Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,223 A→T 6.3% I54N (ATT→AAT)  soxS ← superoxide response regulon transcriptional activator; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,2230AT 6.3% 290.8 / 4.8 104I54N (ATT→AAT) soxSsuperoxide response regulon transcriptional activator; autoregulator
Reads supporting (aligned to +/- strand):  ref base A (43/53);  new base T (5/2);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ATAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTT  >  NC_000913/4277075‑4277371
                                                                                                                                                    |                                                                                                                                                    
aTAATCGCTCGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATACCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGaa                                                                                                                                                      >  1:1083524/1‑149 (MQ=255)
 tAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCAGCCAGTAACAGGCGGCGTTGGCGAAt                                                                                                                                                     <  2:212211/149‑1 (MQ=255)
        tGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGCAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGACTGTAATCg                                                                                                                                              >  2:321565/1‑149 (MQ=255)
         gggAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCTGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGc                                                                                                                                             <  1:991330/149‑1 (MQ=255)
          ggAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGcc                                                                                                                                            >  1:553820/1‑149 (MQ=255)
           gAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCa                                                                                                                                           <  1:457379/149‑1 (MQ=255)
            cgTGCGATCAAACTGCCGACCGAAAACGCGGGAGAATGGCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCGCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACAATCGCCaa                                                                                                                                          <  1:1297029/148‑1 (MQ=255)
            aGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACATTCGCCaa                                                                                                                                          <  2:837637/149‑1 (MQ=255)
             gTGCGATCATACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAAGAGGCGGCGTTGGCGAATGTAATCGCCAAg                                                                                                                                         <  2:45802/149‑1 (MQ=255)
                 gATCAAACTGCCGACGGAAAACGCGGGAGAAGGACTGCTGCGCTACATAACCCCGGACCAATGCGATATCAAAAATCGGACGCTAGGTGGTGCGCAACTCAACGGCGGCCACTAACAGGCGGCGTAGGCGAATGTAATCGCCCAGCCTc                                                                                                                                     >  2:612134/1‑149 (MQ=255)
                  aTCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGCGACATATCCCAGGTCCCTTGCGATATCAAAATTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTCACAGGCGGCGTTGGCGAAGGTACTCGCCCAGCGGCt                                                                                                                                    >  2:1194530/1‑149 (MQ=255)
                  aTCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATCACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCt                                                                                                                                    >  1:872846/1‑149 (MQ=255)
                   tCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTg                                                                                                                                   >  2:1119192/1‑149 (MQ=255)
                    cAAACTGCCGACGGAAACCGCGGGAGAAGGTCTGCTGCGAGACATAACCCATGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGa                                                                                                                                  <  1:287085/149‑1 (MQ=255)
                    cAAACAGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGa                                                                                                                                  <  1:766284/149‑1 (MQ=255)
                     aaaCTGCCGACGGAAAACGCGGGAGACTGTCTGCTGCGATACATAACCCAGGTCCATTGCGAAATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCTGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGAt                                                                                                                                 <  1:894331/149‑1 (MQ=255)
                         tGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGCCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGACTGATGCga                                                                                                                             >  1:614460/1‑148 (MQ=255)
                           ccGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCa                                                                                                                           <  1:617755/149‑1 (MQ=255)
                            cGACGGAAAACGCGGGAGAAGGTCTGCTTCGAGACATAAACCAGGTCCATTGACATATAAAAAATCGGAGGCTCGGAGGTGCGAAACTCAACGCCGGCCAGAAACAGGCAGCGTTGGCGAAGGTAATAGACAATCGTCGGATGCGTCaa                                                                                                                          >  1:1052471/1‑148 (MQ=255)
                              aCGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCg                                                                                                                        >  2:1002716/1‑149 (MQ=255)
                                ggAACACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTg                                                                                                                      <  2:1465587/149‑1 (MQ=255)
                                 gAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTGAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGc                                                                                                                     >  1:512577/1‑149 (MQ=255)
                                      cgcgGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAc                                                                                                                <  1:357250/149‑1 (MQ=255)
                                       gcgGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCCCCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACa                                                                                                               >  2:1351271/1‑149 (MQ=255)
                                          ggAGCAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAACTCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTc                                                                                                            <  2:195424/149‑1 (MQ=255)
                                           gagaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCg                                                                                                           <  1:834091/149‑1 (MQ=255)
                                             gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  2:1367007/149‑1 (MQ=255)
                                             gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:744915/149‑1 (MQ=255)
                                             gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:1218856/149‑1 (MQ=255)
                                             caaGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATTTCAAAAATCGGACGCGCGGTGGTGCGCAAATCAACGGCGGCCAGTAACAGGCGGCTTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:1162791/148‑1 (MQ=255)
                                               aGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGc                                                                                                       >  2:750614/1‑149 (MQ=255)
                                                 gTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                     <  1:1479485/149‑1 (MQ=255)
                                                 gTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCGGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                     <  1:921392/149‑1 (MQ=255)
                                                  tCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAg                                                                                                    >  1:494785/1‑149 (MQ=255)
                                                         cGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACt                                                                                             >  1:317364/1‑149 (MQ=255)
                                                          gagaCATACCCCAGGTCCATTGCGATATCAAAAATCTGCCGCTCGGTGGTGCGCAACTCAACGTCGGCCAGTAACAGGCGGAGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACtt                                                                                            <  1:282933/149‑1 (MQ=255)
                                                           agaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCATGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACttt                                                                                           >  1:1443659/1‑149 (MQ=255)
                                                              cATACCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGaa                                                                                        <  1:455907/149‑1 (MQ=255)
                                                               aTAACCCAGGTCCATTGCGATATCAAAAATCGTACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAAt                                                                                       <  1:1372092/149‑1 (MQ=255)
                                                               aTAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACCGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAACTACCACATTGAAt                                                                                       >  1:83459/1‑149 (MQ=255)
                                                                tAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATa                                                                                      >  2:245260/1‑149 (MQ=255)
                                                                 aaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAg                                                                                     <  1:350363/149‑1 (MQ=255)
                                                                 aaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTATGATGCGTCACCGTGCGGAAACTTCGTTGCTAGTGACACTTTGAATAg                                                                                     >  2:919498/1‑149 (MQ=255)
                                                                  aCCCAGGTCCATTGCGATATCAAAATTCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTACCAGGCGGCGTTGGCGAATGTCATCGCCAAGCGTCTGATGCGTCCCCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                    <  2:282632/149‑1 (MQ=255)
                                                                  aCCCAGGGCCAATGAGCTATCAAAAAGCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                    <  1:1390610/149‑1 (MQ=255)
                                                                   cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAggc                                                                                   >  2:140424/1‑147 (MQ=255)
                                                                   cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCGTTGCAAGTACCACTTTGAGTAGcc                                                                                   >  2:855561/1‑149 (MQ=255)
                                                                    ccAGGTCCATTGCGCTATCATAAATCGGACGCTCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  <  2:587498/149‑1 (MQ=255)
                                                                    ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  <  2:594199/149‑1 (MQ=255)
                                                                    ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  >  2:569179/1‑149 (MQ=255)
                                                                     cAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTg                                                                                 <  2:1450367/149‑1 (MQ=255)
                                                                         tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                             >  1:45963/1‑149 (MQ=255)
                                                                         tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                             >  1:831000/1‑149 (MQ=255)
                                                                         tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACCCAACGGCGGCCAGTAACAGGCGGCGTTGTCGAATGTAATCGCCTAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCAATTTGAATAGCCTTAttt                                                                             >  1:771294/1‑149 (MQ=255)
                                                                            aTTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTTCAAGTACCACTTTGAATAGCCTGAtttttt                                                                          >  1:1293306/1‑149 (MQ=255)
                                                                             ttGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttttttt                                                                         >  1:1404080/1‑149 (MQ=255)
                                                                               gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                       <  1:474933/149‑1 (MQ=255)
                                                                               gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                       <  1:4688/149‑1 (MQ=255)
                                                                                 gttttCAAAAATCGGACGCTCGGTGGGGCGGAACTCTACGGCGGCCAGTATCCGGCGGCGTTGGCGTATGTAATGGCCAAGCGTGTGATGCGTCACCGTGCGGAACTTTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCgt                                                                     <  1:133065/145‑2 (MQ=255)
                                                                                 gATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGa                                                                     <  2:282095/149‑1 (MQ=255)
                                                                                     tCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTAc                                                                 <  2:677740/149‑1 (MQ=255)
                                                                                       aaaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                               >  1:1337289/1‑149 (MQ=255)
                                                                                        aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAGGCGTCTGATGCGTCACCGTGCGGAACATTCGGTGCAAGTCCCACTTTGAAGAGCCGGATTTTTTTGCGACTACaac                                                              >  2:496393/1‑147 (MQ=255)
                                                                                        aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCCAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGCGGAACATTCGTTCCAAGTAGCACTTTGAATAGCCTGAGTTTTTTGCGACTACATc                                                              >  1:185480/1‑149 (MQ=255)
                                                                                           ctccgACCCTCGGTTGGGCCCAACTCAAAGGCGCCGAGGACCCGCCGGCCTTGGCGAATGATATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                           <  1:1065057/145‑1 (MQ=255)
                                                                                           aTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                           <  1:355373/149‑1 (MQ=255)
                                                                                            tCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCTAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGTATAGCCTCATTTTTTTGCGACTACATCAATg                                                          <  2:1305822/149‑1 (MQ=255)
                                                                                              ggACGCTCGGTGGTGCGCAACTCATCGGCGGCCAGTAACATGCGGCGTTGGCGAATGTAATCGCCAAGCTTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGAGTACATCAATGtt                                                        <  1:612134/149‑1 (MQ=255)
                                                                                              ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGtt                                                        <  2:412255/149‑1 (MQ=255)
                                                                                                  gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                    >  2:1532202/1‑149 (MQ=255)
                                                                                                          gtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                            <  1:1315165/149‑1 (MQ=255)
                                                                                                          gtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                            <  1:1482318/149‑1 (MQ=255)
                                                                                                            gcgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCaa                                          <  1:1559105/149‑1 (MQ=255)
                                                                                                            gcgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCaa                                          >  1:315667/1‑149 (MQ=255)
                                                                                                             cgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAat                                         >  1:607149/1‑149 (MQ=255)
                                                                                                              gcAACTCTACGGAGGCCCGTAACAGGCGGCGTAGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAACGACCACTGTGAATAGCCTGAAATTTTTGCGCCTACATCAATGTTAAGCGGCTGGTCAata                                        >  2:1157351/1‑149 (MQ=255)
                                                                                                              gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAata                                        <  2:509764/149‑1 (MQ=255)
                                                                                                                 aCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGc                                     >  1:39935/1‑149 (MQ=255)
                                                                                                                  cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:435538/1‑149 (MQ=255)
                                                                                                                  cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:1343569/1‑149 (MQ=255)
                                                                                                                  cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:985642/1‑149 (MQ=255)
                                                                                                                   tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                   >  2:685252/1‑149 (MQ=255)
                                                                                                                   tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                   >  1:955565/1‑149 (MQ=255)
                                                                                                                        ggcggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGTCAATATGCTCGTCAAt                             <  2:553820/149‑1 (MQ=255)
                                                                                                                         gcggcCAGTAACAGGCGTCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAGATTCGTTTCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAAt                             <  2:940590/149‑1 (MQ=255)
                                                                                                                          cggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGATAAGCGGCTGGTCAATATGCTCGTCAATc                            <  2:872846/149‑1 (MQ=255)
                                                                                                                              cAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                        <  1:750614/149‑1 (MQ=255)
                                                                                                                              cAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGGCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                        <  1:114000/149‑1 (MQ=255)
                                                                                                                                gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCg                      >  2:1263616/1‑149 (MQ=255)
                                                                                                                                  aaCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                    >  2:132565/1‑149 (MQ=255)
                                                                                                                                   aCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATa                   >  2:62242/1‑149 (MQ=255)
                                                                                                                                       gctgcgTTGGCGTATGTAATCGCCAAGCGTCTGTTGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt               <  2:512577/146‑1 (MQ=255)
                                                                                                                                        cggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATc              <  1:522239/149‑1 (MQ=255)
                                                                                                                                          gcgTTGGCGAATGTAATCGCAAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAATATCCt            <  2:494785/149‑1 (MQ=255)
                                                                                                                                          cgcttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAACACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCTTCAATCCATGCGATAAGATACt            >  2:1198834/4‑149 (MQ=255)
                                                                                                                                            cttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGa          >  2:1453157/2‑149 (MQ=255)
                                                                                                                                             ttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaa         >  2:480316/1‑149 (MQ=255)
                                                                                                                                                gCGAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATTTGCTCGTCAATCGATGCGATAATATCCTGaataa      >  1:856393/1‑149 (MQ=255)
                                                                                                                                                    aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGGACATTCGTTGCAAGTACCACATTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt  >  1:159028/1‑149 (MQ=255)
                                                                                                                                                    aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt  <  2:413026/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTT  >  NC_000913/4277075‑4277371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: