Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,969,429 A→C 5.8% K133N (AAA→AAC wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4290AC 5.8% 316.2 / 3.6 122K133N (AAA→AACwzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base A (68/44);  new base C (5/3);  total (74/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

TCCGTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCC  >  NC_000913/3969281‑3969576
                                                                                                                                                     |                                                                                                                                                   
    ttCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCg                                                                                                                                                 >  1:771385/1‑149 (MQ=255)
        aaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCGGCAAACCGACTATTACCAACAGCGGATGGTGGGCAACAGCAAGGCCGATgc                                                                                                                                             >  1:1066270/1‑149 (MQ=255)
           aTGGCTTCTGCCGACCAACCATCGGTCTTGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGTCAACAGCAAAGCCGATgcggc                                                                                                                                          >  1:985285/1‑149 (MQ=255)
              gctgctCCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTGTTTATGCAACTGGCCTCGTGGGAGACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGCTGGTGGGCAACAGCAAAGCCGATGCGGCGtt                                                                                                                                       <  1:1410565/149‑1 (MQ=255)
                ttCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGATGCGGCGTTGc                                                                                                                                     >  1:638046/1‑149 (MQ=255)
                ttCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGc                                                                                                                                     >  2:47434/1‑149 (MQ=255)
                   tGCCGACCAACCATCGGTCATGCACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGGGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAACGCCGCATCGGCTTTGCTgt                                                                                                                                  <  2:760730/149‑2 (MQ=255)
                     ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                >  2:55028/1‑149 (MQ=255)
                      cGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCTCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                               >  2:1268787/1‑149 (MQ=255)
                       gACCAACCATCGGTCATGGACGAAGCCTATAAAGACTTTCTTATGCAACTGGCCTAGTGGGATACCAGCAGAGAGTTATGGATGCAAACCGACTATTAAAAACAGCGGATGGTGGACAACCGCAAAGCCGATGCGGCGTCGCTGGATGa                                                                                                                              >  2:396883/1‑149 (MQ=255)
                        accaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAATCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa                                                                                                                             >  2:591922/1‑149 (MQ=255)
                        accaaccaTCGCGAATGGAGGAAGCCTATAAAGAGTTTGTTTTGCATCTGGCCGCGTGGGATACCCGCAGCGAGTTCTGGCTGCAAACAGACTATTACAAACCGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttgccc                                                                                                                             <  2:252966/149‑7 (MQ=255)
                         ccaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaaa                                                                                                                            <  1:1168270/149‑1 (MQ=255)
                         ccaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaaa                                                                                                                            >  2:921791/1‑149 (MQ=255)
                         ccaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGGTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaaa                                                                                                                            <  2:901604/149‑1 (MQ=255)
                           acccaTCGGTCATGGACGCAGCCGATAAAGAGTGTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGCGTTCTGGCTGCAAACCGACTATTACAACCAGCGGATGGAGGGCAACAGCCAAGCCTATGCGGCGTAGCTGGATGAAATg                                                                                                                          >  1:852873/3‑149 (MQ=255)
                           aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACAGACTATTACAAACAGCGGATGGTGCGCAACAGCAAAGCCGATGAGGCGTTGCTCGATCAAATg                                                                                                                          >  1:465315/1‑149 (MQ=255)
                             ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGACTCGTGGGAAACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAAGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt                                                                                                                        <  1:232682/149‑1 (MQ=255)
                                 cGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaac                                                                                                                    <  2:787626/149‑1 (MQ=255)
                                  ggTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaaca                                                                                                                   <  1:921791/149‑1 (MQ=255)
                                   gTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaa                                                                                                                  >  1:1129743/1‑149 (MQ=255)
                                    tCATGGACGAAGCCTATAAATAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTAAAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaac                                                                                                                 >  1:648507/1‑149 (MQ=255)
                                       tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATc                                                                                                              >  2:146160/1‑149 (MQ=255)
                                          aCGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAg                                                                                                           >  1:5996/1‑149 (MQ=255)
                                           cGAAGCCTATAATGAGTTTGTTATGCAACGGGCCTCGTGGGATACCCGCAGCGAGGGCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAgg                                                                                                          <  2:1391915/149‑2 (MQ=255)
                                           cGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGAGACACGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATCCGGCGGTGCTGGTTGAAATGATTACCAACCTCCAGt                                                                                                          >  1:1079832/1‑149 (MQ=255)
                                            gAAGCCTAAAAAGAGGGGGCTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAACCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                         <  1:1001243/149‑1 (MQ=255)
                                             taGCCTATAAAGAGTTTGTTATGCAACAGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGttt                                                                                                        <  2:229765/148‑1 (MQ=255)
                                                ccTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTCAAACAGCGGATGGTGGGCAAAAGCAACGCCGATTCGGCGTTGCTCGATGAAATGATTAACAACAACCAGTGTATcc                                                                                                    >  1:1493788/1‑149 (MQ=255)
                                                   ttaAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATcccc                                                                                                  <  2:1050138/148‑1 (MQ=255)
                                                   ataAAGAGTTTGTTATGCAACTGGCCTCGTGGGTGACCCGGAGAGCGGTCAGGCTGCAAACCGCCGATTACAAACAGCGGAGGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGAGTAACAACATCCTGTTTATcccc                                                                                                  >  2:1026870/1‑149 (MQ=255)
                                                   ataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCt                                                                                                                                    <  1:880769/115‑1 (MQ=255)
                                                    taAAGAGTTTGTTATGCAACTGGCCACGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCg                                                                                                 >  1:245307/1‑149 (MQ=255)
                                                     aaaGAGTTTGTTATGCCACTGGCCTCGTGGGATACCCGCAGAGCGTTCTGTCTGCAACCCGACCATTACACCCAGCGGAAGGTGGGCACCGGCAAAGCCGATGCGGCGTTTCTGGATGAAATGATTAACACCATCACGGTGATCCCCgg                                                                                                >  1:107812/1‑149 (MQ=255)
                                                     aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACACCATCCAGTTTATCCCCgg                                                                                                >  1:1142173/1‑149 (MQ=255)
                                                     aaaGAGTTTGTTATGCAACTGGCATCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACAGACTATTACAAAAAGCTGAGGGTGGGCAACAGCAAAGCCTATGCGGCGTTGCTGGATGAAAGGATTAACAACCTCCAGTTTATACCCgg                                                                                                >  2:113429/1‑149 (MQ=255)
                                                      aaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAATCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                               >  1:201198/1‑149 (MQ=255)
                                                      aaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                               >  2:61641/1‑149 (MQ=255)
                                                        gagTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAAGAACATCCAGTTTATCCCCGgaga                                                                                             >  1:1263748/1‑149 (MQ=255)
                                                         agTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTCGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGAc                                                                                            >  2:278244/1‑149 (MQ=255)
                                                           tttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACtt                                                                                          <  2:1281844/149‑1 (MQ=255)
                                                            ttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                         <  1:200559/149‑1 (MQ=255)
                                                            ttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                         <  2:316899/149‑1 (MQ=255)
                                                             tGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTTTTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACAGCCCGTTTATCCCCGGAGACTTTa                                                                                        >  2:501122/1‑149 (MQ=255)
                                                             tGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTa                                                                                        >  1:1423618/1‑149 (MQ=255)
                                                             cgTTATGCCCCTGGCCTCGTGGGACACCCGCAGCGAGTCCTGTCTGCAAACCGACTATTACACACAGCGGATGGTTGGCCACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTa                                                                                        <  1:1073351/148‑1 (MQ=255)
                                                                    cAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcgcgc                                                                                 <  2:665828/149‑1 (MQ=255)
                                                                     aaCTGGCCTCGTGGGATTCCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCACCAGCAAAGCCGAGGCGGCGTTGCTGGATGAAATGATTCACAACGTCCAGTGTATCCCCGGAGACTTTCCCcgcgcg                                                                                >  2:392957/1‑149 (MQ=255)
                                                                              cGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAc                                                                       >  1:552000/1‑149 (MQ=255)
                                                                               gTGGGATACCCGCAGAGAGTTCTGGCTGCATACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAAAATCCAGTTTATCCCAGGAGACTTTACCCGCGCGGTCAATGACa                                                                      <  2:1451849/149‑1 (MQ=255)
                                                                                  ggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                   >  2:1244935/1‑149 (MQ=255)
                                                                                   gATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGt                                                                  >  1:1510446/1‑149 (MQ=255)
                                                                                    aTACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTg                                                                 >  2:915005/1‑149 (MQ=255)
                                                                                     tACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGa                                                                <  1:1390136/149‑1 (MQ=255)
                                                                                       cccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAg                                                              <  1:871466/149‑1 (MQ=255)
                                                                                            agagagTTCTGGCTGCAAACCGACTTTTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTAt                                                         <  1:224490/149‑1 (MQ=255)
                                                                                            agagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTAt                                                         <  2:1510446/149‑1 (MQ=255)
                                                                                                agTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATGCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                     <  1:1376757/149‑1 (MQ=255)
                                                                                                  ttCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGa                                                   >  1:1238448/1‑149 (MQ=255)
                                                                                                    cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGaaa                                                 <  2:1182851/149‑1 (MQ=255)
                                                                                                     tGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCTGCGTTGCTTGTTGAAATGATTAAATACATCAAGTTTATCCACGGATACTTTACCCGCGCGTTCAATGACAGATTGAAGCTTATTGCAGAAAc                                                >  1:1017927/1‑149 (MQ=255)
                                                                                                          gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATAGCCGAAACCgcgc                                           >  1:1560193/1‑149 (MQ=255)
                                                                                                             aaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATACCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTg                                        >  2:1543273/1‑149 (MQ=255)
                                                                                                               ccGACTATTACAAACAGCGGATGGTTGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGGCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGAc                                      <  1:1501094/149‑1 (MQ=255)
                                                                                                               ccGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGAc                                      >  2:487889/1‑149 (MQ=255)
                                                                                                                cGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACg                                     >  2:703563/1‑149 (MQ=255)
                                                                                                                 gACTATTACAAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACg                                     >  2:576859/1‑149 (MQ=255)
                                                                                                                    tATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCtaa                                 >  2:262902/1‑149 (MQ=255)
                                                                                                                     aTTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCtaat                                <  1:1347745/149‑1 (MQ=255)
                                                                                                                         cAAACAGCGGATGGGGGGCAACCTCAAAGCCGATGCCGCGTTGCTGGATGTAATGATTAACAACCTCCATGTTATCCCCGGTGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                            <  1:1484608/149‑1 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGCCTTTCCCCGCGCGGGCATGGCCAGCGTGAAGCTTATTGCCGAACCCGCGCATGCGGGTAATAcccg                           >  1:1448412/1‑148 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGaaa                                                 >  1:1141464/1‑127 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGaaa                                                 <  2:1141464/127‑1 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCTCCTGACGCTAATAACCt                           >  1:764578/1‑149 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCt                           >  1:1213498/1‑149 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGACAATGACAGCGTGAAGCTTATTGCCGACACCGCGCCTGACGCTAATAACCt                           >  1:1076866/1‑149 (MQ=255)
                                                                                                                           aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTATATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCTCCTGACGCTAATAACCt                           >  1:295380/1‑149 (MQ=255)
                                                                                                                              cAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGtt                        <  2:552000/149‑1 (MQ=255)
                                                                                                                               aGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTa                       <  1:98447/149‑1 (MQ=255)
                                                                                                                                gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCATTGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                      <  1:557588/149‑1 (MQ=255)
                                                                                                                                gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                      <  2:427862/149‑1 (MQ=255)
                                                                                                                                  ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGt                    >  2:1151397/1‑149 (MQ=255)
                                                                                                                                  ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGGCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGt                    <  2:771385/149‑1 (MQ=255)
                                                                                                                                   gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGCAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                   >  1:1339345/1‑149 (MQ=255)
                                                                                                                                   gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGCAACCGCGCCTGACGCTAATAACCTGTTACGTc                   >  1:580660/1‑149 (MQ=255)
                                                                                                                                      ggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                <  1:278244/149‑1 (MQ=255)
                                                                                                                                      ggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                <  2:1423618/149‑1 (MQ=255)
                                                                                                                                        tggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTAt              >  2:356524/1‑149 (MQ=255)
                                                                                                                                         gggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATg             >  1:113810/1‑149 (MQ=255)
                                                                                                                                          ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGt            <  1:61641/149‑1 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCa                  >  2:796517/1‑142 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCa                  <  1:796517/142‑1 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt           <  1:915005/149‑1 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt           <  2:201198/149‑1 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt           >  2:1324212/1‑149 (MQ=255)
                                                                                                                                           gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt           <  1:1206893/149‑1 (MQ=255)
                                                                                                                                               cAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtt       <  2:62851/149‑1 (MQ=255)
                                                                                                                                                aGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt      >  2:951201/1‑149 (MQ=255)
                                                                                                                                                 gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGAGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtttt     >  1:1417452/1‑149 (MQ=255)
                                                                                                                                                    aaGCAGATGCGGCGTGGCTGGATGAAATGATTAACAACATCCAGTTTATCCCAGGAGACTTTACCCGCGCGGTCAATGACAGCGTTAAGCGTTTTGCAGAAACCGCGCCATAGGCTTATTACCTGGTAGGTGAGTAGTTTGAGTTTgcc  >  2:247219/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCCGTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCC  >  NC_000913/3969281‑3969576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: