Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,072 T→G 7.6% intergenic (‑331/+327) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0720TG 7.6% 183.4 / 4.8 83intergenic (‑331/+327)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base G (4/3);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAG  >  NC_000913/4325929‑4326215
                                                                                                                                               |                                                                                                                                               
gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                            <  2:989897/149‑1 (MQ=11)
gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                            <  2:353542/149‑1 (MQ=11)
gCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                            >  1:944891/1‑149 (MQ=37)
  aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                          <  1:10186/149‑1 (MQ=17)
   tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                         >  1:1244124/1‑149 (MQ=37)
   tCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                         <  1:250515/149‑1 (MQ=37)
     cggCAGTCGGCACAATTGCCGGATGGGGCGTAAACGCCTTATCCGGCCTACGGAGGTTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                                                                                                                                       <  2:31583/148‑3 (MQ=11)
     aGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                                       >  2:431281/1‑149 (MQ=37)
      ggCAGTCGGCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                                      <  2:12844/149‑1 (MQ=17)
      ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                                      <  1:1447819/149‑1 (MQ=17)
       gCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                     >  2:819429/1‑149 (MQ=37)
           tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                                 <  2:1375611/149‑1 (MQ=37)
             ggCACTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                                                                                                               <  2:1177620/149‑1 (MQ=17)
             ggCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg                                                                                                                               >  2:1139852/1‑149 (MQ=17)
              gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGt                                                                                                                              <  2:469451/149‑1 (MQ=37)
                aCCATTGCCGGAAGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCTCATCAGGCAGAGGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAtgctgc                                                                                                                            >  2:26107/1‑149 (MQ=11)
                 ctgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                           <  1:283659/146‑1 (MQ=1)
                 ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                           <  1:435673/149‑1 (MQ=34)
                 ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATTTGTACGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCg                                                                                                                           <  1:838313/149‑1 (MQ=21)
                  cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                          <  1:208076/147‑1 (MQ=14)
                  cgttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                          <  1:823901/147‑1 (MQ=14)
                   aTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg                                                                                                                         >  1:997735/1‑149 (MQ=32)
                     ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCAGGCCTAAGGGTGGGGCgggta                                                                                                                       >  2:551267/2‑147 (MQ=11)
                        cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                                    <  2:1244124/149‑1 (MQ=32)
                         ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTg                                                                                                                   <  2:959015/149‑1 (MQ=32)
                          gATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                                  <  2:1121481/149‑1 (MQ=32)
                            tGCGGCGTAAACGGCTTATGCGGCCTACGTAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                                <  2:81050/149‑1 (MQ=255)
                             gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGGAAAACCCGTATACGGCCGACGGGTGGGGGGGGGAGTTGTAgg                                                                                                               >  1:1525443/1‑149 (MQ=11)
                                gcgTAAACGCCTTATCCGGCCTACGTCGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                            <  2:812506/149‑1 (MQ=12)
                                   tAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                         <  2:1251743/149‑1 (MQ=17)
                                           ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAcgcg                                                                                                 >  1:898102/1‑149 (MQ=32)
                                                   ctttcGGAGGGGGGGGGATTTAGTATGCCTGATACGACGCGAAAGCGTCGGATCAGGCAGTCGTCACCGTTGGCGGATGCGTCGTTAACGCCTTATCCGGACATCGGATGCTGCGGGTTTTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                                         <  2:1549735/145‑1 (MQ=2)
                                                       cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                                     >  1:493711/1‑149 (MQ=32)
                                                           gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATAAGGCATACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGGCGCATCAgg                                                                                 >  2:1096237/1‑149 (MQ=11)
                                                           gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                                 <  2:973892/149‑1 (MQ=32)
                                                               gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGt                                                                             <  2:241908/149‑1 (MQ=33)
                                                                cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                                            <  1:1487999/149‑1 (MQ=34)
                                                                  ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGAGTAAACGCCTTATACGGCCTACGGATGGAGCGGGAATTAGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGGTgg                                                                          >  2:819443/1‑149 (MQ=11)
                                                                    aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                        <  2:650538/149‑1 (MQ=37)
                                                                         gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                            >  2:1316407/1‑108 (MQ=32)
                                                                           aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                                                 >  2:86053/1‑149 (MQ=39)
                                                                            ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                                                <  2:997735/149‑1 (MQ=39)
                                                                              ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCgg                                                              >  2:1009362/1‑149 (MQ=21)
                                                                               cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                             >  2:850451/1‑149 (MQ=39)
                                                                                 gATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                                  <  1:671118/94‑1 (MQ=18)
                                                                                  aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgc                                                          >  2:1342229/1‑149 (MQ=39)
                                                                                     aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                          >  2:14495/1‑82 (MQ=33)
                                                                                      gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggcg                                                      <  1:1421747/149‑1 (MQ=39)
                                                                                         gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTAATCCGGCCAAAGGCTGGTGACGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTAGCATCAGGCAGAGGGCACTGTTGCCGGATGCGGGGtca                                                   >  1:1084445/1‑147 (MQ=11)
                                                                                           gcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAAc                                                 >  1:1260242/1‑149 (MQ=39)
                                                                                             aaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGCTGCGGCGTAAACGc                                               >  1:1511379/1‑149 (MQ=255)
                                                                                                      aTCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCGGATAAGAAGCGCAAGCGTCGCATCAGTCAGATGGCAGAGTTGCCGGATGCGGCGAAATCGCCTTATCCgg                                      >  1:379958/1‑149 (MQ=11)
                                                                                                       tCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGc                                     >  2:728327/1‑149 (MQ=255)
                                                                                                         aGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                   >  2:767607/1‑149 (MQ=255)
                                                                                                          ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACTCCTTATACGGCCTACGGAAGGTGCTGGAAATTTTAGGACTGATAAGACGCCCAAGCGTCGAATAAAGCAGTAGGCACTGTTGCCGGGTGCGGCGGACACGCCGTAGACGGCGTa                                  >  2:1052742/1‑149 (MQ=11)
                                                                                                          ggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTa                                  >  2:1447907/1‑149 (MQ=255)
                                                                                                            cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                <  1:1342229/149‑1 (MQ=39)
                                                                                                            cAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcagacg                                >  1:338329/1‑144 (MQ=255)
                                                                                                               tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                                             >  2:1115833/1‑69 (MQ=35)
                                                                                                               tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                             <  1:900566/149‑1 (MQ=40)
                                                                                                                gggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg                            <  1:1139852/148‑1 (MQ=39)
                                                                                                                gggCACCGTTGCCGGATGCGGCGTAAACGCCTTAGCAGGCCTTCGGTTTGTTCGGTAATTTGTAGGACTGATAAGACGCGCAAGCGTCGCATCAGGCGGTTGGCACTGTTGCCGGATGCGGCGTAAACGACTTATCCGGCCTACGGAgg                            <  2:996432/148‑1 (MQ=14)
                                                                                                                cGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                                >  1:1463070/1‑65 (MQ=35)
                                                                                                                   cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                         <  1:850451/149‑1 (MQ=39)
                                                                                                                   cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                         >  2:549400/1‑149 (MQ=39)
                                                                                                                   cACCGTTGCCGGATGCGGCGTAAACGCCTTATACGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCAGTTGGAACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTg                         >  2:1269077/1‑149 (MQ=255)
                                                                                                                     ccGTGGCCGGAGGCGGCGTAACCGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg                       <  2:829242/149‑1 (MQ=255)
                                                                                                                      cGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg                      >  1:1531306/1‑149 (MQ=38)
                                                                                                                        ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                         <  1:178019/64‑1 (MQ=35)
                                                                                                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt                  >  2:449609/1‑149 (MQ=11)
                                                                                                                           ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt                                                                                     >  2:1491663/1‑81 (MQ=33)
                                                                                                                           ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt                 >  1:1072770/1‑149 (MQ=11)
                                                                                                                           ccGGATAAGGCGTTTACGCCGCATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTCGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt                 >  1:623057/1‑149 (MQ=11)
                                                                                                                            cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCGTACGGAGGGGGAGGGAAttt                >  1:1029646/1‑149 (MQ=255)
                                                                                                                            cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                             <  2:1152470/104‑1 (MQ=17)
                                                                                                                            cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                             >  1:1152470/1‑104 (MQ=17)
                                                                                                                                  cggcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc          <  1:86053/149‑1 (MQ=37)
                                                                                                                                  cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc          <  1:577671/149‑1 (MQ=17)
                                                                                                                                  cggcgTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCGGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc          <  1:1096237/149‑1 (MQ=12)
                                                                                                                                      gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGtt                                                                            <  2:996345/79‑1 (MQ=35)
                                                                                                                                      gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGAGTAAACGCCTTAGACGGCATACGGAGGGTGCGGGAATTTGTAGGCCTGa      >  2:487952/1‑149 (MQ=11)
                                                                                                                                      gTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCGGATGAGGAGTAAACGCCTTATCCGTCCTAAGGAGTGTGCGGGAATTTGTAGGCCTGa      >  2:718386/1‑149 (MQ=11)
                                                                                                                                          aCGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAg  >  2:37649/1‑149 (MQ=17)
                                                                                                                                               |                                                                                                                                               
GCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAG  >  NC_000913/4325929‑4326215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: