Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,891,502 C→G 5.1% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,5020CG 5.1% 315.4 / 9.1 117intergenic (+19/‑113)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (62/48);  new base G (2/4);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

Only 100 of 123 total aligned reads displayed.

GAAGTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGT  >  NC_000913/3891354‑3891649
                                                                                                                                                    |                                                                                                                                                   
gaagTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAagcccgcc                                                                                                                                                     >  1:470454/1‑149 (MQ=255)
         cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCAATCGCTgg                                                                                                                                            >  2:711936/1‑149 (MQ=255)
          tttcttaTGTTCTCGGTCTGGGGAGGTATTCTTATTCCGGCGATTGTCATTCTATTTGGTATAAACGTGATTTTGTGCTGGTTCGGCAAAGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                                                                           <  1:1017667/143‑1 (MQ=255)
          aaTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                           <  2:1278900/149‑1 (MQ=255)
          aaTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                           <  1:488689/149‑1 (MQ=255)
          aaTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGAGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                           >  2:1097874/1‑149 (MQ=255)
           aTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGct                                                                                                                                          >  2:711479/1‑149 (MQ=255)
            tCAGATGTGCACGGTCTGGGGCGGCTATCTTATTACGGAGATTGGCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGGCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                         <  1:1057728/149‑1 (MQ=255)
              aGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTTATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTGCAAGAAGCCAGCGAATGGCTGGcttct                                                                                                                                       <  2:373216/149‑1 (MQ=255)
              aGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttct                                                                                                                                       <  1:1206622/149‑1 (MQ=255)
              aGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGGGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttct                                                                                                                                       >  1:182216/1‑149 (MQ=255)
              aGATCTTCACGGTCTGGGGCGGCAAGCTTATTCGTGCGAGTGTCATTATATTTTGTATAACCGTGATTTTGTGCTCGTGGGGCATCGTCTTTAACGTGTTACCTAATTTTGGCTAAATCCTTAAAGAAGCCAGCCATTCGCTGGcttct                                                                                                                                       <  2:1099986/149‑1 (MQ=255)
               gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt                                                                                                                                      <  2:406579/149‑1 (MQ=255)
               gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCAGTCGCTGGcttctt                                                                                                                                      >  1:188482/1‑149 (MQ=255)
                  gTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTgaa                                                                                                                                   <  1:259665/149‑3 (MQ=255)
                   ttCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAGCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCct                                                                                                                                  <  2:827704/149‑1 (MQ=255)
                      aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCtctc                                                                                                                               >  1:1068929/1‑149 (MQ=255)
                      aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCtctc                                                                                                                               >  1:518947/1‑149 (MQ=255)
                      aCGGTCTGGGGCGGCAATCATATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCTGCAACGTCTTTAACGTGTTACCTAAATTTGGATAAATCCTTCAAGAAGCCAGCCATTCGATAGCTTCTAGCctcta                                                                                                                               >  1:645421/1‑148 (MQ=255)
                           cTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGaa                                                                                                                          <  1:526601/149‑1 (MQ=255)
                               ggcggcAATCTTATTCCGGCGATTGTCATTCTCTTTGTTATATACGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCTCTGGCTTCTTGCCTCTCAGGAAATCa                                                                                                                      <  2:563190/149‑1 (MQ=255)
                               ggcggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGAGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCa                                                                                                                      <  2:70488/149‑1 (MQ=255)
                                  ggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                    <  1:151676/148‑1 (MQ=255)
                                  ggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                    >  2:151676/1‑148 (MQ=255)
                                    cAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCTGGATTTTGTGCTGTTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                 <  1:899731/149‑1 (MQ=255)
                                     aaTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGTAATCACTTATg                                                                                                                >  1:1548648/1‑149 (MQ=255)
                                      aTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATga                                                                                                               >  2:829567/1‑148 (MQ=255)
                                       tCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                                                              <  2:1273260/149‑1 (MQ=255)
                                       tCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGATCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                                                              >  1:763437/1‑149 (MQ=255)
                                          tATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCaa                                                                                                           >  1:19684/1‑149 (MQ=255)
                                               cGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCATATGGc                                                                                                      >  2:1476329/1‑149 (MQ=255)
                                                ggCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCa                                                                                                     <  2:538304/149‑1 (MQ=255)
                                                 gCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTGTTTTACGTGTTACCTAAATTTGTCTATATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTAAGGAAATCACTTATGTCCAAAtttcaa                                                                                                    >  2:1037033/1‑144 (MQ=255)
                                                 gCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCaa                                                                                                    <  1:925981/149‑1 (MQ=255)
                                                  cGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAAc                                                                                                   <  1:1329011/149‑1 (MQ=255)
                                                    aTTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTc                                                                                                 >  1:823916/1‑149 (MQ=255)
                                                    aTTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTc                                                                                                 <  1:1512387/149‑1 (MQ=255)
                                                           ttCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGGTAACTAAATTTGTCTATCTCCTTCAAGAAGAAAGCCATTAGAGTGCGTATTGCCTCTAATGATATCACTGATGTCAAAAGTGCAACTCGCGTGAt                                                                                          >  1:1021474/1‑149 (MQ=255)
                                                              tctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcct                                                                                       <  1:341087/149‑1 (MQ=255)
                                                                   ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTc                                                                                  <  2:978367/149‑1 (MQ=255)
                                                                   ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTc                                                                                  <  1:661280/149‑1 (MQ=255)
                                                                   ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTc                                                                                  <  1:1166409/149‑1 (MQ=255)
                                                                    gTATACCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTAGCTAAATTTGGCTAAATCCTTCTAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCAGTGTTGTCCATATGGCAACTCGCCGGATcctactgga                                                                                 >  2:895322/1‑146 (MQ=255)
                                                                    gTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTca                                                                                 <  1:1241358/149‑1 (MQ=255)
                                                                    gTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTca                                                                                 >  1:426128/1‑149 (MQ=255)
                                                                    gTATAACCGTGATTTTGTGCGGGTTCGGCAACGTCGTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGGCCAAATGGCAACTCGCCTGATCCTCCTTca                                                                                 <  2:1115031/149‑1 (MQ=255)
                                                                      ataAGCGTGATTTTGTGCTGGTTCGGCATCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcacc                                                                               <  2:36494/149‑1 (MQ=255)
                                                                      ataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcacc                                                                               >  2:1138191/1‑149 (MQ=255)
                                                                      ataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcacc                                                                               >  1:1365395/1‑149 (MQ=255)
                                                                           ggTGATTTTGTGCTGGTTCTGCCACGTCTTTAACGTGTTACATTATTTTGTCTAAATCCTGCAAGAAGCCAGCCATTAGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCAGATCCTCCTTCACCACGTa                                                                          <  1:1057598/148‑1 (MQ=255)
                                                                                ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCtt                                                                     >  1:469113/1‑149 (MQ=255)
                                                                                 tttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCttt                                                                    <  1:1138191/149‑1 (MQ=255)
                                                                                     tgCTGGTTCGGCAACGTCTTTAAAGTGTAACCTAAATTTTGCTAAATCCTTCCTGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCCCGTATGCTTTGCGt                                                                <  2:286980/149‑1 (MQ=255)
                                                                                      gCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTATGCGTc                                                               >  2:727920/1‑149 (MQ=255)
                                                                                        tGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCAc                                                             >  2:449358/1‑149 (MQ=255)
                                                                                          gTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCCGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGGCCAAATGGCAACTCTCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCt                                                           >  2:1389209/1‑149 (MQ=255)
                                                                                          gTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCt                                                           >  2:1472469/1‑149 (MQ=255)
                                                                                          gTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCt                                                           >  1:1141184/1‑149 (MQ=255)
                                                                                             cGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTAc                                                        <  2:19684/149‑1 (MQ=255)
                                                                                               gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCTAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTa                                                      <  1:399340/149‑1 (MQ=255)
                                                                                               gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTa                                                      >  2:1240488/1‑149 (MQ=255)
                                                                                               gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTa                                                      >  1:724765/1‑149 (MQ=255)
                                                                                               gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCATACTa                                                      >  2:383308/1‑149 (MQ=255)
                                                                                               gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTGCGTGCCTCTCAGGAAAACACTAATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTa                                                      >  1:1280814/1‑149 (MQ=255)
                                                                                                 aaagtatttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGATGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATc                                                    <  2:935822/143‑1 (MQ=255)
                                                                                                 aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCCACTCGCATGATCCACCGTCACACCGTATGCTTTGCGGCACCTTACTTTc                                                    >  2:189103/1‑149 (MQ=255)
                                                                                                    gTCTGTAACGTGTTACCTAAAATTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCAGGCTTATTGCATCACAGGAAATAACTTATTACCAAATGGCAACTCGCCTGATCCTCCTTAACCACGACTGCTTGTCTTCACCTTAAAATCAgc                                                 >  2:1333867/1‑148 (MQ=255)
                                                                                                     tCTTTATCGTGGTACCTAAATTTGGCTAACTCCTTAAAGAAGCCAGCCATTCTCTGGCTTCTTGCCTCTCAGTAAATCACTTATGTCCAATTTGAAACTCGCCTGATCCCCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGa                                                <  2:645421/149‑1 (MQ=255)
                                                                                                     tCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGa                                                >  1:459699/1‑149 (MQ=255)
                                                                                                     tCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCGTTGCGTCACCTTACTATCAGGa                                                >  2:272748/1‑149 (MQ=255)
                                                                                                         tAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATTTCCAAATGGAAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCt                                            >  1:316866/1‑149 (MQ=255)
                                                                                                          aaCGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCtt                                           <  2:786069/149‑1 (MQ=255)
                                                                                                          aaCGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGACTCTCAGGAAATCAGTTATGTCCAAAGGGCAACTCGCCTGATCCTCCTTGACCACGAATGGTTTGCGTCACCTTACTATCAGGTCGGtt                                           >  1:317880/1‑149 (MQ=255)
                                                                                                             gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCCCCTTACTATCAGGACGCTTTAg                                        >  2:639896/1‑149 (MQ=255)
                                                                                                             gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAg                                        >  1:1561007/1‑149 (MQ=255)
                                                                                                              tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATGCTCCTTCACCACGTATGCTTTGCGTCACCATACTATCAGGACGCTTTAGc                                       >  2:1306704/1‑149 (MQ=255)
                                                                                                               gtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATGCTCCTTCACCACGTATGCTTTGCGTCACCTTACTAGCAGGACGCTTTAGcc                                      >  1:537289/1‑149 (MQ=255)
                                                                                                                ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGccc                                     <  1:933566/149‑1 (MQ=255)
                                                                                                                  aCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCTCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATAAGGACGCTTTAGCCCAt                                   >  1:1314759/1‑149 (MQ=255)
                                                                                                                   ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAATTCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCagg                                  >  1:430030/1‑147 (MQ=255)
                                                                                                                   ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCTTg                                  >  1:670533/1‑149 (MQ=255)
                                                                                                                   ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATg                                  <  1:711936/149‑1 (MQ=255)
                                                                                                                     tAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCCCGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTc                                >  1:677819/1‑149 (MQ=255)
                                                                                                                     tAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTc                                <  1:1427402/149‑1 (MQ=255)
                                                                                                                          ttGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCt                           >  1:99423/1‑149 (MQ=255)
                                                                                                                             gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCACCTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCtgtt                        >  2:915209/1‑149 (MQ=255)
                                                                                                                              cTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACGATCAGGTCGGTTTAGCCCATGTCCCGCttttt                       >  2:1550819/1‑149 (MQ=255)
                                                                                                                               tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTg                      >  2:749831/1‑149 (MQ=255)
                                                                                                                               tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTg                      >  1:756672/1‑149 (MQ=255)
                                                                                                                                     cTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGt                >  2:523915/1‑149 (MQ=255)
                                                                                                                                         atgaagCCAGCATTTCGCTGGATTCTTGGATCTCATTTAATTACTTATTTCCAATTGGCATCTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCTCCTTACTATCATGACGCTTTAGCCCATGTCCCGCTTTTTGATTTgttgtt            <  1:471657/147‑1 (MQ=255)
                                                                                                                                         aagaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTAAACACGTATGCTTTGGGTCACCTTACTATCAGGACGCTTTAGCCCATTACCCCCTTTTTGATTTGTAggt            >  2:1303537/1‑147 (MQ=255)
                                                                                                                                           gaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGtttt          >  2:1220693/1‑149 (MQ=255)
                                                                                                                                           gaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGtttt          >  2:1269140/1‑149 (MQ=255)
                                                                                                                                            aagACAGCGAATGGCTGGCTGCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTTATCCTCCTTCACCACGTATGCATTGCGGCACCTTACAATCAGGCCGATTTAGACCCTGACCCGCTTTTTGAATTGTAGTTTTg         >  2:1560791/1‑149 (MQ=255)
                                                                                                                                              gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGcc       <  2:220634/149‑1 (MQ=255)
                                                                                                                                                 agccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg    <  2:559658/149‑1 (MQ=255)
                                                                                                                                                  gccaTTCGCTGGCTTCTTGCCTCTCAGGAAATAACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTGCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGACCCGCTTTTTGATTTGTAGTTTTGCACTgg   >  1:555379/1‑149 (MQ=255)
                                                                                                                                                   ccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGt  <  1:1135723/149‑1 (MQ=255)
                                                                                                                                                   ccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGt  >  1:531071/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGT  >  NC_000913/3891354‑3891649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: