Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,958 G→T 5.7% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9580GT 5.7% 257.1 / 3.4 106intergenic (‑45/+28)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (52/46);  new base T (3/3);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 110 total aligned reads displayed.

ACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATA  >  NC_000913/4470810‑4471106
                                                                                                                                                    |                                                                                                                                                    
aCCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGATCATTATAgg                                                                                                                                                      >  1:911055/1‑149 (MQ=255)
   gggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGAAAGGAGCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGTTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                                                                   <  1:1223448/149‑1 (MQ=255)
       tCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATCGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTATTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATATGGAGCCAg                                                                                                                                               >  1:139076/1‑149 (MQ=255)
        cTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGa                                                                                                                                              <  2:1152357/149‑1 (MQ=255)
        cTGACCGGAGGAGATGATCTTATTGCCCAGATCAACGCCATGTAGGTAAGGACCTATAGCTGCCGGGGCATTTTCCGTCGCGATAGTTTTTCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGCGCCAGa                                                                                                                                              <  1:1458864/149‑1 (MQ=255)
         tGACCGGAGGTGATGATCATATTGCCCCGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCTTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGTGAGCCAGaa                                                                                                                                             >  1:183375/1‑148 (MQ=255)
         tGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                            <  1:1531235/149‑1 (MQ=255)
          gACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                            >  1:1571742/1‑149 (MQ=255)
           aCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtt                                                                                                                                           <  1:292654/149‑1 (MQ=255)
           aCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtt                                                                                                                                           >  2:418958/1‑149 (MQ=255)
            ccGGAGCGGATCATCATATAGCCCAGAACAACGCCAAGTACCTAAGGACCGAAAGCTGCCCGTGCTTTTTCCGTACCGATAGTTTTCCTCATGAATTCTCCTTTATTTAATCTTTAAAATGGCTGGCTCATTATAGGGAGCCAGACttt                                                                                                                                          <  2:858207/149‑1 (MQ=255)
             cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGCTTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCACAGtttt                                                                                                                                         >  1:1378964/1‑149 (MQ=255)
                     atgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCaa                                                                                                                                 >  2:179734/1‑149 (MQ=255)
                       gatCATATTGCCCCGATCAACGCCCTGTACTTAAGGACCGATAGCTGCCGGGGCATTTTCCGTCGCGATAGTTTTGATAATGATGTCACCTTTATTACAGCGGTAAAAAGTCTGGATCATTATAGGGTGCCAGACGTTTATTACCCAcc                                                                                                                               >  2:471569/1‑149 (MQ=255)
                         tCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGc                                                                                                                             >  2:1271163/1‑149 (MQ=255)
                         tCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGc                                                                                                                             >  2:328317/1‑149 (MQ=255)
                            tatTGCCCAGATCAACGCACTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaat                                                                                                                          <  2:1350790/149‑1 (MQ=255)
                             atTGCCCAGATCAACGCCCGGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGGTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                                                                         >  1:1450699/1‑149 (MQ=255)
                                 cccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaatt                                                                                                                     >  1:57998/1‑149 (MQ=255)
                                  ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATACTTTTGCTCATGATTTCTCCTTAATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaattt                                                                                                                    >  2:75604/1‑148 (MQ=255)
                                  ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATATTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                                    >  1:1348067/1‑149 (MQ=255)
                                     gATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc                                                                                                                 >  1:1225715/1‑149 (MQ=255)
                                      aTCGACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGAGAGGTTTGCTCATGATTTCTCCTTTATTACATCGGTAAAAAGTCTGGCTCAGTAGAGGGGGCCAGACGTTGATTACCACGCGCAAGAAATTGGccc                                                                                                                >  2:1078216/1‑148 (MQ=255)
                                          aCGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcac                                                                                                            >  1:694984/1‑149 (MQ=255)
                                          aCGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcac                                                                                                            >  1:574605/1‑149 (MQ=255)
                                             cccTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcaccac                                                                                                         >  1:1315268/1‑149 (MQ=255)
                                              ccTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACcaca                                                                                                        <  2:1335293/149‑1 (MQ=255)
                                                 gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTa                                                                                                     >  1:1186503/1‑149 (MQ=255)
                                                  tACGTAAGGACCGATAGCTGCCGGTGCTTTTTCCGTCGCGATAGTTTTGCTCATGAGGTCGCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGCGCCAGACGTTTATTACCAACCGCAATTAATTGGCCAGCACCACAtttt                                                                                                    >  1:1062976/1‑147 (MQ=255)
                                                      tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGa                                                                                                <  2:434012/149‑1 (MQ=255)
                                                          gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAAc                                                                                            >  2:610593/1‑149 (MQ=255)
                                                             cGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACAGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                         >  1:844547/1‑149 (MQ=255)
                                                             cGACAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                         >  2:1089734/1‑149 (MQ=255)
                                                               aTAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttt                                                                                       >  1:783351/1‑149 (MQ=255)
                                                                 aGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCTCATTATGGGAAAACTCttttt                                                                                     <  1:541038/149‑1 (MQ=255)
                                                                 aGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttttt                                                                                     <  2:24440/149‑1 (MQ=255)
                                                                  gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTc                                                                                    <  1:1230444/149‑1 (MQ=255)
                                                                   cttccGGTACATTTTCCGTCGCGATAGATTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCTTTATAGGGAGCCAGACTTTTATTACCAACCGGAATTTATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                                   <  1:518167/146‑1 (MQ=255)
                                                                   cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                                   <  2:779764/149‑1 (MQ=255)
                                                                   cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                                   >  2:54613/1‑149 (MQ=255)
                                                                     gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTgaca                                                                                 >  1:1417939/1‑149 (MQ=255)
                                                                     gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcaca                                                                                 <  1:441384/149‑1 (MQ=255)
                                                                     gCCGGTGCATTTACCGTCGCGATAGTTTTGCTCATGATTTCTCCTATATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcaca                                                                                 <  2:196949/149‑1 (MQ=255)
                                                                       cGGGGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGt                                                                               <  1:1537996/149‑1 (MQ=255)
                                                                         gTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCACCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAt                                                                             >  1:1404301/1‑149 (MQ=255)
                                                                          tGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAtt                                                                            >  2:114056/1‑149 (MQ=255)
                                                                           gCATTTTCCGTCGCGATAGTTTTGCTCCTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAttt                                                                           <  2:1197718/149‑1 (MQ=255)
                                                                           gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAttt                                                                           <  1:1338533/149‑1 (MQ=255)
                                                                           gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAttt                                                                           <  2:590056/149‑1 (MQ=255)
                                                                            cATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCCACCGCAATTAATTGGCCATCACAACATAATGGGATCAATCTTTTTCACAGTAttag                                                                          >  2:14658/1‑147 (MQ=255)
                                                                               tttCCGTCGCTATAGTTTTTCTCCTGATTTCTCCTTTATTTCATCGGTAAAAATTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATAGGAAAACTCTTTTTCACAGTATTTGCAt                                                                       <  2:1126677/149‑1 (MQ=255)
                                                                                ttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAtt                                                                      <  1:418958/149‑1 (MQ=255)
                                                                                    gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg                                                                  >  1:1196340/1‑149 (MQ=255)
                                                                                      cgcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcg                                                                <  2:828791/149‑1 (MQ=255)
                                                                                       gcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCGCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc                                                               <  2:1461328/149‑1 (MQ=255)
                                                                                            aGTTTTGCTCATGATTTCTCCTTTATTACATCCGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACAGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat                                                          <  2:1164695/149‑1 (MQ=255)
                                                                                            aGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat                                                          >  2:431338/1‑149 (MQ=255)
                                                                                               tttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt                                                       <  1:1271163/149‑1 (MQ=255)
                                                                                                ttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt                                                      <  1:903683/149‑1 (MQ=255)
                                                                                                 tGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTg                                                     <  1:585028/149‑1 (MQ=255)
                                                                                                   cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATATGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc                                                   <  2:1173939/149‑1 (MQ=255)
                                                                                                   cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc                                                   <  1:1389485/149‑1 (MQ=255)
                                                                                                   cTCATGATTTATCCTTTATTACAGCGGTAAAAAGTCTGGCTAATTATAGAGAGCCAGACTTTTATTACCAGCCGCAATTAATAGGCCAGCACAACAGTATGGGTACACTCGTTGTCGCCGTATGTGCAGTTGAGCGCGATATCATTGgc                                                   >  2:1063909/1‑149 (MQ=255)
                                                                                                    tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCGAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTCAGCGCGATATCATTGgcg                                                  >  2:1463764/1‑149 (MQ=255)
                                                                                                    tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg                                                  >  1:797484/1‑149 (MQ=255)
                                                                                                    tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg                                                  >  1:140749/1‑149 (MQ=255)
                                                                                                    tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg                                                  >  2:1429287/1‑149 (MQ=255)
                                                                                                      aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCTCCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcgct                                                >  2:458485/1‑148 (MQ=255)
                                                                                                      aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGcgcg                                                >  1:740924/1‑149 (MQ=255)
                                                                                                      aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGCcgcg                                                >  2:407974/1‑149 (MQ=255)
                                                                                                      aTGAATTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGAGTTTTATTAGCTACCGCAATTAATTGGACAGAAACACATTATGGGAAAACTATTTTTCAGTGTATTTGAGTTTGTGGGCGATTTCTTTGgcggg                                                >  2:1083627/1‑147 (MQ=255)
                                                                                                           ttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc                                           <  2:1315268/149‑1 (MQ=255)
                                                                                                              tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                        <  2:1316884/149‑1 (MQ=255)
                                                                                                                cgatcttaCCGCGCAAAAAAGTCAGGATCATAATAGCGAGCCAGACTTGTCTTCCCCCCCGCACTTACTTGGCCCCCACCACCTTCGGGGACCACTCTTTTTCCCAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg                                      <  2:1044076/144‑1 (MQ=255)
                                                                                                                cTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                                                                    <  1:1002430/119‑1 (MQ=255)
                                                                                                                cTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                                                                    >  2:1002430/1‑119 (MQ=255)
                                                                                                                cTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg                                      >  2:351970/1‑149 (MQ=255)
                                                                                                                    attaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaa                                  >  1:1103061/1‑149 (MQ=255)
                                                                                                                        cAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCt                              <  1:114056/149‑1 (MQ=255)
                                                                                                                         aGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg                             >  2:1078453/1‑149 (MQ=255)
                                                                                                                         aGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg                             >  2:43513/1‑149 (MQ=255)
                                                                                                                            ggTAAAATGTCTGGATCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt                          <  2:183375/149‑1 (MQ=255)
                                                                                                                                aaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa                      >  2:379819/1‑149 (MQ=255)
                                                                                                                                 aaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAc                     <  1:1078453/149‑1 (MQ=255)
                                                                                                                                  aaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc                    >  1:701146/1‑149 (MQ=255)
                                                                                                                                  aaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc                    <  1:1413334/149‑1 (MQ=255)
                                                                                                                                      cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt                >  1:765976/1‑149 (MQ=255)
                                                                                                                                       tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc               >  1:430882/1‑149 (MQ=255)
                                                                                                                                         gCTCATTATAGGGCGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTTGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCTGTTCgg             >  1:1054326/1‑149 (MQ=255)
                                                                                                                                         gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg             <  2:694984/149‑1 (MQ=255)
                                                                                                                                          cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc            <  2:1363065/149‑1 (MQ=255)
                                                                                                                                          cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc            >  2:834094/1‑149 (MQ=255)
                                                                                                                                           tCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa           >  2:1210154/1‑149 (MQ=255)
                                                                                                                                            cATTATAGGGCGGCAGACGTTTATTACCAACCGCAATTAATTGGCCAGCACCCCATAATCGGAAAACTCTTTTTCCCAGTATTTGCATCTGCGCGCGATATCAATGGCGCGGTTTCGCACGGCAACGCTGGATGAAACCGGTTCGGCAt          <  2:1382558/149‑1 (MQ=255)
                                                                                                                                            cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAt          >  2:225454/1‑149 (MQ=255)
                                                                                                                                              ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGCTCGGCATgg        <  2:844547/149‑1 (MQ=255)
                                                                                                                                               tataGGGAGCCAGACTTTTATTACCAACAGCAATTAATTGGCCAGCACAACATTATGGTAAAACTCTTTTTCACAGTATTTGCACTTGAGAGCGATATCATTGGCGAGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc       <  1:1050550/149‑1 (MQ=255)
                                                                                                                                                 taGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTCCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTg     >  1:107369/1‑149 (MQ=255)
                                                                                                                                                    ggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGCTATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa  >  2:1285791/1‑149 (MQ=255)
                                                                                                                                                    ggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa  >  2:56164/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATA  >  NC_000913/4470810‑4471106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: