Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,060 C→T 38.9% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT38.9% 38.2 / 79.9 78intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base T (17/13);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-02

ATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC  >  NC_000913/4295917‑4296206
                                                                                                                                               |                                                                                                                                                  
aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                               <  2:1156517/149‑1 (MQ=32)
 tAAGGCGTTATTTCCGCATCCTACATGAACGCCTGATGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                              <  2:464887/149‑1 (MQ=11)
   aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                            >  2:1155694/1‑149 (MQ=17)
   aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                            <  1:577887/149‑1 (MQ=17)
    ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATGCGCCATCAACTCCTCATGc                                                                                                                                           >  1:549749/1‑149 (MQ=11)
     gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                          >  2:194425/1‑149 (MQ=17)
     gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg                                                                                                                                          <  1:541276/149‑1 (MQ=32)
     gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGATTATGTAGGTCGGATAAGGCGTATACGCCGCATCCGACAACAACGCCTGATgag                                                                                                                                          >  1:625200/1‑147 (MQ=255)
        tttATGCCGCATCCGACATCAACGCCTGATGCTACGATTAACGAGTCTTATCAGGCCTAAGACAGACAGAGCAATAGACTGATTTAGCGTGATGTTGTAGTTAGGATAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCgcgg                                                                                                                                       >  2:1099327/1‑146 (MQ=11)
        ttgaTGTCGGATGCGGCATAAACGCCTGATGCGACGCTTAACGCGTCTTATAAGGCCTACGCCAGCCAGCGCAATAGCCTGAGTTAGGGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACGTCAACGCCTGATGCGACg                                                                                                                                       >  1:1024422/4‑149 (MQ=11)
         ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                      <  1:1431334/149‑1 (MQ=17)
           aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATATCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                    <  2:941224/149‑1 (MQ=255)
            tGCCGCATCCTACTTCATTGCCTGATTCGACGCTTAACGCGTGTTATCTGGGCTTCGAGAGACATCGCTATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                   <  1:1062170/149‑1 (MQ=11)
            tGCCGCATCCGACATCAACGCCTGATGCGACGGTTAACGCGTCTTATAAGGCCTACGCCAGACAGCGCAAAAGCCTGATTTAGAGAGATTTTGTAGGTCGGATAAGCCGTTTACGCCGCATCCGACATCAATCCCTGATGCGACGCTTg                                                                                                                                   >  1:777348/1‑149 (MQ=11)
            tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTATGTCGCATACGGAGTTTACGCAGCATCCGACATCAATGCCTGATACGACGCTTg                                                                                                                                   >  1:897314/1‑149 (MQ=11)
            tGCCGCATCCGACATCAACGCCTGATCCGACGCTTAACGCGTCTTATCAGGCCTACGCCTGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                   <  1:1530758/149‑1 (MQ=255)
             gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTCATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGAGACGCATGc                                                                                                                                  >  1:931429/1‑149 (MQ=255)
              ccGCATTCGACTTCAACTCCTGTTGCGTCGCTTATCGCTTCTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                 <  1:1339528/149‑1 (MQ=11)
               cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGccc                                                                                                                                >  1:1035919/1‑148 (MQ=21)
                gcctCCGACATCAACGCCTGATGCGACGCTTTACGCTTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTATCGTTATTTTTTAGGGCGGATAAGGCGTTTTCGCCGCTTCAGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                               <  2:509496/146‑1 (MQ=11)
                   tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                            >  1:252302/1‑149 (MQ=25)
                    ccGACATCAACGCCTGATGCGACGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                                                                           <  2:1024422/149‑1 (MQ=255)
                     cGACCTCACCGCCTGATTCCACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCTCATGATGCGACGCTTGCCGCGTCtt                                                                                                                          <  2:900948/149‑1 (MQ=11)
                       tcttCAATGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATATCCTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                        <  2:1100117/146‑1 (MQ=11)
                        cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                       >  2:967512/1‑149 (MQ=25)
                        cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCATGATGCGACGCTTGCCGCGTCTTATc                                                                                                                       >  1:568545/1‑149 (MQ=25)
                         aTCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAAAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                      <  2:14475/149‑1 (MQ=255)
                         aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                      >  1:104456/1‑149 (MQ=25)
                         aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                      >  1:554187/1‑149 (MQ=25)
                         aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                      >  2:1467251/1‑149 (MQ=25)
                          tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                     <  1:1188317/149‑1 (MQ=37)
                           cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGACTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                    >  1:572566/1‑149 (MQ=25)
                           cAACGCCGGATCCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                    <  2:538134/149‑1 (MQ=11)
                            aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                   >  1:1369513/1‑149 (MQ=25)
                             aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGCCGTATACGCCGCATACGACTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCATGca                                                                                                                  >  1:1188932/1‑148 (MQ=11)
                             aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                  >  1:633104/1‑149 (MQ=25)
                               gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTACGCCTCATCCGACATCAACGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCATa                                                                                                                >  2:1345422/1‑149 (MQ=11)
                               gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCCACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTa                                                                                                                >  2:1339271/1‑149 (MQ=11)
                                ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                             >  1:1080841/1‑119 (MQ=32)
                                 cTGATGCGACGCTTAAAGCGTCTTATCATGACTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAACAGGCCTatc                                                                                                              <  1:1014754/149‑3 (MQ=11)
                                 cTGATAAGACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTagg                                                                                                              >  1:694618/1‑147 (MQ=11)
                                  tGATGCGACGCTTTACGAGTCTTATCAGGCCTACGCTAGACAGCGTTATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTATACGCCGGATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                                                                             <  1:1057671/149‑1 (MQ=11)
                                   gATGCGACGCTTTCCGCGTCTTATCAGGCGTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATATGGCGTTTACGCCGCTTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                                            <  2:1375261/149‑1 (MQ=11)
                                   gATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGGCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTGTACGCCGGATCCGACATCAATGCCTGATGCGACGGTTGCCGCGTCTGATCAGGCCTatctt                                                                                                            >  2:1527794/1‑145 (MQ=11)
                                    aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACggaa                                                                                                           >  2:725217/1‑146 (MQ=21)
                                      gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                    <  1:132289/122‑1 (MQ=17)
                                      gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                    >  2:132289/1‑122 (MQ=17)
                                        gACGCTTAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                       <  1:1382470/149‑1 (MQ=37)
                                                ccgcgTCTTATCTTGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                               <  2:444858/148‑1 (MQ=255)
                                                 cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                             >  2:333726/1‑102 (MQ=32)
                                                 cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                             <  1:333726/102‑1 (MQ=32)
                                                   ggTCTTATCATGCCTACGCCAGACATCGCAATAGCATGATTTAGCTTGATTTTGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                                            <  2:625200/148‑1 (MQ=11)
                                                      tttctCAGGCCTACGCCTGAGATCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCTACTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGa                                                                                         <  1:1352124/145‑1 (MQ=11)
                                                           gaggggtACGTCAGAGAGCTCAATTGCATTATTTAGCTTGTTTTTTTATGTCGGATCAGGCGTTTACGCCTCATCCGTCATCAAAGCCTGATGCGACGCTTGCAGCGTCTTATCAGGGCTACGCAAGACAGCGCAATAGCCTGATTTAg                                                                                    <  2:1175623/143‑1 (MQ=11)
                                                           ctggCCTAGGCCTGACTTCGGATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                                    <  1:268141/147‑1 (MQ=11)
                                                                       aGACTGCGAATTAGCCTGCTTAATCGTGAATTGGTAGTACTTATTAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                        <  2:1188932/149‑1 (MQ=11)
                                                                                     ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGAAAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                          >  1:1096568/1‑149 (MQ=255)
                                                                                                gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                          <  2:1131649/74‑1 (MQ=255)
                                                                                                gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                          >  1:1131649/1‑74 (MQ=255)
                                                                                                  gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                        <  2:1039536/74‑1 (MQ=255)
                                                                                                  gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                        >  1:1039536/1‑74 (MQ=255)
                                                                                                            gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGGCATcgcc                                   >  1:331109/1‑146 (MQ=255)
                                                                                                               ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                    <  2:1298191/113‑1 (MQ=39)
                                                                                                               ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                    >  1:1298191/1‑113 (MQ=39)
                                                                                                                 atcaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                              <  1:1099327/146‑1 (MQ=37)
                                                                                                                    aGGCGTTTACGCCGCATCCGACTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                           <  1:719457/149‑1 (MQ=37)
                                                                                                                    aGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCACGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                           <  1:1036631/149‑1 (MQ=255)
                                                                                                                     ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                              <  2:1136387/65‑3 (MQ=255)
                                                                                                                     ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                              >  1:1136387/1‑65 (MQ=255)
                                                                                                                              gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                    <  2:150392/98‑1 (MQ=37)
                                                                                                                              gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                 >  1:941224/1‑149 (MQ=34)
                                                                                                                                     ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTAAGCCGCTTCCGACCTCAACGCCTGATGCGACGCTTACcgggact          >  2:1431334/1‑144 (MQ=11)
                                                                                                                                      cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATGTAGCGTGATTTAGAAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCtt         >  1:1516771/1‑149 (MQ=11)
                                                                                                                                      cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCAAGCCAGCGCATTAGAGTTATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAAGCGAAGCTTTAATCGTCtt         >  2:645762/1‑149 (MQ=11)
                                                                                                                                       gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa        >  1:1104159/1‑149 (MQ=21)
                                                                                                                                           tCAACGCCTGATGCGACGCTTGCCGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg    <  1:725217/149‑1 (MQ=18)
                                                                                                                                            cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg   >  1:1521416/1‑149 (MQ=17)
                                                                                                                                             aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAGGc  >  1:1069758/1‑149 (MQ=17)
                                                                                                                                               |                                                                                                                                                  
ATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC  >  NC_000913/4295917‑4296206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: