Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,118,830 G→A 5.1% intergenic (+70/+15) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,118,8300GA 5.1% 320.9 / 6.1 117intergenic (+70/+15)zapB/rraAFtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
Reads supporting (aligned to +/- strand):  ref base G (43/68);  new base A (3/3);  total (46/71)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

Only 100 of 118 total aligned reads displayed.

GAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCA  >  NC_000913/4118682‑4118977
                                                                                                                                                    |                                                                                                                                                   
      ccATCTGAAATAACAGCAGAACTGCTGGCAGGAACGTCTGCAGGCCCTGCTTGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                               <  1:496134/149‑1 (MQ=255)
         tCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCtt                                                                                                                                            >  2:558860/1‑149 (MQ=255)
          cTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGCGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCttt                                                                                                                                           <  1:796176/149‑1 (MQ=255)
           tGAAAGAAGAGCAGAACGGCTTGCAGGATCGACTGCAGGCCCTGCTGGGTCGCACGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTCCTGTGTAGGCCAAGAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCtttt                                                                                                                                          <  2:868344/149‑2 (MQ=255)
           tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                          <  2:210598/149‑1 (MQ=255)
              aacaaCAGCAGAACGGCTGGCACGAACGTCTGCAGGCCCTGATTGGTAGCATGTAATATTCCAGATTACGGTTCATCTTCAGCGTATTTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGTCACCTTGCGTTGCCTTTCTTa                                                                                                                                       <  2:1400093/146‑1 (MQ=255)
              aaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTa                                                                                                                                       <  1:143431/149‑1 (MQ=255)
               aGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttct                                                                                                                                      <  1:670370/149‑3 (MQ=255)
                   cagcagAACGGCTGGCAGGAACGTCTTCAGGCCCTGCTGGGGCGCATGGAAGAGGTCTGATTACGCGTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                                                                                                                  <  2:1227342/149‑1 (MQ=255)
                     gcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTGTTTAATGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTGTTATCATTCa                                                                                                                                >  2:166880/1‑149 (MQ=255)
                     gcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCa                                                                                                                                >  2:1130620/1‑149 (MQ=255)
                     gcagAACGGCGGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGTCACCTTGCGGTGCCTTTCTTATCATTCa                                                                                                                                <  1:486221/149‑1 (MQ=255)
                         aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAACTTGCGGTGCCTTACTTATCATTCAataa                                                                                                                            >  1:696562/1‑148 (MQ=255)
                           cGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                                                                                                                          >  2:53847/1‑149 (MQ=255)
                            ggCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                                                                                                                         <  1:546705/149‑1 (MQ=255)
                            ggCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                                                                                                                         >  2:834510/1‑149 (MQ=255)
                               tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                                                                                                                      <  2:445882/149‑1 (MQ=255)
                                  cAGGAACGTCTGCAGTCCCTGCTGGGTCGAATGGTAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                                                                                                                   <  1:1376184/149‑1 (MQ=255)
                                  cAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                                                                                                                   <  1:464000/149‑1 (MQ=255)
                                   aGGAACGTCTGCAGGCCCTGCTGGGTCACATGGAAGAGGTCTCAATACGCTTCAACTTCCGCCTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAAAAAAAAGGCACCTTGCGGTGCCTATCTAATCAATCAAAATCCAGcctagc                                                                                                                  >  2:1469111/1‑144 (MQ=255)
                                     gAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                                                                                                                <  1:252625/149‑1 (MQ=255)
                                      aaCGTATGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGAAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttc                                                                                                               <  1:1078062/149‑1 (MQ=255)
                                       aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct                                                                                                              <  2:888438/149‑1 (MQ=255)
                                        cGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTg                                                                                                             <  2:250824/149‑1 (MQ=255)
                                         gTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGa                                                                                                            >  1:14949/1‑149 (MQ=255)
                                          tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa                                                                                                           >  1:1124640/1‑149 (MQ=255)
                                          gcggCAGGCCCTGCGGGGTCGCATGAAGACGTCTGATTACGCGTCAGCATCCGCGTAATTTCTTTCCTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa                                                                                                          <  2:1433701/146‑1 (MQ=255)
                                           cTGCAGGCCCTGCTGGGTCGCATGGAAGCGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa                                                                                                          <  2:1061185/149‑1 (MQ=255)
                                           cTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa                                                                                                          <  1:1011346/149‑1 (MQ=255)
                                            tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                         <  1:250887/149‑1 (MQ=255)
                                            cgCAGCCCCTGCTGGGTCGCATGGAAGAGCTCTGCTTCCGCGTCATCTTCCGCGGAATTGCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAACGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                         <  2:327261/148‑1 (MQ=255)
                                             gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTGTCATTCAATATCCAGCGGATCTTCTGAAAGa                                                                                                        <  1:1294075/149‑1 (MQ=255)
                                                 gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                    <  1:72392/149‑1 (MQ=255)
                                                  cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat                                                                                                   >  1:473125/1‑149 (MQ=255)
                                                    ctgctgGGTCGCATGGAAGATGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATcc                                                                                                 <  2:595504/149‑1 (MQ=255)
                                                       ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                              <  2:816768/149‑1 (MQ=255)
                                                        tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGt                                                                                             <  1:1566657/149‑1 (MQ=255)
                                                        tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGt                                                                                             <  2:1155271/149‑1 (MQ=255)
                                                            tCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTg                                                                                         >  2:925198/1‑149 (MQ=255)
                                                             cGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGt                                                                                        <  2:1509742/149‑1 (MQ=255)
                                                              gCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTc                                                                                       <  2:750016/149‑1 (MQ=255)
                                                               cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAATCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg                                                                                      >  2:1178852/1‑149 (MQ=255)
                                                               cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAAATTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg                                                                                      >  2:664128/1‑149 (MQ=255)
                                                                aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCgg                                                                                     <  1:568285/149‑1 (MQ=255)
                                                                  ggAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                                   <  1:1360844/149‑1 (MQ=255)
                                                                   gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCAt                                                                                  >  2:1342439/1‑149 (MQ=255)
                                                                     agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATaa                                                                                <  1:1028912/149‑1 (MQ=255)
                                                                       agGTCTGATTACGCTTCATCTGCCGCGTAATTTCATTACTGTGTAGGCCAGCAGGTAACGCAGAAAAAAGGCACCTAGAGGTGCCTTTCGTATCATTCAATATCCACCGGATCTTCTGAAAGAATAATCCCGGTATTCTCGGCATAAAg                                                                              >  1:342696/1‑149 (MQ=255)
                                                                        ggTCTGATTACGCCTCATCTTCCGCGTAATTTCTTAACTGTGTAGGCCAACAGGTAACGCAGAAAAAACGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGa                                                                             <  2:289102/149‑1 (MQ=255)
                                                                          tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATg                                                                           <  2:445368/149‑1 (MQ=255)
                                                                          tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATg                                                                           <  2:1442200/149‑1 (MQ=255)
                                                                           cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCCGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATgg                                                                          <  1:1177626/149‑1 (MQ=255)
                                                                            tGATTACGCTTCATCTTCCGCGTAATTTCTATACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGt                                                                         <  1:103906/149‑1 (MQ=255)
                                                                             gATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCAAGCGGATCTTCTGAAAGATTAATCCCGGTATTGTCGGCATAAAGATGGTc                                                                        >  2:603819/1‑149 (MQ=255)
                                                                              aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTGCTGAAAGAATAATCCCGGTATTGTCGGGATAAAGATGGTCg                                                                       >  2:1483155/1‑149 (MQ=255)
                                                                                 aCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCg                                                                    <  1:570121/149‑1 (MQ=255)
                                                                                 aCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCg                                                                    <  1:1349515/149‑1 (MQ=255)
                                                                                   gCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGAGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAAAATCCCGGTATTGTCGGCAAAAAGATGGGCGCAGga                                                                  >  1:855726/1‑149 (MQ=255)
                                                                                    cTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGAAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGTTGGTCGCCGgag                                                                 <  1:400105/149‑1 (MQ=255)
                                                                                      tCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGagaa                                                               <  1:834510/149‑1 (MQ=255)
                                                                                        aTCTTAGGCGTAATTTATTTACTGTGTAGGCCAACAGGTAACGCAGAAAAACTGAACCTTGCGGTGCCTTTCTTATCATTCTATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGagaaga                                                             <  1:919515/149‑1 (MQ=255)
                                                                                          cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                           <  2:471047/149‑1 (MQ=255)
                                                                                            tCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGGAGAAAAAAGGCACCTTGCGGTTCCTTTCTTATCATTCAATATCCATCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                         <  2:1436202/149‑1 (MQ=255)
                                                                                            tCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                         <  2:598595/149‑1 (MQ=255)
                                                                                              cgcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGa                                                       <  2:1149101/149‑1 (MQ=255)
                                                                                               gcgTAATTTCTTTACTGTGTAGGCCAACAGGAAAAGGAGAAAAAAGGCACCTTGCGGTGCCTATCTTATCATTCAAAATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGac                                                      >  2:76284/1‑149 (MQ=255)
                                                                                                  tAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGGCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATAACGGTATTGAAGTCATAAAGATGGTCGCCGGAGAAGAAGGTGACAcc                                                   >  1:1178272/1‑149 (MQ=255)
                                                                                                  tAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACAcc                                                   >  1:1195300/1‑149 (MQ=255)
                                                                                                    aTTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGc                                                 <  2:701408/149‑1 (MQ=255)
                                                                                                        cTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCaaa                                             >  1:470400/1‑149 (MQ=255)
                                                                                                         tttACTGTTTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGCATCTTCTGAAAGAATAAACCCGTTATTGTCGGCATAAAGATGGGCGCCGGAGAAGAAGGTGACACCGCCaaaa                                            <  1:1469111/149‑1 (MQ=255)
                                                                                                         tttACTGGGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCATTATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGGGACACCGCCaaaa                                            <  1:1344658/149‑1 (MQ=255)
                                                                                                          ttACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAt                                           >  2:582991/1‑149 (MQ=255)
                                                                                                            agtgtgTAGGCCTACAGGTATCTAATAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGCATCTTCTGAAAGAATAATCCCTGAATTGGCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTg                                         <  2:1298163/147‑1 (MQ=255)
                                                                                                            aCTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTg                                         <  1:1483574/149‑1 (MQ=255)
                                                                                                             cTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGa                                        <  1:926045/149‑1 (MQ=255)
                                                                                                             cTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGa                                        <  1:57994/149‑1 (MQ=255)
                                                                                                              tgtgTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTTACTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAATAATAATCCCGGTATTGTCGGCATAATGATGGTCGACGCATAAGAATGTTACACAGCCAAAATTGAc                                       >  2:1364703/1‑149 (MQ=255)
                                                                                                                 gtAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgcg                                    <  1:53847/149‑1 (MQ=255)
                                                                                                                     gCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCATCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAATATGGTCGCCGGAGAAGAACGTGACACCGCCAAAATTGACGCGGACa                                >  2:931350/1‑149 (MQ=255)
                                                                                                                       cAACAGGTAACGCAGAAACAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATCTCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATc                              <  2:406551/149‑1 (MQ=255)
                                                                                                                       cAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAATCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATc                              <  2:412285/149‑1 (MQ=255)
                                                                                                                        aaCAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCg                             <  2:320868/149‑1 (MQ=255)
                                                                                                                        aaCAGGTAACGCAGAAAAAAGGCACCTAGCGGTGCCTTTCATATCATTCAATATCCAGCGGATCTTCTGAAAGCATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGCAGCAGGTGACCCCGCCAAAATTGACGCGGACATCg                             >  2:491591/1‑149 (MQ=255)
                                                                                                                        aaCAGGTAACGCAGAAAAAACGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAATGTCGGAATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCAGACATCg                             >  1:504398/1‑149 (MQ=255)
                                                                                                                             gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                        >  2:369909/1‑149 (MQ=255)
                                                                                                                             gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGGGACACCGCCAAAATTGACGCGGACATCGCTTTc                        <  1:925198/149‑1 (MQ=255)
                                                                                                                              tAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                       <  1:1114580/149‑1 (MQ=255)
                                                                                                                                  gCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGCCACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                   >  1:778388/1‑149 (MQ=255)
                                                                                                                                  gCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                   >  2:1002160/1‑149 (MQ=255)
                                                                                                                                   cAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAAt                  >  1:218897/1‑149 (MQ=255)
                                                                                                                                    aGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATg                 >  1:1238230/1‑149 (MQ=255)
                                                                                                                                      aagaaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGcc               >  2:1277383/4‑149 (MQ=255)
                                                                                                                                      aaaaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGcc               >  1:1363322/1‑149 (MQ=255)
                                                                                                                                       aaaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCt              >  1:27686/1‑149 (MQ=255)
                                                                                                                                         aaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTc            >  2:225084/1‑149 (MQ=255)
                                                                                                                                           aGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGc          <  2:1383820/149‑1 (MQ=255)
                                                                                                                                                ccTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCTGGATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTCACACCGCCAAAATTGACGCGGACATCGCGATCGCCAAAGCCTGCGCCAGCg     >  2:880584/1‑149 (MQ=255)
                                                                                                                                                ccTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCg     <  2:1529845/149‑1 (MQ=255)
                                                                                                                                                   tGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCa  <  1:1501525/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCA  >  NC_000913/4118682‑4118977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: