BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,339,802	A→G	5.4%	A240A (GCT→GCC)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,802	0	A	G	5.4%	307.1 / 6.9	112	A240A (GCT→GCC)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (57/47); new base G (4/2); total (62/50)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01

Only 100 of 113 total aligned reads displayed.

TGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATG  >  NC_000913/4339659‑4339949

tGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTg                                                                                                                                                >  2:220952/1‑149 (MQ=255)

 tcGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTgg                                                                                                                                               >  1:396603/2‑148 (MQ=255)

 gCGGATAGATTGGCCCGTTTATGGGGTAGCTGTTGCGGGTTGGCACCATATAGACGGGTTTCGCGCCTGTCAGCTTGAAAGGTTGTTCGATGGTTTTATGGCAGTTAGGGTCAACGTCCTCGACATGGTTATCGGTCATGCAAGCCTGc                                                                                                                                               <  1:963277/149‑1 (MQ=255)

  cGGATTGATTGGCCCGATAATGCCGTAGCGGTTGCGGATTGGCACCATATAGAGCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCa                                                                                                                                              <  1:215783/149‑1 (MQ=255)

  cGGATAGATTGGCCCGATACTGCCGTAGAGTTTGCGGCTTGGCAACATATAGACCGGTTTCGCGTCTGTCAGCATCAATCCTTGTTCGATGGATTTTTGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCa                                                                                                                                              <  1:1020384/149‑1 (MQ=255)

     aTAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAGGCTTGCatga                                                                                                                                           <  2:975975/149‑1 (MQ=255)

      ttgATTGGCCCGATACTGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCatgat                                                                                                                                          <  1:399331/147‑1 (MQ=255)

         aTTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGGCATGCAGGCTTGCatgaccga                                                                                                                                       <  1:498748/149‑5 (MQ=255)

            ggCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGCCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCg                                                                                                                                    >  1:583899/1‑149 (MQ=255)

             gCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCgg                                                                                                                                   >  2:41735/1‑149 (MQ=255)

             gCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCgg                                                                                                                                   >  2:1467763/1‑149 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCACAGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                  >  1:148848/1‑149 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                  <  1:10469/149‑1 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGGCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                  <  1:1440737/149‑1 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGGTTCGCGCCTGTCCGCTTCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGCCCACGCCATCGTTATCGGACATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                  >  1:1015240/1‑149 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACAGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGCCCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                  >  1:1019132/1‑149 (MQ=255)

              cccGATAATGCCGTAGCGGTTGCGGATTGGCACCATATAGACCGGTATAGCGCCTGTCAGCATCAATCCTTGTTCGATGGATTTGTGGCAGTTACGGACAACGACCACGACATCATTATCGGTCATGCAAGGCTGCATGATGGTGCGGt                                                                                                                                  >  1:368167/1‑149 (MQ=255)

                cGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTa                                                                                                                                <  1:823617/149‑1 (MQ=255)

                  aTAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTaga                                                                                                                              <  2:253217/149‑1 (MQ=255)

                   tAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCTTTATAGGTCATGCAAGCCTGCATGATGGTGCGGTTagag                                                                                                                             <  2:584408/149‑1 (MQ=255)

                     aTGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGcc                                                                                                                           <  1:78178/149‑1 (MQ=255)

                        ccGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGa                                                                                                                        >  2:1099666/1‑149 (MQ=255)

                        ccGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCGTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGGTAGAGCCGGa                                                                                                                        >  1:1539168/1‑149 (MQ=255)

                         cGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGaa                                                                                                                       <  1:1297494/149‑1 (MQ=255)

                         cGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGaa                                                                                                                       <  2:952564/149‑1 (MQ=255)

                         cGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGACAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGaa                                                                                                                       <  2:627050/149‑1 (MQ=255)

                           tAGCGGTTGCGGCTGGGCACCATATAGACCGGCTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGGCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGt                                                                                                                     <  1:1106824/149‑1 (MQ=255)

                             gCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTAc                                                                                                                   <  2:1324206/149‑1 (MQ=255)

                                 ttGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACcgac                                                                                                               <  1:46106/149‑1 (MQ=255)

                                     ggCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGCCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACt                                                                                                           <  2:583899/149‑1 (MQ=255)

                                      ggttGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTa                                                                                                          <  2:430151/147‑1 (MQ=255)

                                      gCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCCACGACTa                                                                                                          >  1:717223/1‑149 (MQ=255)

                                       cTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTAc                                                                                                         <  1:1554808/149‑1 (MQ=255)

                                        ttGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTAcc                                                                                                        >  2:977453/1‑149 (MQ=255)

                                               cATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGTTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAgg                                                                                                 >  2:1240233/1‑149 (MQ=255)

                                                atatAGCCCGGTTTCGCGCCTCTCAGCATCAAACCTTGTTCGAAGGATTTATGGGAGTTACGGTCAACGACCACGACTTCGTTATCGGTCATTCTAGCCTGCATGATGGTGCGGTTAGAGCAGGAAGTACCGACGACTACCgtcacgca                                                                                                >  1:864335/1‑142 (MQ=255)

                                                  ataGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGCTGGTGCGGTTAGAGCCGTAAGTACCGACGACTTCCGACCAGGAGc                                                                                              >  2:705601/1‑149 (MQ=255)

                                                   taGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCg                                                                                             <  2:396603/149‑1 (MQ=255)

                                                     gACCGGTTTCGCGCCTGTCAGCATCACACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGCCCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGTCGACTACCGACCTGGAGCGAt                                                                                           >  2:321889/1‑149 (MQ=255)

                                                     gACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACTACATCGTAATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGgagagaa                                                                                           >  1:1394522/1‑148 (MQ=255)

                                                     gACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGGTATCGGTCATGCAAGCCGGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGCGCGAt                                                                                           >  2:441208/1‑149 (MQ=255)

                                                        cGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCgg                                                                                        <  2:385118/149‑1 (MQ=255)

                                                           tttCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCAGAAGTACCGACGACTACCGACCAGGAGCGATCGGCAc                                                                                     >  1:43039/1‑149 (MQ=255)

                                                           tttCGCGCCTGGCAGCATCAAACCTTGTTCGATGGATTTATCGCAGGTCCGGTCAACGACCACGACATCGTAATCGGTCATGCCAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCAc                                                                                     <  1:1546845/149‑1 (MQ=255)

                                                           gtgcgcgCCGGTCAGCATCAAACCTTGTTCGAGGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGGCAGGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCAc                                                                                     <  2:1157398/146‑1 (MQ=255)

                                                             tCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTAATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGTATGTACCGACGGCTACCGACCTGGAGCGATCGGCAcga                                                                                   >  2:1558878/1‑147 (MQ=255)

                                                              cgcgCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCaa                                                                                  <  1:1224703/149‑1 (MQ=255)

                                                               gcgcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGATGACTACCGACCAGGAGCGATCGGCACCaaa                                                                                 >  2:1005951/1‑149 (MQ=255)

                                                               gcgcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCaaa                                                                                 >  1:272035/1‑149 (MQ=255)

                                                               gcgcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCaaa                                                                                 >  1:133169/1‑149 (MQ=255)

                                                                cgcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGCCGACTACCGCCCAGGGGCGATCGGCACCAAAt                                                                                >  2:645770/1‑149 (MQ=255)

                                                                  ccTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACAAGGAGCGATCGGCACCAAATAc                                                                              >  1:931603/1‑149 (MQ=255)

                                                                      tCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGt                                                                          <  1:1554888/149‑1 (MQ=255)

                                                                            tCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCa                                                                    >  2:110869/1‑149 (MQ=255)

                                                                              aaaCCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCata                                                                  <  1:772415/149‑1 (MQ=255)

                                                                              aaaCCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCata                                                                  <  2:526541/149‑1 (MQ=255)

                                                                               aaCCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGTGATCGGCACCAAATACGCGTGCGGCatat                                                                 >  2:1288919/1‑149 (MQ=255)

                                                                               aaCCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCatat                                                                 >  1:1557224/1‑149 (MQ=255)

                                                                                  cgtGTTCGATGGATTTATGGCCGTTACGGTCAACGACCACGACATCGTTATCGGGCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACGAAATAGGCGTGCGGCATAtttt                                                              <  2:673265/147‑1 (MQ=255)

                                                                                   ttGTTCGATGGATTAATGGCCGTTACGGGCACCGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGGTAGAGCCGGAAGTACAGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATAttttt                                                             <  2:730727/149‑1 (MQ=255)

                                                                                         gATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTTCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCCTATTtttcgcttt                                                       >  1:1141065/1‑149 (MQ=255)

                                                                                         gATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgcttt                                                       >  2:591367/1‑149 (MQ=255)

                                                                                           tGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctttcg                                                     >  2:987652/1‑149 (MQ=255)

                                                                                           tGGATTTATGGCAGTTACGGTCAACGAACACGACATCGTTAACGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGAATACAGACCAGGTGCGATCGGCACCAAATACTCGTGCGGCATATTttacgctttcg                                                     >  2:1494990/1‑149 (MQ=255)

                                                                                               tttATGGCAGTTACGGTCAACGCCCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGCACAGGAGCGATCGGCACCAAATACGCGTGCGGAATATTATTCGCTTTCGCCaa                                                 >  1:1387786/1‑149 (MQ=255)

                                                                                                ttATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCaaa                                                >  2:611767/1‑149 (MQ=255)

                                                                                                  aTGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATg                                              >  1:1209781/1‑149 (MQ=255)

                                                                                                    ggCAGTTATGGTCACCGACCACGACATCGTTATCGGACATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATgcg                                            >  1:565653/1‑149 (MQ=255)

                                                                                                     gCAGTTACGGTCAACGACCACGACATCGTTATCGGGCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATgcgc                                           <  1:510951/149‑1 (MQ=255)

                                                                                                        gTTACGGTCAACGACCACGACATCGTGATCGGTCATGGAAGCCTGCATGATGCTGCGGAGAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATCCGCGTGCGGCATTTTTTTCGCGTACGCCACAGGCGCCAg                                        >  2:97362/1‑149 (MQ=255)

                                                                                                             ggTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTACgg                                   >  1:16955/1‑149 (MQ=255)

                                                                                                               tCACCGACCACGACTTCGTTATCGGTCATGCAAGGCTGGCTTATGGTGCGGTTAGAGCCGGAACTAGCGACGACTACCGACCAGTAGCGATGGTCACGAAATACGCGTGCGTCATATTTTTCTCTTTCTGCAAATGCGCCAGTATGGTc                                 <  2:368167/149‑1 (MQ=255)

                                                                                                               tCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGCCGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTc                                 >  2:255775/1‑149 (MQ=255)

                                                                                                               tCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTc                                 >  2:578950/1‑149 (MQ=255)

                                                                                                                cAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTcc                                >  1:739120/1‑148 (MQ=255)

                                                                                                                 aacgacaacgacATCTTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCTGGATCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCaa                               <  2:132341/149‑1 (MQ=255)

                                                                                                                 aaCGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTCGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCaa                               <  2:717223/149‑1 (MQ=255)

                                                                                                                       cACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACAGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTAGCTTTCGCCAAATGCGCCAGTAAGGTCAAGCAAAg                         >  2:329470/1‑149 (MQ=255)

                                                                                                                         cGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGaa                       <  1:1066108/149‑1 (MQ=255)

                                                                                                                            cATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCg                    >  1:59165/1‑149 (MQ=255)

                                                                                                                            cATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCg                    <  1:987652/149‑1 (MQ=255)

                                                                                                                             aTCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTGAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGa                   <  2:88592/149‑1 (MQ=255)

                                                                                                                              tCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAg                  <  1:444508/149‑1 (MQ=255)

                                                                                                                              tCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAg                  <  1:220952/149‑1 (MQ=255)

                                                                                                                                  tagcGGGCAAGCAAGACTGCATGATGGGGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGTAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGaa              <  2:1019132/146‑1 (MQ=255)

                                                                                                                                   caccatCATGCAGGCTTGCATGATGGTGCGGTTTGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAg             >  1:225811/6‑149 (MQ=255)

                                                                                                                                     cGGTCATGCACGCCTGCAGGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGACATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgtt           <  1:934242/149‑1 (MQ=255)

                                                                                                                                      catCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGCCGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGCACCGAGGGAAgttc          >  2:245075/3‑149 (MQ=255)

                                                                                                                                      catCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGCCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCCAGCAAAGACCCGAGGGAAggtc          >  1:1065722/3‑149 (MQ=255)

                                                                                                                                      catCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGCTCGGCCCCCACTACGCGTGCGGCATAGTTTTCGCTTTCGCCACATGAGACAGTATGGTCAAGCAAAGAACCGAGGGAAtttc          >  2:622501/3‑149 (MQ=255)

                                                                                                                                       gTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGCCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTACGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttcg         >  1:1264056/1‑149 (MQ=255)

                                                                                                                                       gTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttcg         >  1:1185315/1‑149 (MQ=255)

                                                                                                                                          aTGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttcgttc      <  2:148848/149‑1 (MQ=255)

                                                                                                                                           tGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTATCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACAGAGGGAAGttcgttcg     >  1:1174498/1‑149 (MQ=255)

                                                                                                                                           tGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCAGATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGttcgttcg     >  1:1062491/1‑149 (MQ=255)

                                                                                                                                            gCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGTCACCAAATACGCGTGCGGCATATTTTTGGCTTACGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGa    >  1:915798/1‑149 (MQ=255)

                                                                                                                                            gCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGGACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTAAAGCAACGAACCGAGGGATGTTCGTTCGa    >  2:1258206/1‑149 (MQ=255)

                                                                                                                                             cAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTTGTTCGAt   <  2:445800/149‑1 (MQ=255)

                                                                                                                                             cAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGAt   >  2:912755/1‑149 (MQ=255)

                                                                                                                                              aaGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGCCGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATg  >  2:580384/1‑149 (MQ=255)

TGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATG  >  NC_000913/4339659‑4339949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`