Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,225 G→A 5.7% Y53Y (TAC→TAT) ‡ soxS ← superoxide response regulon transcriptional activator; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,2250GA 5.7% 276.5 / 6.2 104Y53Y (TAC→TAT) ‡soxSsuperoxide response regulon transcriptional activator; autoregulator
Reads supporting (aligned to +/- strand):  ref base G (46/52);  new base A (4/2);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 104 total aligned reads displayed.

ATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTT  >  NC_000913/4277078‑4277371
                                                                                                                                                   |                                                                                                                                                  
aTCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCAGTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGGAACAGGCGGCGTTGGCGAATGt                                                                                                                                                   >  2:522239/1‑149 (MQ=255)
     tGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGCAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGACTGTAATCg                                                                                                                                              >  2:321565/1‑149 (MQ=255)
      gggAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCTGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGc                                                                                                                                             <  1:991330/149‑1 (MQ=255)
       ggAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGcc                                                                                                                                            >  1:553820/1‑149 (MQ=255)
        gAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCa                                                                                                                                           <  1:457379/149‑1 (MQ=255)
         cgTGCGATCAAACTGCCGACCGAAAACGCGGGAGAATGGCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCGCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACAATCGCCaa                                                                                                                                          <  1:1297029/148‑1 (MQ=255)
         aGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACATTCGCCaa                                                                                                                                          <  2:837637/149‑1 (MQ=255)
          gTGCGATCATACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAAGAGGCGGCGTTGGCGAATGTAATCGCCAAg                                                                                                                                         <  2:45802/149‑1 (MQ=255)
              gATCAAACTGCCGACGGAAAACGCGGGAGAAGGACTGCTGCGCTACATAACCCCGGACCAATGCGATATCAAAAATCGGACGCTAGGTGGTGCGCAACTCAACGGCGGCCACTAACAGGCGGCGTAGGCGAATGTAATCGCCCAGCCTc                                                                                                                                     >  2:612134/1‑149 (MQ=255)
               aTCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGCGACATATCCCAGGTCCCTTGCGATATCAAAATTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTCACAGGCGGCGTTGGCGAAGGTACTCGCCCAGCGGCt                                                                                                                                    >  2:1194530/1‑149 (MQ=255)
               aTCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATCACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCt                                                                                                                                    >  1:872846/1‑149 (MQ=255)
                tCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTg                                                                                                                                   >  2:1119192/1‑149 (MQ=255)
                 cAAACTGCCGACGGAAACCGCGGGAGAAGGTCTGCTGCGAGACATAACCCATGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGa                                                                                                                                  <  1:287085/149‑1 (MQ=255)
                 cAAACAGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGa                                                                                                                                  <  1:766284/149‑1 (MQ=255)
                  aaaCTGCCGACGGAAAACGCGGGAGACTGTCTGCTGCGATACATAACCCAGGTCCATTGCGAAATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCTGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGAt                                                                                                                                 <  1:894331/149‑1 (MQ=255)
                    aCTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCGCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGc                                                                                                                               <  1:1438930/149‑1 (MQ=255)
                      tGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGCCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGACTGATGCga                                                                                                                             >  1:614460/1‑148 (MQ=255)
                        ccGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCa                                                                                                                           <  1:617755/149‑1 (MQ=255)
                         cGACGGAAAACGCGGGAGAAGGTCTGCTTCGAGACATAAACCAGGTCCATTGACATATAAAAAATCGGAGGCTCGGAGGTGCGAAACTCAACGCCGGCCAGAAACAGGCAGCGTTGGCGAAGGTAATAGACAATCGTCGGATGCGTCaa                                                                                                                          >  1:1052471/1‑148 (MQ=255)
                           aCGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCg                                                                                                                        >  2:1002716/1‑149 (MQ=255)
                             ggAACACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTg                                                                                                                      <  2:1465587/149‑1 (MQ=255)
                              gAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTGAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGc                                                                                                                     >  1:512577/1‑149 (MQ=255)
                                   cgcgGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAc                                                                                                                <  1:357250/149‑1 (MQ=255)
                                    gcgGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCCCCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACa                                                                                                               >  2:1351271/1‑149 (MQ=255)
                                       ggAGCAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAACTCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTc                                                                                                            <  2:195424/149‑1 (MQ=255)
                                        gagaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCg                                                                                                           <  1:834091/149‑1 (MQ=255)
                                          gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:1218856/149‑1 (MQ=255)
                                          gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  2:1367007/149‑1 (MQ=255)
                                          gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:744915/149‑1 (MQ=255)
                                          caaGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATTTCAAAAATCGGACGCGCGGTGGTGCGCAAATCAACGGCGGCCAGTAACAGGCGGCTTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                         <  1:1162791/148‑1 (MQ=255)
                                            aGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGc                                                                                                       >  2:750614/1‑149 (MQ=255)
                                              gTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                     <  1:1479485/149‑1 (MQ=255)
                                              gTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCGGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                     <  1:921392/149‑1 (MQ=255)
                                               tCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAg                                                                                                    >  1:494785/1‑149 (MQ=255)
                                                      cGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACt                                                                                             >  1:317364/1‑149 (MQ=255)
                                                       gagaCATACCCCAGGTCCATTGCGATATCAAAAATCTGCCGCTCGGTGGTGCGCAACTCAACGTCGGCCAGTAACAGGCGGAGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACtt                                                                                            <  1:282933/149‑1 (MQ=255)
                                                        agaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCATGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACttt                                                                                           >  1:1443659/1‑149 (MQ=255)
                                                           cATACCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGaa                                                                                        <  1:455907/149‑1 (MQ=255)
                                                            aTAACCCAGGTCCATTGCGATATCAAAAATCGTACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAAt                                                                                       <  1:1372092/149‑1 (MQ=255)
                                                             tAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATa                                                                                      >  2:245260/1‑149 (MQ=255)
                                                              aaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTATGATGCGTCACCGTGCGGAAACTTCGTTGCTAGTGACACTTTGAATAg                                                                                     >  2:919498/1‑149 (MQ=255)
                                                               aCCCAGGTCCATTGCGATATCAAAATTCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTACCAGGCGGCGTTGGCGAATGTCATCGCCAAGCGTCTGATGCGTCCCCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                    <  2:282632/149‑1 (MQ=255)
                                                               aCCCAGGGCCAATGAGCTATCAAAAAGCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                    <  1:1390610/149‑1 (MQ=255)
                                                                cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAggc                                                                                   >  2:140424/1‑147 (MQ=255)
                                                                cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCGTTGCAAGTACCACTTTGAGTAGcc                                                                                   >  2:855561/1‑149 (MQ=255)
                                                                 ccAGGTCCATTGCGCTATCATAAATCGGACGCTCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  <  2:587498/149‑1 (MQ=255)
                                                                 ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  >  2:569179/1‑149 (MQ=255)
                                                                 ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                  <  2:594199/149‑1 (MQ=255)
                                                                  cAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTg                                                                                 <  2:1450367/149‑1 (MQ=255)
                                                                      tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                             >  1:831000/1‑149 (MQ=255)
                                                                      tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                             >  1:45963/1‑149 (MQ=255)
                                                                      tCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACCCAACGGCGGCCAGTAACAGGCGGCGTTGTCGAATGTAATCGCCTAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCAATTTGAATAGCCTTAttt                                                                             >  1:771294/1‑149 (MQ=255)
                                                                         aTTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTTCAAGTACCACTTTGAATAGCCTGAtttttt                                                                          >  1:1293306/1‑149 (MQ=255)
                                                                          ttGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttttttt                                                                         >  1:1404080/1‑149 (MQ=255)
                                                                          ttGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGGCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttttttt                                                                         <  1:729618/149‑1 (MQ=255)
                                                                            gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                       <  1:4688/149‑1 (MQ=255)
                                                                            gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                       <  1:474933/149‑1 (MQ=255)
                                                                              gttttCAAAAATCGGACGCTCGGTGGGGCGGAACTCTACGGCGGCCAGTATCCGGCGGCGTTGGCGTATGTAATGGCCAAGCGTGTGATGCGTCACCGTGCGGAACTTTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCgt                                                                     <  1:133065/145‑2 (MQ=255)
                                                                                 atCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCCCTTTGAATAGCCTGATTTTTTTGCGACTa                                                                  >  1:430568/1‑149 (MQ=255)
                                                                                  tCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTAc                                                                 <  2:677740/149‑1 (MQ=255)
                                                                                    cacaaTCGGACGCTCCGCGGGCCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                               <  2:1083524/146‑1 (MQ=255)
                                                                                    aaaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                               >  1:1337289/1‑149 (MQ=255)
                                                                                     aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAGGCGTCTGATGCGTCACCGTGCGGAACATTCGGTGCAAGTCCCACTTTGAAGAGCCGGATTTTTTTGCGACTACaac                                                              >  2:496393/1‑147 (MQ=255)
                                                                                     aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCCAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGCGGAACATTCGTTCCAAGTAGCACTTTGAATAGCCTGAGTTTTTTGCGACTACATc                                                              >  1:185480/1‑149 (MQ=255)
                                                                                        ctccgACCCTCGGTTGGGCCCAACTCAAAGGCGCCGAGGACCCGCCGGCCTTGGCGAATGATATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                           <  1:1065057/145‑1 (MQ=255)
                                                                                        aTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                           <  1:355373/149‑1 (MQ=255)
                                                                                         tCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCTAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGTATAGCCTCATTTTTTTGCGACTACATCAATg                                                          <  2:1305822/149‑1 (MQ=255)
                                                                                           ggACGCTCGGTGGTGCGCAACTCATCGGCGGCCAGTAACATGCGGCGTTGGCGAATGTAATCGCCAAGCTTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGAGTACATCAATGtt                                                        <  1:612134/149‑1 (MQ=255)
                                                                                           ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGtt                                                        <  2:412255/149‑1 (MQ=255)
                                                                                               gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                    >  2:1532202/1‑149 (MQ=255)
                                                                                                       gtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                            <  1:1482318/149‑1 (MQ=255)
                                                                                                       gtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                            <  1:1315165/149‑1 (MQ=255)
                                                                                                         gcgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCaa                                          <  1:1559105/149‑1 (MQ=255)
                                                                                                         gcgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCaa                                          >  1:315667/1‑149 (MQ=255)
                                                                                                          cgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAat                                         >  1:607149/1‑149 (MQ=255)
                                                                                                           gcAACTCTACGGAGGCCCGTAACAGGCGGCGTAGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAACGACCACTGTGAATAGCCTGAAATTTTTGCGCCTACATCAATGTTAAGCGGCTGGTCAata                                        >  2:1157351/1‑149 (MQ=255)
                                                                                                           gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAata                                        <  2:509764/149‑1 (MQ=255)
                                                                                                              aCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGc                                     >  1:39935/1‑149 (MQ=255)
                                                                                                               cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:435538/1‑149 (MQ=255)
                                                                                                               cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:1343569/1‑149 (MQ=255)
                                                                                                               cTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                    >  1:985642/1‑149 (MQ=255)
                                                                                                                tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                   >  2:685252/1‑149 (MQ=255)
                                                                                                                tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                   >  1:955565/1‑149 (MQ=255)
                                                                                                                     ggcggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGTCAATATGCTCGTCAAt                             <  2:553820/149‑1 (MQ=255)
                                                                                                                      gcggcCAGTAACAGGCGTCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAGATTCGTTTCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAAt                             <  2:940590/149‑1 (MQ=255)
                                                                                                                       cggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGATAAGCGGCTGGTCAATATGCTCGTCAATc                            <  2:872846/149‑1 (MQ=255)
                                                                                                                           cAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                        <  1:750614/149‑1 (MQ=255)
                                                                                                                           cAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGGCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                        <  1:114000/149‑1 (MQ=255)
                                                                                                                             gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCg                      >  2:1263616/1‑149 (MQ=255)
                                                                                                                               aaCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                    >  2:132565/1‑149 (MQ=255)
                                                                                                                                aCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATa                   >  2:62242/1‑149 (MQ=255)
                                                                                                                                    gctgcgTTGGCGTATGTAATCGCCAAGCGTCTGTTGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt               <  2:512577/146‑1 (MQ=255)
                                                                                                                                     cggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATc              <  1:522239/149‑1 (MQ=255)
                                                                                                                                       gcgTTGGCGAATGTAATCGCAAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAATATCCt            <  2:494785/149‑1 (MQ=255)
                                                                                                                                       cgcttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAACACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCTTCAATCCATGCGATAAGATACt            >  2:1198834/4‑149 (MQ=255)
                                                                                                                                         cttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGa          >  2:1453157/2‑149 (MQ=255)
                                                                                                                                          ttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaa         >  2:480316/1‑149 (MQ=255)
                                                                                                                                             gCGAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATTTGCTCGTCAATCGATGCGATAATATCCTGaataa      >  1:856393/1‑149 (MQ=255)
                                                                                                                                                 aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGGACATTCGTTGCAAGTACCACATTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt  >  1:159028/1‑149 (MQ=255)
                                                                                                                                                 aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt  <  2:413026/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTT  >  NC_000913/4277078‑4277371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: