Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,297,196 A→C 6.6% intergenic (‑71/+23) yjcO ← / ← fdhF Sel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,297,1960AC 6.6% 248.8 / 3.3 106intergenic (‑71/+23)yjcO/fdhFSel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base A (53/42);  new base C (3/6);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGC  >  NC_000913/4297050‑4297342
                                                                                                                                                  |                                                                                                                                                  
aCTGGCTGCCAGTCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAagg                                                                                                                                                  <  1:97765/149‑1 (MQ=255)
aCTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAagg                                                                                                                                                  <  2:1378766/149‑1 (MQ=255)
   ggCTGCCAGGCTCTGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAaggagg                                                                                                                                               <  1:358127/149‑1 (MQ=255)
    gCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATTCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGc                                                                                                                                              >  1:1345458/1‑149 (MQ=255)
    gCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGc                                                                                                                                              >  1:761674/1‑149 (MQ=255)
     cTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGTTAATTATTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAaggagggt                                                                                                                                             >  2:843339/1‑147 (MQ=255)
     cTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCt                                                                                                                                             <  1:206179/149‑1 (MQ=255)
       gCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTAAAAAACAACCTCAATGCTCTAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGt                                                                                                                                           <  2:405613/149‑1 (MQ=255)
        ccAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGtt                                                                                                                                          <  1:798610/149‑2 (MQ=255)
        ccAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTa                                                                                                                                          <  1:920870/149‑1 (MQ=255)
          aGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGtttt                                                                                                                                        <  2:439616/149‑4 (MQ=255)
            gCTCGGCGGCGTGGGCGGTGGCAAAGAATCTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGCTTGGGTAGCGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGaaa                                                                                                                                      <  2:843814/149‑1 (MQ=255)
            gCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGtttttt                                                                                                                                      <  1:818487/149‑6 (MQ=255)
               cGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGa                                                                                                                                   >  2:760950/1‑149 (MQ=255)
               cGGAGACGGTGGCGGGGGCAAAGAAAGTCAAAAACAACCTCATTGCGCTAACTATTATCAATTTGATCATACGATTGGTTAGGGAGTAACCAGTATAAAGAGTTCGAATGTATAAAAAAACAGCCTCCGACAGGAGGCTGTAGAAAGGa                                                                                                                                   <  2:1391514/149‑1 (MQ=255)
                 gAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACg                                                                                                                                 <  1:70261/149‑1 (MQ=255)
                  agacGTTGGCGTGGGCAAAGAATCTCAAAAACAACATCAATGCGATAATTTTTATCATTTTGATAAAACCATTGGGTACGGAGAAACCAGTAAAAATAGATCGAAAGGATAAAAAAACAGCCTCCGAAAGGAGGCTGAAAAAAGAACgg                                                                                                                                >  2:744816/4‑149 (MQ=255)
                  aGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACgg                                                                                                                                <  2:664996/149‑1 (MQ=255)
                   gTCGTTGGCTTGGGCAAATAATGTCAAAAACACCATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGt                                                                                                                               <  1:1456612/149‑1 (MQ=255)
                     ggTTGGCGTGGGCAAAGAATGTCAAAATCAACATCAATGCGATTATTTTTTTCATTTTGATTATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAt                                                                                                                             <  1:797637/148‑1 (MQ=255)
                       ttGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTa                                                                                                                           >  1:137170/1‑149 (MQ=255)
                       ttGGCCTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCAATTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGACGCTGTAGAAAGGACGGTATTa                                                                                                                           >  2:88264/1‑149 (MQ=255)
                            gTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCa                                                                                                                      >  2:40532/1‑149 (MQ=255)
                                gCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGc                                                                                                                  >  2:1091409/1‑149 (MQ=255)
                                    aGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACATCATCAGAAAGGATGCTGTAGAAAGTACGGTATTACTCCAGTGCCgat                                                                                                              >  1:1016193/1‑147 (MQ=255)
                                          tCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCgcgc                                                                                                        >  1:808998/1‑149 (MQ=255)
                                          tCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGACGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCgcgc                                                                                                        >  1:1000389/1‑149 (MQ=255)
                                            aaaaaCAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAg                                                                                                      >  1:496246/1‑149 (MQ=255)
                                            aaaaaCAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAg                                                                                                      >  1:1381955/1‑149 (MQ=255)
                                            aaaaaCAACATCAATGCGAAAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAg                                                                                                      <  1:1381579/149‑1 (MQ=255)
                                                acaacaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGa                                                                                                  >  1:551111/1‑149 (MQ=255)
                                                     aTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGtttt                                                                                             <  2:37426/149‑1 (MQ=255)
                                                      tCAATGCGATAATTTTTTTCATTTTGATAATACGAATGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAACGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTc                                                                                            <  1:1246269/149‑1 (MQ=255)
                                                        aaTGCGATAATTTTTTTCATTTTGATAATACGATAGGGTACGGAGGAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCaa                                                                                          <  1:891585/149‑1 (MQ=255)
                                                            cGATAATTTTTTTCATTTTGATTATACGATTGGGGCGGGAGTAACCAGTATACAGAGTTCGAATTGTTAAAAAATCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGACAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttg                                                                                      <  1:119560/149‑1 (MQ=255)
                                                            cGATAATTTTTATCATTTTGAAAATCCGATTGGGAAGGGAGTACCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAACGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttg                                                                                      <  1:1340950/149‑1 (MQ=255)
                                                              aTAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGACTTCGAATGTATAATACAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgtt                                                                                    <  2:501292/149‑1 (MQ=255)
                                                              aTAATTTTTTTCATTTTGATAAAACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgtt                                                                                    <  2:570723/149‑1 (MQ=255)
                                                               aaaTTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGAATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgttg                                                                                   <  1:911809/148‑1 (MQ=255)
                                                                 aTTTTTTTCATTTTGATAATACGATTGGGTAGGGACTAACCAGTATAAAGAGTTCGAATGGATTAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTa                                                                                 <  1:689537/149‑1 (MQ=255)
                                                                  tttttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGCCGGTATTACGCCAGTGCCGCTTCGCGCAGGCGGGTTTTCAACTTGTAGTAc                                                                                >  2:881222/1‑149 (MQ=255)
                                                                          aTTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCAGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAAt                                                                        <  2:1182777/149‑1 (MQ=255)
                                                                             ttGATAATACGATTGGGTAGGTAGTAACCAGAATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGGCAATCAc                                                                     <  1:296957/149‑1 (MQ=255)
                                                                                attattcGATTGGGTAGGTAGTAAGCAGTATAAAGAGTGGGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACTGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTAAACTTGTTGTACTCGTCAATCACGTa                                                                  <  1:1176015/143‑1 (MQ=255)
                                                                                aaaataCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTa                                                                  <  2:248358/147‑1 (MQ=255)
                                                                                 taataCGATTGGGTAGGGAGTAACCAGAATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTAc                                                                 <  2:727125/149‑1 (MQ=255)
                                                                                 aaataCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGTATAAACAAACAGCATCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTTTACTCGTCAATCACGTAc                                                                 <  2:732919/148‑1 (MQ=255)
                                                                                    taCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGc                                                              >  1:493297/1‑149 (MQ=255)
                                                                                       gATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcg                                                           >  1:494905/1‑149 (MQ=255)
                                                                                       gATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGACTTTTCAACTTGTTGTACACGTCAATCACTTACTGCTcg                                                           >  2:1458754/1‑149 (MQ=255)
                                                                                             gTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGgcggcg                                                     >  1:613816/1‑149 (MQ=255)
                                                                                             gTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGgcggcg                                                     >  1:1057555/1‑149 (MQ=255)
                                                                                             gTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGgcggcg                                                     >  2:463978/1‑149 (MQ=255)
                                                                                              tAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAATGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGgcgc                                                    >  1:1060589/1‑149 (MQ=255)
                                                                                              tAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGgcgc                                                    <  2:780851/149‑1 (MQ=255)
                                                                                              tAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGgcgc                                                    <  1:529219/149‑1 (MQ=255)
                                                                                                    gTAACCAGTATAAAGAGTTCGATTGGATAAAAAAACAGCCTCCGAAAGGCGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGGCAATCACGTACTGCTCGGCGGCGCGCTgat                                              >  1:1011230/1‑149 (MQ=255)
                                                                                                       aCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                           >  2:1303755/1‑149 (MQ=255)
                                                                                                       aCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                           <  1:682570/149‑1 (MQ=255)
                                                                                                         cAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcg                                         >  1:433571/1‑149 (MQ=255)
                                                                                                         cAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTATCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGAGCGCTgatctgcg                                         >  1:232292/1‑149 (MQ=255)
                                                                                                           gTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgat                                       >  1:1552113/1‑149 (MQ=255)
                                                                                                            tataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGCCGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAGCGTGTTGTACTCGTCATTCACGTACTGCTCGGCGGCGCGCTggacggcgatc                                      >  2:868933/1‑149 (MQ=255)
                                                                                                            tataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                      >  1:1503982/1‑149 (MQ=255)
                                                                                                            tataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                      <  2:621650/149‑1 (MQ=255)
                                                                                                            gaaaaaGAGTTCCAATGGAGAAAAACACAGCCTACGAAAGGAGGCTGTAGAAAGGACGTTATTTCGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCTACTTGTTGTCCTGGTCAATCACGTACTGCTGGGCGGCGCGCTgatcggcgatc                                      <  2:1041495/146‑1 (MQ=255)
                                                                                                              taAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACTGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcgg                                    >  1:1020709/1‑149 (MQ=255)
                                                                                                              aaaaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcgg                                    <  1:436712/148‑1 (MQ=255)
                                                                                                               aaaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcggc                                   >  2:765088/1‑149 (MQ=255)
                                                                                                               aaaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcggc                                   <  1:760950/149‑1 (MQ=255)
                                                                                                                aaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCt                                  <  2:551111/149‑1 (MQ=255)
                                                                                                                 agagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTc                                 >  1:1168533/1‑149 (MQ=255)
                                                                                                                 agagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTc                                 >  1:1521303/1‑149 (MQ=255)
                                                                                                                 agagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTc                                 >  1:9626/1‑149 (MQ=255)
                                                                                                                  gagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCg                                >  1:609516/1‑149 (MQ=255)
                                                                                                                  gagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCg                                <  2:638419/149‑1 (MQ=255)
                                                                                                                        gAATGGATAAAAAAACAGCCTCCGAAAGGCGGCTGTAGAAAGGACCGTATTCCGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGa                          <  1:518772/149‑1 (MQ=255)
                                                                                                                         aaTGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCTGCGGCTCGCTGATCGGCGATCGGCTCGAcgcgca                         >  2:878624/1‑147 (MQ=255)
                                                                                                                             gATAAAAAAACAGCCTCCGAATGGAGGCTGTAGATAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgc                     >  1:141327/1‑149 (MQ=255)
                                                                                                                              aTAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTCCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGCCGCGAACGgcg                    >  1:1054835/1‑149 (MQ=255)
                                                                                                                               tAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCACGGCGGCGCGCTGATCGGCGATAGGCTCGACGCGAACGgcgc                   >  2:361981/1‑149 (MQ=255)
                                                                                                                               tAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACATGTTGTACTCGTCAATCACGTACTGCTCAGCGGCGCGCTGCTCGGCGATCGGCTCGACGCGAACGgcgc                   >  1:930707/1‑149 (MQ=255)
                                                                                                                               tAAAAAAACAGCCACCGAACGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTGGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcgc                   <  2:1551301/149‑1 (MQ=255)
                                                                                                                                aaaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGCCGCGACCGGCGCa                  >  1:371160/1‑149 (MQ=255)
                                                                                                                                aaaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCAGCGCTCAGGCGAGTTTTCAACTAGTTGTACTCGACAAGCAGGTACTGATCGGCGGCGGGCTGGTCGGCGAGAGGGTCGACGCGAAAGgcgct                  >  2:920367/1‑148 (MQ=255)
                                                                                                                                 tttctaCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGCTCGGCGATCGGCTCGACGCGAACGGCGCAg                 >  1:1482987/6‑149 (MQ=255)
                                                                                                                                  aaaaaCAGCCTCCGAAATGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGt                >  2:1024935/1‑149 (MQ=255)
                                                                                                                                  aaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGt                >  1:188108/1‑149 (MQ=255)
                                                                                                                                     taCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAtt             >  1:1211703/2‑149 (MQ=255)
                                                                                                                                     taCAGCCTCCTGTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGGTTGCAACTTGTTGTACTCGTCATTCACGTCCTGCTCGGCGGCGCGCTGAGCGGCGTTCGGCTCGACGCGATCGCCGCAGGAtt             >  1:1315540/2‑149 (MQ=255)
                                                                                                                                      aCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAttt            <  2:761674/149‑1 (MQ=255)
                                                                                                                                      aCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAttt            <  1:687482/149‑1 (MQ=255)
                                                                                                                                         gCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTa         >  1:1553470/1‑149 (MQ=255)
                                                                                                                                          ccTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACTTTCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTAc        <  1:1458754/149‑1 (MQ=255)
                                                                                                                                          ccTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGATGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTAc        >  2:581394/1‑149 (MQ=255)
                                                                                                                                             ccGAAAGGAGGCTCTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTACAACTTGATGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATAGGCTCGACGCGACCCCCGCAGTATTTGTACTcc     >  1:1570558/1‑149 (MQ=255)
                                                                                                                                              cGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCGCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCg    <  1:1091409/149‑1 (MQ=255)
                                                                                                                                                aaaGGAGGCTGTATAAAGGACTGTATTACGCTAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGc  <  2:1345458/149‑1 (MQ=255)
                                                                                                                                                aaaGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGc  <  2:778801/149‑1 (MQ=255)
                                                                                                                                                aaaGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCATCGCGCAGGGGAGTTTTCAACTTGATGTACTCGTCCATCACGTACAGCTCGGCGGCGCGCTGCTCGGCGATCGCCTCGACGCGAACGGCGCACTATATGTACACCgcc  >  2:122448/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGC  >  NC_000913/4297050‑4297342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: