Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,915,520 T→A 5.6% intergenic (‑320/+33) glmU ← / ← atpC fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,915,5200TA 5.6% 275.8 / 2.2 95intergenic (‑320/+33)glmU/atpCfused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit
Reads supporting (aligned to +/- strand):  ref base T (47/42);  new base A (3/3);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAG  >  NC_000913/3915378‑3915668
                                                                                                                                               |                                                                                                                                                    
ccTTAATTTAAGCGGCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGAc                                                                                                                                                <  2:682788/149‑1 (MQ=255)
  ttAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTg                                                                                                                                              >  2:1508921/1‑149 (MQ=255)
  ttAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCACCCTTTTTCCAGACTg                                                                                                                                              >  2:1423223/1‑149 (MQ=255)
  ttAATTTAAGCGTCAGGTGGATGTTTTAGCTCTAATTTCGATCAATGAATAAACAGGAAAACAGGGGTGTTTTATATCAGCATGATCTATGTGAACGCAAGTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCGAGAcgg                                                                                                                                              >  2:177479/1‑147 (MQ=255)
    aaTTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGc                                                                                                                                            <  1:306085/149‑1 (MQ=255)
    aaTTTAAGCGTCAGCTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTCAACGCTAATCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGCCAGGc                                                                                                                                            >  2:659176/1‑149 (MQ=255)
       ttAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCttt                                                                                                                                         >  2:1524224/1‑149 (MQ=255)
          aGCGTCAGGTGTATGTTTTTGCTCTTATTTCGCTCAATGAATAAACAGGATAACACGGGGGTTTTATATCAGCAGGTTCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCTGCCTGTTTCCAGACTGGCTTTtgt                                                                                                                                      <  1:1173906/149‑1 (MQ=255)
           gCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTtgtg                                                                                                                                     <  1:721983/149‑1 (MQ=255)
            cGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAATCCAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCtttttttt                                                                                                                                    <  1:823349/149‑5 (MQ=255)
             gTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCt                                                                                                                                   >  2:1093343/1‑149 (MQ=255)
             gTCAGGTGGATGTTTTTCCTCTTATTTCGATCAATTAATAAACTGTAAAACAGGGGGGTTTTATATCAGCTGTATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCttttttttg                                                                                                                                   <  2:185025/149‑6 (MQ=37)
                aGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCtttt                                                                                                                                <  1:1439432/149‑1 (MQ=255)
                aGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCtttt                                                                                                                                <  1:583501/149‑1 (MQ=255)
                      aTGTTTTTGCTCTTATTTCGATCAATGACTAAACAGGACAACAGGGGGGTTTTATATCAGCACGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGcc                                                                                                                          <  2:881549/149‑1 (MQ=255)
                        gtgtttGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATAGCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCgg                                                                                                                        <  1:1111446/146‑1 (MQ=255)
                        gTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCgg                                                                                                                        <  1:368286/149‑1 (MQ=255)
                        gTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGATTTTGTGCTTTTCAAGCCgg                                                                                                                        >  2:309118/1‑149 (MQ=255)
                         tttttGCTCCTATTTCGCTCAATGACTCAACAGGACAACAGGGGGGTTTTATATCAGCAGGATCTCTGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGgt                                                                                                                       <  1:1067097/149‑1 (MQ=255)
                           tttGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGtt                                                                                                                    <  1:524323/149‑1 (MQ=255)
                            ttGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGtt                                                                                                                    <  1:748770/149‑1 (MQ=255)
                              gCTCTTATTTCGATCAATGAATAAACTGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATTTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCTGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCTGTGTTAc                                                                                                                  <  1:908489/149‑1 (MQ=255)
                                tctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACAt                                                                                                                >  1:1263056/1‑149 (MQ=255)
                                tctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACAt                                                                                                                >  2:106478/1‑149 (MQ=255)
                                tctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACAt                                                                                                                >  1:1362642/1‑149 (MQ=255)
                                      ttCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTAGATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGGTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCttt                                                                                                          >  2:846568/1‑149 (MQ=255)
                                            cAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGt                                                                                                    <  1:1317954/149‑1 (MQ=255)
                                             actGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTc                                                                                                   <  1:1117357/147‑1 (MQ=255)
                                               tGAATAAACACGAACACAGGGGGGTTTTATATCACCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCaa                                                                                                 <  2:24999/149‑1 (MQ=255)
                                                gAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAAc                                                                                                >  2:1521793/1‑149 (MQ=255)
                                                     aaCAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGAt                                                                                           >  2:233596/1‑149 (MQ=255)
                                                     aaCAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGACTGTTTCCAGACTGGCTTTTGTGCTATTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGAt                                                                                           >  1:961778/1‑149 (MQ=255)
                                                          gacaaCAGCGGTGTTTTATATCACCCTGATCTTTGTGAACGCGATTCAGGACGGGTCCCACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAcgc                                                                                      <  2:1060019/146‑1 (MQ=255)
                                                          gAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGACAGCTATTCTGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAcgc                                                                                      <  1:379031/149‑1 (MQ=255)
                                                          gAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAcgc                                                                                      >  1:1530449/1‑149 (MQ=255)
                                                           aaaaCAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTGTTTTGGTCAACTCGATAAcgcg                                                                                     >  2:933360/1‑149 (MQ=255)
                                                             aaCAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGCCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCa                                                                                   >  1:1419582/1‑149 (MQ=255)
                                                                    gggTTTTATATCAGAAGGATCTATGTGAACGCTATTCAGGACGGGGCACTCGCGCAAAAAAAAGCCAGCCTGTTTCGAGACTGGCTATTGTGCTTTTGAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTgcgc                                                                            <  2:1391156/149‑1 (MQ=255)
                                                                       ttttATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGGCTGTTTCCAGACTGGCTTTTGTGCTTATCAAGCCTGTGTTACATCGCTTTTTTGGTCAACTCGTTTACGCGCAGCTGCGCGAt                                                                         >  1:318070/1‑149 (MQ=255)
                                                                       ttttATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGAt                                                                         >  2:468639/1‑149 (MQ=255)
                                                                        tttATATCAGCAGGATCTATGTGAACGCTATTGAGGACGGGGCACACGCGCAAAAAAATGCCAGCCTGTTTCCACACTGGCTTTTGTGCTTTTCAAGCCGGTTTTACATCGCTTTTTTGGTCAAGTCGATAACGCGCAGCTGCGCGTTc                                                                        <  1:493687/149‑1 (MQ=255)
                                                                        tttATAGCACCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTATCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATc                                                                        <  1:1054793/149‑1 (MQ=255)
                                                                        tttAAATCAGCAGGATATATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTAAACTCGATAACGCGCAGCTGCGCGATc                                                                        <  1:87522/149‑1 (MQ=255)
                                                                            tatCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCtt                                                                    >  2:46937/1‑149 (MQ=255)
                                                                             atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGGCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCttt                                                                   <  2:309522/149‑1 (MQ=255)
                                                                              tCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCCGCCTGTTTCCAGACTGGCTTTTGTGCGTTTCAAGCCGGTGTTACATCGCTTTTTTGGTAAACTCGATAACGCGCAGCTGCGCGATCGCTTTg                                                                  >  2:1538667/1‑149 (MQ=255)
                                                                               cagcctgATCTATGTGCACGCTAATCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCGGCGCGATCGCTTTgg                                                                 <  2:321575/143‑1 (MQ=255)
                                                                                cgcagGATCTATGTGAACGCTATTCAGGACGGGTCCCACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGc                                                                <  2:673696/148‑1 (MQ=255)
                                                                                agcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGt                                                                                                    >  2:1081994/1‑113 (MQ=255)
                                                                                agcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGt                                                                                                    <  1:1081994/113‑1 (MQ=255)
                                                                                agcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTGTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGc                                                                >  1:562134/1‑149 (MQ=255)
                                                                                 gcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTATTCACGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAAGCGCAGCTGCGCGATCGCTTTGGcc                                                               >  1:341257/1‑149 (MQ=255)
                                                                                  cagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCa                                                              <  2:587010/149‑1 (MQ=255)
                                                                                    ggATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCATTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGt                                                            <  1:618655/149‑1 (MQ=255)
                                                                                     gATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGtt                                                           >  2:1196496/1‑149 (MQ=255)
                                                                                          aTGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCa                                                      >  1:1544457/1‑149 (MQ=255)
                                                                                          aTGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGCTCAACTCGATAACGCTCAGCTGCGCGATCGCTTTGGCCAGTTCCGCa                                                      >  2:1164226/1‑149 (MQ=255)
                                                                                           tgtgAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAg                                                     >  1:708744/1‑149 (MQ=255)
                                                                                             tgAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGAc                                                   >  1:1414141/1‑149 (MQ=255)
                                                                                             tgAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTATTCAAGCCGGTGTTACATCGCTTTTTTCGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGCCCAGTTCCGCAGAc                                                   >  1:179992/1‑149 (MQ=255)
                                                                                              gAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACg                                                  >  1:1305004/1‑149 (MQ=255)
                                                                                                   cTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCATACGCCTGa                                             <  1:5927/149‑1 (MQ=255)
                                                                                                    tATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAg                                            <  1:1537245/149‑1 (MQ=255)
                                                                                                     aTTCTGGACGGGTCACACGGGCAAAAAAAAGCCAGCCTGTTACCAGACTGGCTTTTGTGCTTTTCAAGCCGGAGTTACATCGCTTTTTTGGGCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGc                                           <  1:1187558/149‑1 (MQ=255)
                                                                                                     aTTCAGGACGGGGCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGc                                           <  2:9003/149‑1 (MQ=255)
                                                                                                      ttCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCATCTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCg                                          >  2:1252462/1‑149 (MQ=255)
                                                                                                         aGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGCCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTATTTGGTCAACTCGATAACGCGCAGCTGCGCGCTCGCTTGTGCCAGTTCCGCAGACGCCTGCGCGTaa                                       >  1:874047/1‑149 (MQ=255)
                                                                                                         aGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTATTGTGCTTATCAAGCCGGTGTTACATCGCTTATTTGGTCAACTAGAAAACGCGCCCCTGCTCGATACCTTTGGCCACTTCCGCACACGCATGAGCGTaa                                       >  1:91164/1‑149 (MQ=255)
                                                                                                          ggACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTTCTTTTCAAGCCGGTGGTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCAAGTTCCGCAGACGCCTGAGCGTact                                      >  1:865477/1‑147 (MQ=255)
                                                                                                          ggACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACAGGCGTTTGTGCTTTTCAAGCCGGGGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCAGCGCGATAGCTTTGGCCAGTTCCGAACAAGCCTGAGCGTaaa                                      >  2:1407372/1‑148 (MQ=255)
                                                                                                           gACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCACCTCGATAACGCGCCGCTGCGCGCTCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATc                                     >  1:1513199/1‑149 (MQ=255)
                                                                                                                 tCACACGCGCAAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTATTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGt                                >  1:311028/1‑149 (MQ=255)
                                                                                                                 tCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTc                               >  1:1401104/1‑149 (MQ=255)
                                                                                                                   acacGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGc                             >  1:959501/1‑149 (MQ=255)
                                                                                                                    cccgcgCTAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGcc                            <  1:134264/147‑1 (MQ=255)
                                                                                                                     acGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCg                           >  2:655123/1‑149 (MQ=255)
                                                                                                                       gcgcAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTg                         >  2:697044/1‑149 (MQ=255)
                                                                                                                          cAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTgaga                      >  1:825259/1‑149 (MQ=255)
                                                                                                                            aaaaaaaaGCAAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTATATGCTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTCagag                     >  2:637614/1‑149 (MQ=255)
                                                                                                                             cacaaaaGCCAGTCTGGAAACAGGCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgc                    >  1:769570/4‑149 (MQ=255)
                                                                                                                              acaaaaGCCAGTCTGGAAACAGGCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgct                   >  1:114743/3‑149 (MQ=255)
                                                                                                                                aaaaGCCAGTCTGGAAACAGGCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCTATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACTCCTGACCGTAATCTACGTCGCCGTGAGAgctgc                 >  2:263355/1‑149 (MQ=255)
                                                                                                                                aaaaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgctgc                 <  2:1414141/149‑1 (MQ=255)
                                                                                                                                   agccagccTGTTTCCTGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTGGATAACGCTCAGCTGCGCGATCGCTTAGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTaa              <  2:341257/149‑1 (MQ=255)
                                                                                                                                   agccagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTaa              <  1:731895/149‑1 (MQ=255)
                                                                                                                                    gccagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAt             <  1:1508921/149‑1 (MQ=255)
                                                                                                                                    gccagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAt             <  1:106478/149‑1 (MQ=255)
                                                                                                                                     ccagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAtg            >  2:1479921/1‑149 (MQ=255)
                                                                                                                                      cagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAtgt           >  1:998211/1‑149 (MQ=255)
                                                                                                                                      cagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTATTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAtgt           <  1:939249/149‑1 (MQ=255)
                                                                                                                                        gcgtGTTTCCAGACTGGCTTTTGTGCTTTTCACGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGc         <  1:924492/146‑1 (MQ=255)
                                                                                                                                        gccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGc         <  2:1401104/149‑1 (MQ=255)
                                                                                                                                         ccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGCCGCCTGAGCGGAATCTACGTCGCCGTGAGAGCTGCTAATGTGca        >  1:278830/1‑148 (MQ=255)
                                                                                                                                          cTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGctc       <  2:526495/149‑1 (MQ=255)
                                                                                                                                          cTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGctc       <  2:218270/149‑1 (MQ=255)
                                                                                                                                             tttCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTc    <  1:233596/149‑1 (MQ=255)
                                                                                                                                             tttCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCa   >  2:658895/1‑149 (MQ=255)
                                                                                                                                              ttCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCa   <  1:1093343/149‑1 (MQ=255)
                                                                                                                                               tCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCGTCAg  >  2:1383988/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAG  >  NC_000913/3915378‑3915668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: