Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,985 T→A 5.2% intergenic (‑90/+21) phnL ← / ← phnK ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,9850TA 5.2% 316.9 / 4.4 112intergenic (‑90/+21)phnL/phnKribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (40/64);  new base A (2/4);  total (42/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCA  >  NC_000913/4317837‑4318130
                                                                                                                                                    |                                                                                                                                                 
cgcacgcaGTTTTGCTGGTGCAGGATGAAGGGTTAACTGACGTTTTGTTCGTTAATCATTTTTCGCCTCGTGGTTCGGGGGGCGGATGCGACGCGGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGttt                                                                                                                                                   <  2:842202/149‑1 (MQ=37)
cgcACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGttt                                                                                                                                                   <  2:96173/149‑1 (MQ=255)
    cGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTActc                                                                                                                                               >  1:260135/1‑147 (MQ=255)
     gCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                              >  2:1467511/1‑149 (MQ=255)
     gCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAAACATTTTTCGCGTCGTGGTTCGGGGGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                              <  2:1468181/149‑1 (MQ=255)
       cGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCGTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGAGTTTAcgccgc                                                                                                                                            >  2:477200/1‑149 (MQ=255)
       cGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                            >  1:557680/1‑149 (MQ=255)
          tttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                         <  1:233525/149‑6 (MQ=38)
           ttGCTGGTGCAGGATGAAGGTTTTACTGACGTGTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACTCCGCATcc                                                                                                                                        <  1:549328/149‑1 (MQ=255)
            tGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                       <  1:209172/149‑1 (MQ=255)
            tGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTAAATCATTTTACGCCTCGTGGTTGGGGTGTCGGATGCGACGCTGACGCGTATTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                       <  2:106551/149‑1 (MQ=255)
            tGCTGGTGCAGGATGAAGGTTTTACTGACGTGTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACTGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                       <  1:215469/149‑1 (MQ=255)
             gCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                      >  1:1311984/1‑149 (MQ=255)
              cgggggCAGGATTAAGGGTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     <  1:931708/144‑1 (MQ=25)
              cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                     >  2:624228/1‑149 (MQ=255)
                 gTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAcc                                                                                                                                  >  1:631153/1‑149 (MQ=255)
                   gCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCgg                                                                                                                                <  2:1274582/149‑1 (MQ=255)
                     aGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCt                                                                                                                              >  2:864823/1‑149 (MQ=255)
                        aTGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCaa                                                                                                                           <  2:1293573/149‑1 (MQ=255)
                        aTGAAGGGTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCaa                                                                                                                           <  2:19670/149‑1 (MQ=255)
                          gAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAtt                                                                                                                         <  2:118720/149‑1 (MQ=255)
                            ctctgttACTGCCGTTTTGTACCTTACTCATTTTTCGCCTCTTGGGCCGGGTGTCGCATGCGACGCGGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCACTTCt                                                                                                                       <  2:1082728/144‑1 (MQ=21)
                             ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTGCGGGTGTCGGAGGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                      <  2:1172304/149‑1 (MQ=255)
                             ggTTTTACTGACGTCTAGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTAGGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                      <  2:800841/149‑1 (MQ=255)
                                  tACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGGGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCaaaa                                                                                                                 <  2:476241/149‑1 (MQ=255)
                                    cTGACGTTTTGTACGTTAATCTTTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAATCC                                                                                                               >  2:1340782‑M1/1‑149 (MQ=255)
                                     tGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCG                                                                                                              >  1:864384‑M1/1‑149 (MQ=255)
                                      gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGA                                                                                                             >  2:1282514‑M1/1‑149 (MQ=255)
                                       ccGTTTTGTCCGTTAATCATTTTTCGCCTCGTGGTTCGGGGGTCGGAGGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                            <  1:103442/148‑1 (MQ=255)
                                       aCGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTGCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                            <  2:1151637/149‑1 (MQ=255)
                                         gTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGAGGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGa                                                                                                          <  1:276725/149‑1 (MQ=255)
                                         gTTTTGTACGTTAATCATTTTTAGCCTCGTCCTTCGGGTGTAGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCTGCACCGGCTCAATTCTGCAAAACCCATGa                                                                                                          >  1:1206166/1‑149 (MQ=25)
                                          ttttGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGac                                                                                                         <  1:1409708/149‑1 (MQ=255)
                                            ttGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGacac                                                                                                       <  1:404325/149‑1 (MQ=255)
                                            ttGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGacac                                                                                                       <  2:614535/149‑1 (MQ=255)
                                                     aaTCATTTTTCGCCTCGTGGTGCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCGCAATTCTGCAAAACCGATGACACCAGCAGCtg                                                                                              <  2:101903/149‑1 (MQ=255)
                                                      aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgt                                                                                             <  1:1362910/149‑1 (MQ=255)
                                                      aTCATTTTTCGCCTCGTGGGTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCTGATAAGGCTTTTACGCCGCATCCGGCACAGGCTCAATTCTTCAAAACCGATGACACCAGCAGCtgt                                                                                             <  2:1269406/149‑1 (MQ=25)
                                                      aTCATTTTTCGCATCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgt                                                                                             <  2:382585/149‑1 (MQ=255)
                                                       tCATTTTTCGCCTCGGGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCATAACCGATGACACCAGCAGCtgtg                                                                                            <  1:1290582/149‑1 (MQ=255)
                                                        gttttttCGCCTCGTGGTTCGGGTGTCGGATGCGTCGGTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGGATCCGGCTGCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTgtgt                                                                                           <  1:624228/147‑1 (MQ=37)
                                                          tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGtat                                                                                         >  1:398038/1‑149 (MQ=255)
                                                            tttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATAc                                                                                       <  2:557680/149‑1 (MQ=255)
                                                                     gTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGc                                                                              >  2:1335851/1‑149 (MQ=255)
                                                                     gTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGc                                                                              >  1:716445/1‑149 (MQ=255)
                                                                      tgtatCGGGTGTCGGATGCTACGCTGACGCGTCTTATCTGACCTACGGGTAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCACCGGCTCAATTCTGCAATACCGATGACACCAGCAGCTGTGTATACGGATGATGCg                                                                             <  2:1206166/145‑1 (MQ=37)
                                                                      tGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCg                                                                             <  2:398038/149‑1 (MQ=255)
                                                                        gTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                           <  1:213912/149‑1 (MQ=255)
                                                                         ttCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGgt                                                                          >  2:1136231/1‑149 (MQ=255)
                                                                          tCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGgtc                                                                         <  2:647496/149‑1 (MQ=255)
                                                                            gggTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAATACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGt                                                                       <  1:446708‑M2/149‑2 (MQ=255)
                                                                            gggTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGt                                                                       <  1:1033235‑M2/149‑2 (MQ=255)
                                                                            gggTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCGGTGTATACGGATGATGCGGGTCGt                                                                       <  1:1082509‑M2/149‑2 (MQ=255)
                                                                            gggTGTCGGATGCGACGCTGACGCGTCTTATCCGACCGACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGt                                                                       >  2:59093‑M2/1‑148 (MQ=255)
                                                                              gtgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGCGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCTCCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATTGGGAGGATGCGGGTCGtcg                                                                     <  1:512072‑M2/149‑4 (MQ=255)
                                                                              gtgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGtcg                                                                     <  2:124182‑M2/149‑4 (MQ=255)
                                                                               tgtCGGTTGCGACGCGGACGCTGATTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCACCGGCTCAATTCTGCAAAACCGAcgacccgcatcatccgtatacacagctgctggtgtcatcgg                                                                    <  2:401602‑M1/149‑42 (MQ=255)
                                                                               tgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGa                                                                    <  1:167745/149‑1 (MQ=255)
                                                                                   ggATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCGCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAc                                                                >  2:1149564/1‑149 (MQ=255)
                                                                                     aTGCGACGCTGACGCGGCTTATCCGACCTACGGGGAGCGCAGTTGTAGGCCGGATAAGGCGTTGACGCCGCATCCGGCACCGGCTCACTTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAcgc                                                              <  1:1135802/149‑1 (MQ=255)
                                                                                         gACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAcgacccgcatcatccgtatacacagctgctggtgtcat                                                                       <  1:773670‑M1/136‑39 (MQ=255)
                                                                                         gACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAcgacccgcatcatccgtatacacagctgctggtgtcat                                                                       >  2:773670‑M1/1‑98 (MQ=255)
                                                                                           cGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcgg                                                        <  2:957617/149‑1 (MQ=255)
                                                                                            gCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcggt                                                       <  1:647607/149‑1 (MQ=255)
                                                                                            ccTGACGCGTCTTAACCGACCTACGGGGAGCGCATATGTACGCCGGATCAGGCGGTCACGCCGCATCCGGCACCGGCGCAATTCTGCAAAACCGAGGACACCAGCAGCTGGGTATACGGATGATGCGGGTCGTCGAGCACGcggtcggt                                                       <  2:1033971/148‑1 (MQ=255)
                                                                                                     tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                              >  1:218425/1‑149 (MQ=255)
                                                                                                     tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                              <  2:260135/149‑1 (MQ=255)
                                                                                                     tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGAAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGACGGTTAACCCAct                                              >  1:1426840/1‑149 (MQ=255)
                                                                                                      cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCActc                                             <  2:572621/149‑1 (MQ=255)
                                                                                                         aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                          >  1:1197441/1‑149 (MQ=255)
                                                                                                         aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                          >  1:759388/1‑149 (MQ=255)
                                                                                                         aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCGCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                          <  1:357905/149‑1 (MQ=255)
                                                                                                           ccGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCGGTGTATACGGATGCTGCGGGTCGTCGAGCCCGCGGTCGGTTAACGCACTCTcccc                                        >  1:1004266/1‑147 (MQ=255)
                                                                                                            cGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTccacc                                       >  1:725736/1‑149 (MQ=255)
                                                                                                                 tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGGGTATACGGATGATGCGGGTCGTCGAGCCCGCGGTCGGTTAACCCACTCGCCAGCACTTg                                  >  2:268816/1‑149 (MQ=255)
                                                                                                                  aCGGGGAGCGCATTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCACCGGCTAAATTCTGCAAAACCGCTGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGGCGGTTAACCCACTCTCCACCACTTGc                                 <  2:814209/149‑1 (MQ=255)
                                                                                                                  aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                                 >  1:1204224/1‑149 (MQ=255)
                                                                                                                  aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                                 <  1:1467511/149‑1 (MQ=255)
                                                                                                                    ggggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGccc                               <  2:1311984/149‑1 (MQ=255)
                                                                                                                     gggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCGACTCTCCACCACTTGcccc                              >  2:589132/1‑149 (MQ=255)
                                                                                                                      ggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                             >  2:985440/1‑149 (MQ=255)
                                                                                                                         gcgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCt                          >  2:1396821/1‑149 (MQ=255)
                                                                                                                          cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCtt                         <  1:985440/149‑1 (MQ=255)
                                                                                                                           gcATTTGTCGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAACACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                        <  2:495016/149‑1 (MQ=255)
                                                                                                                             ttttGTAGTCCGGATAAGGCGTTTACGCCGCATCCGGCACCTGCTCAATTCTGCAAAACCGTTGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTAGAGCACGCGGTCGGTTAACCCTGTCTCCACCACTTGCCCCTGCTtcat                      <  2:551334/148‑1 (MQ=39)
                                                                                                                             aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                      >  1:709506/1‑149 (MQ=255)
                                                                                                                              tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatc                     <  1:883375/149‑1 (MQ=255)
                                                                                                                              tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatc                     <  1:821846/149‑1 (MQ=255)
                                                                                                                                tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAc                   <  1:826072/149‑1 (MQ=255)
                                                                                                                                 gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAcc                  >  2:1088748/1‑149 (MQ=255)
                                                                                                                                   aGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAGTTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGAGGATGCGGGTCGTCGCGCACGCGGTCGGTTAGCCCCCTCTCCACCACTTGCCCCTGCGTCATCACCAg                >  2:822353/1‑149 (MQ=255)
                                                                                                                                       ctgATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAACCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaaa            <  1:864823/147‑1 (MQ=255)
                                                                                                                                          aTAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCCCTCTCCACCACTTGCCCCTGCTGCAGCACGCGCAAACgg         >  2:1154941/1‑149 (MQ=255)
                                                                                                                                             aGGCGTTTACGCCGCATCCTGCACCTGCTCAATTCTGCAAAACCTTTGACAGCAGAAGATGTGTATACGGATGATGCTGTTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCAGCACTTGCCCCTGCTTCATCACCAGCAAACGGccc      <  2:630170/149‑1 (MQ=255)
                                                                                                                                             aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTcc      <  2:265470/149‑1 (MQ=255)
                                                                                                                                              ggCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAACCCGATGACACCCGCCGGTGTGGATACAGATGATGCGGGTCGTCGAGCACGCGGTAGGGTAACCCACTCTCCACAACTTGCCCCTGCTTGATCCACAGCAACCGGTCCg     >  2:413991/1‑149 (MQ=255)
                                                                                                                                               gCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAACCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACACACTCTCCACCCCTTGCCCCTGCGTCCTCACCAGCAACCGGTCCGc    >  2:964626/1‑149 (MQ=255)
                                                                                                                                               gCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGc    >  2:816917/1‑149 (MQ=255)
                                                                                                                                                 gTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACCCCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCca  <  1:1282514/149‑1 (MQ=255)
                                                                                                                                                 gTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCca  <  2:864384/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCA  >  NC_000913/4317837‑4318130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: