Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,579 T→A 7.1% intergenic (‑36/+41) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5790TA 7.1% 251.5 / 5.5 94intergenic (‑36/+41)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base A (2/5);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCC  >  NC_000913/265431‑265726
                                                                                                                                                    |                                                                                                                                                   
cGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCt                                                                                                                                                     >  1:829861/1‑149 (MQ=255)
  gAAGTGACTGCCCTGGGCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGc                                                                                                                                                   <  1:1228114/149‑1 (MQ=255)
   acgTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCt                                                                                                                                                  <  2:766330/147‑1 (MQ=255)
          tGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTc                                                                                                                                           >  2:2899/1‑149 (MQ=255)
            cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGCGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCAAGCTGTCgg                                                                                                                                         >  2:692453/1‑149 (MQ=255)
             ccTGGTCGTCTTCGAGTGTGATCTGGCTGTACGTGGTCGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                        <  2:56767/149‑1 (MQ=255)
             ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCATCACGGATTGGGTCATTTTCGGGCGCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                        <  1:713651/149‑1 (MQ=255)
             ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                        <  2:1187565/149‑1 (MQ=255)
             ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                        <  1:401421/149‑1 (MQ=255)
             ccTGGTCGTCTTCGAGGGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                        <  1:1543464/149‑1 (MQ=255)
              cTGGTCGGCTTCGAGTGTGAAATTGCTGGACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAACGGCCCCGGTGGCAGCAACACGCATTGCGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTGGCTGTCgggg                                                                                                                                       <  2:743377/149‑1 (MQ=255)
                 gtcgtcTTCGAGTGTGATACTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGa                                                                                                                                    >  1:1440896/1‑149 (MQ=255)
                  gcgtcTTCGAGTGTGATATTGCTGTACGTTGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCCTTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGaa                                                                                                                                   <  2:1117662/148‑1 (MQ=255)
                   cgtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAt                                                                                                                                  <  2:498488/149‑1 (MQ=255)
                       ttCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                              >  2:942558/1‑149 (MQ=255)
                         cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGTATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                            <  1:1394416/149‑1 (MQ=255)
                         cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                            <  2:1536536/149‑1 (MQ=255)
                              gtgATATTGCTCTACGTGGTAGTGTCCGCTTGCGCTTGTCTCCGGGGGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGCCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGaa                                                                                                                       <  1:1082177/149‑1 (MQ=255)
                               tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAg                                                                                                                      >  1:943905/1‑149 (MQ=255)
                                   atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtac                                                                                                                  >  1:260371/1‑149 (MQ=255)
                                     tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                <  1:962664/149‑1 (MQ=255)
                                     tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCGCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAACCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                <  1:501974/149‑1 (MQ=255)
                                       cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                              >  1:460909/1‑149 (MQ=255)
                                       cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                              <  2:298711/149‑1 (MQ=255)
                                          gtcGTGTTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATc                                                                                                           <  1:291984/147‑1 (MQ=255)
                                                    tGACCGCTTGAGCTTGGCTCCGGGTGAAAGGCCCCTTTGGCAGCAACACGGTTTGGGTCATTTTCGGGCTCCTGATAAAACAAAACCCAGGCAGCCTGCTATCTGTCGTGGTGAATTTGCTGGGGAAGATACTACTATCAGGCGTtgct                                                                                                 <  1:178790/149‑1 (MQ=255)
                                                      aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                               >  1:591501/1‑149 (MQ=255)
                                                      aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                               >  2:1183322/1‑149 (MQ=255)
                                                       ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGTTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                              <  2:182810/149‑1 (MQ=255)
                                                       ccGCTTGCGCTTGGCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                              <  2:414699/149‑1 (MQ=255)
                                                         gcttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCACCACGGAGTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGt                                                                                            >  1:1004790/1‑149 (MQ=255)
                                                          cttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtc                                                                                           >  1:813151/1‑149 (MQ=255)
                                                            tgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCACCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtctc                                                                                         <  2:669211/149‑1 (MQ=255)
                                                             gcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAGAGAAAACCCCGGCAGCCGGCGAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTcc                                                                                        >  1:708628/1‑149 (MQ=255)
                                                             gcgcttgTCGCCGGGTGACAGGCCCCGGTGGCAGCAACACGGCTTGGGGCATTTTCGGGCTCCTGATAAAAGCAAACCCCGGCAGCCAGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGGTGCTGCAGTCTcc                                                                                        <  2:1396638/149‑1 (MQ=255)
                                                               gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCga                                                                                      >  2:1561238/1‑149 (MQ=255)
                                                                    tctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                                 >  1:620399/1‑149 (MQ=255)
                                                                    gctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                                 <  1:442185/148‑1 (MQ=255)
                                                                      tcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                               >  1:231888/1‑149 (MQ=255)
                                                                        cGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACa                                                                             <  2:829861/149‑1 (MQ=255)
                                                                         gggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAg                                                                            >  2:773528/1‑149 (MQ=255)
                                                                           gggAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                          <  1:380499/147‑1 (MQ=255)
                                                                           gTGAAAGGCCCCGGTGGCAGCAACACGGATGGGGTCATTTTCGGGCGCCTGATAACAGAAACCACCGGCCGCCAGCTCGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                          <  1:856161/149‑1 (MQ=255)
                                                                            tGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaaa                                                                         >  2:1496520/1‑148 (MQ=255)
                                                                            ggAAAGGCCCCGGTGGAAGCATCACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAAc                                                                         <  1:415261/148‑1 (MQ=255)
                                                                             gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACt                                                                        >  2:740859/1‑149 (MQ=255)
                                                                             gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGCCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTTCTGCAGTCTCCGAGAGTGGACAGAACt                                                                        >  2:490013/1‑149 (MQ=255)
                                                                              aaaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACtt                                                                       >  2:1360474/1‑149 (MQ=255)
                                                                                   ccccGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCATCCTGCTAGCTTTCGGGGTGAATATGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTGTCTCa                                                                  >  2:392614/1‑149 (MQ=255)
                                                                                        gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTACCTGTCGGCGTGAATTTGCTGGGGAAGCTACTACTATCAGTCGTTGCTGCAGTCTCCGAGCGTGGACAGCACTTTCTCAGCGtt                                                             >  1:1051286/1‑149 (MQ=255)
                                                                                              gctccccGGATTGGGGCATTTTCGGGCGCCTGATAAACGCAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCg                                                       <  2:60728/143‑1 (MQ=255)
                                                                                                 acacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTc                                                    <  2:1506924/149‑1 (MQ=255)
                                                                                                  cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGCCAGACCTTTCTCAGCGTTCTTCCGGTcc                                                   >  2:848036/1‑149 (MQ=255)
                                                                                                  cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTcc                                                   <  2:599465/149‑1 (MQ=255)
                                                                                                     ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTTTCGGGGTCAATATGCTGGGGAAGATACAACTATCAATCGTTTCTGCAGTCTCCGACAGTGGACAGAACATTCTCAGCGTTCTTCCgcacagcc                                                >  2:1452361/1‑142 (MQ=255)
                                                                                                     ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                                >  1:314936/1‑149 (MQ=255)
                                                                                                     ggATTGGGCCATTTTCGGGCGCCTGATAAAATAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCCTTGCTGCAGTCTCCGAGATTGGACTGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                                <  2:1174771/149‑1 (MQ=255)
                                                                                                        ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGATGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                             >  1:963019/1‑149 (MQ=255)
                                                                                                        ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                             <  1:223745/149‑1 (MQ=255)
                                                                                                          gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCTCAGTGaa                                           <  2:616099/149‑1 (MQ=255)
                                                                                                          gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCACAGCGTTCTTCCGGTCCGCAGTgca                                           >  2:1349865/1‑147 (MQ=255)
                                                                                                           gggcATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                          <  2:1175254/146‑1 (MQ=255)
                                                                                                           ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                          >  2:774560/1‑149 (MQ=255)
                                                                                                           ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                          >  2:476549/1‑149 (MQ=255)
                                                                                                           ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                          >  2:836620/1‑149 (MQ=255)
                                                                                                           ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                          >  1:1480350/1‑149 (MQ=255)
                                                                                                               cttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcc                                      <  2:957383/148‑1 (MQ=255)
                                                                                                                ttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAACAGTCGTTGCTGCAGTCGCCGAGAGTGGCCAGACGTTTGTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                                     >  1:488286/1‑149 (MQ=255)
                                                                                                                ttttCGGGCTCCTGATAAAAGAAAACCACGGCAGCCTGCTAGCTGTCGGGGGGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGTGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                                     >  1:868041/1‑149 (MQ=255)
                                                                                                                 tttCGGGCTCCTGATAAAAGAAAACCCCGTCCGCCTGCTAGCTGTCGGGGTGAATTTTCTGGGGAAGATACGACTTTCAGTCGTTGCTGCAGTCTCTGAGAGTGGGCAGCACGTTCTCAGCGTTCGTCCGGTCCGCAGTGAAGGTCCCt                                    >  2:1237896/1‑149 (MQ=255)
                                                                                                                   tCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGc                                  <  1:1426041/149‑1 (MQ=255)
                                                                                                                       gCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtg                              >  2:1062958/1‑149 (MQ=255)
                                                                                                                        cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                             >  1:21918/1‑149 (MQ=255)
                                                                                                                        cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                             <  2:604664/149‑1 (MQ=255)
                                                                                                                         tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                            <  2:260371/149‑1 (MQ=255)
                                                                                                                          ccTGAGAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                           >  1:327430/1‑149 (MQ=255)
                                                                                                                           cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtgtg                          >  2:1404951/1‑147 (MQ=255)
                                                                                                                           cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTAATATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCGCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                          >  2:846774/1‑149 (MQ=255)
                                                                                                                            tGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAGCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTc                         <  2:267818/149‑1 (MQ=255)
                                                                                                                             gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCa                        <  1:2899/149‑1 (MQ=255)
                                                                                                                               aaaaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAtt                      <  2:620989/148‑1 (MQ=255)
                                                                                                                                  aaGAAAACCCTGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGATCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                   >  1:483090/1‑149 (MQ=255)
                                                                                                                                  aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                   >  1:1198436/1‑149 (MQ=255)
                                                                                                                                   cgAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTCTCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGTACTTTCTCAGCGTTCTTCCGGGCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACg                  <  2:952455/148‑1 (MQ=255)
                                                                                                                                   aGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGACTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg                  >  2:912286/1‑149 (MQ=255)
                                                                                                                                    gAAAACCCCGGCAGCCTGCTTGCTGTCGGGGTGAATTTGCTGGGGAAGATTCTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt                 >  1:1422935/1‑149 (MQ=255)
                                                                                                                                       aaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGACCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACTTAGa              >  1:698981/1‑149 (MQ=255)
                                                                                                                                        aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGCACTTTCACAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc             >  1:1014540/1‑149 (MQ=255)
                                                                                                                                        aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc             >  2:1471458/1‑149 (MQ=255)
                                                                                                                                         ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTACCGGTCCGCAGTGAAGGTCCCTGATTAGTGGTCATTGACGTAGCCg            >  2:1032556/1‑149 (MQ=255)
                                                                                                                                          cccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt           >  1:923105/1‑149 (MQ=255)
                                                                                                                                            cGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg         >  2:978915/1‑149 (MQ=255)
                                                                                                                                              gccgCCTGCTAGCTGTCGGGGAGAATTTGCTGGGGAAGATACTAGTATCAGGCGTTGCTGCAGTCTCCGAGAGAGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGaa       <  1:388201/146‑1 (MQ=255)
                                                                                                                                                aGCCTGCTAGCTGTCGGGGTGAATTTGCTTGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACt     <  1:1212693/149‑1 (MQ=255)
                                                                                                                                                   cTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGGCATTGACGTAGACGTCGAACTGcc  <  2:591501/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCC  >  NC_000913/265431‑265726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: