377165/149‑1 (MQ=255)
                                                     gTTGCAGTTGTGATGCTGTTGATGGTGCCGTTGCTTAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTTCAGAAAGCAGGGTAGCGTTATCGCTACGCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGc                                                                                              <  1:711068/149‑1 (MQ=255)
                                                        gCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTATACTGGCGTACCTGCATAGATATCAAAGCGAt                                                                                           <  2:1195073/149‑1 (MQ=255)
                                                         cAGTAGTGATGGTGTTGATGGGGCCGTGGCTGAAACGCATGATTGCGACGCCGTAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATg                                                                                          <  2:96204/149‑1 (MQ=255)
                                                            tagtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTAGCGTACCTGCATAGATTTCAAAGCGATGccc                                                                                       >  1:518665/1‑149 (MQ=255)
                                                              gtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCtt                                                                                     <  1:679194/149‑1 (MQ=255)
                                                              gtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCtt                                                                                     >  1:707634/1‑149 (MQ=255)
                                                                gATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGAAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTa                                                                                   >  1:524802/1‑149 (MQ=255)
                                                                gATGCTGTTGATGGTGCAGTGGCGGCAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTa                                                                                   <  2:1553509/149‑1 (MQ=255)
                                                                 aTGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGat                                                                                                      <  2:1162857/129‑1 (MQ=255)
                                                                 aTGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGat                                                                                                      >  1:1162857/1‑129 (MQ=255)
                                                                 aTGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAg                                                                                  >  2:1331616/1‑149 (MQ=255)
                                                                  tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt                                                                                 <  1:280719/149‑1 (MQ=255)
                                                                  tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt                                                                                 >  1:1519236/1‑149 (MQ=255)
                                                                  ggCTGTTGATGGTGCCGTGGCTGAAACTCATGATTGCGACGCCGGAAAGCCATAAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATAGCAAAGCGATGCCCTTTAGt                                                                                 <  2:1065974/148‑1 (MQ=255)
                                                                     tGTTGATGGTGCGGTGGCTGTAACGGATGATTGCGACTGCGGTAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATATATATCAAAGCGATGCCCTTTAGTGAc                                                                              <  1:804626/149‑1 (MQ=255)
                                                                     tGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGAc                                                                              <  1:827001/149‑1 (MQ=255)
                                                                      gTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACt                                                                             >  2:839835/1‑149 (MQ=255)
                                                                      gTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATGCTGGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACGGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACt                                                                             >  2:707592/1‑149 (MQ=255)
                                                                          aTGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCATTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACcg                                                                         >  1:45936/1‑149 (MQ=255)
                                                                           tGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACcgc                                                                        >  1:620316/1‑149 (MQ=255)
                                                                               gCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGttt                                                                    <  2:130722/149‑1 (MQ=255)
                                                                                  gTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAACGCGATGCCCTTTAGTGACTACCGCGTTtggc                                                                 >  1:1458436/1‑149 (MQ=255)
                                                                                  gTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCGTTTAGTGCCTACCGCGTTtggc                                                                 >  2:1439569/1‑149 (MQ=255)
                                                                                    ggCTGAAACGCATGATTGCGACGCCGGAACGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgt                                                               >  1:1413290/1‑149 (MQ=255)
                                                                                     gCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtg                                                              <  1:563578/149‑1 (MQ=255)
                                                                                      cTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtgg                                                             <  1:429306/149‑1 (MQ=255)
                                                                                      cTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACAGCGTTtggcgtgg                                                             <  2:1204860/149‑1 (MQ=255)
                                                                                       tGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATTCCCTTTAGTGACTACCGCGTTtggcgtggc                                                            >  1:632267/1‑149 (MQ=255)
                                                                                       tGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtggc                                                            >  1:1231241/1‑149 (MQ=255)
                                                                                         aaaCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGa                                                          >  2:752540/1‑149 (MQ=255)
                                                                                             gCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGGTTGGCGTGGCGACAtt                                                      >  1:1364421/1‑149 (MQ=255)
                                                                                              cATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTc                                                     <  2:551314/149‑1 (MQ=255)
                                                                                                tGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATAAAAGCGATGCCCTATAGTGACTACCGCGTTTGGCGTGGCGACATTCGc                                                   >  2:670756/1‑149 (MQ=255)
                                                                                                  aTTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATCCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCa                                                 <  1:952570/149‑1 (MQ=255)
                                                                                                    tGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGc                                               >  1:78494/1‑149 (MQ=255)
                                                                                                     gCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCg                                              <  2:1405076/149‑1 (MQ=255)
                                                                                                     gCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCg                                              <  2:524802/149‑1 (MQ=255)
                                                                                                     gCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTGTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGCATACCGCGTTTGGCGTGGCGACTTTCGCCcgcg                                              >  2:1529015/1‑149 (MQ=255)
                                                                                                       gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGt                                            >  2:1212399/1‑149 (MQ=255)
                                                                                                       gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGt                                            <  1:980730/149‑1 (MQ=255)
                                                                                                        aCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTg                                           <  2:1191319/149‑1 (MQ=255)
                                                                                                        aCGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTg                                           >  2:120305/1‑149 (MQ=255)
                                                                                                          gCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGAGGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgc                                         <  1:371998/149‑1 (MQ=255)
                                                                                                            cGGAAAGCCATTAATTATTCTTGCAGAACGCAGGGTAGAGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCAAAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGGGgcgc                                       <  2:1452392/149‑1 (MQ=255)
                                                                                                            cGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgcgc                                       >  1:668159/1‑149 (MQ=255)
                                                                                                                    cATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGAGAACCTGCATACTTATCAAAGCGATGCCATTTAGTGACTACAGCGTTTGGCGTGGCTACATTCGCCAGCGGTGGCGCGTAGTCCg                               >  2:568119/1‑149 (MQ=255)
                                                                                                                    cATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATAGCTACCCTGTTTTAGTTTTACACTGGCTTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCg                               >  1:269725/1‑149 (MQ=255)
                                                                                                                     attaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACAATGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGTCGCGTAGTCCgg                              >  1:988209/1‑149 (MQ=255)
                                                                                                                     attaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGATACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACGTTCGCCCGCGGTGGCGCGTAGTCCgg                              >  1:1232024/1‑149 (MQ=255)
                                                                                                                      ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCggg                             >  1:1240237/1‑149 (MQ=255)
                                                                                                                              tCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTac                     <  1:752540/149‑1 (MQ=255)
                                                                                                                                  gCAGAAAGCAGGGTAGGGTTATCGCTACGCTGTTTTAGGTTTACACTGGCGTACCTGCAGAGCTATCAAAGCGCTGCCCTTTAGTGCGTACCGCGTTTGGCGTGGCGACATTCGCCCGCGGTGGCGCGTAGTCCGGGGGCtttcgctcc                 >  1:1371570/1‑143 (MQ=255)
                                                                                                                                  gCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTaccacc                 >  1:74255/1‑149 (MQ=255)
                                                                                                                                     taaaaCAGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCCCCAcg              >  1:133874/2‑149 (MQ=255)
                                                                                                                                     taaaaCAGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCAcg              >  2:24102/2‑149 (MQ=255)
                                                                                                                                     gAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATTTCAAAGCGATGCCCTTTAGTGACTACCTCGTTTGGCGTGGCGACATTCGCCCGCGGTGGCGGGTAGTCCGGGCGCTTTACGACCAcg              >  1:1038407/1‑149 (MQ=255)
                                                                                                                                      acaGCAGGGTAGCGTTTTCGCTACCCGGTTATAGTTTTCGAGTGGCGTACCTGCATAGATATCAAAGCGATGCCCTGTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCAcgc             <  1:1061696/147‑1 (MQ=255)
                                                                                                                                       aaacAGGGTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCAcgcg            >  2:878803/4‑149 (MQ=255)
                                                                                                                                        aGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGt           >  2:703959/1‑149 (MQ=255)
                                                                                                                                         gCAGGGTAGCGTTATCGCTACCCTGGTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGtt          <  2:309950/149‑1 (MQ=255)
                                                                                                                                          cAGGGTAGCGATATGTCTACCCTGTTTTAGTTGTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTGTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt         <  2:455558/149‑1 (MQ=255)
                                                                                                                                           aGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTAACTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTg        <  1:950714/149‑1 (MQ=255)
                                                                                                                                              gTAGCGTTATCGCTACCCTGTTTTAGTTTTAGACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCTCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTc     <  1:239095/149‑1 (MQ=255)
                                                                                                                                                aGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCAGCACGCGTTTGGTCGc   <  1:814750/149‑1 (MQ=255)
                                                                                                                                                 gCGTTATCGCTACCATGTTTTAGTTTTACACTTGCGTTCCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGcc  <  2:778404/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGTTGCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCC  >  NC_000913/3642210‑3642503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: