Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,065 A→T 8.8% intergenic (‑324/+334) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0650AT 8.8% 194.6 / 7.0 84intergenic (‑324/+334)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base A (38/38);  new base T (4/4);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGA  >  NC_000913/4325919‑4326211
                                                                                                                                                  |                                                                                                                                                  
cgcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTaaa                                                                                                                                                  <  1:1194119/149‑1 (MQ=37)
 gcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAAc                                                                                                                                                 >  1:469451/1‑149 (MQ=37)
    aGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                              <  2:47455/149‑1 (MQ=37)
          gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                        <  2:989897/149‑1 (MQ=11)
          gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                        <  2:353542/149‑1 (MQ=11)
          gCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                        >  1:944891/1‑149 (MQ=37)
            aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                      <  1:10186/149‑1 (MQ=17)
             tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     >  1:1244124/1‑149 (MQ=37)
             tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     <  1:250515/149‑1 (MQ=37)
               cggCAGTCGGCACAATTGCCGGATGGGGCGTAAACGCCTTATCCGGCCTACGGAGGTTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                                                                                                                                   <  2:31583/148‑3 (MQ=11)
               aGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                                   >  2:431281/1‑149 (MQ=37)
                ggCAGTCGGCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                                  <  2:12844/149‑1 (MQ=17)
                ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                                  <  1:1447819/149‑1 (MQ=17)
                 gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                 >  2:819429/1‑149 (MQ=37)
                     tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                             <  2:1375611/149‑1 (MQ=37)
                       ggCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                                                                                                           <  2:1177620/149‑1 (MQ=17)
                       ggCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg                                                                                                                           >  2:1139852/1‑149 (MQ=17)
                        gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGt                                                                                                                          <  2:469451/149‑1 (MQ=37)
                          aCCATTGCCGGAAGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCTCATCAGGCAGAGGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAtgctgc                                                                                                                        >  2:26107/1‑149 (MQ=11)
                           ctgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                       <  1:283659/146‑1 (MQ=1)
                           ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                       <  1:435673/149‑1 (MQ=34)
                           ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATTTGTACGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                       <  1:838313/149‑1 (MQ=21)
                            cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                      <  1:208076/147‑1 (MQ=14)
                            cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                      <  1:823901/147‑1 (MQ=14)
                             aTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg                                                                                                                     >  1:997735/1‑149 (MQ=32)
                               ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCAGGCCTAAGGGTGGGGCgggta                                                                                                                   >  2:551267/2‑147 (MQ=11)
                                  cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                                <  2:1244124/149‑1 (MQ=32)
                                   ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTg                                                                                                               <  2:959015/149‑1 (MQ=32)
                                    gATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                              <  2:1121481/149‑1 (MQ=32)
                                      tGCGGCGTAAACGGCTTATGCGGCCTACGTAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                            <  2:81050/149‑1 (MQ=255)
                                       gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGGAAAACCCGTATACGGCCGACGGGTGGGGGGGGGAGTTGTAgg                                                                                                           >  1:1525443/1‑149 (MQ=11)
                                          gcgTAAACGCCTTATCCGGCCTACGTCGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                        <  2:812506/149‑1 (MQ=12)
                                             tAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                     <  2:1251743/149‑1 (MQ=17)
                                                     ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAcgcg                                                                                             >  1:898102/1‑149 (MQ=32)
                                                             ctttcGGAGGGGGGGGGATTTAGTATGCCTGATACGACGCGAAAGCGTCGGATCAGGCAGTCGTCACCGTTGGCGGATGCGTCGTTAACGCCTTATCCGGACATCGGATGCTGCGGGTTTTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                                     <  2:1549735/145‑1 (MQ=2)
                                                                 cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                                 >  1:493711/1‑149 (MQ=32)
                                                                     gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATAAGGCATACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGGCGCATCAgg                                                                             >  2:1096237/1‑149 (MQ=11)
                                                                     gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                             <  2:973892/149‑1 (MQ=32)
                                                                         gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGt                                                                         <  2:241908/149‑1 (MQ=33)
                                                                          cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                                        <  1:1487999/149‑1 (MQ=34)
                                                                            ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGAGTAAACGCCTTATACGGCCTACGGATGGAGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGGTgg                                                                      >  2:819443/1‑149 (MQ=11)
                                                                              aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                    <  2:650538/149‑1 (MQ=37)
                                                                                   gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                        >  2:1316407/1‑108 (MQ=32)
                                                                                     aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                                             >  2:86053/1‑149 (MQ=39)
                                                                                      ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                                            <  2:997735/149‑1 (MQ=39)
                                                                                        ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCgg                                                          >  2:1009362/1‑149 (MQ=21)
                                                                                         cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                         >  2:850451/1‑149 (MQ=39)
                                                                                           gATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                              <  1:671118/94‑1 (MQ=18)
                                                                                            aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgc                                                      >  2:1342229/1‑149 (MQ=39)
                                                                                               aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                      >  2:14495/1‑82 (MQ=33)
                                                                                                gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggcg                                                  <  1:1421747/149‑1 (MQ=39)
                                                                                                   gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTAATCCGGCCAAAGGCTGGTGACGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTAGCATCAGGCAGAGGGCACTGTTGCCGGATGCGGGGtca                                               >  1:1084445/1‑147 (MQ=11)
                                                                                                     gcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAAc                                             >  1:1260242/1‑149 (MQ=39)
                                                                                                       aaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGCTGCGGCGTAAACGc                                           >  1:1511379/1‑149 (MQ=255)
                                                                                                                aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCGGATAAGAAGCGCAAGCGTCGCATCAGTCAGATGGCAGAGTTGCCGGATGCGGCGAAATCGCCTTATCCgg                                  >  1:379958/1‑149 (MQ=11)
                                                                                                                 tCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGc                                 >  2:728327/1‑149 (MQ=255)
                                                                                                                   aGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                               >  2:767607/1‑149 (MQ=255)
                                                                                                                    ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACTCCTTATACGGCCTACGGAAGGTGCTGGAAATTTTAGGACTGATAAGACGCCCAAGCGTCGAATAAAGCAGTAGGCACTGTTGCCGGGTGCGGCGGACACGCCGTAGACGGCGTa                              >  2:1052742/1‑149 (MQ=11)
                                                                                                                    ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTa                              >  2:1447907/1‑149 (MQ=255)
                                                                                                                      cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                            <  1:1342229/149‑1 (MQ=39)
                                                                                                                      cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcagacg                            >  1:338329/1‑144 (MQ=255)
                                                                                                                         tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                                         >  2:1115833/1‑69 (MQ=35)
                                                                                                                         tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                         <  1:900566/149‑1 (MQ=40)
                                                                                                                          gggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg                        <  1:1139852/148‑1 (MQ=39)
                                                                                                                          gggCACCGTTGCCGGATGCGGCGTAAACGCCTTAGCAGGCCTTCGGTTTGTTCGGTAATTTGTAGGACTGATAAGACGCGCAAGCGTCGCATCAGGCGGTTGGCACTGTTGCCGGATGCGGCGTAAACGACTTATCCGGCCTACGGAgg                        <  2:996432/148‑1 (MQ=14)
                                                                                                                          cGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                            >  1:1463070/1‑65 (MQ=35)
                                                                                                                             cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                     >  2:549400/1‑149 (MQ=39)
                                                                                                                             cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                     <  1:850451/149‑1 (MQ=39)
                                                                                                                             cACCGTTGCCGGATGCGGCGTAAACGCCTTATACGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCAGTTGGAACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTg                     >  2:1269077/1‑149 (MQ=255)
                                                                                                                               ccGTGGCCGGAGGCGGCGTAACCGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg                   <  2:829242/149‑1 (MQ=255)
                                                                                                                                cGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg                  >  1:1531306/1‑149 (MQ=38)
                                                                                                                                  ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                     <  1:178019/64‑1 (MQ=35)
                                                                                                                                    gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt              >  2:449609/1‑149 (MQ=11)
                                                                                                                                     ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                                 >  2:1491663/1‑81 (MQ=33)
                                                                                                                                     ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt             >  1:1072770/1‑149 (MQ=11)
                                                                                                                                     ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt             >  1:623057/1‑149 (MQ=11)
                                                                                                                                      cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCGTACGGAGGGGGAGGGAAttt            >  1:1029646/1‑149 (MQ=255)
                                                                                                                                      cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                         >  1:1152470/1‑104 (MQ=17)
                                                                                                                                      cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                         <  2:1152470/104‑1 (MQ=17)
                                                                                                                                            cggcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc      <  1:86053/149‑1 (MQ=37)
                                                                                                                                            cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc      <  1:577671/149‑1 (MQ=17)
                                                                                                                                            cggcgTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCGGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc      <  1:1096237/149‑1 (MQ=12)
                                                                                                                                                gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                                        <  2:996345/79‑1 (MQ=35)
                                                                                                                                                gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGAGTAAACGCCTTAGACGGCATACGGAGGGTGCGGGAATTTGTAGGCCTGa  >  2:487952/1‑149 (MQ=11)
                                                                                                                                                gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCGGATGAGGAGTAAACGCCTTATCCGTCCTAAGGAGTGTGCGGGAATTTGTAGGCCTGa  >  2:718386/1‑149 (MQ=11)
                                                                                                                                                  |                                                                                                                                                  
CGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGA  >  NC_000913/4325919‑4326211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: