Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,058 G→A 7.6% intergenic (‑317/+341) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0580GA 7.6% 197.8 / 7.3 79intergenic (‑317/+341)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base A (3/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTT  >  NC_000913/4325910‑4326201
                                                                                                                                                    |                                                                                                                                               
gATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATg                                                                                                                                                 <  1:608603/149‑1 (MQ=37)
    cgACGCTCAAGCGCCGCACCCGGCAGCCGCCACCACTGCCGGATGCGGCGTAACCCCCGTATCCGGCCTACGGAGCGTGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggc                                                                                                                                             <  2:644961/148‑1 (MQ=11)
         cgcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTaaa                                                                                                                                        <  1:1194119/149‑1 (MQ=37)
          gcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAAc                                                                                                                                       >  1:469451/1‑149 (MQ=37)
             aGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                    <  2:47455/149‑1 (MQ=37)
                   gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                              <  2:989897/149‑1 (MQ=11)
                   gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                              <  2:353542/149‑1 (MQ=11)
                   gCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                              >  1:944891/1‑149 (MQ=37)
                     aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                            <  1:10186/149‑1 (MQ=17)
                      tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                           >  1:1244124/1‑149 (MQ=37)
                      tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                           <  1:250515/149‑1 (MQ=37)
                        cggCAGTCGGCACAATTGCCGGATGGGGCGTAAACGCCTTATCCGGCCTACGGAGGTTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                                                                                                                         <  2:31583/148‑3 (MQ=11)
                        aGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                         >  2:431281/1‑149 (MQ=37)
                         ggCAGTCGGCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                        <  2:12844/149‑1 (MQ=17)
                         ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                        <  1:1447819/149‑1 (MQ=17)
                          gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                       >  2:819429/1‑149 (MQ=37)
                              tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                   <  2:1375611/149‑1 (MQ=37)
                                ggCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                                                                                                 <  2:1177620/149‑1 (MQ=17)
                                ggCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg                                                                                                                 >  2:1139852/1‑149 (MQ=17)
                                 gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGt                                                                                                                <  2:469451/149‑1 (MQ=37)
                                   aCCATTGCCGGAAGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCTCATCAGGCAGAGGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAtgctgc                                                                                                              >  2:26107/1‑149 (MQ=11)
                                    ctgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                             <  1:283659/146‑1 (MQ=1)
                                    ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                             <  1:435673/149‑1 (MQ=34)
                                    ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATTTGTACGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                             <  1:838313/149‑1 (MQ=21)
                                     cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                            <  1:208076/147‑1 (MQ=14)
                                     cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                            <  1:823901/147‑1 (MQ=14)
                                      aTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg                                                                                                           >  1:997735/1‑149 (MQ=32)
                                        ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCAGGCCTAAGGGTGGGGCgggta                                                                                                         >  2:551267/2‑147 (MQ=11)
                                           cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                      <  2:1244124/149‑1 (MQ=32)
                                            ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTg                                                                                                     <  2:959015/149‑1 (MQ=32)
                                             gATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                    <  2:1121481/149‑1 (MQ=32)
                                               tGCGGCGTAAACGGCTTATGCGGCCTACGTAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                  <  2:81050/149‑1 (MQ=255)
                                                gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGGAAAACCCGTATACGGCCGACGGGTGGGGGGGGGAGTTGTAgg                                                                                                 >  1:1525443/1‑149 (MQ=11)
                                                   gcgTAAACGCCTTATCCGGCCTACGTCGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                              <  2:812506/149‑1 (MQ=12)
                                                      tAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                           <  2:1251743/149‑1 (MQ=17)
                                                              ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAcgcg                                                                                   >  1:898102/1‑149 (MQ=32)
                                                                      ctttcGGAGGGGGGGGGATTTAGTATGCCTGATACGACGCGAAAGCGTCGGATCAGGCAGTCGTCACCGTTGGCGGATGCGTCGTTAACGCCTTATCCGGACATCGGATGCTGCGGGTTTTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                           <  2:1549735/145‑1 (MQ=2)
                                                                          cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                       >  1:493711/1‑149 (MQ=32)
                                                                              gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATAAGGCATACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGGCGCATCAgg                                                                   >  2:1096237/1‑149 (MQ=11)
                                                                              gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                   <  2:973892/149‑1 (MQ=32)
                                                                                  gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGt                                                               <  2:241908/149‑1 (MQ=33)
                                                                                   cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                              <  1:1487999/149‑1 (MQ=34)
                                                                                     ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGAGTAAACGCCTTATACGGCCTACGGATGGAGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGGTgg                                                            >  2:819443/1‑149 (MQ=11)
                                                                                       aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                          <  2:650538/149‑1 (MQ=37)
                                                                                            gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                              >  2:1316407/1‑108 (MQ=32)
                                                                                              aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                                   >  2:86053/1‑149 (MQ=39)
                                                                                               ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                                  <  2:997735/149‑1 (MQ=39)
                                                                                                 ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCgg                                                >  2:1009362/1‑149 (MQ=21)
                                                                                                  cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                               >  2:850451/1‑149 (MQ=39)
                                                                                                    gATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                    <  1:671118/94‑1 (MQ=18)
                                                                                                     aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgc                                            >  2:1342229/1‑149 (MQ=39)
                                                                                                        aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                            >  2:14495/1‑82 (MQ=33)
                                                                                                         gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggcg                                        <  1:1421747/149‑1 (MQ=39)
                                                                                                            gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTAATCCGGCCAAAGGCTGGTGACGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTAGCATCAGGCAGAGGGCACTGTTGCCGGATGCGGGGtca                                     >  1:1084445/1‑147 (MQ=11)
                                                                                                              gcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAAc                                   >  1:1260242/1‑149 (MQ=39)
                                                                                                                aaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGCTGCGGCGTAAACGc                                 >  1:1511379/1‑149 (MQ=255)
                                                                                                                         aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCGGATAAGAAGCGCAAGCGTCGCATCAGTCAGATGGCAGAGTTGCCGGATGCGGCGAAATCGCCTTATCCgg                        >  1:379958/1‑149 (MQ=11)
                                                                                                                          tCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGc                       >  2:728327/1‑149 (MQ=255)
                                                                                                                            aGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                     >  2:767607/1‑149 (MQ=255)
                                                                                                                             ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACTCCTTATACGGCCTACGGAAGGTGCTGGAAATTTTAGGACTGATAAGACGCCCAAGCGTCGAATAAAGCAGTAGGCACTGTTGCCGGGTGCGGCGGACACGCCGTAGACGGCGTa                    >  2:1052742/1‑149 (MQ=11)
                                                                                                                             ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTa                    >  2:1447907/1‑149 (MQ=255)
                                                                                                                               cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                  <  1:1342229/149‑1 (MQ=39)
                                                                                                                               cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcagacg                  >  1:338329/1‑144 (MQ=255)
                                                                                                                                  tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                               >  2:1115833/1‑69 (MQ=35)
                                                                                                                                  tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg               <  1:900566/149‑1 (MQ=40)
                                                                                                                                   gggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg              <  1:1139852/148‑1 (MQ=39)
                                                                                                                                   gggCACCGTTGCCGGATGCGGCGTAAACGCCTTAGCAGGCCTTCGGTTTGTTCGGTAATTTGTAGGACTGATAAGACGCGCAAGCGTCGCATCAGGCGGTTGGCACTGTTGCCGGATGCGGCGTAAACGACTTATCCGGCCTACGGAgg              <  2:996432/148‑1 (MQ=14)
                                                                                                                                   cGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                  >  1:1463070/1‑65 (MQ=35)
                                                                                                                                      cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg           >  2:549400/1‑149 (MQ=39)
                                                                                                                                      cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg           <  1:850451/149‑1 (MQ=39)
                                                                                                                                      cACCGTTGCCGGATGCGGCGTAAACGCCTTATACGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCAGTTGGAACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTg           >  2:1269077/1‑149 (MQ=255)
                                                                                                                                        ccGTGGCCGGAGGCGGCGTAACCGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg         <  2:829242/149‑1 (MQ=255)
                                                                                                                                         cGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg        >  1:1531306/1‑149 (MQ=38)
                                                                                                                                           ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                           <  1:178019/64‑1 (MQ=35)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt    >  2:449609/1‑149 (MQ=11)
                                                                                                                                              ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                       >  2:1491663/1‑81 (MQ=33)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt   >  1:1072770/1‑149 (MQ=11)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt   >  1:623057/1‑149 (MQ=11)
                                                                                                                                               cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCGTACGGAGGGGGAGGGAAttt  >  1:1029646/1‑149 (MQ=255)
                                                                                                                                               cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                               >  1:1152470/1‑104 (MQ=17)
                                                                                                                                               cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                               <  2:1152470/104‑1 (MQ=17)
                                                                                                                                                    |                                                                                                                                               
GATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTT  >  NC_000913/4325910‑4326201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: