Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,059 C→A 6.7% intergenic (‑318/+340) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0590CA 6.7% 183.1 / 2.3 79intergenic (‑318/+340)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base A (3/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGC  >  NC_000913/4325914‑4326207
                                                                                                                                                 |                                                                                                                                                    
cgACGCTCAAGCGCCGCACCCGGCAGCCGCCACCACTGCCGGATGCGGCGTAACCCCCGTATCCGGCCTACGGAGCGTGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggc                                                                                                                                                   <  2:644961/148‑1 (MQ=11)
     cgcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTaaa                                                                                                                                              <  1:1194119/149‑1 (MQ=37)
      gcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAAc                                                                                                                                             >  1:469451/1‑149 (MQ=37)
         aGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                          <  2:47455/149‑1 (MQ=37)
               gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                    <  2:989897/149‑1 (MQ=11)
               gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                    <  2:353542/149‑1 (MQ=11)
               gCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                    >  1:944891/1‑149 (MQ=37)
                 aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                  <  1:10186/149‑1 (MQ=17)
                  tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                 >  1:1244124/1‑149 (MQ=37)
                  tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                 <  1:250515/149‑1 (MQ=37)
                    cggCAGTCGGCACAATTGCCGGATGGGGCGTAAACGCCTTATCCGGCCTACGGAGGTTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                                                                                                                               <  2:31583/148‑3 (MQ=11)
                    aGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                               >  2:431281/1‑149 (MQ=37)
                     ggCAGTCGGCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                              <  2:12844/149‑1 (MQ=17)
                     ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                              <  1:1447819/149‑1 (MQ=17)
                      gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                             >  2:819429/1‑149 (MQ=37)
                          tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                         <  2:1375611/149‑1 (MQ=37)
                            ggCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                                                                                                       <  2:1177620/149‑1 (MQ=17)
                            ggCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg                                                                                                                       >  2:1139852/1‑149 (MQ=17)
                             gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGt                                                                                                                      <  2:469451/149‑1 (MQ=37)
                               aCCATTGCCGGAAGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCTCATCAGGCAGAGGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAtgctgc                                                                                                                    >  2:26107/1‑149 (MQ=11)
                                ctgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                   <  1:283659/146‑1 (MQ=1)
                                ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                   <  1:435673/149‑1 (MQ=34)
                                ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATTTGTACGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                   <  1:838313/149‑1 (MQ=21)
                                 cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                  <  1:208076/147‑1 (MQ=14)
                                 cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                  <  1:823901/147‑1 (MQ=14)
                                  aTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg                                                                                                                 >  1:997735/1‑149 (MQ=32)
                                    ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCAGGCCTAAGGGTGGGGCgggta                                                                                                               >  2:551267/2‑147 (MQ=11)
                                       cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                            <  2:1244124/149‑1 (MQ=32)
                                        ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTg                                                                                                           <  2:959015/149‑1 (MQ=32)
                                         gATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                          <  2:1121481/149‑1 (MQ=32)
                                           tGCGGCGTAAACGGCTTATGCGGCCTACGTAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                        <  2:81050/149‑1 (MQ=255)
                                            gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGGAAAACCCGTATACGGCCGACGGGTGGGGGGGGGAGTTGTAgg                                                                                                       >  1:1525443/1‑149 (MQ=11)
                                               gcgTAAACGCCTTATCCGGCCTACGTCGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                    <  2:812506/149‑1 (MQ=12)
                                                  tAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                 <  2:1251743/149‑1 (MQ=17)
                                                          ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAcgcg                                                                                         >  1:898102/1‑149 (MQ=32)
                                                                  ctttcGGAGGGGGGGGGATTTAGTATGCCTGATACGACGCGAAAGCGTCGGATCAGGCAGTCGTCACCGTTGGCGGATGCGTCGTTAACGCCTTATCCGGACATCGGATGCTGCGGGTTTTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                                 <  2:1549735/145‑1 (MQ=2)
                                                                      cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                             >  1:493711/1‑149 (MQ=32)
                                                                          gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATAAGGCATACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGGCGCATCAgg                                                                         >  2:1096237/1‑149 (MQ=11)
                                                                          gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                         <  2:973892/149‑1 (MQ=32)
                                                                              gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGt                                                                     <  2:241908/149‑1 (MQ=33)
                                                                               cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                                    <  1:1487999/149‑1 (MQ=34)
                                                                                 ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGAGTAAACGCCTTATACGGCCTACGGATGGAGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGGTgg                                                                  >  2:819443/1‑149 (MQ=11)
                                                                                   aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                <  2:650538/149‑1 (MQ=37)
                                                                                        gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                    >  2:1316407/1‑108 (MQ=32)
                                                                                          aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                                         >  2:86053/1‑149 (MQ=39)
                                                                                           ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                                        <  2:997735/149‑1 (MQ=39)
                                                                                             ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCgg                                                      >  2:1009362/1‑149 (MQ=21)
                                                                                              cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                     >  2:850451/1‑149 (MQ=39)
                                                                                                gATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                          <  1:671118/94‑1 (MQ=18)
                                                                                                 aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgc                                                  >  2:1342229/1‑149 (MQ=39)
                                                                                                    aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                  >  2:14495/1‑82 (MQ=33)
                                                                                                     gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggcg                                              <  1:1421747/149‑1 (MQ=39)
                                                                                                        gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTAATCCGGCCAAAGGCTGGTGACGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTAGCATCAGGCAGAGGGCACTGTTGCCGGATGCGGGGtca                                           >  1:1084445/1‑147 (MQ=11)
                                                                                                          gcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAAc                                         >  1:1260242/1‑149 (MQ=39)
                                                                                                            aaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGCTGCGGCGTAAACGc                                       >  1:1511379/1‑149 (MQ=255)
                                                                                                                     aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCGGATAAGAAGCGCAAGCGTCGCATCAGTCAGATGGCAGAGTTGCCGGATGCGGCGAAATCGCCTTATCCgg                              >  1:379958/1‑149 (MQ=11)
                                                                                                                      tCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGc                             >  2:728327/1‑149 (MQ=255)
                                                                                                                        aGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                           >  2:767607/1‑149 (MQ=255)
                                                                                                                         ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACTCCTTATACGGCCTACGGAAGGTGCTGGAAATTTTAGGACTGATAAGACGCCCAAGCGTCGAATAAAGCAGTAGGCACTGTTGCCGGGTGCGGCGGACACGCCGTAGACGGCGTa                          >  2:1052742/1‑149 (MQ=11)
                                                                                                                         ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTa                          >  2:1447907/1‑149 (MQ=255)
                                                                                                                           cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                        <  1:1342229/149‑1 (MQ=39)
                                                                                                                           cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcagacg                        >  1:338329/1‑144 (MQ=255)
                                                                                                                              tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                                     >  2:1115833/1‑69 (MQ=35)
                                                                                                                              tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                     <  1:900566/149‑1 (MQ=40)
                                                                                                                               gggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg                    <  1:1139852/148‑1 (MQ=39)
                                                                                                                               gggCACCGTTGCCGGATGCGGCGTAAACGCCTTAGCAGGCCTTCGGTTTGTTCGGTAATTTGTAGGACTGATAAGACGCGCAAGCGTCGCATCAGGCGGTTGGCACTGTTGCCGGATGCGGCGTAAACGACTTATCCGGCCTACGGAgg                    <  2:996432/148‑1 (MQ=14)
                                                                                                                               cGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                        >  1:1463070/1‑65 (MQ=35)
                                                                                                                                  cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                 >  2:549400/1‑149 (MQ=39)
                                                                                                                                  cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                 <  1:850451/149‑1 (MQ=39)
                                                                                                                                  cACCGTTGCCGGATGCGGCGTAAACGCCTTATACGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCAGTTGGAACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTg                 >  2:1269077/1‑149 (MQ=255)
                                                                                                                                    ccGTGGCCGGAGGCGGCGTAACCGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg               <  2:829242/149‑1 (MQ=255)
                                                                                                                                     cGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg              >  1:1531306/1‑149 (MQ=38)
                                                                                                                                       ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                 <  1:178019/64‑1 (MQ=35)
                                                                                                                                         gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt          >  2:449609/1‑149 (MQ=11)
                                                                                                                                          ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                             >  2:1491663/1‑81 (MQ=33)
                                                                                                                                          ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt         >  1:1072770/1‑149 (MQ=11)
                                                                                                                                          ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt         >  1:623057/1‑149 (MQ=11)
                                                                                                                                           cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCGTACGGAGGGGGAGGGAAttt        >  1:1029646/1‑149 (MQ=255)
                                                                                                                                           cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                     >  1:1152470/1‑104 (MQ=17)
                                                                                                                                           cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                     <  2:1152470/104‑1 (MQ=17)
                                                                                                                                                 cggcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc  <  1:86053/149‑1 (MQ=37)
                                                                                                                                                 cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc  <  1:577671/149‑1 (MQ=17)
                                                                                                                                                 cggcgTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCGGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc  <  1:1096237/149‑1 (MQ=12)
                                                                                                                                                 |                                                                                                                                                    
AGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGC  >  NC_000913/4325914‑4326207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: