Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,798,235 A→T 5.1% intergenic (+28/+4) waaL → / ← waaU O‑antigen ligase/lipopolysaccharide core biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,798,2350AT 5.1% 422.2 / 6.1 143intergenic (+28/+4)waaL/waaUO‑antigen ligase/lipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (63/72);  new base T (5/3);  total (68/75)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCGGA  >  NC_000913/3798088‑3798383
                                                                                                                                                   |                                                                                                                                                    
ggCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTaaa                                                                                                                                                     <  2:137855/149‑1 (MQ=255)
ggCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTaaa                                                                                                                                                     >  1:1404422/1‑149 (MQ=255)
 gCATTGTGGGGATTGGACTCAGTGATGTGATCATTTGGGCACGCAGCATTGCAATTATCATTATATCCGGTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTaaaa                                                                                                                                                    <  2:1285833/149‑1 (MQ=255)
 gCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTAAATCCGCAATAGTCCTCTAACTCGTCATTAATAATCGTAACAATACAATTAATTAATAATAAACAAGTTTAAGAAGTGAGTTaaaa                                                                                                                                                    >  2:1271376/1‑149 (MQ=255)
     tgtgGGGATTGGACTCAGTTATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCa                                                                                                                                                <  1:966441/149‑1 (MQ=255)
       tgGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTAACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCAct                                                                                                                                              >  2:356624/1‑149 (MQ=255)
        ggggATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCActt                                                                                                                                             <  2:642850/149‑1 (MQ=255)
           gATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCATTTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACTAATAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCActactt                                                                                                                                          >  2:392736/1‑149 (MQ=255)
             ttGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCTCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTAt                                                                                                                                        <  1:374986/149‑1 (MQ=255)
             ttGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTAt                                                                                                                                        <  1:366236/149‑1 (MQ=255)
             ttGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACGAACTTCTTAt                                                                                                                                        >  1:888390/1‑149 (MQ=255)
              tGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTTTAACTCACTTCTTaaa                                                                                                                                       <  1:1236068/149‑3 (MQ=255)
              tGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATc                                                                                                                                       >  2:426465/1‑149 (MQ=255)
                taCTCAGTGATGTTTTCATATGGGTACGCAGCATTACAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCta                                                                                                                                     <  2:868418/148‑1 (MQ=255)
                gACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCta                                                                                                                                     >  1:336578/1‑149 (MQ=255)
                gACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCta                                                                                                                                     >  1:1232440/1‑149 (MQ=255)
                     aGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACaa                                                                                                                                >  1:1329208/1‑149 (MQ=255)
                         atgtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTa                                                                                                                            >  2:15358/1‑149 (MQ=255)
                          tgtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTaa                                                                                                                           >  1:574694/1‑149 (MQ=255)
                           gtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAt                                                                                                                          <  1:1157349/149‑1 (MQ=255)
                              atCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATCCAATTACTTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtctc                                                                                                                       <  2:1032345/149‑1 (MQ=255)
                                cATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATCCAATTAATTAAGAATACACAAGTTTAAGAAGTGAGTTAACACTCACTTCTTATCTATACAACTTAATCTCtt                                                                                                                     <  2:721269/149‑1 (MQ=255)
                                 atatGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCttt                                                                                                                    >  2:764182/1‑149 (MQ=255)
                                 atatGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCttt                                                                                                                    <  2:522487/149‑1 (MQ=255)
                                 atatGGGCACGCAGCATTCCAATTATCAATATATCCGCTATAGTCCTCTTACTCGTCATTAATATTCGTAACAAAACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTATCACTGAGTTCTTTTCTATACAACTCTATCTCttt                                                                                                                    >  1:829921/1‑149 (MQ=255)
                                  tatGGGCACGCAGCATTCAAATTATCATAATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTa                                                                                                                   <  2:654209/149‑1 (MQ=255)
                                   atGGGCACGCAGCATTACATTTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTAc                                                                                                                  <  2:1437070/149‑1 (MQ=255)
                                        cACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAAt                                                                                                             <  1:71801/149‑1 (MQ=255)
                                         aCGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAAtt                                                                                                            <  1:1077535/149‑1 (MQ=255)
                                           gcagcaTTCCAATTATCATTATATCCGCTATAGTCCTCATACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTAACTTCTTATCTATACAAATTAATCTCTTTACTGAATTat                                                                                                          >  1:1145329/1‑148 (MQ=255)
                                            cagcaTTCCAATTATCATTATATCCGCAATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGt                                                                                                         <  2:696559/149‑1 (MQ=255)
                                             agcaTTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTTATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGATTTAGtt                                                                                                        >  2:782507/1‑149 (MQ=255)
                                              gcaTTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTa                                                                                                       <  1:1255898/149‑1 (MQ=255)
                                                aTTCCAATTATCATTATATCCGCTATAGTCCTATTACTAGTAATTAATAATCGTAACAATACAATTAATTAAGAATAAACTAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTaaa                                                                                                     <  1:405822/149‑1 (MQ=255)
                                                 ttCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAg                                                                                                    >  2:912321/1‑149 (MQ=255)
                                                   ccAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGtt                                                                                                  >  1:1260754/1‑149 (MQ=255)
                                                     aaTTATCATTATATCCGCAATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTc                                                                                                <  2:236702/149‑1 (MQ=255)
                                                      tttATCATTATATCCGCTATTGTCCTGTTACTCGTCATTAATAATCGTTAGATTGCATTTAATTTAGGATAAACAAGTTTAAGAAGTGAGTTAATTCTCAGTTCTTATGTATACAAGTTAATCGCTGTACTGATTTAGTTATAGTTTCa                                                                                               >  2:161603/2‑149 (MQ=255)
                                                      aTTATCATTATATACGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAAAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCa                                                                                               <  2:1228532/149‑1 (MQ=255)
                                                        tATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGt                                                                                             <  1:38214/149‑1 (MQ=255)
                                                             ttATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGa                                                                                        >  1:665318/1‑149 (MQ=255)
                                                              tataTCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGat                                                                                       <  2:1432871/149‑1 (MQ=255)
                                                                 atCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATc                                                                                    >  1:199406/1‑149 (MQ=255)
                                                                   ccGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCtt                                                                                  >  1:1269673/1‑149 (MQ=255)
                                                                      atataGTCCTCTTAATCTTCATTAATAATCGTAAAAATACAATTAATTAATAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTa                                                                               <  1:74620/148‑1 (MQ=255)
                                                                       tataGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTTATTAAGAATAAACATTTTTATGAAGTGATTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTAc                                                                              <  2:1109279/149‑1 (MQ=255)
                                                                        ataGTCCTCTTACTCGTAATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTAcc                                                                             <  2:494372/149‑1 (MQ=255)
                                                                        ataGTCCTCATACTCGTCATTAATAATCGAAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTAcc                                                                             <  1:294694/149‑1 (MQ=255)
                                                                            tCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGtat                                                                         <  2:186681/149‑1 (MQ=255)
                                                                            tCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGtat                                                                         <  2:1555695/149‑1 (MQ=255)
                                                                            tCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTCATCTATACAACTTAATATATTTACTGAATTAGTTAAAGTTTAAGTATCGATATCTTTTACCGtat                                                                         >  2:1076289/1‑149 (MQ=255)
                                                                               tctTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTatatt                                                                      >  2:688654/1‑148 (MQ=255)
                                                                                   aCTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTgg                                                                  >  2:2486/1‑149 (MQ=255)
                                                                                    cTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATctct                                                                                                                      <  2:1302529/96‑1 (MQ=255)
                                                                                     tCGTCATTAATAATCGTAACAATACAATTAATTAAGAAAAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACATAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACAGTAAATGTTtggt                                                                >  2:475901/1‑148 (MQ=255)
                                                                                      cGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGa                                                               <  1:356624/149‑1 (MQ=255)
                                                                                       gTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGaa                                                              >  1:994951/1‑148 (MQ=255)
                                                                                       gTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGAt                                                              >  1:470747/1‑149 (MQ=255)
                                                                                            taataaTCGTAACAATACAATTAATTAATAATAAACAAGTTTAAGCAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTGTTTACTGACTTAGGTAAAGTTTCAGTCTCGATATCTTTTACCGTATCTGTTGGGGATACTAt                                                         <  1:845871/149‑1 (MQ=255)
                                                                                              ataatCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTAttt                                                       <  1:370351/149‑1 (MQ=255)
                                                                                              ataatCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTAttt                                                       >  2:877846/1‑149 (MQ=255)
                                                                                              ataatCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAAAGTAATCTCTTTACTGAATTAGTTAAAGTTTCTGTATCGATATCTTTTACAGTATATGTTTGGTATACTAttt                                                       >  2:524176/1‑149 (MQ=255)
                                                                                               taatAGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTCGTTAAAGTTTCAGTATAGATATCTTTTACCGTATATGTTGGGGATACTATTTg                                                      <  1:103339/149‑1 (MQ=255)
                                                                                                aatCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATCTGTTGGGGATACTATTTGt                                                     <  2:1366125/149‑1 (MQ=255)
                                                                                                 atCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAAATCACTTCTTATATATACAACAAAATCTCATTAATGAATTATTTAAAGATTCAGTATCGATATATATAACCGTATATGTTGGGGATACTATTTCta                                                    >  1:379934/1‑149 (MQ=255)
                                                                                                  acGTAACAAAACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACAGAATTAGATAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGtat                                                   <  2:873369/148‑1 (MQ=255)
                                                                                                      aaCAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGAt                                               >  2:1524993/1‑149 (MQ=255)
                                                                                                        caatacaatTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGAttt                                             <  2:218103/149‑1 (MQ=255)
                                                                                                        aaatagaatTAATTAAGAATAAACAAGTTTAAGTAGTGAGTTTAAACTCACTTCTTATCTATAGAACTTAATCTCTTTAGTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGtttt                                             <  1:475901/148‑1 (MQ=255)
                                                                                                          atacaatTAATTACGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCCTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTat                                           <  2:118075/149‑1 (MQ=255)
                                                                                                             caatTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTatgat                                        <  2:1248751/149‑1 (MQ=255)
                                                                                                              aattaattAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTatgatg                                       >  1:559678/1‑149 (MQ=255)
                                                                                                              aattaattAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTatgatg                                       <  1:1271376/149‑1 (MQ=255)
                                                                                                              aattaattAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACAGTATATGTTGGGGATACTATTTGTTTAGATTTatgatg                                       >  1:263692/1‑149 (MQ=255)
                                                                                                                ttaattaaGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATgatgat                                     >  1:1102826/1‑149 (MQ=255)
                                                                                                                   attaaGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACAGAATTTGTTAAAGTTTCAGTATCGATATCTTTTACCGTTTATGTTGGGGATACTATTTGTATAGATTTATGATGATTCg                                  >  1:1428350/1‑149 (MQ=255)
                                                                                                                    ttaaGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCgg                                 <  1:426465/149‑1 (MQ=255)
                                                                                                                     taaGTATAAACAAGTTTAAGAAGTTATTTAAAACTCACTTCTTATCTATACAACTTTATCTCTTTACTGAATTAGTTATAGTTTCAGTATCTATATCTTTTACCGTATATGTTTGGGATACTATTTGTATAGATTTATGATGATTCggg                                <  2:621990/149‑1 (MQ=255)
                                                                                                                      aaGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCgggg                               >  1:1487504/1‑149 (MQ=255)
                                                                                                                        gAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACAGTATATGTTTGGGATACTATTTGTATAGATTTATGATGAATCgggtaa                             >  2:362472/1‑146 (MQ=255)
                                                                                                                            aaaCAAGTTTAAGAAGTGAGTTATAACTAACTTATTATCTATACAACTTAATCTCTTTACTGGATTAGTTAAAGTTTCCTTATCGATATCTTTTACCGTATATGTTGGGGATACTAGTTGTATAGATTTATTTTGATTCgggggcgata                         >  2:1494327/1‑143 (MQ=255)
                                                                                                                             aaCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGTGACCAAAt                        >  1:841729/1‑149 (MQ=255)
                                                                                                                              aCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAAtt                       <  2:1487504/149‑1 (MQ=255)
                                                                                                                               cAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTAGGGGACCAAATTa                      >  1:1321904/1‑149 (MQ=255)
                                                                                                                                 aGTTTAAGAAGTGAGATAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTaaa                    <  2:856245/149‑1 (MQ=255)
                                                                                                                                  gTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAAt                   <  1:1567796/149‑1 (MQ=255)
                                                                                                                                  gTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAAt                   <  2:174608/149‑1 (MQ=255)
                                                                                                                                   tttAAGAAGTGAGTTAAAACTCACTTATTATATATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATATTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATg                  <  2:838877/149‑1 (MQ=255)
                                                                                                                                    atAAGAAGTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGc                 >  1:1349837/2‑149 (MQ=255)
                                                                                                                                     tAAGAAGTTATGTAAAACTCACTTATTATCTATACAACTTAATCTCTTTACTTAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCg                <  2:665318/149‑1 (MQ=255)
                                                                                                                                     tAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCg                >  2:131365/1‑149 (MQ=255)
                                                                                                                                     tAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCg                <  1:15358/149‑1 (MQ=255)
                                                                                                                                      aagaagTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCTATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGa               <  2:1327845/149‑1 (MQ=255)
                                                                                                                                       agaagTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTTCTGAATTAGTTAAAGTTTCAGTATCGAAATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTTTGATGATTCGGGGACCAAATTAAATGCGAg              >  2:204832/1‑149 (MQ=255)
                                                                                                                                        gaagTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAgg             <  2:199406/149‑1 (MQ=255)
                                                                                                                                           gTGAGTTAAAACTCACTTCTTATCTATACAACTAAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGTATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGat          >  2:1293886/1‑149 (MQ=255)
                                                                                                                                            tGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGata         <  2:1329208/149‑1 (MQ=255)
                                                                                                                                             gAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTAACGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATTAGAGGGatat        >  1:196447/1‑149 (MQ=255)
                                                                                                                                                   aaaCTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGATTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCGGa  <  1:1023378/149‑1 (MQ=255)
                                                                                                                                                   aaaCTCACTTCTTATCTATAAAACTTAATATCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGTGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAAATAAATGCGAGGGATATTCCGGa  <  1:879111/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCGGA  >  NC_000913/3798088‑3798383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: